{"gene":"PF3D7_1444500","run_date":"2026-09-08T14:19:07","timeline":{"discoveries":[{"year":2009,"finding":"eIK1 (PfeIK1) phosphorylates recombinant P. falciparum eIF2alpha in vitro, demonstrating it is a bona fide eIF2alpha kinase; genetic disruption of pfeik1 abolishes eIF2alpha phosphorylation in response to amino-acid starvation in asexual parasites, placing eIK1 as the primary kinase mediating starvation-induced eIF2alpha phosphorylation.","method":"In vitro kinase assay with recombinant protein; pfeik1 gene disruption (reverse genetics) with immunological readout of eIF2alpha phosphorylation state under starvation","journal":"Malaria journal","confidence":"High","confidence_rationale":"Tier 1-2 / Moderate — in vitro kinase assay with recombinant substrate plus clean genetic knockout with specific phosphorylation phenotype, single lab but two orthogonal methods","pmids":["19435497"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2009,"finding":"eIK1 (PfeIK1) is not required for asexual or sexual development of P. falciparum; pfeik1-knockout parasites develop normally through asexual stages and into sporozoites.","method":"Reverse genetics (pfeik1 gene disruption); parasite development assay through sexual stages to sporozoites","journal":"Malaria journal","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean knockout with developmental stage readout, single lab, two orthogonal stage assessments","pmids":["19435497"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2020,"finding":"eIK1 (PfeIK1) is required for melatonin-induced increases in parasitemia; pfeik1-knockout P. falciparum parasites fail to show the melatonin-dependent increase in parasitemia seen in wild-type parasites, placing eIK1 as a central component of melatonin downstream signaling in parasite cell cycle progression.","method":"pfeik1 knockout parasites; flow cytometry with MitoTracker Deep Red and SYBR Green labeling; comparative analysis of WT vs. knockout response to melatonin","journal":"Journal of pineal research","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — clean knockout with specific melatonin phenotype, single lab, single primary method (flow cytometry)","pmids":["32702775"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":"3D7"}]},{"year":2023,"finding":"eIK1 is identified as critical for Plasmodium sensing and responding to specific amino-acid-limiting conditions; phenotypic screening of kinase-knockout mutants demonstrated that loss of eIK1 impairs the parasite's ability to sense and respond to distinct AA-limiting conditions, and this sensing pathway is temporally regulated across life cycle stages.","method":"Phenotypic screen of kinase knockout mutant parasites under amino-acid limiting conditions; comparative growth/survival assays across life cycle stages","journal":"Communications biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — genetic knockout with defined phenotypic readout across multiple conditions, single study, single primary experimental approach","pmids":["36810637"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2019,"finding":"eIK1 (PfeIK1) and eIF2alpha signaling are NOT required for the adaptive proline response (APR) or halofuginone (HFG) activity in P. falciparum; comparative studies with pfeik1-knockout parasites showed that HFG activity and APR are independent of PfeIK1.","method":"Comparative assays using pfeik1-knockout parasite line vs. wild-type under halofuginone treatment; measurement of intracellular proline and eIF2alpha phosphorylation","journal":"ACS infectious diseases","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — negative finding established with clean knockout and multiple biochemical readouts, single lab","pmids":["30773881"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"eIK1 (PF3D7_1444500) is a P. falciparum eIF2alpha kinase that directly phosphorylates eIF2alpha in vitro and in vivo, mediates eIF2alpha phosphorylation specifically in response to amino-acid starvation and functions in a broader AA-sensing pathway critical for parasite adaptation to nutrient stress; it is also required for melatonin-dependent cell cycle synchronization signaling, but is dispensable for asexual and sexual development under normal conditions and is not involved in the adaptive proline response."},"narrative":{"mechanistic_narrative":"eIK1 (PF3D7_1444500) is a P. falciparum eIF2alpha kinase that couples nutrient sensing to translational control during the parasite's intraerythrocytic life cycle [PMID:19435497, PMID:36810637]. It directly phosphorylates recombinant P. falciparum eIF2alpha in vitro, and its genetic disruption abolishes eIF2alpha phosphorylation triggered by amino-acid starvation, establishing it as the primary kinase mediating starvation-induced eIF2alpha phosphorylation [PMID:19435497]. Consistent with this role in a broader amino-acid-sensing pathway, loss of eIK1 impairs the parasite's ability to sense and respond to distinct amino-acid-limiting conditions, and this sensing is temporally regulated across life cycle stages [PMID:36810637]. eIK1 is also a central component of melatonin-dependent signaling controlling cell cycle progression, as pfeik1-knockout parasites fail to mount the melatonin-induced increase in parasitemia seen in wild-type [PMID:32702775]. eIK1 is dispensable for asexual and sexual development under normal conditions [PMID:19435497] and is not involved in the adaptive proline response or halofuginone activity, which proceed independently of eIK1 and eIF2alpha signaling [PMID:30773881]. Beyond these functional and phenotypic characterizations, no structural model, interaction partners, or upstream activating mechanism for eIK1 have been characterized in the available corpus.","teleology":[{"year":2009,"claim":"Whether eIK1 is a genuine eIF2alpha kinase and whether it mediates a specific stress response was unknown; establishing this defined its molecular activity and physiological trigger.","evidence":"In vitro kinase assay with recombinant eIF2alpha plus pfeik1 gene disruption with phosphorylation readout under amino-acid starvation in P. falciparum","pmids":["19435497"],"confidence":"High","gaps":["Upstream activator sensing amino-acid depletion not identified","No structural or domain-level basis for substrate recognition","Whether other kinases redundantly phosphorylate eIF2alpha under other stresses not resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2009,"claim":"It was unclear whether eIK1 is essential for parasite development; testing knockout progression established that its function is conditional rather than constitutive.","evidence":"Reverse genetics (pfeik1 disruption) with developmental staging from asexual stages through sporozoites in P. falciparum","pmids":["19435497"],"confidence":"Medium","gaps":["Dispensability tested only under normal (non-stress) conditions","No quantitative comparison of fitness under nutrient limitation in this assay"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2019,"claim":"Whether eIK1/eIF2alpha signaling underlies the adaptive proline response and halofuginone action was open; testing showed this drug-relevant pathway is eIK1-independent, narrowing eIK1's functional scope.","evidence":"Comparative halofuginone treatment and proline/eIF2alpha phosphorylation measurements in pfeik1-knockout vs. wild-type P. falciparum","pmids":["30773881"],"confidence":"Medium","gaps":["Does not identify which kinase mediates the adaptive proline response","Negative result confined to proline/halofuginone context"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2020,"claim":"The downstream effectors of melatonin-driven cell cycle synchronization were poorly defined; showing eIK1 is required for the melatonin parasitemia response placed it in this signaling axis.","evidence":"Flow cytometry (MitoTracker Deep Red/SYBR Green) comparing melatonin response of WT vs. pfeik1-knockout P. falciparum 3D7","pmids":["32702775"],"confidence":"Medium","gaps":["Single primary method (flow cytometry)","Mechanistic link between melatonin sensing and eIK1 activation unknown","Whether eIF2alpha phosphorylation mediates the melatonin phenotype not shown"],"attribution":[{"species":"Plasmodium falciparum","strain":"3D7"}]},{"year":2023,"claim":"Whether eIK1 discriminates among distinct nutrient-limiting conditions was unresolved; a kinase-knockout phenotypic screen showed eIK1 is required for sensing specific amino-acid limitations in a stage-dependent manner.","evidence":"Phenotypic screen of kinase-knockout mutants under amino-acid-limiting conditions with growth/survival assays across life cycle stages in P. falciparum","pmids":["36810637"],"confidence":"Medium","gaps":["Which specific amino acids are sensed via eIK1 not fully delineated","Molecular sensor mechanism upstream of eIK1 unknown","Single study, single primary approach"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"How eIK1 is activated by nutrient and melatonin signals at the molecular level, and how these two contexts converge on eIF2alpha, remains unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No upstream sensor or activating ligand/domain identified","No structural model of eIK1","No interaction partners defined in the corpus"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["P. FALCIPARUM EIF2ALPHA (SUBSTRATE)"],"other_free_text":["Primary kinase mediating amino-acid-starvation-induced eIF2alpha phosphorylation in asexual P. falciparum [#0]","Dispensable for asexual and sexual development under normal conditions [#1]","Required for melatonin-induced increase in parasitemia / cell cycle signaling [#2]","Required for sensing specific amino-acid-limiting conditions in a stage-dependent manner [#3]","Not required for the adaptive proline response or halofuginone activity [#4]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"15479470","id":"PMC_15479470","title":"Protein kinases of the human malaria parasite Plasmodium falciparum: the kinome of a divergent eukaryote.","date":"2004","source":"BMC genomics","url":"https://pubmed.ncbi.nlm.nih.gov/15479470","citation_count":421,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"19435497","id":"PMC_19435497","title":"PfeIK1, a eukaryotic initiation factor 2alpha kinase of the human malaria parasite Plasmodium falciparum, regulates stress-response to amino-acid starvation.","date":"2009","source":"Malaria journal","url":"https://pubmed.ncbi.nlm.nih.gov/19435497","citation_count":77,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfeIK1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"32702775","id":"PMC_32702775","title":"The Plasmodium falciparum eIK1 kinase (PfeIK1) is central for melatonin synchronization in the human malaria parasite. Melatotosil blocks melatonin action on parasite cell cycle.","date":"2020","source":"Journal of pineal research","url":"https://pubmed.ncbi.nlm.nih.gov/32702775","citation_count":15,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["eIK1","PfeIK1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36810637","id":"PMC_36810637","title":"A non-canonical sensing pathway mediates Plasmodium adaptation to amino acid deficiency.","date":"2023","source":"Communications biology","url":"https://pubmed.ncbi.nlm.nih.gov/36810637","citation_count":13,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["eIK1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"30773881","id":"PMC_30773881","title":"The Adaptive Proline Response in P. falciparum Is Independent of PfeIK1 and eIF2α Signaling.","date":"2019","source":"ACS infectious diseases","url":"https://pubmed.ncbi.nlm.nih.gov/30773881","citation_count":12,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfeIK1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34428626","id":"PMC_34428626","title":"Molecular basis of synchronous replication of malaria parasites in the blood stage.","date":"2021","source":"Current opinion in microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/34428626","citation_count":4,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfeIK1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":5735,"output_tokens":1447,"usd":0.019455,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6419,"output_tokens":2552,"usd":0.047947,"stage2_stop_reason":"end_turn"},"total_usd":0.067402,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2009,\n      \"finding\": \"eIK1 (PfeIK1) phosphorylates recombinant P. falciparum eIF2alpha in vitro, demonstrating it is a bona fide eIF2alpha kinase; genetic disruption of pfeik1 abolishes eIF2alpha phosphorylation in response to amino-acid starvation in asexual parasites, placing eIK1 as the primary kinase mediating starvation-induced eIF2alpha phosphorylation.\",\n      \"method\": \"In vitro kinase assay with recombinant protein; pfeik1 gene disruption (reverse genetics) with immunological readout of eIF2alpha phosphorylation state under starvation\",\n      \"journal\": \"Malaria journal\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1-2 / Moderate — in vitro kinase assay with recombinant substrate plus clean genetic knockout with specific phosphorylation phenotype, single lab but two orthogonal methods\",\n      \"pmids\": [\"19435497\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2009,\n      \"finding\": \"eIK1 (PfeIK1) is not required for asexual or sexual development of P. falciparum; pfeik1-knockout parasites develop normally through asexual stages and into sporozoites.\",\n      \"method\": \"Reverse genetics (pfeik1 gene disruption); parasite development assay through sexual stages to sporozoites\",\n      \"journal\": \"Malaria journal\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean knockout with developmental stage readout, single lab, two orthogonal stage assessments\",\n      \"pmids\": [\"19435497\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"eIK1 (PfeIK1) is required for melatonin-induced increases in parasitemia; pfeik1-knockout P. falciparum parasites fail to show the melatonin-dependent increase in parasitemia seen in wild-type parasites, placing eIK1 as a central component of melatonin downstream signaling in parasite cell cycle progression.\",\n      \"method\": \"pfeik1 knockout parasites; flow cytometry with MitoTracker Deep Red and SYBR Green labeling; comparative analysis of WT vs. knockout response to melatonin\",\n      \"journal\": \"Journal of pineal research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — clean knockout with specific melatonin phenotype, single lab, single primary method (flow cytometry)\",\n      \"pmids\": [\"32702775\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"3D7\"}\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"eIK1 is identified as critical for Plasmodium sensing and responding to specific amino-acid-limiting conditions; phenotypic screening of kinase-knockout mutants demonstrated that loss of eIK1 impairs the parasite's ability to sense and respond to distinct AA-limiting conditions, and this sensing pathway is temporally regulated across life cycle stages.\",\n      \"method\": \"Phenotypic screen of kinase knockout mutant parasites under amino-acid limiting conditions; comparative growth/survival assays across life cycle stages\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — genetic knockout with defined phenotypic readout across multiple conditions, single study, single primary experimental approach\",\n      \"pmids\": [\"36810637\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"eIK1 (PfeIK1) and eIF2alpha signaling are NOT required for the adaptive proline response (APR) or halofuginone (HFG) activity in P. falciparum; comparative studies with pfeik1-knockout parasites showed that HFG activity and APR are independent of PfeIK1.\",\n      \"method\": \"Comparative assays using pfeik1-knockout parasite line vs. wild-type under halofuginone treatment; measurement of intracellular proline and eIF2alpha phosphorylation\",\n      \"journal\": \"ACS infectious diseases\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — negative finding established with clean knockout and multiple biochemical readouts, single lab\",\n      \"pmids\": [\"30773881\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"eIK1 (PF3D7_1444500) is a P. falciparum eIF2alpha kinase that directly phosphorylates eIF2alpha in vitro and in vivo, mediates eIF2alpha phosphorylation specifically in response to amino-acid starvation and functions in a broader AA-sensing pathway critical for parasite adaptation to nutrient stress; it is also required for melatonin-dependent cell cycle synchronization signaling, but is dispensable for asexual and sexual development under normal conditions and is not involved in the adaptive proline response.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"eIK1 (PF3D7_1444500) is a P. falciparum eIF2alpha kinase that couples nutrient sensing to translational control during the parasite's intraerythrocytic life cycle [#0, #3]. It directly phosphorylates recombinant P. falciparum eIF2alpha in vitro, and its genetic disruption abolishes eIF2alpha phosphorylation triggered by amino-acid starvation, establishing it as the primary kinase mediating starvation-induced eIF2alpha phosphorylation [#0]. Consistent with this role in a broader amino-acid-sensing pathway, loss of eIK1 impairs the parasite's ability to sense and respond to distinct amino-acid-limiting conditions, and this sensing is temporally regulated across life cycle stages [#3]. eIK1 is also a central component of melatonin-dependent signaling controlling cell cycle progression, as pfeik1-knockout parasites fail to mount the melatonin-induced increase in parasitemia seen in wild-type [#2]. eIK1 is dispensable for asexual and sexual development under normal conditions [#1] and is not involved in the adaptive proline response or halofuginone activity, which proceed independently of eIK1 and eIF2alpha signaling [#4]. Beyond these functional and phenotypic characterizations, no structural model, interaction partners, or upstream activating mechanism for eIK1 have been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2009,\n      \"claim\": \"Whether eIK1 is a genuine eIF2alpha kinase and whether it mediates a specific stress response was unknown; establishing this defined its molecular activity and physiological trigger.\",\n      \"evidence\": \"In vitro kinase assay with recombinant eIF2alpha plus pfeik1 gene disruption with phosphorylation readout under amino-acid starvation in P. falciparum\",\n      \"pmids\": [\"19435497\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Upstream activator sensing amino-acid depletion not identified\", \"No structural or domain-level basis for substrate recognition\", \"Whether other kinases redundantly phosphorylate eIF2alpha under other stresses not resolved\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2009,\n      \"claim\": \"It was unclear whether eIK1 is essential for parasite development; testing knockout progression established that its function is conditional rather than constitutive.\",\n      \"evidence\": \"Reverse genetics (pfeik1 disruption) with developmental staging from asexual stages through sporozoites in P. falciparum\",\n      \"pmids\": [\"19435497\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Dispensability tested only under normal (non-stress) conditions\", \"No quantitative comparison of fitness under nutrient limitation in this assay\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2019,\n      \"claim\": \"Whether eIK1/eIF2alpha signaling underlies the adaptive proline response and halofuginone action was open; testing showed this drug-relevant pathway is eIK1-independent, narrowing eIK1's functional scope.\",\n      \"evidence\": \"Comparative halofuginone treatment and proline/eIF2alpha phosphorylation measurements in pfeik1-knockout vs. wild-type P. falciparum\",\n      \"pmids\": [\"30773881\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Does not identify which kinase mediates the adaptive proline response\", \"Negative result confined to proline/halofuginone context\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2020,\n      \"claim\": \"The downstream effectors of melatonin-driven cell cycle synchronization were poorly defined; showing eIK1 is required for the melatonin parasitemia response placed it in this signaling axis.\",\n      \"evidence\": \"Flow cytometry (MitoTracker Deep Red/SYBR Green) comparing melatonin response of WT vs. pfeik1-knockout P. falciparum 3D7\",\n      \"pmids\": [\"32702775\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Single primary method (flow cytometry)\", \"Mechanistic link between melatonin sensing and eIK1 activation unknown\", \"Whether eIF2alpha phosphorylation mediates the melatonin phenotype not shown\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"3D7\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Whether eIK1 discriminates among distinct nutrient-limiting conditions was unresolved; a kinase-knockout phenotypic screen showed eIK1 is required for sensing specific amino-acid limitations in a stage-dependent manner.\",\n      \"evidence\": \"Phenotypic screen of kinase-knockout mutants under amino-acid-limiting conditions with growth/survival assays across life cycle stages in P. falciparum\",\n      \"pmids\": [\"36810637\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Which specific amino acids are sensed via eIK1 not fully delineated\", \"Molecular sensor mechanism upstream of eIK1 unknown\", \"Single study, single primary approach\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How eIK1 is activated by nutrient and melatonin signals at the molecular level, and how these two contexts converge on eIF2alpha, remains unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"No upstream sensor or activating ligand/domain identified\", \"No structural model of eIK1\", \"No interaction partners defined in the corpus\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0004694\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0004672\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"localization\": [],\n    \"pathway\": [\n      {\"term_id\": \"GO:0140469\", \"supporting_discovery_ids\": [0, 3]},\n      {\"term_id\": \"GO:0009267\", \"supporting_discovery_ids\": [0, 3]},\n      {\"term_id\": \"GO:0007623\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\"P. falciparum eIF2alpha (substrate)\"],\n    \"other_free_text\": [\n      \"Primary kinase mediating amino-acid-starvation-induced eIF2alpha phosphorylation in asexual P. falciparum [#0]\",\n      \"Dispensable for asexual and sexual development under normal conditions [#1]\",\n      \"Required for melatonin-induced increase in parasitemia / cell cycle signaling [#2]\",\n      \"Required for sensing specific amino-acid-limiting conditions in a stage-dependent manner [#3]\",\n      \"Not required for the adaptive proline response or halofuginone activity [#4]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1444500","name":null,"product":"eukaryotic translation initiation factor 2-alpha kinase 1","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1308400","name":null,"product":"eukaryotic translation initiation factor 2-alpha kinase 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1237800","name":null,"product":"eukaryotic translation initiation factor 2-alpha kinase 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"eIK1","evaluation":null}