NUP434

NUP434 (PF3D7_0212500) is a component of the Plasmodium nuclear pore complex (NPC), identified by TurboID proximity labeling coupled to affinity purification and mass spectrometry and confirmed by GFP tagging, with failed gene deletion indicating it is essential for parasite survival (PMID:36040030). Beyond its assignment to the NPC and its essentiality, no further mechanistic detail—including its precise molecular function, its interactions with specific nucleoporins, or its structural role within the pore—has been characterized in the available corpus.

1. 2022 Medium

   Whether PF3D7_0212500 is a bona fide constituent of the parasite nuclear pore and whether it matters for viability was unknown; proximity labeling placed it at the NPC and deletion attempts established that it is required for survival.

   Evidence TurboID proximity labeling, affinity purification and mass spectrometry, GFP tagging, and gene deletion in Plasmodium berghei

   PMID:36040030

   Open questions at the time

   • The specific molecular function of NUP434 within the NPC is not defined
   • Direct binding partners or subcomplex membership among nucleoporins are not resolved
   • Essentiality is inferred from failed deletion rather than a conditional knockdown demonstrating the terminal phenotype

• The precise molecular role of NUP434 within the nuclear pore—its structural position, direct interactors, and the cellular process it enables—remains to be determined.
• No mechanistic dissection of NUP434 function beyond NPC association
• No structural model or defined subcomplex placement
• No conditional depletion linking NUP434 loss to a specific defect