AKIT10

AKIT10 (PF3D7_0307700) is a component of the apicomplexan kinetochore complex, identified as one of 13 previously unknown kinetochore proteins in Apicomplexa through interactomics combined with deep homology searches in Plasmodium falciparum (PMID:36006241). Beyond this membership in the kinetochore complex, no further mechanistic detail about the specific molecular function of AKIT10 has been characterized in the available corpus.

1. 2022 Medium

   The composition of the apicomplexan kinetochore was largely unknown; combining interactomics with deep homology searches revealed AKIT10 as one of 13 previously unrecognized kinetochore components, establishing that it is a bona fide member of this complex.

   Evidence Interactomics (co-immunoprecipitation/mass spectrometry) with deep homology searches and subkinetochore localization assays in P. falciparum

   PMID:36006241

   Open questions at the time

   • No direct functional mutagenesis or reconstitution of AKIT10 specifically was reported
   • The specific molecular activity of AKIT10 within the kinetochore is undefined
   • Direct binding partners and structural position of AKIT10 within the complex are not resolved

• What specific molecular function AKIT10 performs within the apicomplexan kinetochore, and whether it is essential for chromosome segregation, remains unknown.
• No dedicated AKIT10 loss-of-function or complementation study
• No identified direct interaction partners or substrate
• No structural model of AKIT10 or its placement in the complex