{"gene":"PF3D7_1403100","run_date":"2026-09-08T14:19:06","timeline":{"discoveries":[{"year":2020,"finding":"PF3D7_1403100 (CAPD2/condensin complex subunit 1) is a component of condensin I and condensin II complexes in Plasmodium falciparum, but these complexes form only in mature schizonts and during male gametogenesis; during early schizogony, the condensin core subunits SMC2/SMC4 localize to centromeres without forming condensin I or II complexes, indicating that CAPD2's complex membership is stage-dependent.","method":"Fluorescence microscopy, ChIP-seq, co-immunoprecipitation to define complex formation at different life-cycle stages","journal":"Cell Reports","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal complex identification by Co-IP and ChIP-seq in a single lab; CAPD2 inferred as condensin I subunit but mechanistic detail specific to CAPD2 itself is derived from the broader condensin complex study","pmids":["32049018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_1403100 (CAPD2) is a subunit of the condensin I complex in Plasmodium falciparum that associates with condensin core subunits SMC2/SMC4 in a stage-dependent manner — complex formation occurs in mature schizonts and during male gametogenesis but not in early schizogony, where SMC2/SMC4 localize to centromeres without forming canonical condensin I or II complexes."},"narrative":{"mechanistic_narrative":"PF3D7_1403100 (CAPD2) is a component of the condensin I and condensin II complexes in Plasmodium falciparum, where its association with the condensin core subunits SMC2/SMC4 is stage-dependent: canonical condensin complexes assemble only in mature schizonts and during male gametogenesis, whereas in early schizogony SMC2/SMC4 localize to centromeres without forming condensin I or II [PMID:32049018]. Beyond this stage-specific complex membership defined in [PMID:32049018], no further mechanistic detail specific to CAPD2 itself has been characterized in the available corpus.","teleology":[{"year":2020,"claim":"Whether CAPD2 participates in a canonical condensin complex in Plasmodium and when such a complex forms was unknown; defining its association with SMC2/SMC4 across life-cycle stages established that CAPD2's complex membership is temporally regulated rather than constitutive.","evidence":"Fluorescence microscopy, ChIP-seq, and co-immunoprecipitation defining condensin complex formation across life-cycle stages in P. falciparum","pmids":["32049018"],"confidence":"Medium","gaps":["Mechanistic detail specific to CAPD2 is inferred from the broader condensin complex study rather than from CAPD2-directed functional assays","The functional consequence of CAPD2 loss or the role of CAPD2 in chromosome condensation is not directly tested","How CAPD2 is recruited to or excluded from the SMC2/SMC4 core in a stage-dependent manner is not resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The direct molecular activity of CAPD2, its structural contribution to condensin, and the phenotypic requirement for CAPD2 in chromosome organization or gametogenesis remain uncharacterized.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No CAPD2-specific loss-of-function phenotype reported","No structural or biochemical characterization of CAPD2 within the condensin holocomplex","Determinants of stage-dependent complex assembly unknown"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["condensin I complex","condensin II complex"],"partners":["SMC2","SMC4"],"other_free_text":["CAPD2 association with SMC2/SMC4 is stage-dependent, occurring in mature schizonts and during male gametogenesis but not in early schizogony [#0]","During early schizogony SMC2/SMC4 localize to centromeres without forming condensin I or II complexes [#0]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"32049018","id":"PMC_32049018","title":"Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission.","date":"2020","source":"Cell reports","url":"https://pubmed.ncbi.nlm.nih.gov/32049018","citation_count":30,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3319,"output_tokens":452,"usd":0.008368,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4957,"output_tokens":1078,"usd":0.025868,"stage2_stop_reason":"end_turn"},"total_usd":0.034236,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2020,\n      \"finding\": \"PF3D7_1403100 (CAPD2/condensin complex subunit 1) is a component of condensin I and condensin II complexes in Plasmodium falciparum, but these complexes form only in mature schizonts and during male gametogenesis; during early schizogony, the condensin core subunits SMC2/SMC4 localize to centromeres without forming condensin I or II complexes, indicating that CAPD2's complex membership is stage-dependent.\",\n      \"method\": \"Fluorescence microscopy, ChIP-seq, co-immunoprecipitation to define complex formation at different life-cycle stages\",\n      \"journal\": \"Cell Reports\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal complex identification by Co-IP and ChIP-seq in a single lab; CAPD2 inferred as condensin I subunit but mechanistic detail specific to CAPD2 itself is derived from the broader condensin complex study\",\n      \"pmids\": [\"32049018\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_1403100 (CAPD2) is a subunit of the condensin I complex in Plasmodium falciparum that associates with condensin core subunits SMC2/SMC4 in a stage-dependent manner — complex formation occurs in mature schizonts and during male gametogenesis but not in early schizogony, where SMC2/SMC4 localize to centromeres without forming canonical condensin I or II complexes.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1403100 (CAPD2) is a component of the condensin I and condensin II complexes in Plasmodium falciparum, where its association with the condensin core subunits SMC2/SMC4 is stage-dependent: canonical condensin complexes assemble only in mature schizonts and during male gametogenesis, whereas in early schizogony SMC2/SMC4 localize to centromeres without forming condensin I or II [#0]. Beyond this stage-specific complex membership defined in [#0], no further mechanistic detail specific to CAPD2 itself has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2020,\n      \"claim\": \"Whether CAPD2 participates in a canonical condensin complex in Plasmodium and when such a complex forms was unknown; defining its association with SMC2/SMC4 across life-cycle stages established that CAPD2's complex membership is temporally regulated rather than constitutive.\",\n      \"evidence\": \"Fluorescence microscopy, ChIP-seq, and co-immunoprecipitation defining condensin complex formation across life-cycle stages in P. falciparum\",\n      \"pmids\": [\"32049018\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Mechanistic detail specific to CAPD2 is inferred from the broader condensin complex study rather than from CAPD2-directed functional assays\",\n        \"The functional consequence of CAPD2 loss or the role of CAPD2 in chromosome condensation is not directly tested\",\n        \"How CAPD2 is recruited to or excluded from the SMC2/SMC4 core in a stage-dependent manner is not resolved\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct molecular activity of CAPD2, its structural contribution to condensin, and the phenotypic requirement for CAPD2 in chromosome organization or gametogenesis remain uncharacterized.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No CAPD2-specific loss-of-function phenotype reported\",\n        \"No structural or biochemical characterization of CAPD2 within the condensin holocomplex\",\n        \"Determinants of stage-dependent complex assembly unknown\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [\"condensin I complex\", \"condensin II complex\"],\n    \"partners\": [\"SMC2\", \"SMC4\"],\n    \"other_free_text\": [\n      \"CAPD2 association with SMC2/SMC4 is stage-dependent, occurring in mature schizonts and during male gametogenesis but not in early schizogony [#0]\",\n      \"During early schizogony SMC2/SMC4 localize to centromeres without forming condensin I or II complexes [#0]\"\n    ]\n  }\n}","audit_flag":{"gene":"PF3D7_1403100","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 1 of 1 cited papers name other catalogue genes (PF3D7_0509100, PF3D7_0616200, PF3D7_1318400). The summary may describe one of those genes."},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1403100","name":null,"product":"condensin complex subunit 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1038900","name":null,"product":"condensin complex subunit 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1355000","name":null,"product":"condensin complex subunit 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"CAPD2","evaluation":null}