{"gene":"PF3D7_1338300","run_date":"2026-09-08T14:19:06","timeline":{"discoveries":[{"year":2007,"finding":"EF-1gamma (PF3D7_1338300) was identified as a component of the P. falciparum translation elongation factor complex, co-purifying with PfEF-1beta (tagged bait), native PfEF-1beta, PfEF-1alpha, and PfEF-1delta under native conditions.","method":"Tandem affinity purification (TAP) of PfEF-1beta-PTP from crude P. falciparum extracts followed by mass spectrometric identification of co-purified proteins","journal":"Eukaryotic cell","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — single-direction affinity purification with MS identification in one lab; no reciprocal pulldown or functional validation of EF-1gamma specifically","pmids":["17307963"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"EF-1gamma (PF3D7_1338300) is a subunit of the P. falciparum translation elongation factor complex, identified by tandem affinity purification as a native interaction partner of PfEF-1beta, PfEF-1alpha, and PfEF-1delta; no further mechanistic details about its specific function within the complex have been established."},"narrative":{"mechanistic_narrative":"EF-1gamma (PF3D7_1338300) is a putative subunit of the Plasmodium falciparum translation elongation factor complex, identified by tandem affinity purification in which it co-purified under native conditions with tagged PfEF-1beta together with native PfEF-1beta, PfEF-1alpha, and PfEF-1delta [PMID:17307963]. Beyond this membership in the elongation factor complex, no function specific to EF-1gamma itself has been characterized in the available corpus; the affinity purification defines it as a native interaction partner but does not resolve its individual biochemical role.","teleology":[{"year":2007,"claim":"Whether P. falciparum assembles a multi-subunit elongation factor complex and which proteins compose it was unknown; tandem affinity purification established that EF-1gamma physically associates with PfEF-1beta, PfEF-1alpha, and PfEF-1delta as part of a native complex.","evidence":"Tandem affinity purification of PfEF-1beta-PTP from crude P. falciparum extracts with mass spectrometric identification of co-purified proteins","pmids":["17307963"],"confidence":"Medium","gaps":["Single-direction affinity purification without reciprocal pulldown using EF-1gamma as bait","No functional validation of EF-1gamma's specific role within the complex","Stoichiometry and direct binding partner of EF-1gamma within the complex not resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The specific biochemical activity, essentiality, and mechanistic contribution of EF-1gamma to elongation within the complex remain undefined.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No enzymatic or biochemical activity attributed to EF-1gamma specifically","No genetic or functional perturbation data for EF-1gamma","No structural model of the complex or of EF-1gamma's position within it"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["P. falciparum translation elongation factor complex (with PfEF-1beta, PfEF-1alpha, PfEF-1delta)"],"partners":["PFEF-1BETA","PFEF-1ALPHA","PFEF-1DELTA"],"other_free_text":["Identified only as a native co-purifying component of the elongation factor complex; no EF-1gamma-specific function established in the available corpus."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"17307963","id":"PMC_17307963","title":"Purification of components of the translation elongation factor complex of Plasmodium falciparum by tandem affinity purification.","date":"2007","source":"Eukaryotic cell","url":"https://pubmed.ncbi.nlm.nih.gov/17307963","citation_count":15,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3407,"output_tokens":391,"usd":0.008043,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4864,"output_tokens":1047,"usd":0.025247,"stage2_stop_reason":"end_turn"},"total_usd":0.03329,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2007,\n      \"finding\": \"EF-1gamma (PF3D7_1338300) was identified as a component of the P. falciparum translation elongation factor complex, co-purifying with PfEF-1beta (tagged bait), native PfEF-1beta, PfEF-1alpha, and PfEF-1delta under native conditions.\",\n      \"method\": \"Tandem affinity purification (TAP) of PfEF-1beta-PTP from crude P. falciparum extracts followed by mass spectrometric identification of co-purified proteins\",\n      \"journal\": \"Eukaryotic cell\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — single-direction affinity purification with MS identification in one lab; no reciprocal pulldown or functional validation of EF-1gamma specifically\",\n      \"pmids\": [\"17307963\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"EF-1gamma (PF3D7_1338300) is a subunit of the P. falciparum translation elongation factor complex, identified by tandem affinity purification as a native interaction partner of PfEF-1beta, PfEF-1alpha, and PfEF-1delta; no further mechanistic details about its specific function within the complex have been established.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"EF-1gamma (PF3D7_1338300) is a putative subunit of the Plasmodium falciparum translation elongation factor complex, identified by tandem affinity purification in which it co-purified under native conditions with tagged PfEF-1beta together with native PfEF-1beta, PfEF-1alpha, and PfEF-1delta [#0]. Beyond this membership in the elongation factor complex, no function specific to EF-1gamma itself has been characterized in the available corpus; the affinity purification defines it as a native interaction partner but does not resolve its individual biochemical role.\",\n  \"teleology\": [\n    {\n      \"year\": 2007,\n      \"claim\": \"Whether P. falciparum assembles a multi-subunit elongation factor complex and which proteins compose it was unknown; tandem affinity purification established that EF-1gamma physically associates with PfEF-1beta, PfEF-1alpha, and PfEF-1delta as part of a native complex.\",\n      \"evidence\": \"Tandem affinity purification of PfEF-1beta-PTP from crude P. falciparum extracts with mass spectrometric identification of co-purified proteins\",\n      \"pmids\": [\"17307963\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Single-direction affinity purification without reciprocal pulldown using EF-1gamma as bait\",\n        \"No functional validation of EF-1gamma's specific role within the complex\",\n        \"Stoichiometry and direct binding partner of EF-1gamma within the complex not resolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific biochemical activity, essentiality, and mechanistic contribution of EF-1gamma to elongation within the complex remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No enzymatic or biochemical activity attributed to EF-1gamma specifically\",\n        \"No genetic or functional perturbation data for EF-1gamma\",\n        \"No structural model of the complex or of EF-1gamma's position within it\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006414\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"complexes\": [\"P. falciparum translation elongation factor complex (with PfEF-1beta, PfEF-1alpha, PfEF-1delta)\"],\n    \"partners\": [\"PfEF-1beta\", \"PfEF-1alpha\", \"PfEF-1delta\"],\n    \"other_free_text\": [\n      \"Identified only as a native co-purifying component of the elongation factor complex; no EF-1gamma-specific function established in the available corpus.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1338300","name":null,"product":"elongation factor 1-gamma, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1352000","name":null,"product":"elongation factor 1-gamma, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1263000","name":null,"product":"elongation factor 1-gamma, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"EF-1gamma","evaluation":null}