{"gene":"PF3D7_1449500","run_date":"2026-09-08T14:19:07","timeline":{"discoveries":[],"current_model":"No mechanistic findings about PF3D7_1449500 (AP2-O5) are present in the available literature; the corpus is dominated by studies of other ApiAP2 family members (AP2-G, AP2-O, AP2-G2, AP2-I, AP2-SP, AP2-G5, etc.) and does not contain any direct experimental characterization of PF3D7_1449500 itself."},"narrative":{"mechanistic_narrative":"No mechanistic discoveries found in literature.","teleology":[],"mechanism_profile":null},"prefetch_data":{},"corpus_meta":[{"pmid":"24572369","id":"PMC_24572369","title":"A transcriptional switch underlies commitment to sexual development in malaria 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evolution","url":"https://pubmed.ncbi.nlm.nih.gov/29255304","citation_count":59,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36634679","id":"PMC_36634679","title":"Regulators of male and female sexual development are critical for the transmission of a malaria parasite.","date":"2023","source":"Cell host & microbe","url":"https://pubmed.ncbi.nlm.nih.gov/36634679","citation_count":58,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"19913037","id":"PMC_19913037","title":"Structural determinants of DNA binding by a P. falciparum ApiAP2 transcriptional regulator.","date":"2009","source":"Journal of molecular biology","url":"https://pubmed.ncbi.nlm.nih.gov/19913037","citation_count":57,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34365503","id":"PMC_34365503","title":"A cascade of transcriptional repression determines sexual commitment and development in Plasmodium falciparum.","date":"2021","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/34365503","citation_count":49,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfAP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"20657661","id":"PMC_20657661","title":"The construction and use of log-odds substitution scores for multiple sequence alignment.","date":"2010","source":"PLoS computational 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microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/34400833","citation_count":48,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfAP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"33425787","id":"PMC_33425787","title":"From Genes to Transcripts, a Tightly Regulated Journey in Plasmodium.","date":"2020","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/33425787","citation_count":46,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34403450","id":"PMC_34403450","title":"A unique GCN5 histone acetyltransferase complex controls erythrocyte invasion and virulence in the malaria parasite Plasmodium falciparum.","date":"2021","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/34403450","citation_count":44,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2","PfAP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37026483","id":"PMC_37026483","title":"Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation.","date":"2023","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/37026483","citation_count":25,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35947628","id":"PMC_35947628","title":"Identification of a novel AP2 transcription factor in zygotes with an essential role in Plasmodium ookinete development.","date":"2022","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/35947628","citation_count":22,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42120403","id":"PMC_42120403","title":"A divergent Plasmodium NEK4 acts as a key regulator driving the early events of meiosis.","date":"2026","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/42120403","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41332573","id":"PMC_41332573","title":"A divergent Plasmodium NEK4 acts as a key regulator driving the early events of meiosis.","date":"2025","source":"bioRxiv : the preprint server for biology","url":"https://pubmed.ncbi.nlm.nih.gov/41332573","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41510253","id":"PMC_41510253","title":"A divergent Plasmodium NEK4 acts as a key regulator driving the early events of meiosis.","date":"2025","source":"Research square","url":"https://pubmed.ncbi.nlm.nih.gov/41510253","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":22574,"output_tokens":594,"usd":0.038316,"stage1_stop_reason":"end_turn"},"stage2":{"model":"","input_tokens":0,"output_tokens":0,"usd":0.0},"total_usd":0.038316,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"Looking through the abstracts, I need to identify papers that specifically discuss PF3D7_1449500 (AP2-O5, gene symbol O5).\n\nScanning the corpus:\n- Most papers discuss AP2-G, AP2-G2, AP2-O, AP2-I, AP2-SP, AP2-L, AP2-HS, AP2-Z, SIP2, PfAP2-G5 (PF3D7_1139300), and other ApiAP2 family members — these are sibling family members, NOT PF3D7_1449500.\n- Papers [42], [43], [44] mention \"AP2-O\" being affected downstream of NEK4, but this refers to AP2-O (the ookinete transcription factor), not AP2-O5 (PF3D7_1449500).\n- Paper [10] (Modrzynska et al., 2017) mentions a systematic knockout screen and mentions \"AP2-O\" and \"AP2-SP\" having non-essential functions in blood stages — but this refers to AP2-O, not AP2-O5.\n- Paper [34] mentions PfAP2-G5 (PF3D7_1139300) explicitly — this is a different gene from PF3D7_1449500.\n- Paper [8] (Poran et al., 2017) mentions \"other AP2 transcription factors\" being upregulated in sexually committed schizonts but doesn't specifically identify PF3D7_1449500.\n- Paper [25] (Zhang et al., 2017) studies P. yoelii ApiAP2 genes systematically but doesn't specifically identify the O5 ortholog by name in the abstract.\n\nNone of the abstracts in the corpus explicitly name, cite the locus ID, or describe direct experiments on PF3D7_1449500 (AP2-O5). The corpus is dominated by other ApiAP2 family members.\n\n```json\n{\n  \"discoveries\": [],\n  \"current_model\": \"No mechanistic findings about PF3D7_1449500 (AP2-O5) are present in the available literature; the corpus is dominated by studies of other ApiAP2 family members (AP2-G, AP2-O, AP2-G2, AP2-I, AP2-SP, AP2-G5, etc.) and does not contain any direct experimental characterization of PF3D7_1449500 itself.\"\n}\n```","stage2_raw":"","audit_flag":{"gene":"PF3D7_1449500","tier":"COVERAGE","verdict":"Coverage note","subtype":"corpus_unverifiable","uniprot_band":"empty","rules_fired":"R1","issue":"R1: record has no curated symbol, so its corpus cannot name it (named in 0/100); grounding untestable — coverage note, not a collision"},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1449500","name":null,"product":"AP2 domain transcription factor AP2-O5, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1313200","name":null,"product":"AP2 domain transcription factor AP2-O5, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1232600","name":null,"product":"AP2 domain transcription factor AP2-O5, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AP2-O5","evaluation":null}