PF3D7_1139700

PF3D7_1139700 is annotated as a putative adrenodoxin reductase and characterized as a ferredoxin-NADP(+) reductase (FNR) of the glutathione reductase structural type in Plasmodium falciparum, with biochemical evidence indicating novel modes of FAD- and NADP(H)-binding relative to other FNR family members (PMID:18307973). Within this family, adrenodoxin reductase homologs can catalyze oxidation of NADP(+) to its 4-oxo derivative (PMID:18307973). Beyond this single biochemical characterization (PMID:18307973), no further mechanistic, structural, or in vivo functional detail specific to PF3D7_1139700 has been characterized in the available corpus.

1. 2008 Low

   Establishing whether the Plasmodium falciparum FNR/adrenodoxin reductase homolog behaves like canonical FNR enzymes addressed whether this parasite protein could be a distinct, potentially druggable flavoenzyme.

   Evidence Biochemical characterization and structural comparison of P. falciparum FNR within the FNR family

   PMID:18307973

   Open questions at the time

   • No primary in vitro kinetic assay or mutagenesis data on PF3D7_1139700 itself is described
   • No structural model of the parasite protein is provided
   • Physiological electron partner (ferredoxin vs adrenodoxin) and cellular role in P. falciparum are not established

• It remains unknown what the endogenous redox partners, subcellular localization, and biological pathway of PF3D7_1139700 are, and whether it is essential or validated as a drug target in P. falciparum.
• No genetic knockout or essentiality data
• No reconstitution of electron transfer with a defined parasite ferredoxin/adrenodoxin
• No structural determination of the parasite enzyme