SRCAP

PF3D7_0820000 (SRCAP) is established in the available corpus only as a subunit of the PfSWR1 chromatin-remodeling complex in P. falciparum, which associates with the variant histones H2A.Z and H2B.Z and is implicated in their deposition (PMID:38564734). Its membership was defined by reciprocal affinity pulldowns of the complex rather than by any experiment on PF3D7_0820000 itself, so no direct enzymatic, remodeling, or functional activity has been demonstrated for the protein (PMID:38564734). Beyond this complex membership and a low-confidence yeast-two-hybrid interaction with PfCDPK6 (PMID:24267797), no mechanistic detail specific to PF3D7_0820000 has been characterized in the available corpus.

1. 2013 Low

   The first question was whether PF3D7_0820000 participates in any protein interaction network; a Y2H screen placed it as an interaction partner of PfCDPK6 and computationally linked it to base excision repair and chromosome remodeling, providing an initial but indirect hint of chromatin-related function.

   Evidence Yeast two-hybrid interaction screen with computational subnetwork alignment in P. falciparum

   PMID:24267797

   Open questions at the time

   • Y2H interaction with PfCDPK6 not independently confirmed and no direct functional assay on PF3D7_0820000
   • chromatin-remodeling role only computationally predicted, not experimentally tested
   • no localization or expression data for the protein
2. 2024 Medium

   The question of which complex PF3D7_0820000 belongs to was answered by defining it as a subunit of the PfSWR1 chromatin-remodeling complex, establishing that the complex it resides in associates with and deposits the variant histones H2A.Z and H2B.Z.

   Evidence Reciprocal affinity pulldown (PfEAF2::GFP GFP-trap) with mass spectrometry and SAINT analysis in P. falciparum

   PMID:38564734

   Open questions at the time

   • membership established but no direct functional assay performed on PF3D7_0820000 itself
   • specific contribution of PF3D7_0820000 to H2A.Z/H2B.Z deposition not resolved
   • no enzymatic, structural, or localization data specific to the subunit

• It remains unknown what direct molecular activity PF3D7_0820000 contributes to the PfSWR1 complex and whether it is individually required for H2A.Z/H2B.Z deposition or parasite viability.
• no genetic knockout or knockdown of PF3D7_0820000 reported
• no reconstitution or biochemical activity assay for the subunit
• no structural model of PF3D7_0820000 within PfSWR1