RAD23

PF3D7_1011700 (RAD23) is a putative P. falciparum DNA repair protein placed within the parasite's excision repair response to genotoxic stress: in the mutator strains Dd2 and W2, which fail to mount a DNA repair transcriptional response after MMS-induced damage, RAD23 was one of 18 DNA repair genes found to carry point mutations (PMID:27033103). It has additionally been named as a component of the parasite ubiquitin-proteasome system alongside Dsk2 and Ddi1, linked to the unfolded protein response under stress (PMID:40113723). No direct biochemical, structural, or functional experiment has been performed on PF3D7_1011700 itself in the available corpus; both findings mention it as a member of a gene set or complex rather than characterizing its mechanism, so beyond these associations its molecular activity, partners, and role are uncharacterized.

1. 2016 Low

   To locate RAD23 within the parasite's genotoxic stress response, transcriptional and genomic profiling of MMS-treated mutator strains tested which DNA repair genes are compromised when repair induction fails.

   Evidence Genome-wide transcriptional profiling after MMS treatment plus genome sequencing of Dd2 and W2 mutator strains

   PMID:27033103

   Open questions at the time

   • RAD23 is one of 18 listed genes with no individual functional assay specific to it
   • point mutations were not shown to alter RAD23 function or repair activity
   • no direct evidence that RAD23 participates in excision repair mechanistically
2. 2025 Low

   To situate RAD23 in the parasite proteostasis network, a study of PfDdi1 named RAD23 as a co-member of the ubiquitin-proteasome system tied to the unfolded protein response.

   Evidence Computational docking and molecular dynamics simulation focused on PfDdi1, with RAD23 cited contextually as a UPS component alongside Dsk2 and Ddi1

   PMID:40113723

   Open questions at the time

   • no experiment was performed on RAD23 itself in this study
   • physical interaction of RAD23 with Dsk2, Ddi1, or the proteasome not demonstrated
   • role of RAD23 in the unfolded protein response not directly tested

• Whether PF3D7_1011700 has any direct biochemical activity, binding partners, or defined role in either DNA repair or the ubiquitin-proteasome system remains entirely uncharacterized.
• no direct enzymatic or binding assay on RAD23
• no structural model specific to RAD23
• no genetic knockout or complementation defining its cellular role