{"gene":"PF3D7_1318400","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2020,"finding":"SMC2 (PF3D7_1318400) and SMC4 localize to a distinct centromeric focus during early schizogony (confirmed by NDC80 fluorescence and ChIP-seq), but do not form condensin I or II complexes at this stage. In mature schizonts and during male gametogenesis, SMC2/SMC4 show diffuse chromosomal/nuclear distribution and form both condensin I and condensin II complexes. Knockdown of smc2 reveals an essential role in parasite proliferation (asexual) and transmission (male gametogenesis/exflagellation).","method":"Fluorescence live-cell imaging, ChIP-seq (centromere localization), conditional gene knockdown, co-immunoprecipitation (condensin I/II complex formation)","journal":"Cell reports","confidence":"High","confidence_rationale":"Tier 2 / Strong — multiple orthogonal methods (ChIP-seq, live imaging, Co-IP, conditional knockdown with defined phenotype) in a single focused study on this gene","pmids":["32049018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2024,"finding":"SMC2 (PF3D7_1318400) is a component of the condensin I complex in P. falciparum. Knockout of the condensin I kleisin subunit PfCAP-H does not alter SMC2/SMC4 expression but disrupts their localization at mitotic chromosomes, indicating that PfCAP-H is required for proper loading of the condensin I complex (including SMC2) onto chromosomes during asexual blood-stage division.","method":"Inducible DiCre knockout of PfCAP-H, immunofluorescence assay (IFA) for condensin complex localization, immunoblot","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean inducible KO with IFA-based localization readout for SMC2, single lab, two orthogonal methods","pmids":["38564676"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":"3D7"}]},{"year":2025,"finding":"SMC2 (PF3D7_1318400) and SMC4 co-precipitate with the kinase MAP2-GFP during male gametogenesis in P. berghei, suggesting that SMC2/SMC4 (condensin) interact with MAP2 and may be regulated by this kinase during atypical chromosome condensation associated with the MTOC.","method":"Immunoprecipitation/mass spectrometry (GFP pulldown of MAP2) from activated male gametocytes","journal":"Life science alliance","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single Co-IP/MS experiment, no reciprocal pulldown or direct functional validation of SMC2 interaction with MAP2","pmids":["40127922"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2023,"finding":"SMC2 (PF3D7_1318400) and SMC4 (condensin) are enriched in immunoprecipitates of EB1-GFP from P. berghei gametocytes, placing SMC2 in a protein interaction network at the spindle-kinetochore interface during sexual-stage endomitosis.","method":"Immunoprecipitation/mass spectrometry (GFP pulldown of EB1) from activated gametocytes, PCA of peptide spectral counts","journal":"Nature communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single Co-IP/MS experiment identifying SMC2 as an interactor, no direct functional follow-up on SMC2 itself","pmids":["37704606"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"SMC2 (PF3D7_1318400) is a core condensin subunit in Plasmodium that localizes to centromeres (without forming condensin I or II complexes) during early schizogony, then redistributes diffusely across chromosomes in mature schizonts and male gametocytes where it assembles into both condensin I and condensin II complexes; knockdown of SMC2 is essential for asexual parasite proliferation and male gametogenesis/transmission, and its proper chromosomal loading depends on the condensin I kleisin subunit PfCAP-H."},"narrative":{"mechanistic_narrative":"SMC2 (PF3D7_1318400) is a core structural maintenance of chromosomes protein that serves as a condensin subunit governing chromosome organization during both asexual proliferation and male gametogenesis in Plasmodium [PMID:32049018]. During early schizogony SMC2 (together with SMC4) concentrates at a discrete centromeric focus without assembling into condensin I or II complexes, then redistributes diffusely across chromosomes in mature schizonts and activated male gametocytes, where it assembles into both condensin I and condensin II complexes [PMID:32049018]. Conditional knockdown establishes that SMC2 is essential for asexual parasite proliferation and for male gametogenesis/exflagellation, linking it to parasite transmission [PMID:32049018]. In P. falciparum, proper chromosomal loading of the condensin I-associated SMC2 depends on the kleisin subunit PfCAP-H, whose knockout disrupts SMC2/SMC4 localization at mitotic chromosomes without altering their expression [PMID:38564676]. Beyond these findings, the biochemical activity of SMC2 itself has not been characterized in the available corpus.","teleology":[{"year":2020,"claim":"Established that SMC2 is a stage-regulated condensin subunit whose localization and complex membership change through the cell cycle, and that it is functionally essential for proliferation and transmission.","evidence":"ChIP-seq and live imaging for localization, Co-IP for condensin I/II assembly, and conditional knockdown with defined phenotypes in P. falciparum and P. berghei","pmids":["32049018"],"confidence":"High","gaps":["Molecular basis for the centromere-to-diffuse redistribution is unresolved","The trigger for condensin I versus condensin II assembly is not defined","No direct enzymatic or DNA-binding activity of SMC2 demonstrated"],"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2023,"claim":"Placed SMC2 within a spindle-kinetochore interaction network during sexual-stage endomitosis, hinting at coordination with the mitotic apparatus.","evidence":"EB1-GFP immunoprecipitation/mass spectrometry from activated P. berghei gametocytes","pmids":["37704606"],"confidence":"Low","gaps":["Single Co-IP/MS without reciprocal pulldown or functional follow-up on SMC2","Whether the association is direct or bridged by other condensin subunits is unknown"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2024,"claim":"Identified the condensin I kleisin PfCAP-H as required for loading SMC2 onto mitotic chromosomes, distinguishing complex assembly from chromosomal recruitment.","evidence":"Inducible DiCre knockout of PfCAP-H with IFA-based SMC2/SMC4 localization and immunoblot in P. falciparum 3D7","pmids":["38564676"],"confidence":"Medium","gaps":["Whether SMC2 also requires distinct factors for condensin II loading is untested","Direct SMC2–PfCAP-H binding interface not defined"],"attribution":[{"species":"Plasmodium falciparum","strain":"3D7"}]},{"year":2025,"claim":"Linked SMC2 to a mitotic kinase, raising the possibility that condensin is regulated during atypical male-gametogenesis chromosome condensation.","evidence":"MAP2-GFP immunoprecipitation/mass spectrometry from activated male gametocytes in P. berghei","pmids":["40127922"],"confidence":"Low","gaps":["Single Co-IP/MS without reciprocal pulldown or direct functional validation of an SMC2–MAP2 interaction","No evidence that MAP2 phosphorylates or directly regulates SMC2"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The intrinsic biochemical activity of SMC2 and the signals driving its stage-specific centromeric-to-diffuse redistribution and condensin I/II assembly remain undefined.","evidence":"","pmids":[],"confidence":"Low","gaps":["No structural or ATPase/DNA-engagement data for SMC2 in this corpus","Mechanism switching SMC2 between condensin I and condensin II unresolved","Regulatory kinase inputs to SMC2 not functionally established"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["condensin I complex","condensin II complex"],"partners":["SMC4","PFCAP-H (CONDENSIN I KLEISIN)","MAP2 KINASE (P. BERGHEI, CO-IP/MS)","EB1 (P. BERGHEI, CO-IP/MS)"],"other_free_text":["SMC2 localizes to a discrete centromeric focus during early schizogony without forming condensin I/II complexes, then redistributes diffusely and assembles condensin I and II in mature schizonts and male gametocytes [#0].","SMC2 knockdown is essential for asexual proliferation and male gametogenesis/exflagellation, implicating it in transmission [#0].","Chromosomal loading of SMC2 requires the condensin I kleisin PfCAP-H, which does not affect SMC2 expression level [#1].","SMC2 interactions with EB1 and MAP2 rest on single Co-IP/MS experiments in P. berghei without functional validation [#2, #3]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"32049018","id":"PMC_32049018","title":"Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission.","date":"2020","source":"Cell reports","url":"https://pubmed.ncbi.nlm.nih.gov/32049018","citation_count":30,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"38564676","id":"","title":"PfCAP-H is essential for assembly of condensin I complex and karyokinesis during asexual proliferation of Plasmodium falciparum.","date":"2024","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/38564676","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41387489","id":"","title":"Novel zoonotic cases of Plasmodium simium from São Paulo with a reference genome of the Brazilian strain.","date":"2025","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/41387489","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40127922","id":"","title":"Divergent Plasmodium kinases drive MTOC, kinetochore and axoneme organisation in male gametogenesis.","date":"2025","source":"Life science alliance","url":"https://pubmed.ncbi.nlm.nih.gov/40127922","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"38411954","id":"","title":"Neddylation is essential for malaria transmission in Plasmodium berghei.","date":"2024","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/38411954","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37704606","id":"","title":"Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages.","date":"2023","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/37704606","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36040030","id":"","title":"TurboID Identification of Evolutionarily Divergent Components of the Nuclear Pore Complex in the Malaria Model Plasmodium berghei.","date":"2022","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/36040030","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34835432","id":"","title":"Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini-Chromosome Maintenance Complex Binding Protein.","date":"2021","source":"Microorganisms","url":"https://pubmed.ncbi.nlm.nih.gov/34835432","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34145386","id":"","title":"Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages.","date":"2021","source":"Communications biology","url":"https://pubmed.ncbi.nlm.nih.gov/34145386","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"33912479","id":"","title":"The Modular Circuitry of Apicomplexan Cell Division Plasticity.","date":"2021","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/33912479","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":12225,"output_tokens":1331,"usd":0.02832,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6182,"output_tokens":2448,"usd":0.046055,"stage2_stop_reason":"end_turn"},"total_usd":0.074375,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2020,\n      \"finding\": \"SMC2 (PF3D7_1318400) and SMC4 localize to a distinct centromeric focus during early schizogony (confirmed by NDC80 fluorescence and ChIP-seq), but do not form condensin I or II complexes at this stage. In mature schizonts and during male gametogenesis, SMC2/SMC4 show diffuse chromosomal/nuclear distribution and form both condensin I and condensin II complexes. Knockdown of smc2 reveals an essential role in parasite proliferation (asexual) and transmission (male gametogenesis/exflagellation).\",\n      \"method\": \"Fluorescence live-cell imaging, ChIP-seq (centromere localization), conditional gene knockdown, co-immunoprecipitation (condensin I/II complex formation)\",\n      \"journal\": \"Cell reports\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — multiple orthogonal methods (ChIP-seq, live imaging, Co-IP, conditional knockdown with defined phenotype) in a single focused study on this gene\",\n      \"pmids\": [\"32049018\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"SMC2 (PF3D7_1318400) is a component of the condensin I complex in P. falciparum. Knockout of the condensin I kleisin subunit PfCAP-H does not alter SMC2/SMC4 expression but disrupts their localization at mitotic chromosomes, indicating that PfCAP-H is required for proper loading of the condensin I complex (including SMC2) onto chromosomes during asexual blood-stage division.\",\n      \"method\": \"Inducible DiCre knockout of PfCAP-H, immunofluorescence assay (IFA) for condensin complex localization, immunoblot\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean inducible KO with IFA-based localization readout for SMC2, single lab, two orthogonal methods\",\n      \"pmids\": [\"38564676\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"3D7\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"SMC2 (PF3D7_1318400) and SMC4 co-precipitate with the kinase MAP2-GFP during male gametogenesis in P. berghei, suggesting that SMC2/SMC4 (condensin) interact with MAP2 and may be regulated by this kinase during atypical chromosome condensation associated with the MTOC.\",\n      \"method\": \"Immunoprecipitation/mass spectrometry (GFP pulldown of MAP2) from activated male gametocytes\",\n      \"journal\": \"Life science alliance\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single Co-IP/MS experiment, no reciprocal pulldown or direct functional validation of SMC2 interaction with MAP2\",\n      \"pmids\": [\"40127922\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"SMC2 (PF3D7_1318400) and SMC4 (condensin) are enriched in immunoprecipitates of EB1-GFP from P. berghei gametocytes, placing SMC2 in a protein interaction network at the spindle-kinetochore interface during sexual-stage endomitosis.\",\n      \"method\": \"Immunoprecipitation/mass spectrometry (GFP pulldown of EB1) from activated gametocytes, PCA of peptide spectral counts\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single Co-IP/MS experiment identifying SMC2 as an interactor, no direct functional follow-up on SMC2 itself\",\n      \"pmids\": [\"37704606\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"SMC2 (PF3D7_1318400) is a core condensin subunit in Plasmodium that localizes to centromeres (without forming condensin I or II complexes) during early schizogony, then redistributes diffusely across chromosomes in mature schizonts and male gametocytes where it assembles into both condensin I and condensin II complexes; knockdown of SMC2 is essential for asexual parasite proliferation and male gametogenesis/transmission, and its proper chromosomal loading depends on the condensin I kleisin subunit PfCAP-H.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"SMC2 (PF3D7_1318400) is a core structural maintenance of chromosomes protein that serves as a condensin subunit governing chromosome organization during both asexual proliferation and male gametogenesis in Plasmodium [#0]. During early schizogony SMC2 (together with SMC4) concentrates at a discrete centromeric focus without assembling into condensin I or II complexes, then redistributes diffusely across chromosomes in mature schizonts and activated male gametocytes, where it assembles into both condensin I and condensin II complexes [#0]. Conditional knockdown establishes that SMC2 is essential for asexual parasite proliferation and for male gametogenesis/exflagellation, linking it to parasite transmission [#0]. In P. falciparum, proper chromosomal loading of the condensin I-associated SMC2 depends on the kleisin subunit PfCAP-H, whose knockout disrupts SMC2/SMC4 localization at mitotic chromosomes without altering their expression [#1]. Beyond these findings, the biochemical activity of SMC2 itself has not been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2020,\n      \"claim\": \"Established that SMC2 is a stage-regulated condensin subunit whose localization and complex membership change through the cell cycle, and that it is functionally essential for proliferation and transmission.\",\n      \"evidence\": \"ChIP-seq and live imaging for localization, Co-IP for condensin I/II assembly, and conditional knockdown with defined phenotypes in P. falciparum and P. berghei\",\n      \"pmids\": [\"32049018\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Molecular basis for the centromere-to-diffuse redistribution is unresolved\",\n        \"The trigger for condensin I versus condensin II assembly is not defined\",\n        \"No direct enzymatic or DNA-binding activity of SMC2 demonstrated\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Placed SMC2 within a spindle-kinetochore interaction network during sexual-stage endomitosis, hinting at coordination with the mitotic apparatus.\",\n      \"evidence\": \"EB1-GFP immunoprecipitation/mass spectrometry from activated P. berghei gametocytes\",\n      \"pmids\": [\"37704606\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single Co-IP/MS without reciprocal pulldown or functional follow-up on SMC2\",\n        \"Whether the association is direct or bridged by other condensin subunits is unknown\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Identified the condensin I kleisin PfCAP-H as required for loading SMC2 onto mitotic chromosomes, distinguishing complex assembly from chromosomal recruitment.\",\n      \"evidence\": \"Inducible DiCre knockout of PfCAP-H with IFA-based SMC2/SMC4 localization and immunoblot in P. falciparum 3D7\",\n      \"pmids\": [\"38564676\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Whether SMC2 also requires distinct factors for condensin II loading is untested\",\n        \"Direct SMC2\\u2013PfCAP-H binding interface not defined\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"3D7\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Linked SMC2 to a mitotic kinase, raising the possibility that condensin is regulated during atypical male-gametogenesis chromosome condensation.\",\n      \"evidence\": \"MAP2-GFP immunoprecipitation/mass spectrometry from activated male gametocytes in P. berghei\",\n      \"pmids\": [\"40127922\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single Co-IP/MS without reciprocal pulldown or direct functional validation of an SMC2\\u2013MAP2 interaction\",\n        \"No evidence that MAP2 phosphorylates or directly regulates SMC2\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The intrinsic biochemical activity of SMC2 and the signals driving its stage-specific centromeric-to-diffuse redistribution and condensin I/II assembly remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No structural or ATPase/DNA-engagement data for SMC2 in this corpus\",\n        \"Mechanism switching SMC2 between condensin I and condensin II unresolved\",\n        \"Regulatory kinase inputs to SMC2 not functionally established\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003682\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0000775\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0000793\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0007076\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0007140\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0051301\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"complexes\": [\n      \"condensin I complex\",\n      \"condensin II complex\"\n    ],\n    \"partners\": [\n      \"SMC4\",\n      \"PfCAP-H (condensin I kleisin)\",\n      \"MAP2 kinase (P. berghei, Co-IP/MS)\",\n      \"EB1 (P. berghei, Co-IP/MS)\"\n    ],\n    \"other_free_text\": [\n      \"SMC2 localizes to a discrete centromeric focus during early schizogony without forming condensin I/II complexes, then redistributes diffusely and assembles condensin I and II in mature schizonts and male gametocytes [#0].\",\n      \"SMC2 knockdown is essential for asexual proliferation and male gametogenesis/exflagellation, implicating it in transmission [#0].\",\n      \"Chromosomal loading of SMC2 requires the condensin I kleisin PfCAP-H, which does not affect SMC2 expression level [#1].\",\n      \"SMC2 interactions with EB1 and MAP2 rest on single Co-IP/MS experiments in P. berghei without functional validation [#2, #3].\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1318400","name":null,"product":"structural maintenance of chromosomes protein 2, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1416900","name":null,"product":"structural maintenance of chromosomes protein 2","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1419200","name":null,"product":"structural maintenance of chromosomes protein 2, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"SMC2","evaluation":null}