ATPTG15

PF3D7_0825400 (ATPTG15) is a putative ATP synthase-associated subunit of the Plasmodium falciparum mitochondrion. It was localized to the parasite mitochondrion as one of seven epitope-tagged canonical ATP synthase subunits, and blue native gel electrophoresis of mitochondrial membranes established that the ATP synthase complex it belongs to exceeds 1 MDa, consistent with a dimeric assembly and implying subunits beyond the canonical set (PMID:21984828). Beyond its membership in this large mitochondrial ATP synthase complex, no subunit-specific enzymatic activity, interaction, or functional role has been experimentally established for PF3D7_0825400 in the available corpus.

1. 2011 Medium

   It was unknown whether the canonical ATP synthase subunits of P. falciparum, including the product of PF3D7_0825400, are mitochondrial and assemble into a defined complex; epitope-tagging placed the protein in the mitochondrion and native gels showed it belongs to a >1 MDa dimeric complex, establishing its physical context.

   Evidence Epitope-tagging with immunofluorescence localization and blue native gel electrophoresis of mitochondrial membrane fractions in transgenic P. falciparum

   PMID:21984828

   Open questions at the time

   • No subunit-specific function or enzymatic contribution of PF3D7_0825400 was dissected; it was studied only collectively among seven subunits
   • Direct interaction partners within the complex were not resolved
   • Whether PF3D7_0825400 is required for complex assembly or activity was not tested

• The specific molecular role of PF3D7_0825400 within the divergent Plasmodium ATP synthase complex — its enzymatic contribution, its direct partner subunits, and its requirement for complex assembly or activity — remains uncharacterized in the available corpus.
• No subunit-specific interaction data
• No structural placement within the complex
• No genetic knockout or knockdown phenotype for PF3D7_0825400 itself