{"gene":"PF3D7_1364200","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2018,"finding":"NUP205 (PF3D7_1364200 ortholog in P. berghei) was identified as a nucleoporin component of the nuclear pore complex and localized to the nuclear periphery throughout the P. berghei life cycle, using bioinformatic identification combined with fluorescent protein tagging.","method":"Bioinformatic identification combined with fluorescent protein tagging and localization imaging throughout the parasite life cycle","journal":"Scientific reports","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — direct localization by fluorescent tagging, single lab, but confirmed across life cycle stages; no functional epistasis or in vitro assay","pmids":["30050042"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2022,"finding":"NUP205 (P. berghei ortholog) was confirmed as a component of the nuclear pore complex by TurboID proximity labeling: Nup205::TurboID-HA fusion protein localized to the nuclear periphery by anti-HA immunofluorescence and streptavidin staining, and was identified in mass spectrometric analysis of affinity-purified biotinylated proteins from a Nup313::TurboID strain, establishing NUP205 as a proximal interactor of NUP313 within the NPC.","method":"TurboID proximity labeling with affinity purification and mass spectrometry; anti-HA immunofluorescence; streptavidin staining","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — proximity labeling MS plus direct fluorescence localization, single lab, two orthogonal methods","pmids":["36040030"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2021,"finding":"NUP205 (P. berghei ortholog PBANKA_1140100) localizes to the nuclear periphery in the late trophozoite stage, as determined by live-cell fluorescence imaging of a GFP-tagged NUP205 fusion protein co-expressed with NAB2::mCherry, which itself localizes to the nuclear periphery.","method":"Live-cell fluorescence imaging of endogenously GFP-tagged NUP205 in transgenic P. berghei","journal":"Frontiers in cellular and infection microbiology","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — direct localization by live-cell imaging with GFP tag, single lab, used as a nuclear periphery marker in co-localization context","pmids":["34604117"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":"ANKA"}]}],"current_model":"PF3D7_1364200 (NUP205) encodes a nucleoporin that localizes to the nuclear periphery in Plasmodium parasites and is a component of the nuclear pore complex, where it physically associates with other FG-nucleoporins (including NUP313) as established by proximity labeling and fluorescent tagging; no enzymatic activity, direct transport role, or loss-of-function phenotype has yet been experimentally defined for this specific protein."},"narrative":{"mechanistic_narrative":"PF3D7_1364200 (NUP205) encodes a nucleoporin of the Plasmodium nuclear pore complex (NPC), localizing to the nuclear periphery throughout the parasite life cycle in the P. berghei ortholog [PMID:30050042]. Proximity labeling establishes NUP205 as a physical constituent of the NPC and a proximal interactor of the nucleoporin NUP313, being biotinylated and recovered by mass spectrometry from a NUP313::TurboID strain [PMID:36040030]. Its peripheral nuclear distribution is maintained at the late trophozoite stage, where it serves as a nuclear-periphery marker alongside NAB2 [PMID:34604117]. Beyond its membership in the NPC and its proximity to NUP313, no enzymatic activity, direct nucleocytoplasmic transport role, or loss-of-function phenotype has been characterized for NUP205 itself in the available corpus.","teleology":[{"year":2018,"claim":"Establishing whether NUP205 is a genuine nuclear pore component was the first question; bioinformatic prediction plus fluorescent tagging placed it at the nuclear periphery across the life cycle, defining it as a bona fide nucleoporin rather than a predicted homolog only.","evidence":"Bioinformatic identification with fluorescent protein tagging and life-cycle localization imaging in P. berghei","pmids":["30050042"],"confidence":"Medium","gaps":["No functional assay or loss-of-function phenotype for NUP205","No demonstration of a direct transport or scaffolding role","Assignment based on the P. berghei ortholog, not the P. falciparum gene directly"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2021,"claim":"Whether NUP205 retains its peripheral localization in specific asexual stages was addressed by GFP tagging, confirming nuclear-periphery residence at the late trophozoite stage and validating its use as a positional marker for co-localization.","evidence":"Live-cell fluorescence imaging of endogenously GFP-tagged NUP205 co-expressed with NAB2::mCherry in P. berghei ANKA","pmids":["34604117"],"confidence":"Medium","gaps":["Localization does not establish molecular function","No evidence of dynamic behavior or stage-specific regulation of NUP205"],"attribution":[{"species":"Plasmodium berghei","strain":"ANKA"}]},{"year":2022,"claim":"The question of NUP205's physical context within the NPC was answered by proximity labeling, which placed it in close spatial proximity to NUP313, providing orthogonal biochemical confirmation of NPC membership beyond imaging.","evidence":"TurboID proximity labeling with affinity purification and mass spectrometry, anti-HA immunofluorescence, and streptavidin staining in P. berghei","pmids":["36040030"],"confidence":"Medium","gaps":["Proximity does not establish a direct binary interaction or complex architecture","No stoichiometry or assembly ordering within the NPC defined","No functional consequence of the NUP205–NUP313 association tested"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The molecular function of NUP205 — whether it acts as a structural scaffold, a transport-competent FG-nucleoporin, or has a regulatory role — and any loss-of-function phenotype remain undefined in the corpus.","evidence":"","pmids":[],"confidence":"Low","gaps":["No gene disruption or conditional knockdown of NUP205 reported","No enzymatic or transport activity assigned","No structural model of NUP205 within the Plasmodium NPC","All evidence derives from the P. berghei ortholog rather than PF3D7_1364200 directly"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Nuclear pore complex (NPC)"],"partners":["NUP313 (PROXIMAL INTERACTOR BY TURBOID PROXIMITY LABELING)"],"other_free_text":["All experimental evidence derives from the P. berghei ortholog (PBANKA_1140100); no direct functional characterization of P. falciparum PF3D7_1364200 is present in the corpus.","NUP205 localizes to the nuclear periphery throughout the P. berghei life cycle and at the late trophozoite stage.","No loss-of-function phenotype, enzymatic activity, or direct transport role has been established for NUP205."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"30050042","id":"PMC_30050042","title":"Nuclear Pore Complex Components in the Malaria Parasite Plasmodium berghei.","date":"2018","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/30050042","citation_count":20,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41885428","id":"","title":"A heterologous marker-free selection approach for CRISPR/Cas9-based gene editing in the malaria parasite Plasmodium falciparum.","date":"2026","source":"mSphere","url":"https://pubmed.ncbi.nlm.nih.gov/41885428","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["NUP205"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36040030","id":"","title":"TurboID Identification of Evolutionarily Divergent Components of the Nuclear Pore Complex in the Malaria Model Plasmodium berghei.","date":"2022","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/36040030","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["NUP205"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34604117","id":"","title":"Roles and Cellular Localization of GBP2 and NAB2 During the Blood Stage of Malaria Parasites.","date":"2021","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/34604117","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["NUP205"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":7793,"output_tokens":924,"usd":0.01862,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5658,"output_tokens":1871,"usd":0.037533,"stage2_stop_reason":"end_turn"},"total_usd":0.056153,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2018,\n      \"finding\": \"NUP205 (PF3D7_1364200 ortholog in P. berghei) was identified as a nucleoporin component of the nuclear pore complex and localized to the nuclear periphery throughout the P. berghei life cycle, using bioinformatic identification combined with fluorescent protein tagging.\",\n      \"method\": \"Bioinformatic identification combined with fluorescent protein tagging and localization imaging throughout the parasite life cycle\",\n      \"journal\": \"Scientific reports\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — direct localization by fluorescent tagging, single lab, but confirmed across life cycle stages; no functional epistasis or in vitro assay\",\n      \"pmids\": [\"30050042\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"NUP205 (P. berghei ortholog) was confirmed as a component of the nuclear pore complex by TurboID proximity labeling: Nup205::TurboID-HA fusion protein localized to the nuclear periphery by anti-HA immunofluorescence and streptavidin staining, and was identified in mass spectrometric analysis of affinity-purified biotinylated proteins from a Nup313::TurboID strain, establishing NUP205 as a proximal interactor of NUP313 within the NPC.\",\n      \"method\": \"TurboID proximity labeling with affinity purification and mass spectrometry; anti-HA immunofluorescence; streptavidin staining\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — proximity labeling MS plus direct fluorescence localization, single lab, two orthogonal methods\",\n      \"pmids\": [\"36040030\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"NUP205 (P. berghei ortholog PBANKA_1140100) localizes to the nuclear periphery in the late trophozoite stage, as determined by live-cell fluorescence imaging of a GFP-tagged NUP205 fusion protein co-expressed with NAB2::mCherry, which itself localizes to the nuclear periphery.\",\n      \"method\": \"Live-cell fluorescence imaging of endogenously GFP-tagged NUP205 in transgenic P. berghei\",\n      \"journal\": \"Frontiers in cellular and infection microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — direct localization by live-cell imaging with GFP tag, single lab, used as a nuclear periphery marker in co-localization context\",\n      \"pmids\": [\"34604117\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"ANKA\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_1364200 (NUP205) encodes a nucleoporin that localizes to the nuclear periphery in Plasmodium parasites and is a component of the nuclear pore complex, where it physically associates with other FG-nucleoporins (including NUP313) as established by proximity labeling and fluorescent tagging; no enzymatic activity, direct transport role, or loss-of-function phenotype has yet been experimentally defined for this specific protein.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1364200 (NUP205) encodes a nucleoporin of the Plasmodium nuclear pore complex (NPC), localizing to the nuclear periphery throughout the parasite life cycle in the P. berghei ortholog [#0]. Proximity labeling establishes NUP205 as a physical constituent of the NPC and a proximal interactor of the nucleoporin NUP313, being biotinylated and recovered by mass spectrometry from a NUP313::TurboID strain [#1]. Its peripheral nuclear distribution is maintained at the late trophozoite stage, where it serves as a nuclear-periphery marker alongside NAB2 [#2]. Beyond its membership in the NPC and its proximity to NUP313, no enzymatic activity, direct nucleocytoplasmic transport role, or loss-of-function phenotype has been characterized for NUP205 itself in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2018,\n      \"claim\": \"Establishing whether NUP205 is a genuine nuclear pore component was the first question; bioinformatic prediction plus fluorescent tagging placed it at the nuclear periphery across the life cycle, defining it as a bona fide nucleoporin rather than a predicted homolog only.\",\n      \"evidence\": \"Bioinformatic identification with fluorescent protein tagging and life-cycle localization imaging in P. berghei\",\n      \"pmids\": [\"30050042\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional assay or loss-of-function phenotype for NUP205\",\n        \"No demonstration of a direct transport or scaffolding role\",\n        \"Assignment based on the P. berghei ortholog, not the P. falciparum gene directly\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"Whether NUP205 retains its peripheral localization in specific asexual stages was addressed by GFP tagging, confirming nuclear-periphery residence at the late trophozoite stage and validating its use as a positional marker for co-localization.\",\n      \"evidence\": \"Live-cell fluorescence imaging of endogenously GFP-tagged NUP205 co-expressed with NAB2::mCherry in P. berghei ANKA\",\n      \"pmids\": [\"34604117\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Localization does not establish molecular function\",\n        \"No evidence of dynamic behavior or stage-specific regulation of NUP205\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"ANKA\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"The question of NUP205's physical context within the NPC was answered by proximity labeling, which placed it in close spatial proximity to NUP313, providing orthogonal biochemical confirmation of NPC membership beyond imaging.\",\n      \"evidence\": \"TurboID proximity labeling with affinity purification and mass spectrometry, anti-HA immunofluorescence, and streptavidin staining in P. berghei\",\n      \"pmids\": [\"36040030\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Proximity does not establish a direct binary interaction or complex architecture\",\n        \"No stoichiometry or assembly ordering within the NPC defined\",\n        \"No functional consequence of the NUP205–NUP313 association tested\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function of NUP205 — whether it acts as a structural scaffold, a transport-competent FG-nucleoporin, or has a regulatory role — and any loss-of-function phenotype remain undefined in the corpus.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No gene disruption or conditional knockdown of NUP205 reported\",\n        \"No enzymatic or transport activity assigned\",\n        \"No structural model of NUP205 within the Plasmodium NPC\",\n        \"All evidence derives from the P. berghei ortholog rather than PF3D7_1364200 directly\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0017056\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005643\", \"supporting_discovery_ids\": [0, 1, 2]},\n      {\"term_id\": \"GO:0034399\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"Nuclear pore complex (NPC)\"],\n    \"partners\": [\"NUP313 (proximal interactor by TurboID proximity labeling)\"],\n    \"other_free_text\": [\n      \"All experimental evidence derives from the P. berghei ortholog (PBANKA_1140100); no direct functional characterization of P. falciparum PF3D7_1364200 is present in the corpus.\",\n      \"NUP205 localizes to the nuclear periphery throughout the P. berghei life cycle and at the late trophozoite stage.\",\n      \"No loss-of-function phenotype, enzymatic activity, or direct transport role has been established for NUP205.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1364200","name":null,"product":"nucleoporin NUP205, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1140100","name":null,"product":"nucleoporin NUP205","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1107100","name":null,"product":"nucleoporin NUP205, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"NUP205","evaluation":null}