{"gene":"PF3D7_1468900","run_date":"2026-09-08T14:19:07","timeline":{"discoveries":[{"year":2022,"finding":"The P. falciparum CCCH zinc finger protein ZNF4 (a candidate for PF3D7_1468900 based on corpus ranking) is highly expressed in mature gametocytes and predominantly localizes to the cytoplasm. Targeted gene disruption of ZNF4 showed no significant effect on asexual blood stage replication or gametocyte development, but male gametocyte exflagellation was significantly impaired, leading to reduced malaria transmission to mosquitoes.","method":"Targeted gene disruption (knockout), fluorescence localization, transmission experiments in mosquitoes","journal":"Cells","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean KO with defined cellular phenotype (exflagellation defect), localization by imaging, mosquito transmission assay; single lab","pmids":["35626703"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"finding":"Comparative transcriptomics between wildtype and ZNF4-KO parasites revealed deregulation of ~473 genes in mature gametocytes, with downregulated genes being male-enriched and associated with axonemal dynein complex formation and cell projection organization, indicating ZNF4 regulates male gametocyte-enriched transcripts post-transcriptionally.","method":"Comparative transcriptomics (RNA-seq) of KO vs wildtype gametocytes","journal":"Cells","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — transcriptomic analysis in KO parasites with defined phenotype; single lab, single study","pmids":["35626703"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"finding":"ZNF4 was identified as a protein interactor of MD3 (another CCCH zinc finger protein) in P. falciparum gametocytes, detected by BioID analysis and co-immunoprecipitation, placing ZNF4 within a regulator complex involved in post-transcriptional fine-tuning of male gametocytogenesis.","method":"BioID proximity labeling and co-immunoprecipitation","journal":"Molecular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal Co-IP and BioID in a single study; ZNF4 is listed as an interactor of MD3, not the primary subject","pmids":["38148574"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"finding":"A global barcoded mutant screen in P. berghei identified a female-determining zinc-finger protein whose protein interactors indicate participation in germ-granule-like ribonucleoprotein complexes, complementing transcriptional processes in female sexual development and transmission to mosquitoes.","method":"Global screen of barcoded mutants, single-cell transcriptomics of mutant parasites","journal":"Cell host & microbe","confidence":"Low","confidence_rationale":"Tier 3 / Weak — screen-level identification without detailed mechanistic follow-up for this specific protein; identity with PF3D7_1468900 not explicitly confirmed in abstract","pmids":["36634679"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"PF3D7_1468900 (putative zinc finger protein, likely ZNF4 based on corpus evidence) is a CCCH-type zinc finger protein expressed in mature P. falciparum gametocytes that localizes to the cytoplasm, is required for male gametocyte exflagellation and mosquito transmission by regulating male-enriched transcripts (including axonemal dynein complex genes), and participates in a multi-protein ribonucleoprotein regulatory complex in gametocytes that includes MD3, PABP1, and other RNA-binding proteins."},"narrative":{"mechanistic_narrative":"PF3D7_1468900 is a putative CCCH-type zinc finger protein (corpus candidate ZNF4) that acts as a post-transcriptional regulator of male gametocyte maturation in Plasmodium falciparum, and is required for parasite transmission to the mosquito [PMID:35626703]. It is highly expressed in mature gametocytes and localizes predominantly to the cytoplasm; its targeted disruption does not affect asexual replication or gametocyte development but severely impairs male gametocyte exflagellation, reducing mosquito transmission [PMID:35626703]. Consistent with a post-transcriptional role, loss of the protein deregulates ~473 transcripts in mature gametocytes, with the downregulated set being male-enriched and functionally associated with axonemal dynein complex formation and cell-projection organization—transcripts underlying the flagellar machinery of exflagellation [PMID:35626703]. It functions within a gametocyte regulatory ribonucleoprotein complex, having been identified as an interactor of the CCCH zinc finger protein MD3 by BioID and co-immunoprecipitation [PMID:38148574]. Beyond this gametocyte RNA-regulatory role, no biochemical characterization of its RNA targets or zinc-finger binding activity has been established in the available corpus.","teleology":[{"year":2022,"claim":"Establishing whether this cytoplasmic gametocyte zinc finger protein has a stage-specific function, disruption revealed it is dispensable for asexual growth and gametocyte development but essential for male exflagellation and mosquito transmission, defining its role as a male-fertility/transmission factor.","evidence":"Targeted gene disruption with fluorescence localization and mosquito transmission assays in P. falciparum","pmids":["35626703"],"confidence":"Medium","gaps":["Definitive identity of PF3D7_1468900 as ZNF4 rests on corpus ranking rather than explicit confirmation","Mechanism linking cytoplasmic localization to exflagellation not resolved","Single-lab study without independent replication"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"To determine how the protein exerts its exflagellation function, transcriptomics of knockout gametocytes showed deregulation of ~473 genes with downregulated male-enriched axonemal dynein transcripts, establishing a post-transcriptional regulatory role over the flagellar transcriptome.","evidence":"Comparative RNA-seq of knockout versus wildtype mature gametocytes in P. falciparum","pmids":["35626703"],"confidence":"Medium","gaps":["Direct RNA targets bound by the protein not identified","Whether regulation is via mRNA stability, translation, or storage is unknown","No demonstration of direct zinc-finger RNA binding"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"claim":"To place the protein in its molecular environment, it was identified as an interactor of the CCCH zinc finger protein MD3, situating it within a multi-protein ribonucleoprotein complex fine-tuning male gametocytogenesis.","evidence":"BioID proximity labeling and co-immunoprecipitation in P. falciparum gametocytes (protein identified as an MD3 interactor, not the primary subject)","pmids":["38148574"],"confidence":"Medium","gaps":["Stoichiometry and full composition of the complex relative to this protein not defined","Whether the interaction with MD3 is direct is not established","The protein is a secondary hit in a study centered on MD3"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"claim":"A rodent-malaria screen linked a zinc-finger protein to female sexual development within germ-granule-like ribonucleoprotein complexes, raising the question of whether analogous complexes operate across sexes, though its identity with PF3D7_1468900 is not confirmed.","evidence":"Global barcoded mutant screen and single-cell transcriptomics in P. berghei","pmids":["36634679"],"confidence":"Low","gaps":["Identity of the P. berghei protein with PF3D7_1468900 is not explicitly confirmed","Screen-level identification without mechanistic follow-up","Sex assignment (female) differs from the male-specific P. falciparum phenotype, so orthology and function are unresolved"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The direct RNA targets, the RNA-binding activity of its CCCH zinc fingers, and the biochemical architecture of its ribonucleoprotein complex remain uncharacterized.","evidence":"","pmids":[],"confidence":"Low","gaps":["No CLIP or direct binding data identifying bound transcripts","No structural or domain-level analysis of the zinc fingers","Mechanism of post-transcriptional control (stability vs translation vs storage) unresolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["MD3-associated gametocyte ribonucleoprotein regulatory complex"],"partners":["MD3 (CCCH ZINC FINGER PROTEIN)"],"other_free_text":["Required for male gametocyte exflagellation and transmission to mosquitoes in P. falciparum [#0]","Dispensable for asexual blood-stage replication and gametocyte development [#0]","Loss deregulates ~473 transcripts in mature gametocytes, with downregulated male-enriched axonemal dynein genes [#1]","Identity of PF3D7_1468900 as ZNF4 is based on corpus ranking, not explicit confirmation","A P. berghei zinc finger protein linked to female sexual development in germ-granule-like RNP complexes may or may not correspond to this gene [#3]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36634679","id":"PMC_36634679","title":"Regulators of male and female sexual development are critical for the transmission of a malaria parasite.","date":"2023","source":"Cell host & microbe","url":"https://pubmed.ncbi.nlm.nih.gov/36634679","citation_count":53,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["zinc finger protein"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34418264","id":"PMC_34418264","title":"Zinc finger proteins of Plasmodium falciparum.","date":"2021","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/34418264","citation_count":33,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["zinc finger protein"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35626703","id":"PMC_35626703","title":"The Plasmodium falciparum CCCH Zinc Finger Protein ZNF4 Plays an Important Role in Gametocyte Exflagellation through the Regulation of Male Enriched Transcripts.","date":"2022","source":"Cells","url":"https://pubmed.ncbi.nlm.nih.gov/35626703","citation_count":20,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["zinc finger protein"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"27385072","id":"PMC_27385072","title":"An evolutionary conserved zinc finger protein is involved in Toxoplasma gondii mRNA nuclear export.","date":"2016","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/27385072","citation_count":14,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["zinc finger protein"],"competing_organism_flag":true,"competing_organism_match":"Toxoplasma","competing_organism_only":true},{"pmid":"38148574","id":"PMC_38148574","title":"The Plasmodium falciparum CCCH zinc finger protein MD3 regulates male gametocytogenesis through its interaction with RNA-binding proteins.","date":"2023","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/38148574","citation_count":8,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["zinc finger protein"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"28851851","id":"PMC_28851851","title":"Multifunctional Involvement of a C2H2 Zinc Finger Protein (PbZfp) in Malaria Transmission, Histone Modification, and Susceptibility to DNA Damage Response.","date":"2017","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/28851851","citation_count":5,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["zinc finger protein"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42244567","id":"PMC_42244567","title":"The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission.","date":"2026","source":"bioRxiv : the preprint server for biology","url":"https://pubmed.ncbi.nlm.nih.gov/42244567","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["zinc finger protein"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":12438,"output_tokens":2271,"usd":0.057258,"retried_sync":true},"stage2":{"model":"claude-opus-4-8","input_tokens":5941,"output_tokens":2544,"usd":0.046652,"stage2_stop_reason":"end_turn"},"total_usd":0.10391,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"Looking at the papers, I need to identify which ones are actually about PF3D7_1468900 (zinc finger protein, putative).\n\nPaper [1] mentions \"a female-determining zinc-finger protein\" whose protein interactors indicate germ-granule-like ribonucleoprotein complexes - this could be PF3D7_1468900, but it's not explicitly named.\n\nPaper [3] is about ZNF4 (a CCCH zinc finger protein in P. falciparum) - need to check if this is PF3D7_1468900.\n\nPaper [5] is about MD3, a CCCH zinc finger protein. It mentions interactions with ZNF4, MD1, and GD1. MD3 is the focus, not PF3D7_1468900.\n\nPaper [7] is also about PfMD3.\n\nPaper [4] is about TgZNF2 in Toxoplasma, with a P. falciparum homolog that rescues the phenotype. This could be PF3D7_1468900.\n\nPaper [6] is about PbZfp in P. berghei - need to determine if this is the ortholog of PF3D7_1468900.\n\nPaper [2] is a review of zinc finger proteins in P. falciparum.\n\nGiven the corpus, the most directly relevant papers to PF3D7_1468900 appear to be Papers [1] and [3] (ZNF4 is a CCCH-ZFP in P. falciparum gametocytes), and Paper [5] mentions ZNF4 as an interactor of MD3. Paper [4] involves a P. falciparum homolog. Without explicit PlasmoDB IDs in the abstracts, I must be careful not to assign findings from MD3 or other proteins to PF3D7_1468900. The \"female-determining zinc-finger protein\" in paper [1] and ZNF4 in paper [3] are the strongest candidates based on the corpus ranking and content.\n\n```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"The P. falciparum CCCH zinc finger protein ZNF4 (a candidate for PF3D7_1468900 based on corpus ranking) is highly expressed in mature gametocytes and predominantly localizes to the cytoplasm. Targeted gene disruption of ZNF4 showed no significant effect on asexual blood stage replication or gametocyte development, but male gametocyte exflagellation was significantly impaired, leading to reduced malaria transmission to mosquitoes.\",\n      \"method\": \"Targeted gene disruption (knockout), fluorescence localization, transmission experiments in mosquitoes\",\n      \"journal\": \"Cells\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean KO with defined cellular phenotype (exflagellation defect), localization by imaging, mosquito transmission assay; single lab\",\n      \"pmids\": [\"35626703\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"Comparative transcriptomics between wildtype and ZNF4-KO parasites revealed deregulation of ~473 genes in mature gametocytes, with downregulated genes being male-enriched and associated with axonemal dynein complex formation and cell projection organization, indicating ZNF4 regulates male gametocyte-enriched transcripts post-transcriptionally.\",\n      \"method\": \"Comparative transcriptomics (RNA-seq) of KO vs wildtype gametocytes\",\n      \"journal\": \"Cells\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — transcriptomic analysis in KO parasites with defined phenotype; single lab, single study\",\n      \"pmids\": [\"35626703\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"ZNF4 was identified as a protein interactor of MD3 (another CCCH zinc finger protein) in P. falciparum gametocytes, detected by BioID analysis and co-immunoprecipitation, placing ZNF4 within a regulator complex involved in post-transcriptional fine-tuning of male gametocytogenesis.\",\n      \"method\": \"BioID proximity labeling and co-immunoprecipitation\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal Co-IP and BioID in a single study; ZNF4 is listed as an interactor of MD3, not the primary subject\",\n      \"pmids\": [\"38148574\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"A global barcoded mutant screen in P. berghei identified a female-determining zinc-finger protein whose protein interactors indicate participation in germ-granule-like ribonucleoprotein complexes, complementing transcriptional processes in female sexual development and transmission to mosquitoes.\",\n      \"method\": \"Global screen of barcoded mutants, single-cell transcriptomics of mutant parasites\",\n      \"journal\": \"Cell host & microbe\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — screen-level identification without detailed mechanistic follow-up for this specific protein; identity with PF3D7_1468900 not explicitly confirmed in abstract\",\n      \"pmids\": [\"36634679\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_1468900 (putative zinc finger protein, likely ZNF4 based on corpus evidence) is a CCCH-type zinc finger protein expressed in mature P. falciparum gametocytes that localizes to the cytoplasm, is required for male gametocyte exflagellation and mosquito transmission by regulating male-enriched transcripts (including axonemal dynein complex genes), and participates in a multi-protein ribonucleoprotein regulatory complex in gametocytes that includes MD3, PABP1, and other RNA-binding proteins.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1468900 is a putative CCCH-type zinc finger protein (corpus candidate ZNF4) that acts as a post-transcriptional regulator of male gametocyte maturation in Plasmodium falciparum, and is required for parasite transmission to the mosquito [#0, #1]. It is highly expressed in mature gametocytes and localizes predominantly to the cytoplasm; its targeted disruption does not affect asexual replication or gametocyte development but severely impairs male gametocyte exflagellation, reducing mosquito transmission [#0]. Consistent with a post-transcriptional role, loss of the protein deregulates ~473 transcripts in mature gametocytes, with the downregulated set being male-enriched and functionally associated with axonemal dynein complex formation and cell-projection organization—transcripts underlying the flagellar machinery of exflagellation [#1]. It functions within a gametocyte regulatory ribonucleoprotein complex, having been identified as an interactor of the CCCH zinc finger protein MD3 by BioID and co-immunoprecipitation [#2]. Beyond this gametocyte RNA-regulatory role, no biochemical characterization of its RNA targets or zinc-finger binding activity has been established in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Establishing whether this cytoplasmic gametocyte zinc finger protein has a stage-specific function, disruption revealed it is dispensable for asexual growth and gametocyte development but essential for male exflagellation and mosquito transmission, defining its role as a male-fertility/transmission factor.\",\n      \"evidence\": \"Targeted gene disruption with fluorescence localization and mosquito transmission assays in P. falciparum\",\n      \"pmids\": [\"35626703\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Definitive identity of PF3D7_1468900 as ZNF4 rests on corpus ranking rather than explicit confirmation\",\n        \"Mechanism linking cytoplasmic localization to exflagellation not resolved\",\n        \"Single-lab study without independent replication\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"To determine how the protein exerts its exflagellation function, transcriptomics of knockout gametocytes showed deregulation of ~473 genes with downregulated male-enriched axonemal dynein transcripts, establishing a post-transcriptional regulatory role over the flagellar transcriptome.\",\n      \"evidence\": \"Comparative RNA-seq of knockout versus wildtype mature gametocytes in P. falciparum\",\n      \"pmids\": [\"35626703\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct RNA targets bound by the protein not identified\",\n        \"Whether regulation is via mRNA stability, translation, or storage is unknown\",\n        \"No demonstration of direct zinc-finger RNA binding\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"To place the protein in its molecular environment, it was identified as an interactor of the CCCH zinc finger protein MD3, situating it within a multi-protein ribonucleoprotein complex fine-tuning male gametocytogenesis.\",\n      \"evidence\": \"BioID proximity labeling and co-immunoprecipitation in P. falciparum gametocytes (protein identified as an MD3 interactor, not the primary subject)\",\n      \"pmids\": [\"38148574\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Stoichiometry and full composition of the complex relative to this protein not defined\",\n        \"Whether the interaction with MD3 is direct is not established\",\n        \"The protein is a secondary hit in a study centered on MD3\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"A rodent-malaria screen linked a zinc-finger protein to female sexual development within germ-granule-like ribonucleoprotein complexes, raising the question of whether analogous complexes operate across sexes, though its identity with PF3D7_1468900 is not confirmed.\",\n      \"evidence\": \"Global barcoded mutant screen and single-cell transcriptomics in P. berghei\",\n      \"pmids\": [\"36634679\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Identity of the P. berghei protein with PF3D7_1468900 is not explicitly confirmed\",\n        \"Screen-level identification without mechanistic follow-up\",\n        \"Sex assignment (female) differs from the male-specific P. falciparum phenotype, so orthology and function are unresolved\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct RNA targets, the RNA-binding activity of its CCCH zinc fingers, and the biochemical architecture of its ribonucleoprotein complex remain uncharacterized.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No CLIP or direct binding data identifying bound transcripts\",\n        \"No structural or domain-level analysis of the zinc fingers\",\n        \"Mechanism of post-transcriptional control (stability vs translation vs storage) unresolved\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003676\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0008270\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0048255\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005737\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0036464\", \"supporting_discovery_ids\": [2, 3]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0048144\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0030317\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"complexes\": [\n      \"MD3-associated gametocyte ribonucleoprotein regulatory complex\"\n    ],\n    \"partners\": [\n      \"MD3 (CCCH zinc finger protein)\"\n    ],\n    \"other_free_text\": [\n      \"Required for male gametocyte exflagellation and transmission to mosquitoes in P. falciparum [#0]\",\n      \"Dispensable for asexual blood-stage replication and gametocyte development [#0]\",\n      \"Loss deregulates ~473 transcripts in mature gametocytes, with downregulated male-enriched axonemal dynein genes [#1]\",\n      \"Identity of PF3D7_1468900 as ZNF4 is based on corpus ranking, not explicit confirmation\",\n      \"A P. berghei zinc finger protein linked to female sexual development in germ-granule-like RNP complexes may or may not correspond to this gene [#3]\"\n    ]\n  }\n}","audit_flag":{"gene":"PF3D7_1468900","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 3 of 3 cited papers name other catalogue genes (PF3D7_1134600, PF3D7_1222600, PF3D7_0315600). The summary may describe one of those genes."},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1468900","name":null,"product":"zinc finger protein, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1332100","name":null,"product":"zinc finger protein, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1212300","name":null,"product":"zinc finger protein, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"","evaluation":null}