{"gene":"PF3D7_1329100","run_date":"2026-09-08T14:19:06","timeline":{"discoveries":[{"year":2026,"finding":"MyoF (PF3D7_1329100) is a plus-end-directed, processive motor with a long neck region capable of binding up to six copies of the calmodulin homologue PfCaM, as determined by biochemical assays and structural prediction.","method":"Biochemical motility assays, structural prediction, calmodulin-binding assays","journal":"Communications biology","confidence":"Medium","confidence_rationale":"Tier 1-2 / Weak — in vitro biochemical assays establishing motor directionality and processivity, single lab, limited replication","pmids":["42141018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"finding":"The MyoF (PF3D7_1329100) tail contains predicted WD40 and Rab-like domains not identified in any other eukaryotic myosin class; pull-down experiments identified interactions between MyoF and trafficking proteins including the vesicle marker PfRab18.","method":"Structural prediction (AlphaFold), pull-down assays, mass spectrometry","journal":"Communications biology","confidence":"Medium","confidence_rationale":"Tier 2-3 / Weak — pull-down with interactor identification, structural domain prediction, single lab","pmids":["42141018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"finding":"MyoF (PF3D7_1329100) localises to a perinuclear membrane compartment, as shown by expansion microscopy and immunoelectron microscopy.","method":"Expansion microscopy, immunoelectron microscopy","journal":"Communications biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — direct localization by two orthogonal imaging methods, single lab","pmids":["42141018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"finding":"Transient knockdown of MyoF (PF3D7_1329100) using the glmS ribozyme system impairs parasite growth during asexual blood stages, indicating a role in asexual replication.","method":"glmS ribozyme conditional knockdown, growth assay","journal":"Communications biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — conditional knockdown with defined growth phenotype, single lab, single method","pmids":["42141018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2019,"finding":"MyoF (PF3D7_1329100) is likely essential in asexual blood stages, as gene deletion attempts were unsuccessful, suggesting an essential function in this life cycle stage.","method":"Gene deletion attempt (failed, implying essentiality)","journal":"Cellular microbiology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — essentiality inferred from failed gene deletion, single lab, no conditional knockdown confirmation in this study","pmids":["31283102"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"finding":"MyoF (PF3D7_1329100) is found at the Kelch13 (K13) compartment in P. falciparum asexual blood stages, as determined by BioID proximity labelling and co-localisation analyses.","method":"BioID proximity labelling, fluorescence microscopy co-localisation","journal":"PLoS pathogens","confidence":"Low","confidence_rationale":"Tier 3 / Weak — localization by BioID and microscopy, no direct functional dissection of MyoF at this compartment, single study","pmids":["38039338"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2016,"finding":"In Toxoplasma gondii, the armadillo repeats only protein (ARO) interacts with myosin F (MyoF) at rhoptries; structural analysis identified a conserved patch of aromatic and acidic residues on ARO as the putative MyoF-binding site. Plasmodium falciparum ARO functionally complements ARO depletion in T. gondii and interacts with the same partners, indicating conservation of this interaction in P. falciparum MyoF.","method":"Co-immunoprecipitation, structural analysis (crystallography), complementation assay","journal":"Journal of cell science","confidence":"Low","confidence_rationale":"Tier 2-3 / Weak — direct interaction mapped structurally in T. gondii, P. falciparum MyoF involvement inferred by conservation and complementation, not directly demonstrated in P. falciparum","pmids":["26769898"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2005,"finding":"MyoF (PF3D7_1329100) mRNA increases steadily throughout asexual blood stage development, and the gene encodes two consensus IQ light chain-binding motifs, as identified by RT-PCR and sequence analysis.","method":"RT-PCR, sequence/phylogenetic analysis","journal":"Cell motility and the cytoskeleton","confidence":"Low","confidence_rationale":"Tier 3 / Weak — transcriptional profiling and sequence analysis only, no protein-level functional assay, single lab","pmids":["15754360"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"MyoF (PF3D7_1329100) is a class XXII, plus-end-directed, processive myosin motor that localises to a perinuclear membrane compartment in P. falciparum asexual blood stages; its long neck region binds up to six copies of PfCaM, its tail contains unique Rab-like and WD40 domains, it interacts with vesicle-trafficking proteins including PfRab18, it is found at the K13 endocytic compartment, and it is likely essential for asexual replication, with a conserved role in rhoptry-associated ARO interactions implicated by cross-species complementation data."},"narrative":{"mechanistic_narrative":"MyoF (PF3D7_1329100) is a myosin motor of Plasmodium falciparum that supports asexual blood-stage replication through a role in intracellular membrane trafficking [PMID:42141018]. Biochemical and structural analyses establish it as a plus-end-directed, processive motor whose long neck region binds up to six copies of the calmodulin homologue PfCaM, consistent with an early observation that the gene encodes IQ light-chain-binding motifs [PMID:42141018, PMID:15754360]. Its tail carries WD40 and Rab-like domains not found in other eukaryotic myosin classes, and it associates with vesicle-trafficking proteins including the vesicle marker PfRab18 [PMID:42141018]. MyoF localises to a perinuclear membrane compartment and is present at the Kelch13 endocytic compartment [PMID:42141018, PMID:38039338]. Conditional glmS knockdown impairs asexual growth, and gene deletion attempts have been unsuccessful, together indicating an essential function during the asexual cycle [PMID:42141018, PMID:31283102]. Beyond these findings, the substrates transported by MyoF and the mechanism by which its motor and trafficking domains are coordinated have not been characterized in the available corpus.","teleology":[{"year":2005,"claim":"Before any functional characterization, it was unknown whether PF3D7_1329100 was expressed during and structured for a role in blood-stage biology; transcript profiling and sequence analysis placed it as a blood-stage myosin with light-chain-binding capacity.","evidence":"RT-PCR expression profiling and sequence/phylogenetic analysis in P. falciparum","pmids":["15754360"],"confidence":"Low","gaps":["No protein-level or functional assay","IQ motif light-chain identity not established"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2016,"claim":"It was unclear whether MyoF participates in a conserved apicomplexan interaction network; structural and complementation work in Toxoplasma gondii implicated a conserved ARO-binding interaction that likely extends to P. falciparum MyoF.","evidence":"Co-immunoprecipitation, crystallography, and cross-species complementation in T. gondii with P. falciparum ARO","pmids":["26769898"],"confidence":"Low","gaps":["P. falciparum MyoF-ARO interaction inferred by conservation, not directly demonstrated","Rhoptry association of P. falciparum MyoF not shown directly"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2019,"claim":"Whether MyoF is required for parasite survival was unknown; failed gene deletion attempts pointed to an essential asexual-stage function.","evidence":"Attempted gene knockout in P. falciparum asexual blood stages","pmids":["31283102"],"confidence":"Low","gaps":["Essentiality inferred from failed deletion, not confirmed by conditional depletion in this study","Stage-specific requirement not defined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"claim":"The subcellular pathway MyoF acts in was undefined; proximity labelling placed it at the Kelch13 endocytic compartment, linking it to hemoglobin uptake machinery.","evidence":"BioID proximity labelling and fluorescence co-localisation in P. falciparum","pmids":["38039338"],"confidence":"Low","gaps":["No functional dissection of MyoF at the K13 compartment","Contribution to endocytosis not tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"claim":"The motor properties, domain architecture, localization, and essentiality of MyoF were not directly established; combined biochemical, structural, imaging, and conditional-knockdown work defined it as a plus-end-directed processive PfCaM-binding motor with unique trafficking domains at a perinuclear compartment required for asexual growth.","evidence":"Motility and calmodulin-binding assays, AlphaFold prediction, pull-down/MS, expansion and immunoelectron microscopy, and glmS knockdown in P. falciparum","pmids":["42141018"],"confidence":"Medium","gaps":["Cargo transported by MyoF not identified","Functional role of WD40 and Rab-like tail domains untested","PfRab18 interaction not validated reciprocally or functionally","Coordination of motor activity with trafficking function unresolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"It remains unknown what cargo MyoF transports and how its motor activity, PfCaM regulation, and unique tail domains are integrated to drive the trafficking events essential for asexual replication.","evidence":"","pmids":[],"confidence":"Low","gaps":["No identified transport substrate","Mechanistic link between K13-compartment localization and endocytosis untested","No structure of the full-length motor-tail complex"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["PFCAM","PFRAB18","ARO (VIA CROSS-SPECIES CONSERVATION)"],"other_free_text":["Plus-end-directed, processive myosin motor with a long neck binding up to six PfCaM copies [#0].","Tail contains WD40 and Rab-like domains not found in other eukaryotic myosin classes [#1].","Localises to a perinuclear membrane compartment and to the Kelch13 endocytic compartment [#2, #5].","Likely essential in asexual blood stages; glmS knockdown impairs growth and deletion attempts failed [#3, #4].","Encodes two IQ light-chain-binding motifs and transcript rises through asexual development [#7]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"31283102","id":"PMC_31283102","title":"Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages.","date":"2019","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/31283102","citation_count":29,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["MyoF"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"26769898","id":"PMC_26769898","title":"Structural and functional dissection of Toxoplasma gondii armadillo repeats only protein.","date":"2016","source":"Journal of cell science","url":"https://pubmed.ncbi.nlm.nih.gov/26769898","citation_count":27,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["MyoF","myosin F"],"competing_organism_flag":true,"competing_organism_match":"Toxoplasma","competing_organism_only":true},{"pmid":"38039338","id":"PMC_38039338","title":"The Kelch13 compartment contains highly divergent vesicle trafficking proteins in malaria parasites.","date":"2023","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/38039338","citation_count":21,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["MyoF"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"15754360","id":"PMC_15754360","title":"Plasmodium falciparum myosins: transcription and translation during asexual parasite development.","date":"2005","source":"Cell motility and the cytoskeleton","url":"https://pubmed.ncbi.nlm.nih.gov/15754360","citation_count":17,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfMyoF"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42141018","id":"PMC_42141018","title":"Plasmodium falciparum PfMyoF contains a Rab-like tail domain and associates with perinuclear membrane trafficking proteins.","date":"2026","source":"Communications biology","url":"https://pubmed.ncbi.nlm.nih.gov/42141018","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfMyoF"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":5372,"output_tokens":1866,"usd":0.022053,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6990,"output_tokens":2453,"usd":0.048137,"stage2_stop_reason":"end_turn"},"total_usd":0.07019,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2026,\n      \"finding\": \"MyoF (PF3D7_1329100) is a plus-end-directed, processive motor with a long neck region capable of binding up to six copies of the calmodulin homologue PfCaM, as determined by biochemical assays and structural prediction.\",\n      \"method\": \"Biochemical motility assays, structural prediction, calmodulin-binding assays\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1-2 / Weak — in vitro biochemical assays establishing motor directionality and processivity, single lab, limited replication\",\n      \"pmids\": [\"42141018\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"The MyoF (PF3D7_1329100) tail contains predicted WD40 and Rab-like domains not identified in any other eukaryotic myosin class; pull-down experiments identified interactions between MyoF and trafficking proteins including the vesicle marker PfRab18.\",\n      \"method\": \"Structural prediction (AlphaFold), pull-down assays, mass spectrometry\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2-3 / Weak — pull-down with interactor identification, structural domain prediction, single lab\",\n      \"pmids\": [\"42141018\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"MyoF (PF3D7_1329100) localises to a perinuclear membrane compartment, as shown by expansion microscopy and immunoelectron microscopy.\",\n      \"method\": \"Expansion microscopy, immunoelectron microscopy\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — direct localization by two orthogonal imaging methods, single lab\",\n      \"pmids\": [\"42141018\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"Transient knockdown of MyoF (PF3D7_1329100) using the glmS ribozyme system impairs parasite growth during asexual blood stages, indicating a role in asexual replication.\",\n      \"method\": \"glmS ribozyme conditional knockdown, growth assay\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — conditional knockdown with defined growth phenotype, single lab, single method\",\n      \"pmids\": [\"42141018\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"MyoF (PF3D7_1329100) is likely essential in asexual blood stages, as gene deletion attempts were unsuccessful, suggesting an essential function in this life cycle stage.\",\n      \"method\": \"Gene deletion attempt (failed, implying essentiality)\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — essentiality inferred from failed gene deletion, single lab, no conditional knockdown confirmation in this study\",\n      \"pmids\": [\"31283102\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"MyoF (PF3D7_1329100) is found at the Kelch13 (K13) compartment in P. falciparum asexual blood stages, as determined by BioID proximity labelling and co-localisation analyses.\",\n      \"method\": \"BioID proximity labelling, fluorescence microscopy co-localisation\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — localization by BioID and microscopy, no direct functional dissection of MyoF at this compartment, single study\",\n      \"pmids\": [\"38039338\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"In Toxoplasma gondii, the armadillo repeats only protein (ARO) interacts with myosin F (MyoF) at rhoptries; structural analysis identified a conserved patch of aromatic and acidic residues on ARO as the putative MyoF-binding site. Plasmodium falciparum ARO functionally complements ARO depletion in T. gondii and interacts with the same partners, indicating conservation of this interaction in P. falciparum MyoF.\",\n      \"method\": \"Co-immunoprecipitation, structural analysis (crystallography), complementation assay\",\n      \"journal\": \"Journal of cell science\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 2-3 / Weak — direct interaction mapped structurally in T. gondii, P. falciparum MyoF involvement inferred by conservation and complementation, not directly demonstrated in P. falciparum\",\n      \"pmids\": [\"26769898\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2005,\n      \"finding\": \"MyoF (PF3D7_1329100) mRNA increases steadily throughout asexual blood stage development, and the gene encodes two consensus IQ light chain-binding motifs, as identified by RT-PCR and sequence analysis.\",\n      \"method\": \"RT-PCR, sequence/phylogenetic analysis\",\n      \"journal\": \"Cell motility and the cytoskeleton\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — transcriptional profiling and sequence analysis only, no protein-level functional assay, single lab\",\n      \"pmids\": [\"15754360\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"MyoF (PF3D7_1329100) is a class XXII, plus-end-directed, processive myosin motor that localises to a perinuclear membrane compartment in P. falciparum asexual blood stages; its long neck region binds up to six copies of PfCaM, its tail contains unique Rab-like and WD40 domains, it interacts with vesicle-trafficking proteins including PfRab18, it is found at the K13 endocytic compartment, and it is likely essential for asexual replication, with a conserved role in rhoptry-associated ARO interactions implicated by cross-species complementation data.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"MyoF (PF3D7_1329100) is a myosin motor of Plasmodium falciparum that supports asexual blood-stage replication through a role in intracellular membrane trafficking [#0, #3]. Biochemical and structural analyses establish it as a plus-end-directed, processive motor whose long neck region binds up to six copies of the calmodulin homologue PfCaM, consistent with an early observation that the gene encodes IQ light-chain-binding motifs [#0, #7]. Its tail carries WD40 and Rab-like domains not found in other eukaryotic myosin classes, and it associates with vesicle-trafficking proteins including the vesicle marker PfRab18 [#1]. MyoF localises to a perinuclear membrane compartment and is present at the Kelch13 endocytic compartment [#2, #5]. Conditional glmS knockdown impairs asexual growth, and gene deletion attempts have been unsuccessful, together indicating an essential function during the asexual cycle [#3, #4]. Beyond these findings, the substrates transported by MyoF and the mechanism by which its motor and trafficking domains are coordinated have not been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2005,\n      \"claim\": \"Before any functional characterization, it was unknown whether PF3D7_1329100 was expressed during and structured for a role in blood-stage biology; transcript profiling and sequence analysis placed it as a blood-stage myosin with light-chain-binding capacity.\",\n      \"evidence\": \"RT-PCR expression profiling and sequence/phylogenetic analysis in P. falciparum\",\n      \"pmids\": [\"15754360\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No protein-level or functional assay\", \"IQ motif light-chain identity not established\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"It was unclear whether MyoF participates in a conserved apicomplexan interaction network; structural and complementation work in Toxoplasma gondii implicated a conserved ARO-binding interaction that likely extends to P. falciparum MyoF.\",\n      \"evidence\": \"Co-immunoprecipitation, crystallography, and cross-species complementation in T. gondii with P. falciparum ARO\",\n      \"pmids\": [\"26769898\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"P. falciparum MyoF-ARO interaction inferred by conservation, not directly demonstrated\", \"Rhoptry association of P. falciparum MyoF not shown directly\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2019,\n      \"claim\": \"Whether MyoF is required for parasite survival was unknown; failed gene deletion attempts pointed to an essential asexual-stage function.\",\n      \"evidence\": \"Attempted gene knockout in P. falciparum asexual blood stages\",\n      \"pmids\": [\"31283102\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"Essentiality inferred from failed deletion, not confirmed by conditional depletion in this study\", \"Stage-specific requirement not defined\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"The subcellular pathway MyoF acts in was undefined; proximity labelling placed it at the Kelch13 endocytic compartment, linking it to hemoglobin uptake machinery.\",\n      \"evidence\": \"BioID proximity labelling and fluorescence co-localisation in P. falciparum\",\n      \"pmids\": [\"38039338\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No functional dissection of MyoF at the K13 compartment\", \"Contribution to endocytosis not tested\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"The motor properties, domain architecture, localization, and essentiality of MyoF were not directly established; combined biochemical, structural, imaging, and conditional-knockdown work defined it as a plus-end-directed processive PfCaM-binding motor with unique trafficking domains at a perinuclear compartment required for asexual growth.\",\n      \"evidence\": \"Motility and calmodulin-binding assays, AlphaFold prediction, pull-down/MS, expansion and immunoelectron microscopy, and glmS knockdown in P. falciparum\",\n      \"pmids\": [\"42141018\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Cargo transported by MyoF not identified\", \"Functional role of WD40 and Rab-like tail domains untested\", \"PfRab18 interaction not validated reciprocally or functionally\", \"Coordination of motor activity with trafficking function unresolved\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown what cargo MyoF transports and how its motor activity, PfCaM regulation, and unique tail domains are integrated to drive the trafficking events essential for asexual replication.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No identified transport substrate\", \"Mechanistic link between K13-compartment localization and endocytosis untested\", \"No structure of the full-length motor-tail complex\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0000146\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0003774\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0005516\", \"supporting_discovery_ids\": [0, 7]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0031090\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0016192\", \"supporting_discovery_ids\": [1]},\n      {\"term_id\": \"GO:0006897\", \"supporting_discovery_ids\": [5]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\"PfCaM\", \"PfRab18\", \"ARO (via cross-species conservation)\"],\n    \"other_free_text\": [\n      \"Plus-end-directed, processive myosin motor with a long neck binding up to six PfCaM copies [#0].\",\n      \"Tail contains WD40 and Rab-like domains not found in other eukaryotic myosin classes [#1].\",\n      \"Localises to a perinuclear membrane compartment and to the Kelch13 endocytic compartment [#2, #5].\",\n      \"Likely essential in asexual blood stages; glmS knockdown impairs growth and deletion attempts failed [#3, #4].\",\n      \"Encodes two IQ light-chain-binding motifs and transcript rises through asexual development [#7].\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1329100","name":null,"product":"myosin F, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1344100","name":null,"product":"myosin F","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1271300","name":null,"product":"myosin F, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"MyoF","evaluation":null}