CRISP

PF3D7_0705700 (S29) is a structural constituent of the 40S small subunit of the Plasmodium falciparum 80S ribosome, placed in its structural context by cryo-EM of the intact ribosome at 3.2 Å resolution (PMID:24913268) and by multiple reconstructions of ribosomes purified from blood-stage schizonts that resolved distinct translational states and features distinguishing the parasite ribosome from mammalian ribosomes (PMID:26432834). In situ cryo-electron tomography of the native ribosome inside infected erythrocytes has further situated the 40S subunit, and thereby S29, within actively translating states of the elongation cycle (PMID:40825879). Beyond its assignment as a small-subunit component in these structures, no experiment in the available corpus has directly interrogated the individual function, binding partners, or post-translational modifications of PF3D7_0705700; the corpus establishes only its membership in the 40S small subunit.

1. 2014 Medium

   The first atomic-resolution structure of the P. falciparum 80S ribosome was needed to place small-subunit proteins in structural context and to understand small-molecule inhibition; this established S29 as a resolved constituent of the 40S small subunit.

   Evidence Cryo-EM at 3.2 Å of the P. falciparum 80S ribosome bound to emetine

   PMID:24913268

   Open questions at the time

   • No functional experiment targeted S29 individually
   • Binding partners of S29 beyond the ribosome not defined
   • No post-translational modification data
2. 2015 Medium

   To capture the parasite ribosome across functional states, multiple reconstructions from schizont-purified ribosomes resolved S29 as a small-subunit component in several translational conformations and identified features distinguishing the parasite from the mammalian ribosome.

   Evidence Cryo-EM with multiple reconstructions and flexible-fitting atomic model building of ribosomes from P. falciparum schizonts

   PMID:26432834

   Open questions at the time

   • Structural placement only; no assay of S29-specific contribution to translation
   • Species-specific features not attributed to S29 in particular
3. 2025 Medium

   Whether the ribosome's structural arrangement seen in purified samples holds in the native cell was addressed by in situ tomography, situating the 40S subunit, and S29, within actively translating states inside infected erythrocytes.

   Evidence In situ cryo-electron tomography of P. falciparum-infected erythrocytes

   PMID:40825879

   Open questions at the time

   • S29 not individually interrogated
   • No mechanistic role assigned to S29 within elongation
   • No interaction or modification data

• The individual function, binding partners, post-translational modifications, and essentiality of PF3D7_0705700 beyond its structural membership in the 40S small subunit remain uncharacterized in the available corpus.
• No gene-specific knockdown, knockout, or complementation for S29
• No S29-specific interactome
• No evidence for extraribosomal or moonlighting roles