BUD32

In P. falciparum, BUD32 (PF3D7_0708300) is a component of the conserved EKC/KEOPS complex, interacting with the cytoplasmic proteins Kae1 and Cgi121 to recapitulate the complex composition seen in other organisms (PMID:25204654). Beyond this membership in a cytoplasmic Kae1/Cgi121-containing complex established by immunoprecipitation-mass spectrometry (PMID:25204654), no enzymatic activity, structure, or additional mechanistic detail has been characterized for BUD32 itself in the available corpus.

1. 2014 Medium

   Whether P. falciparum possesses an EKC/KEOPS complex and which proteins compose it was unknown; identifying BUD32 in a cytoplasmic complex with Kae1 and Cgi121 established that the conserved complex composition is preserved in this parasite.

   Evidence Immunoprecipitation-mass spectrometry with cross-compartment proteomic cross-validation in P. falciparum

   PMID:25204654

   Open questions at the time

   • Interaction detected by IP-MS but not confirmed by reconstitution or reciprocal pulldown
   • No enzymatic activity or catalytic contribution of BUD32 was tested
   • No mutagenesis was performed to map the interaction interfaces

• The molecular activity, structure, and cellular function of BUD32 beyond its physical membership in the EKC/KEOPS complex remain undetermined.
• No biochemical activity assigned to BUD32 itself
• No structural model of the P. falciparum complex or BUD32 subunit
• No genetic or phenotypic characterization of BUD32 function in the parasite