{"gene":"PF3D7_1420200","run_date":"2026-09-08T14:19:07","timeline":{"discoveries":[{"year":2017,"finding":"PF3D7_1420200 (UNC), a UCS-family myosin chaperone from Plasmodium spp., was required to express milligram quantities of functional full-length PfMyoA in a baculovirus/Sf9 system, establishing its role as a chaperone necessary for proper folding/production of the class XIV myosin A motor.","method":"Recombinant protein expression in baculovirus/Sf9 system requiring co-expression of UCS chaperone to produce functional PfMyoA","journal":"The Journal of biological chemistry","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — functional reconstitution in a single study, single lab, single method demonstrating chaperone requirement","pmids":["28978649"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_1420200 (UNC) is a UCS-family myosin-specific chaperone in Plasmodium falciparum that is functionally required for the production of folded, active PfMyoA (class XIV myosin A), the core motor of the glideosome."},"narrative":{"mechanistic_narrative":"PF3D7_1420200 (UNC) is a UCS-family myosin-specific chaperone in Plasmodium falciparum required for the production of folded, functional PfMyoA (class XIV myosin A) [PMID:28978649]. Co-expression of UNC was necessary to obtain milligram quantities of active full-length PfMyoA in a baculovirus/Sf9 recombinant system, establishing that UNC acts in the folding/production of this myosin motor [PMID:28978649]. Beyond this chaperone requirement demonstrated in a single reconstitution study, no further mechanistic detail — including the structural basis of substrate recognition, cellular localization, or in vivo function of UNC — has been characterized in the available corpus.","teleology":[{"year":2017,"claim":"It was unknown whether PfMyoA required a dedicated chaperone to fold into an active motor; co-expression experiments showed the UCS-family chaperone UNC is necessary to produce functional PfMyoA, establishing UNC as the myosin-specific folding factor for the class XIV motor.","evidence":"Recombinant expression of full-length PfMyoA in a baculovirus/Sf9 system requiring co-expression of the UCS chaperone","pmids":["28978649"],"confidence":"Medium","gaps":["Single study, single lab, single method — chaperone requirement not independently reconstituted","Structural basis of UNC binding to and folding of PfMyoA not defined","In vivo requirement and localization of UNC in the parasite not tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"How UNC recognizes and folds PfMyoA at the molecular level, and whether it is required for glideosome function and parasite motility in vivo, remains unresolved.","evidence":"No further discoveries in the available corpus","pmids":[],"confidence":"Medium","gaps":["No structural model of the UNC–PfMyoA interaction","No genetic loss-of-function or localization data in the parasite","No evidence on whether UNC chaperones other myosins"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["PFMYOA (CLASS XIV MYOSIN A)"],"other_free_text":["UCS-family myosin-specific chaperone required for folding/production of functional PfMyoA [#0]","Demonstrated only via recombinant co-expression in a baculovirus/Sf9 system [#0]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"26769898","id":"PMC_26769898","title":"Structural and functional dissection of Toxoplasma gondii armadillo repeats only protein.","date":"2016","source":"Journal of cell science","url":"https://pubmed.ncbi.nlm.nih.gov/26769898","citation_count":27,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UNC"],"competing_organism_flag":true,"competing_organism_match":"Toxoplasma","competing_organism_only":true},{"pmid":"28978649","id":"PMC_28978649","title":"Reconstitution of the core of the malaria parasite glideosome with recombinant Plasmodium class XIV myosin A and Plasmodium actin.","date":"2017","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/28978649","citation_count":26,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UNC"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3932,"output_tokens":363,"usd":0.00862,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4818,"output_tokens":1052,"usd":0.025195,"stage2_stop_reason":"end_turn"},"total_usd":0.033815,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"PF3D7_1420200 (UNC), a UCS-family myosin chaperone from Plasmodium spp., was required to express milligram quantities of functional full-length PfMyoA in a baculovirus/Sf9 system, establishing its role as a chaperone necessary for proper folding/production of the class XIV myosin A motor.\",\n      \"method\": \"Recombinant protein expression in baculovirus/Sf9 system requiring co-expression of UCS chaperone to produce functional PfMyoA\",\n      \"journal\": \"The Journal of biological chemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — functional reconstitution in a single study, single lab, single method demonstrating chaperone requirement\",\n      \"pmids\": [\"28978649\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_1420200 (UNC) is a UCS-family myosin-specific chaperone in Plasmodium falciparum that is functionally required for the production of folded, active PfMyoA (class XIV myosin A), the core motor of the glideosome.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1420200 (UNC) is a UCS-family myosin-specific chaperone in Plasmodium falciparum required for the production of folded, functional PfMyoA (class XIV myosin A) [#0]. Co-expression of UNC was necessary to obtain milligram quantities of active full-length PfMyoA in a baculovirus/Sf9 recombinant system, establishing that UNC acts in the folding/production of this myosin motor [#0]. Beyond this chaperone requirement demonstrated in a single reconstitution study, no further mechanistic detail — including the structural basis of substrate recognition, cellular localization, or in vivo function of UNC — has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2017,\n      \"claim\": \"It was unknown whether PfMyoA required a dedicated chaperone to fold into an active motor; co-expression experiments showed the UCS-family chaperone UNC is necessary to produce functional PfMyoA, establishing UNC as the myosin-specific folding factor for the class XIV motor.\",\n      \"evidence\": \"Recombinant expression of full-length PfMyoA in a baculovirus/Sf9 system requiring co-expression of the UCS chaperone\",\n      \"pmids\": [\"28978649\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Single study, single lab, single method — chaperone requirement not independently reconstituted\",\n        \"Structural basis of UNC binding to and folding of PfMyoA not defined\",\n        \"In vivo requirement and localization of UNC in the parasite not tested\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How UNC recognizes and folds PfMyoA at the molecular level, and whether it is required for glideosome function and parasite motility in vivo, remains unresolved.\",\n      \"evidence\": \"No further discoveries in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No structural model of the UNC–PfMyoA interaction\",\n        \"No genetic loss-of-function or localization data in the parasite\",\n        \"No evidence on whether UNC chaperones other myosins\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0051082\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\"PfMyoA (class XIV myosin A)\"],\n    \"other_free_text\": [\n      \"UCS-family myosin-specific chaperone required for folding/production of functional PfMyoA [#0]\",\n      \"Demonstrated only via recombinant co-expression in a baculovirus/Sf9 system [#0]\"\n    ]\n  }\n}","audit_flag":{"gene":"PF3D7_1420200","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 1 of 1 cited papers name other catalogue genes (PF3D7_1017500, PF3D7_1246200, PF3D7_1246400). The summary may describe one of those genes."},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1420200","name":null,"product":"myosin-specific chaperone UNC, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1023000","name":null,"product":"myosin-specific chaperone UNC, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1337800","name":null,"product":"myosin-specific chaperone UNC, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"UNC","evaluation":null}