GET2

PF3D7_0215600 (GET2) is the Plasmodium falciparum homolog of yeast Get2 and associates in vitro with PfGet3, placing it as a component of the Guided Entry of Tail-Anchored (GET) protein trafficking pathway in the parasite (PMID:34780541). Beyond this physical association with PfGet3 (PMID:34780541), no further mechanistic detail — including membrane topology, substrate specificity, or in vivo function of GET2 itself — has been characterized in the available corpus.

1. 2021 Low

   Whether P. falciparum encodes a functional Get2 component that engages the GET pathway machinery was unknown; demonstrating a physical association between the PF3D7_0215600 gene product and PfGet3 established GET2 as part of the parasite's tail-anchored protein trafficking system.

   Evidence In vitro association/pulldown assay between PfGet3 and the P. falciparum Get2 homolog (PF3D7_0215600)

   PMID:34780541

   Open questions at the time

   • Single in vitro association method without mutagenesis, reciprocal validation, or structural characterization
   • No demonstration of GET2 function or requirement in living parasites
   • Substrate range and membrane topology of GET2 not defined

• It remains unknown how GET2 contributes mechanistically to tail-anchored protein insertion in P. falciparum, including its localization, its role in receiving substrate from PfGet3, and its importance for parasite viability.
• No genetic knockout or knockdown phenotype for GET2
• No structural or topological model of the GET2 protein
• No identified tail-anchored substrates delivered via GET2