{"gene":"PF3D7_1351400","run_date":"2026-09-08T14:19:06","timeline":{"discoveries":[{"year":2014,"finding":"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution established that PF3D7_1351400 (60S ribosomal protein L17) is a structural component of the large (60S) ribosomal subunit of the cytoplasmic 80S ribosome.","method":"Cryo-EM structure determination at 3.2 Å resolution","journal":"eLife","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — high-resolution cryo-EM structure of the intact ribosome, but no functional experiments or mutagenesis specific to L17 itself; membership in the complex is inferred from the structural map","pmids":["24913268"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"finding":"In situ cryo-electron tomography of P. falciparum-infected erythrocytes resolved eight high-resolution structures of the native malarial ribosome in actively translating states, confirming that PF3D7_1351400 (L17) is a constituent of the 60S subunit within the native cellular translation machinery.","method":"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes","journal":"Nature structural & molecular biology","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — state-of-the-art in situ structural method, but no functional experiments or mutagenesis specific to L17; structural membership in the complex is the only finding attributable to this gene","pmids":["40825879"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_1351400 (L17) is established as a structural component of the 60S large subunit of the P. falciparum cytoplasmic 80S ribosome, as visualised by cryo-EM and in situ cryo-electron tomography; no specific enzymatic activity, binding partners, post-translational modifications, or functional role beyond ribosome membership have been mechanistically defined for this protein."},"narrative":{"mechanistic_narrative":"PF3D7_1351400 (L17) is a structural component of the large (60S) subunit of the Plasmodium falciparum cytoplasmic 80S ribosome, as established by cryo-EM of the intact ribosome [PMID:24913268] and confirmed within the native, actively translating machinery by in situ cryo-electron tomography inside infected erythrocytes [PMID:40825879]. Beyond its membership in the 60S subunit, no enzymatic activity, binding partners, post-translational modifications, or functional role specific to L17 has been characterized in the available corpus.","teleology":[{"year":2014,"claim":"Whether PF3D7_1351400 is part of the parasite's ribosome was resolved by placing it as a structural constituent of the 60S subunit within the assembled 80S ribosome.","evidence":"Cryo-EM structure determination of the P. falciparum 80S ribosome at 3.2 Å resolution","pmids":["24913268"],"confidence":"Medium","gaps":["Membership is inferred from the structural map; no mutagenesis or functional assay specific to L17","No enzymatic activity or binding partner beyond the ribosome defined","Role in ribosome assembly or translation not tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"claim":"Whether L17's ribosomal placement holds in the native cellular context, rather than only in purified particles, was addressed by resolving the ribosome in actively translating states inside intact parasites.","evidence":"In situ cryo-electron tomography of the native ribosome inside P. falciparum-infected human erythrocytes, yielding eight translating-state structures","pmids":["40825879"],"confidence":"Medium","gaps":["Structural membership is the only finding attributable to L17; no functional role assigned","No evidence for regulation, modification, or extra-ribosomal moonlighting function","Contribution of L17 to specific translational states not dissected"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"What functional or regulatory role L17 plays beyond being a structural subunit of the 60S ribosome remains unknown.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No gene-specific mutagenesis, knockdown, or depletion phenotype reported","No binding partners outside the ribosome, no post-translational modifications identified","No test of essentiality or druggability specific to L17"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["60S large ribosomal subunit of the P. falciparum cytoplasmic 80S ribosome"],"partners":[],"other_free_text":["L17 is attributable only as a structural constituent of the 60S subunit; the corpus establishes nothing about this gene beyond its membership in the ribosome.","Evidence rests entirely on structural methods (cryo-EM [#0] and in situ cryo-electron tomography [#1]) with no functional or mutagenesis data specific to L17."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"24913268","id":"PMC_24913268","title":"Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.","date":"2014","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/24913268","citation_count":272,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"28288098","id":"PMC_28288098","title":"Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis.","date":"2017","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/28288098","citation_count":136,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"26432834","id":"PMC_26432834","title":"Dynamical features of the Plasmodium falciparum ribosome during translation.","date":"2015","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/26432834","citation_count":45,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40825879","id":"PMC_40825879","title":"Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug.","date":"2025","source":"Nature structural & molecular biology","url":"https://pubmed.ncbi.nlm.nih.gov/40825879","citation_count":7,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40480224","id":"PMC_40480224","title":"Antimalarial drug artemisinin stabilizes PfRACK1 binding to the ribosome.","date":"2025","source":"Structure (London, England : 1993)","url":"https://pubmed.ncbi.nlm.nih.gov/40480224","citation_count":3,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":4837,"output_tokens":634,"usd":0.012011,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5208,"output_tokens":1394,"usd":0.030445,"stage2_stop_reason":"end_turn"},"total_usd":0.042456,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2014,\n      \"finding\": \"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution established that PF3D7_1351400 (60S ribosomal protein L17) is a structural component of the large (60S) ribosomal subunit of the cytoplasmic 80S ribosome.\",\n      \"method\": \"Cryo-EM structure determination at 3.2 Å resolution\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — high-resolution cryo-EM structure of the intact ribosome, but no functional experiments or mutagenesis specific to L17 itself; membership in the complex is inferred from the structural map\",\n      \"pmids\": [\"24913268\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"In situ cryo-electron tomography of P. falciparum-infected erythrocytes resolved eight high-resolution structures of the native malarial ribosome in actively translating states, confirming that PF3D7_1351400 (L17) is a constituent of the 60S subunit within the native cellular translation machinery.\",\n      \"method\": \"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes\",\n      \"journal\": \"Nature structural & molecular biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — state-of-the-art in situ structural method, but no functional experiments or mutagenesis specific to L17; structural membership in the complex is the only finding attributable to this gene\",\n      \"pmids\": [\"40825879\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_1351400 (L17) is established as a structural component of the 60S large subunit of the P. falciparum cytoplasmic 80S ribosome, as visualised by cryo-EM and in situ cryo-electron tomography; no specific enzymatic activity, binding partners, post-translational modifications, or functional role beyond ribosome membership have been mechanistically defined for this protein.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1351400 (L17) is a structural component of the large (60S) subunit of the Plasmodium falciparum cytoplasmic 80S ribosome, as established by cryo-EM of the intact ribosome [#0] and confirmed within the native, actively translating machinery by in situ cryo-electron tomography inside infected erythrocytes [#1]. Beyond its membership in the 60S subunit, no enzymatic activity, binding partners, post-translational modifications, or functional role specific to L17 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2014,\n      \"claim\": \"Whether PF3D7_1351400 is part of the parasite's ribosome was resolved by placing it as a structural constituent of the 60S subunit within the assembled 80S ribosome.\",\n      \"evidence\": \"Cryo-EM structure determination of the P. falciparum 80S ribosome at 3.2 Å resolution\",\n      \"pmids\": [\"24913268\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Membership is inferred from the structural map; no mutagenesis or functional assay specific to L17\",\n        \"No enzymatic activity or binding partner beyond the ribosome defined\",\n        \"Role in ribosome assembly or translation not tested\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Whether L17's ribosomal placement holds in the native cellular context, rather than only in purified particles, was addressed by resolving the ribosome in actively translating states inside intact parasites.\",\n      \"evidence\": \"In situ cryo-electron tomography of the native ribosome inside P. falciparum-infected human erythrocytes, yielding eight translating-state structures\",\n      \"pmids\": [\"40825879\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Structural membership is the only finding attributable to L17; no functional role assigned\",\n        \"No evidence for regulation, modification, or extra-ribosomal moonlighting function\",\n        \"Contribution of L17 to specific translational states not dissected\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"What functional or regulatory role L17 plays beyond being a structural subunit of the 60S ribosome remains unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No gene-specific mutagenesis, knockdown, or depletion phenotype reported\",\n        \"No binding partners outside the ribosome, no post-translational modifications identified\",\n        \"No test of essentiality or druggability specific to L17\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003735\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0022625\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0005737\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006412\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"complexes\": [\n      \"60S large ribosomal subunit of the P. falciparum cytoplasmic 80S ribosome\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"L17 is attributable only as a structural constituent of the 60S subunit; the corpus establishes nothing about this gene beyond its membership in the ribosome.\",\n      \"Evidence rests entirely on structural methods (cryo-EM [#0] and in situ cryo-electron tomography [#1]) with no functional or mutagenesis data specific to L17.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1351400","name":null,"product":"60S ribosomal protein L17, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1364200","name":null,"product":"60S ribosomal protein L17, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1249500","name":null,"product":"60S ribosomal protein L17, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"","evaluation":null}