PBANKA_0506100

PBANKA_0506100 is a PHAX domain-containing protein of Plasmodium berghei identified as an interaction partner of GBP2 by IP-MS, placing it in the vicinity of the exportin-1-mediated mRNA export machinery where it has been posited to act as an adaptor (PMID:34604117). Beyond this single co-purification with GBP2, no direct functional experiments have been performed on PBANKA_0506100 itself, and the corpus establishes nothing about this protein beyond its membership in this interaction (PMID:34604117).

1. 2021 Low

   To begin mapping the Plasmodium mRNA export machinery, it was unknown which proteins associate with GBP2; IP-MS revealed PBANKA_0506100 as a GBP2 interaction partner, placing this PHAX domain-containing protein within the exportin-1 export pathway.

   Evidence Immunoprecipitation coupled to mass spectrometry (IP-MS) in P. berghei

   PMID:34604117

   Open questions at the time

   • Single Co-IP/MS without reciprocal pull-down or direct validation of the interaction from PBANKA_0506100's perspective
   • No functional assay (knockout, knockdown, or complementation) on PBANKA_0506100 itself
   • The proposed adaptor role for exportin-1-mediated mRNA export is inferred from GBP2 context, not demonstrated for PBANKA_0506100

• Whether PBANKA_0506100 has any direct, autonomous function in mRNA export or another process remains unestablished.
• No loss-of-function phenotype for PBANKA_0506100
• No confirmed molecular activity, substrate, or localization for the protein itself
• No structural or domain-function data beyond annotation as PHAX domain-containing