{"gene":"PKNH_0104800","run_date":"2026-09-11T10:06:41","timeline":{"discoveries":[{"year":2022,"finding":"PKNH_0104800 (AKIT11) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, placing it as a constituent of the apicomplexan kinetochore complex.","method":"Interactomics (co-immunoprecipitation/proximity-ligation-based proteomics) combined with deep homology searches","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — interactomics-based identification in a single study; complex membership established but no further functional dissection of PKNH_0104800 itself reported","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"PKNH_0104800 (AKIT11) is a component of the apicomplexan kinetochore complex, identified by interactomics and deep homology searches as one of 13 newly discovered kinetochore proteins in Apicomplexa; no further mechanistic details about its specific function within the kinetochore are established in the available literature."},"narrative":{"mechanistic_narrative":"PKNH_0104800 (AKIT11) is a constituent of the apicomplexan kinetochore complex, identified in Plasmodium berghei as one of 13 previously unknown kinetochore components through interactomics combined with deep homology searches [PMID:36006241]. Beyond this complex membership, no mechanistic detail about the specific function of PKNH_0104800 within the kinetochore has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"The composition of the apicomplexan kinetochore was largely undefined; identifying its constituents established which proteins assemble at this structure, and placed PKNH_0104800 among them.","evidence":"Interactomics (co-immunoprecipitation/proximity-ligation-based proteomics) combined with deep homology searches in Plasmodium knowlesi","pmids":["36006241"],"confidence":"Medium","gaps":["No functional dissection of PKNH_0104800 itself within the kinetochore","No direct binding partners or substructural position of PKNH_0104800 defined","Complex membership rests on a single interactomics study without independent validation"],"attribution":[{"species":"Plasmodium knowlesi","strain":""}]},{"year":null,"claim":"The specific molecular role of PKNH_0104800 within the kinetochore, its direct interaction partners, and its contribution to chromosome segregation remain unknown.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No knockout/knockdown phenotype for PKNH_0104800 reported","No structural or biochemical characterization of PKNH_0104800","No demonstrated role in kinetochore-microtubule attachment or mitotic progression"],"attribution":[{"species":"Plasmodium knowlesi","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["apicomplexan kinetochore complex"],"partners":[],"other_free_text":["Identified as one of 13 previously unknown apicomplexan kinetochore components in Plasmodium knowlesi via interactomics and deep homology searches [#0]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":31,"is_preprint":false,"source_species":["Pk"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3241,"output_tokens":354,"usd":0.007516,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4814,"output_tokens":879,"usd":0.023023,"stage2_stop_reason":"end_turn"},"total_usd":0.030539,"stage1_batch_id":"msgbatch_01S4WrhSkksYHeZXxqf3za3o","stage2_batch_id":"msgbatch_019VBBeuW6G52X8gZfsFDfb5","note":"batch pricing = 50% of standard","patch_2026_09_11":["species attribution corrected after review"]},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"PKNH_0104800 (AKIT11) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, placing it as a constituent of the apicomplexan kinetochore complex.\",\n      \"method\": \"Interactomics (co-immunoprecipitation/proximity-ligation-based proteomics) combined with deep homology searches\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — interactomics-based identification in a single study; complex membership established but no further functional dissection of PKNH_0104800 itself reported\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium knowlesi\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PKNH_0104800 (AKIT11) is a component of the apicomplexan kinetochore complex, identified by interactomics and deep homology searches as one of 13 newly discovered kinetochore proteins in Apicomplexa; no further mechanistic details about its specific function within the kinetochore are established in the available literature.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PKNH_0104800 (AKIT11) is a constituent of the apicomplexan kinetochore complex, identified in Plasmodium knowlesi as one of 13 previously unknown kinetochore components through interactomics combined with deep homology searches [#0]. Beyond this complex membership, no mechanistic detail about the specific function of PKNH_0104800 within the kinetochore has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"The composition of the apicomplexan kinetochore was largely undefined; identifying its constituents established which proteins assemble at this structure, and placed PKNH_0104800 among them.\",\n      \"evidence\": \"Interactomics (co-immunoprecipitation/proximity-ligation-based proteomics) combined with deep homology searches in Plasmodium knowlesi\",\n      \"pmids\": [\n        \"36006241\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional dissection of PKNH_0104800 itself within the kinetochore\",\n        \"No direct binding partners or substructural position of PKNH_0104800 defined\",\n        \"Complex membership rests on a single interactomics study without independent validation\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium knowlesi\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular role of PKNH_0104800 within the kinetochore, its direct interaction partners, and its contribution to chromosome segregation remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No knockout/knockdown phenotype for PKNH_0104800 reported\",\n        \"No structural or biochemical characterization of PKNH_0104800\",\n        \"No demonstrated role in kinetochore-microtubule attachment or mitotic progression\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium knowlesi\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [\n      \"apicomplexan kinetochore complex\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as one of 13 previously unknown apicomplexan kinetochore components in Plasmodium knowlesi via interactomics and deep homology searches [#0]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"PkH","gene_id":"PKNH_0104800","name":null,"product":"apicomplexan kinetochore protein 11, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AKIT11","evaluation":null}