{"gene":"PF3D7_1460700","run_date":"2026-09-08T14:19:07","timeline":{"discoveries":[{"year":2014,"finding":"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution resolved the large (60S) subunit, within which L27 (PF3D7_1460700) is a constituent ribosomal protein, establishing its structural context within the native parasite ribosome complex.","method":"Cryo-electron microscopy at 3.2 Å resolution of the P. falciparum 80S ribosome","journal":"eLife","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — high-resolution cryo-EM structure of the intact ribosome, but no finding specific to L27 beyond its membership in the 60S subunit of the complex; single study","pmids":["24913268"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"finding":"In situ cryo-electron tomography of P. falciparum-infected erythrocytes resolved eight high-resolution structures of the native malarial ribosome in actively translating states, placing L27 (PF3D7_1460700) within the 60S subunit in its physiological, cellular context during translation elongation.","method":"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes","journal":"Nature structural & molecular biology","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — state-of-the-art in-cell structural biology, but no finding specific to L27 beyond its presence in the 60S subunit of the ribosome complex; single study","pmids":["40825879"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_1460700 (L27) is established as a constituent protein of the 60S large subunit of the P. falciparum 80S cytoplasmic ribosome, as visualized by cryo-EM and in situ cryo-electron tomography; no findings specific to its individual function, interactions, or mechanism beyond ribosome subunit membership are available in the current literature."},"narrative":{"mechanistic_narrative":"PF3D7_1460700 (L27) is a constituent ribosomal protein of the 60S large subunit of the Plasmodium falciparum 80S cytoplasmic ribosome, resolved as part of the native parasite ribosome complex by cryo-EM at 3.2 Å resolution [PMID:24913268] and localized within the 60S subunit of actively translating ribosomes by in situ cryo-electron tomography inside infected erythrocytes [PMID:40825879]. Beyond its membership in the 60S subunit of the ribosome, no function, interaction partner, or mechanism specific to L27 itself has been characterized in the available corpus.","teleology":[{"year":2014,"claim":"Whether L27 is a bona fide component of the parasite ribosome and where it sits structurally was addressed by resolving the intact P. falciparum 80S ribosome, establishing L27 as a 60S subunit constituent.","evidence":"Cryo-electron microscopy of the P. falciparum 80S ribosome at 3.2 Å resolution","pmids":["24913268"],"confidence":"Medium","gaps":["No function or activity specific to L27 distinguished from bulk ribosome structure","No interaction partners or regulatory role for L27 individually established","Structure of purified complex, not in a cellular translation context"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"claim":"Whether L27's structural placement holds in the physiological, translating ribosome was addressed by in-cell structural biology, confirming L27 within the 60S subunit of natively translating malarial ribosomes.","evidence":"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes, yielding eight structures of the elongating ribosome","pmids":["40825879"],"confidence":"Medium","gaps":["No L27-specific functional contribution to translation elongation defined","No evidence for any role of L27 outside the ribosome complex","Single study; no perturbation of L27 to test its requirement"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"It remains unknown whether L27 has any function, interaction, or phenotype distinct from its structural membership in the 60S subunit.","evidence":"No experiment in the corpus addresses L27 individually","pmids":[],"confidence":"Medium","gaps":["No genetic knockdown/knockout of L27 to assess essentiality","No biochemical or interaction data specific to L27","No evidence of extraribosomal roles"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["60S large ribosomal subunit of the P. falciparum 80S cytoplasmic ribosome"],"partners":[],"other_free_text":["The corpus establishes only membership of PF3D7_1460700 (L27) in the 60S subunit of the ribosome; no function, interaction, or mechanism specific to this gene beyond structural constituency has been characterized."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"24913268","id":"PMC_24913268","title":"Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.","date":"2014","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/24913268","citation_count":272,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"28288098","id":"PMC_28288098","title":"Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis.","date":"2017","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/28288098","citation_count":136,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"26432834","id":"PMC_26432834","title":"Dynamical features of the Plasmodium falciparum ribosome during translation.","date":"2015","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/26432834","citation_count":45,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40825879","id":"PMC_40825879","title":"Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug.","date":"2025","source":"Nature structural & molecular biology","url":"https://pubmed.ncbi.nlm.nih.gov/40825879","citation_count":7,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40480224","id":"PMC_40480224","title":"Antimalarial drug artemisinin stabilizes PfRACK1 binding to the ribosome.","date":"2025","source":"Structure (London, England : 1993)","url":"https://pubmed.ncbi.nlm.nih.gov/40480224","citation_count":3,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":9674,"output_tokens":1130,"usd":0.034983,"retried_sync":true},"stage2":{"model":"claude-opus-4-8","input_tokens":5202,"output_tokens":1374,"usd":0.03018,"stage2_stop_reason":"end_turn"},"total_usd":0.065163,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2014,\n      \"finding\": \"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution resolved the large (60S) subunit, within which L27 (PF3D7_1460700) is a constituent ribosomal protein, establishing its structural context within the native parasite ribosome complex.\",\n      \"method\": \"Cryo-electron microscopy at 3.2 Å resolution of the P. falciparum 80S ribosome\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — high-resolution cryo-EM structure of the intact ribosome, but no finding specific to L27 beyond its membership in the 60S subunit of the complex; single study\",\n      \"pmids\": [\"24913268\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"In situ cryo-electron tomography of P. falciparum-infected erythrocytes resolved eight high-resolution structures of the native malarial ribosome in actively translating states, placing L27 (PF3D7_1460700) within the 60S subunit in its physiological, cellular context during translation elongation.\",\n      \"method\": \"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes\",\n      \"journal\": \"Nature structural & molecular biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — state-of-the-art in-cell structural biology, but no finding specific to L27 beyond its presence in the 60S subunit of the ribosome complex; single study\",\n      \"pmids\": [\"40825879\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_1460700 (L27) is established as a constituent protein of the 60S large subunit of the P. falciparum 80S cytoplasmic ribosome, as visualized by cryo-EM and in situ cryo-electron tomography; no findings specific to its individual function, interactions, or mechanism beyond ribosome subunit membership are available in the current literature.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1460700 (L27) is a constituent ribosomal protein of the 60S large subunit of the Plasmodium falciparum 80S cytoplasmic ribosome, resolved as part of the native parasite ribosome complex by cryo-EM at 3.2 Å resolution [#0] and localized within the 60S subunit of actively translating ribosomes by in situ cryo-electron tomography inside infected erythrocytes [#1]. Beyond its membership in the 60S subunit of the ribosome, no function, interaction partner, or mechanism specific to L27 itself has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2014,\n      \"claim\": \"Whether L27 is a bona fide component of the parasite ribosome and where it sits structurally was addressed by resolving the intact P. falciparum 80S ribosome, establishing L27 as a 60S subunit constituent.\",\n      \"evidence\": \"Cryo-electron microscopy of the P. falciparum 80S ribosome at 3.2 Å resolution\",\n      \"pmids\": [\"24913268\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No function or activity specific to L27 distinguished from bulk ribosome structure\",\n        \"No interaction partners or regulatory role for L27 individually established\",\n        \"Structure of purified complex, not in a cellular translation context\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Whether L27's structural placement holds in the physiological, translating ribosome was addressed by in-cell structural biology, confirming L27 within the 60S subunit of natively translating malarial ribosomes.\",\n      \"evidence\": \"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes, yielding eight structures of the elongating ribosome\",\n      \"pmids\": [\"40825879\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No L27-specific functional contribution to translation elongation defined\",\n        \"No evidence for any role of L27 outside the ribosome complex\",\n        \"Single study; no perturbation of L27 to test its requirement\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown whether L27 has any function, interaction, or phenotype distinct from its structural membership in the 60S subunit.\",\n      \"evidence\": \"No experiment in the corpus addresses L27 individually\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No genetic knockdown/knockout of L27 to assess essentiality\",\n        \"No biochemical or interaction data specific to L27\",\n        \"No evidence of extraribosomal roles\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003735\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0022625\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0005737\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006414\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"complexes\": [\n      \"60S large ribosomal subunit of the P. falciparum 80S cytoplasmic ribosome\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"The corpus establishes only membership of PF3D7_1460700 (L27) in the 60S subunit of the ribosome; no function, interaction, or mechanism specific to this gene beyond structural constituency has been characterized.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1460700","name":null,"product":"60S ribosomal protein L27","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1324400","name":null,"product":"60S ribosomal protein L27, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1221000","name":null,"product":"60S ribosomal protein L27, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"","evaluation":null}