{"gene":"PF3D7_0307700","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2022,"finding":"PF3D7_0307700 (AKIT10) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, establishing its membership in the apicomplexan kinetochore complex.","method":"Interactomics (co-immunoprecipitation/mass spectrometry) combined with deep homology searches; subkinetochore localization assays","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — interactomics-based identification in a single study; no direct functional mutagenesis or reconstitution of AKIT10 specifically reported in the abstract","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"AKIT10 (PF3D7_0307700) is a component of the apicomplexan kinetochore complex, identified via interactomics and deep homology searches as one of 13 novel kinetochore proteins in Apicomplexa; beyond its membership in this complex, no further mechanistic details about its specific molecular function have been established."},"narrative":{"mechanistic_narrative":"AKIT10 (PF3D7_0307700) is a component of the apicomplexan kinetochore complex, identified as one of 13 previously unknown kinetochore proteins in Apicomplexa through interactomics combined with deep homology searches in Plasmodium falciparum [PMID:36006241]. Beyond this membership in the kinetochore complex, no further mechanistic detail about the specific molecular function of AKIT10 has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"The composition of the apicomplexan kinetochore was largely unknown; combining interactomics with deep homology searches revealed AKIT10 as one of 13 previously unrecognized kinetochore components, establishing that it is a bona fide member of this complex.","evidence":"Interactomics (co-immunoprecipitation/mass spectrometry) with deep homology searches and subkinetochore localization assays in P. falciparum","pmids":["36006241"],"confidence":"Medium","gaps":["No direct functional mutagenesis or reconstitution of AKIT10 specifically was reported","The specific molecular activity of AKIT10 within the kinetochore is undefined","Direct binding partners and structural position of AKIT10 within the complex are not resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"What specific molecular function AKIT10 performs within the apicomplexan kinetochore, and whether it is essential for chromosome segregation, remains unknown.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No dedicated AKIT10 loss-of-function or complementation study","No identified direct interaction partners or substrate","No structural model of AKIT10 or its placement in the complex"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["apicomplexan kinetochore complex"],"partners":[],"other_free_text":["Identified as one of 13 novel apicomplexan kinetochore components; beyond membership in this complex, no gene-specific molecular function has been established for AKIT10 in the corpus."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":31,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3258,"output_tokens":362,"usd":0.007602,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4832,"output_tokens":932,"usd":0.02373,"stage2_stop_reason":"end_turn"},"total_usd":0.031332,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"PF3D7_0307700 (AKIT10) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, establishing its membership in the apicomplexan kinetochore complex.\",\n      \"method\": \"Interactomics (co-immunoprecipitation/mass spectrometry) combined with deep homology searches; subkinetochore localization assays\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — interactomics-based identification in a single study; no direct functional mutagenesis or reconstitution of AKIT10 specifically reported in the abstract\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"AKIT10 (PF3D7_0307700) is a component of the apicomplexan kinetochore complex, identified via interactomics and deep homology searches as one of 13 novel kinetochore proteins in Apicomplexa; beyond its membership in this complex, no further mechanistic details about its specific molecular function have been established.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AKIT10 (PF3D7_0307700) is a component of the apicomplexan kinetochore complex, identified as one of 13 previously unknown kinetochore proteins in Apicomplexa through interactomics combined with deep homology searches in Plasmodium falciparum [#0]. Beyond this membership in the kinetochore complex, no further mechanistic detail about the specific molecular function of AKIT10 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"The composition of the apicomplexan kinetochore was largely unknown; combining interactomics with deep homology searches revealed AKIT10 as one of 13 previously unrecognized kinetochore components, establishing that it is a bona fide member of this complex.\",\n      \"evidence\": \"Interactomics (co-immunoprecipitation/mass spectrometry) with deep homology searches and subkinetochore localization assays in P. falciparum\",\n      \"pmids\": [\"36006241\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct functional mutagenesis or reconstitution of AKIT10 specifically was reported\",\n        \"The specific molecular activity of AKIT10 within the kinetochore is undefined\",\n        \"Direct binding partners and structural position of AKIT10 within the complex are not resolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"What specific molecular function AKIT10 performs within the apicomplexan kinetochore, and whether it is essential for chromosome segregation, remains unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No dedicated AKIT10 loss-of-function or complementation study\",\n        \"No identified direct interaction partners or substrate\",\n        \"No structural model of AKIT10 or its placement in the complex\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0000776\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"apicomplexan kinetochore complex\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as one of 13 novel apicomplexan kinetochore components; beyond membership in this complex, no gene-specific molecular function has been established for AKIT10 in the corpus.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0307700","name":null,"product":"apicomplexan kinetochore protein 10, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0406000","name":null,"product":"conserved Plasmodium protein, unknown function","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0835000","name":null,"product":"apicomplexan kinetochore protein 10, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AKIT10","evaluation":null}