{"gene":"PBANKA_0506100","run_date":"2026-09-07T10:55:50","timeline":{"discoveries":[{"year":2021,"finding":"The PHAX domain-containing protein (PBANKA_0506100) was identified as an interaction partner of GBP2 by immunoprecipitation coupled to mass spectrometry (IP-MS), implicating it as an adaptor protein for exportin-1 in the mRNA export pathway of malaria parasites.","method":"Immunoprecipitation coupled to mass spectrometry (IP-MS)","journal":"Frontiers in cellular and infection microbiology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single Co-IP/MS experiment identifying an interaction, single lab, no direct functional validation of PBANKA_0506100 itself","pmids":["34604117"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"PBANKA_0506100 (PHAX domain-containing protein) has been identified as an interaction partner of GBP2 in Plasmodium berghei by IP-MS, suggesting it may serve as an adaptor for exportin-1-mediated mRNA export, but no direct functional experiments have been performed on this protein itself."},"narrative":{"mechanistic_narrative":"PBANKA_0506100 is a PHAX domain-containing protein of Plasmodium berghei identified as an interaction partner of GBP2 by IP-MS, placing it in the vicinity of the exportin-1-mediated mRNA export machinery where it has been posited to act as an adaptor [PMID:34604117]. Beyond this single co-purification with GBP2, no direct functional experiments have been performed on PBANKA_0506100 itself, and the corpus establishes nothing about this protein beyond its membership in this interaction [PMID:34604117].","teleology":[{"year":2021,"claim":"To begin mapping the Plasmodium mRNA export machinery, it was unknown which proteins associate with GBP2; IP-MS revealed PBANKA_0506100 as a GBP2 interaction partner, placing this PHAX domain-containing protein within the exportin-1 export pathway.","evidence":"Immunoprecipitation coupled to mass spectrometry (IP-MS) in P. berghei","pmids":["34604117"],"confidence":"Low","gaps":["Single Co-IP/MS without reciprocal pull-down or direct validation of the interaction from PBANKA_0506100's perspective","No functional assay (knockout, knockdown, or complementation) on PBANKA_0506100 itself","The proposed adaptor role for exportin-1-mediated mRNA export is inferred from GBP2 context, not demonstrated for PBANKA_0506100"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"Whether PBANKA_0506100 has any direct, autonomous function in mRNA export or another process remains unestablished.","evidence":"No direct functional characterization of PBANKA_0506100 exists in the corpus","pmids":[],"confidence":"Low","gaps":["No loss-of-function phenotype for PBANKA_0506100","No confirmed molecular activity, substrate, or localization for the protein itself","No structural or domain-function data beyond annotation as PHAX domain-containing"],"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["GBP2"],"other_free_text":["Identified only as a GBP2 interaction partner by IP-MS in P. berghei; posited to act as an adaptor in exportin-1-mediated mRNA export, but no direct functional evidence exists for PBANKA_0506100 itself [#0]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"34604117","id":"PMC_34604117","title":"Roles and Cellular Localization of GBP2 and NAB2 During the Blood Stage of Malaria Parasites.","date":"2021","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/34604117","citation_count":6,"is_preprint":false,"source_species":["Pb"],"role":"primary","match_terms":["PHAX"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3478,"output_tokens":342,"usd":0.007782,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4779,"output_tokens":995,"usd":0.024385,"stage2_stop_reason":"end_turn"},"total_usd":0.032167,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2021,\n      \"finding\": \"The PHAX domain-containing protein (PBANKA_0506100) was identified as an interaction partner of GBP2 by immunoprecipitation coupled to mass spectrometry (IP-MS), implicating it as an adaptor protein for exportin-1 in the mRNA export pathway of malaria parasites.\",\n      \"method\": \"Immunoprecipitation coupled to mass spectrometry (IP-MS)\",\n      \"journal\": \"Frontiers in cellular and infection microbiology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single Co-IP/MS experiment identifying an interaction, single lab, no direct functional validation of PBANKA_0506100 itself\",\n      \"pmids\": [\"34604117\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PBANKA_0506100 (PHAX domain-containing protein) has been identified as an interaction partner of GBP2 in Plasmodium berghei by IP-MS, suggesting it may serve as an adaptor for exportin-1-mediated mRNA export, but no direct functional experiments have been performed on this protein itself.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PBANKA_0506100 is a PHAX domain-containing protein of Plasmodium berghei identified as an interaction partner of GBP2 by IP-MS, placing it in the vicinity of the exportin-1-mediated mRNA export machinery where it has been posited to act as an adaptor [#0]. Beyond this single co-purification with GBP2, no direct functional experiments have been performed on PBANKA_0506100 itself, and the corpus establishes nothing about this protein beyond its membership in this interaction [#0].\",\n  \"teleology\": [\n    {\n      \"year\": 2021,\n      \"claim\": \"To begin mapping the Plasmodium mRNA export machinery, it was unknown which proteins associate with GBP2; IP-MS revealed PBANKA_0506100 as a GBP2 interaction partner, placing this PHAX domain-containing protein within the exportin-1 export pathway.\",\n      \"evidence\": \"Immunoprecipitation coupled to mass spectrometry (IP-MS) in P. berghei\",\n      \"pmids\": [\"34604117\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single Co-IP/MS without reciprocal pull-down or direct validation of the interaction from PBANKA_0506100's perspective\",\n        \"No functional assay (knockout, knockdown, or complementation) on PBANKA_0506100 itself\",\n        \"The proposed adaptor role for exportin-1-mediated mRNA export is inferred from GBP2 context, not demonstrated for PBANKA_0506100\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether PBANKA_0506100 has any direct, autonomous function in mRNA export or another process remains unestablished.\",\n      \"evidence\": \"No direct functional characterization of PBANKA_0506100 exists in the corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No loss-of-function phenotype for PBANKA_0506100\",\n        \"No confirmed molecular activity, substrate, or localization for the protein itself\",\n        \"No structural or domain-function data beyond annotation as PHAX domain-containing\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\"GBP2\"],\n    \"other_free_text\": [\n      \"Identified only as a GBP2 interaction partner by IP-MS in P. berghei; posited to act as an adaptor in exportin-1-mediated mRNA export, but no direct functional evidence exists for PBANKA_0506100 itself [#0].\"\n    ]\n  }\n}","audit_flag":{"gene":"PBANKA_0506100","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 1 of 1 cited papers name other catalogue genes (PF3D7_0320800, PF3D7_0623100, PF3D7_1006800). The summary may describe one of those genes."},"strains":[{"strain":"PbANKA","gene_id":"PBANKA_0506100","name":null,"product":"PHAX domain-containing protein, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"","evaluation":null}