{"gene":"PF3D7_0215600","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2021,"finding":"PF3D7_0215600 (GET2), the P. falciparum homolog of Get2, was shown by in vitro association studies to interact with PfGet3, establishing GET2 as a component of the Guided Entry of Tail-Anchored (GET) protein trafficking pathway in P. falciparum.","method":"In vitro association assay (pulldown/binding study) between PfGet3 and the P. falciparum Get2 homolog (PF3D7_0215600)","journal":"PLoS Pathogens","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single lab, single in vitro association method, no mutagenesis or structural validation; abstract does not specify detailed controls","pmids":["34780541"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_0215600 (GET2) is a P. falciparum homolog of yeast Get2 that physically associates with PfGet3 in vitro, placing it as a component of the conserved Guided Entry of Tail-Anchored (GET) protein trafficking pathway responsible for post-translational membrane insertion of tail-anchored proteins in the parasite."},"narrative":{"mechanistic_narrative":"PF3D7_0215600 (GET2) is the Plasmodium falciparum homolog of yeast Get2 and associates in vitro with PfGet3, placing it as a component of the Guided Entry of Tail-Anchored (GET) protein trafficking pathway in the parasite [PMID:34780541]. Beyond this physical association with PfGet3 [PMID:34780541], no further mechanistic detail — including membrane topology, substrate specificity, or in vivo function of GET2 itself — has been characterized in the available corpus.","teleology":[{"year":2021,"claim":"Whether P. falciparum encodes a functional Get2 component that engages the GET pathway machinery was unknown; demonstrating a physical association between the PF3D7_0215600 gene product and PfGet3 established GET2 as part of the parasite's tail-anchored protein trafficking system.","evidence":"In vitro association/pulldown assay between PfGet3 and the P. falciparum Get2 homolog (PF3D7_0215600)","pmids":["34780541"],"confidence":"Low","gaps":["Single in vitro association method without mutagenesis, reciprocal validation, or structural characterization","No demonstration of GET2 function or requirement in living parasites","Substrate range and membrane topology of GET2 not defined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"It remains unknown how GET2 contributes mechanistically to tail-anchored protein insertion in P. falciparum, including its localization, its role in receiving substrate from PfGet3, and its importance for parasite viability.","evidence":"","pmids":[],"confidence":"Low","gaps":["No genetic knockout or knockdown phenotype for GET2","No structural or topological model of the GET2 protein","No identified tail-anchored substrates delivered via GET2"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Guided Entry of Tail-Anchored (GET) protein trafficking pathway"],"partners":["PFGET3"],"other_free_text":["PF3D7_0215600 (GET2) is the P. falciparum homolog of yeast Get2 and associates with PfGet3 in vitro [#0]; the corpus establishes only its membership in the GET pathway via this interaction and no autonomous function for GET2 itself."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"34780541","id":"PMC_34780541","title":"A conserved guided entry of tail-anchored pathway is involved in the trafficking of a subset of membrane proteins in Plasmodium falciparum.","date":"2021","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/34780541","citation_count":6,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["GET2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3306,"output_tokens":377,"usd":0.007787,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4849,"output_tokens":980,"usd":0.024372,"stage2_stop_reason":"end_turn"},"total_usd":0.032159,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2021,\n      \"finding\": \"PF3D7_0215600 (GET2), the P. falciparum homolog of Get2, was shown by in vitro association studies to interact with PfGet3, establishing GET2 as a component of the Guided Entry of Tail-Anchored (GET) protein trafficking pathway in P. falciparum.\",\n      \"method\": \"In vitro association assay (pulldown/binding study) between PfGet3 and the P. falciparum Get2 homolog (PF3D7_0215600)\",\n      \"journal\": \"PLoS Pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab, single in vitro association method, no mutagenesis or structural validation; abstract does not specify detailed controls\",\n      \"pmids\": [\"34780541\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_0215600 (GET2) is a P. falciparum homolog of yeast Get2 that physically associates with PfGet3 in vitro, placing it as a component of the conserved Guided Entry of Tail-Anchored (GET) protein trafficking pathway responsible for post-translational membrane insertion of tail-anchored proteins in the parasite.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0215600 (GET2) is the Plasmodium falciparum homolog of yeast Get2 and associates in vitro with PfGet3, placing it as a component of the Guided Entry of Tail-Anchored (GET) protein trafficking pathway in the parasite [#0]. Beyond this physical association with PfGet3 [#0], no further mechanistic detail — including membrane topology, substrate specificity, or in vivo function of GET2 itself — has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2021,\n      \"claim\": \"Whether P. falciparum encodes a functional Get2 component that engages the GET pathway machinery was unknown; demonstrating a physical association between the PF3D7_0215600 gene product and PfGet3 established GET2 as part of the parasite's tail-anchored protein trafficking system.\",\n      \"evidence\": \"In vitro association/pulldown assay between PfGet3 and the P. falciparum Get2 homolog (PF3D7_0215600)\",\n      \"pmids\": [\"34780541\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single in vitro association method without mutagenesis, reciprocal validation, or structural characterization\",\n        \"No demonstration of GET2 function or requirement in living parasites\",\n        \"Substrate range and membrane topology of GET2 not defined\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown how GET2 contributes mechanistically to tail-anchored protein insertion in P. falciparum, including its localization, its role in receiving substrate from PfGet3, and its importance for parasite viability.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No genetic knockout or knockdown phenotype for GET2\",\n        \"No structural or topological model of the GET2 protein\",\n        \"No identified tail-anchored substrates delivered via GET2\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [\"Guided Entry of Tail-Anchored (GET) protein trafficking pathway\"],\n    \"partners\": [\"PfGet3\"],\n    \"other_free_text\": [\n      \"PF3D7_0215600 (GET2) is the P. falciparum homolog of yeast Get2 and associates with PfGet3 in vitro [#0]; the corpus establishes only its membership in the GET pathway via this interaction and no autonomous function for GET2 itself.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0215600","name":null,"product":"Golgi to ER traffic protein 2","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0312300","name":null,"product":"conserved Plasmodium protein, unknown function","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0405300","name":null,"product":"Golgi to ER traffic protein 2, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"GET2","evaluation":null}