Affinage · Plasmodium

PF3D7_0727400

proteasome subunit alpha type-5, putative

Annotated
2026-09-08
7 papers in source corpus 1 papers cited in narrative 1 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PF3D7_0727400 (proteasome subunit alpha type-5, putative) is a structural component of the Plasmodium falciparum 20S proteasome (Pf20S), whose overall architecture was resolved by cryo-EM at 3.6 Å (PMID:26863983). Beyond this membership in the Pf20S complex, no subunit-specific mechanistic role, binding partner, post-translational modification, or enzymatic activity has been characterized for PF3D7_0727400 individually in the available corpus (PMID:26863983).

Mechanistic history

Synthesis pass · year-by-year structured walk · 1 step
  1. 2016 Low

    Establishing the subunit composition and architecture of the P. falciparum 20S proteasome placed PF3D7_0727400 within the assembled Pf20S complex as an alpha-type structural subunit.

    Evidence Cryo-electron microscopy and single-particle analysis of the intact Pf20S complex at 3.6 Å

    PMID:26863983

    Open questions at the time
    • Alpha subunit membership is inferred from the overall complex structure; no subunit-specific functional or mechanistic data were reported for PF3D7_0727400
    • No binding partner, post-translational modification, or catalytic contribution has been assigned to this individual subunit
    • The specific role of PF3D7_0727400 in proteasome assembly, substrate gating, or stability is undetermined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Whether PF3D7_0727400 has any subunit-specific function distinct from its structural role in the Pf20S complex remains unknown.
  • No experimental data isolate the contribution of PF3D7_0727400 from the intact complex
  • No essentiality, knockdown, or mutational analysis of this subunit is available
  • No interaction partners outside the core proteasome have been identified

Mechanism profile

Synthesis pass · controlled-vocabulary classification
Complex memberships
P. falciparum 20S proteasome (Pf20S) [#0]

Evidence

Reading pass · 1 per-paper finding extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2016 PF3D7_0727400 (proteasome subunit alpha type-5) is a structural component of the P. falciparum 20S proteasome (Pf20S), whose overall architecture was resolved by cryo-EM at 3.6 Å; however, no specific mechanistic finding was reported for this alpha subunit individually. Cryo-electron microscopy and single-particle analysis of the intact Pf20S complex Nature Low 26863983

Source papers

Stage 0 corpus · 7 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2016 Structure- and function-based design of Plasmodium-selective proteasome inhibitors. Nature 196 26863983
2012 The proteasome of malaria parasites: A multi-stage drug target for chemotherapeutic intervention? International journal for parasitology. Drugs and drug resistance 64 24533266
2019 The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis. Nature microbiology 37 31384003
2021 Design of proteasome inhibitors with oral efficacy in vivo against Plasmodium falciparum and selectivity over the human proteasome. Proceedings of the National Academy of Sciences of the United States of America 33 34548400
2023 Structures revealing mechanisms of resistance and collateral sensitivity of Plasmodium falciparum to proteasome inhibitors. Nature communications 21 38097652
2024 Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria. Cell chemical biology 11 39084225
2025 Optimization of Species-Selective Reversible Proteasome Inhibitors for the Treatment of Malaria. Journal of medicinal chemistry 1 41148577