EF-1gamma

EF-1gamma (PF3D7_1338300) is a putative subunit of the Plasmodium falciparum translation elongation factor complex, identified by tandem affinity purification in which it co-purified under native conditions with tagged PfEF-1beta together with native PfEF-1beta, PfEF-1alpha, and PfEF-1delta (PMID:17307963). Beyond this membership in the elongation factor complex, no function specific to EF-1gamma itself has been characterized in the available corpus; the affinity purification defines it as a native interaction partner but does not resolve its individual biochemical role.

1. 2007 Medium

   Whether P. falciparum assembles a multi-subunit elongation factor complex and which proteins compose it was unknown; tandem affinity purification established that EF-1gamma physically associates with PfEF-1beta, PfEF-1alpha, and PfEF-1delta as part of a native complex.

   Evidence Tandem affinity purification of PfEF-1beta-PTP from crude P. falciparum extracts with mass spectrometric identification of co-purified proteins

   PMID:17307963

   Open questions at the time

   • Single-direction affinity purification without reciprocal pulldown using EF-1gamma as bait
   • No functional validation of EF-1gamma's specific role within the complex
   • Stoichiometry and direct binding partner of EF-1gamma within the complex not resolved

• The specific biochemical activity, essentiality, and mechanistic contribution of EF-1gamma to elongation within the complex remain undefined.
• No enzymatic or biochemical activity attributed to EF-1gamma specifically
• No genetic or functional perturbation data for EF-1gamma
• No structural model of the complex or of EF-1gamma's position within it