NUP205

PF3D7_1364200 (NUP205) encodes a nucleoporin of the Plasmodium nuclear pore complex (NPC), localizing to the nuclear periphery throughout the parasite life cycle in the P. berghei ortholog (PMID:30050042). Proximity labeling establishes NUP205 as a physical constituent of the NPC and a proximal interactor of the nucleoporin NUP313, being biotinylated and recovered by mass spectrometry from a NUP313::TurboID strain (PMID:36040030). Its peripheral nuclear distribution is maintained at the late trophozoite stage, where it serves as a nuclear-periphery marker alongside NAB2 (PMID:34604117). Beyond its membership in the NPC and its proximity to NUP313, no enzymatic activity, direct nucleocytoplasmic transport role, or loss-of-function phenotype has been characterized for NUP205 itself in the available corpus.

1. 2018 Medium

   Establishing whether NUP205 is a genuine nuclear pore component was the first question; bioinformatic prediction plus fluorescent tagging placed it at the nuclear periphery across the life cycle, defining it as a bona fide nucleoporin rather than a predicted homolog only.

   Evidence Bioinformatic identification with fluorescent protein tagging and life-cycle localization imaging in P. berghei

   PMID:30050042

   Open questions at the time

   • No functional assay or loss-of-function phenotype for NUP205
   • No demonstration of a direct transport or scaffolding role
   • Assignment based on the P. berghei ortholog, not the P. falciparum gene directly
2. 2021 Medium

   Whether NUP205 retains its peripheral localization in specific asexual stages was addressed by GFP tagging, confirming nuclear-periphery residence at the late trophozoite stage and validating its use as a positional marker for co-localization.

   Evidence Live-cell fluorescence imaging of endogenously GFP-tagged NUP205 co-expressed with NAB2::mCherry in P. berghei ANKA

   PMID:34604117

   Open questions at the time

   • Localization does not establish molecular function
   • No evidence of dynamic behavior or stage-specific regulation of NUP205
3. 2022 Medium

   The question of NUP205's physical context within the NPC was answered by proximity labeling, which placed it in close spatial proximity to NUP313, providing orthogonal biochemical confirmation of NPC membership beyond imaging.

   Evidence TurboID proximity labeling with affinity purification and mass spectrometry, anti-HA immunofluorescence, and streptavidin staining in P. berghei

   PMID:36040030

   Open questions at the time

   • Proximity does not establish a direct binary interaction or complex architecture
   • No stoichiometry or assembly ordering within the NPC defined
   • No functional consequence of the NUP205–NUP313 association tested

• The molecular function of NUP205 — whether it acts as a structural scaffold, a transport-competent FG-nucleoporin, or has a regulatory role — and any loss-of-function phenotype remain undefined in the corpus.
• No gene disruption or conditional knockdown of NUP205 reported
• No enzymatic or transport activity assigned
• No structural model of NUP205 within the Plasmodium NPC
• All evidence derives from the P. berghei ortholog rather than PF3D7_1364200 directly