{"gene":"PF3D7_1315400","run_date":"2026-09-08T14:19:06","timeline":{"discoveries":[{"year":2023,"finding":"PfMD3 (PF3D7_1315400) localizes predominantly to the cytoplasm of immature male P. falciparum gametocytes, and targeted gene disruption impairs gametocyte maturation and male gametocytogenesis.","method":"Immunofluorescence localization; targeted gene knockout with phenotypic analysis","journal":"Molecular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct localization experiment with functional consequence established by KO, single lab with two orthogonal methods","pmids":["38148574"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"finding":"BioID proximity-labeling combined with co-immunoprecipitation revealed that PfMD3 interacts with an RNA-binding protein network including PABP1 and ALBA3, translational initiators/regulators/repressors (eIF4G, PUF1, NOT1, CITH), and gametocytogenesis regulators ZNF4, MD1, and GD1, placing MD3 in a post-transcriptional regulatory complex.","method":"BioID proximity labeling; co-immunoprecipitation","journal":"Molecular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal Co-IP plus BioID with two orthogonal methods, single lab","pmids":["38148574"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"finding":"PfMD3 binds mRNA transcripts associated with male gamete development through a specific RNA sequence motif, and its genetic disruption causes a male fertility defect with decreased infectivity to mosquitoes.","method":"RNA-binding assay (identification of bound transcripts); genetic disruption (KO) with infectivity phenotype","journal":"bioRxiv","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — direct RNA-binding demonstrated with KO phenotype, preprint single lab","pmids":["42244567"],"is_preprint":true,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"finding":"Proteins encoded by PfMD3-bound mRNA transcripts decrease significantly in PfMD3 knockout parasites, indicating that PfMD3 promotes translation of male gamete development transcripts rather than merely stabilizing them.","method":"Quantitative proteomics comparing WT and PfMD3-KO gametocytes; RNA-binding assay","journal":"bioRxiv","confidence":"Medium","confidence_rationale":"Tier 1–2 / Moderate — proteomic quantification combined with RNA-binding data in KO, preprint single lab with two orthogonal methods","pmids":["42244567"],"is_preprint":true,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"finding":"PfMD3 interacts with proteins involved in mRNA processing and nuclear export that influence translational regulation, consistent with its role in post-transcriptional control.","method":"Co-immunoprecipitation / protein interaction assays","journal":"bioRxiv","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single Co-IP interaction reported in a preprint, no independent replication","pmids":["42244567"],"is_preprint":true,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_1315400 (PfMD3) is a cytoplasmic CCCH-type zinc finger RNA-binding protein expressed in immature male gametocytes that binds specific mRNA transcripts encoding male gamete development proteins, promotes their translation, and functions within a post-transcriptional regulatory complex (including PABP1, ALBA3, eIF4G, PUF1, NOT1, CITH, ZNF4, MD1, GD1) to drive male gametocytogenesis and mosquito infectivity."},"narrative":{"mechanistic_narrative":"PF3D7_1315400 (PfMD3) is a cytoplasmic CCCH-type zinc finger RNA-binding protein expressed in immature male Plasmodium falciparum gametocytes that acts in post-transcriptional control to drive male gametocytogenesis and mosquito infectivity [PMID:38148574, PMID:42244567]. It binds a defined set of mRNA transcripts encoding male gamete development proteins through a specific RNA sequence motif and promotes their translation: proteins encoded by PfMD3-bound transcripts fall sharply in knockout parasites, establishing a translation-promoting rather than purely stabilizing role [PMID:42244567]. PfMD3 operates within an RNA-binding protein network that includes PABP1 and ALBA3, translational initiators/regulators/repressors (eIF4G, PUF1, NOT1, CITH), and the gametocytogenesis regulators ZNF4, MD1, and GD1 [PMID:38148574]. Consistent with these interactions, targeted disruption of PfMD3 impairs gametocyte maturation and male gametocytogenesis and produces a male fertility defect with reduced infectivity to mosquitoes [PMID:38148574, PMID:42244567]. Beyond this post-transcriptional regulatory role in male gamete development, no further mechanistic detail has been characterized in the available corpus.","teleology":[{"year":2023,"claim":"Establishing where PfMD3 acts and whether it matters resolved its cellular compartment and stage-specific requirement, linking it to male gametocyte development.","evidence":"Immunofluorescence localization and targeted gene knockout with phenotypic analysis in P. falciparum gametocytes","pmids":["38148574"],"confidence":"Medium","gaps":["Does not identify the RNA or protein targets through which PfMD3 acts","Molecular activity of the protein not defined by localization/KO alone"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"claim":"Defining PfMD3's protein neighborhood placed it inside a post-transcriptional regulatory complex rather than acting in isolation.","evidence":"BioID proximity labeling plus reciprocal co-immunoprecipitation identifying PABP1, ALBA3, eIF4G, PUF1, NOT1, CITH, ZNF4, MD1, GD1 in P. falciparum","pmids":["38148574"],"confidence":"Medium","gaps":["Direct versus proximity-mediated nature of each interaction not resolved","Does not establish whether PfMD3 represses or promotes translation of complex targets","Stoichiometry and architecture of the complex unknown"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"claim":"Identifying that PfMD3 binds specific male-gamete-development mRNAs via a sequence motif established it as a sequence-specific RNA-binding regulator underlying the fertility phenotype.","evidence":"RNA-binding assay identifying bound transcripts and motif, with KO infectivity phenotype in P. falciparum (preprint)","pmids":["42244567"],"confidence":"Medium","gaps":["Preprint, single lab, not independently confirmed","Structural basis of motif recognition not determined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"claim":"Quantifying target protein levels in knockout parasites distinguished translational promotion from mRNA stabilization, defining the directionality of PfMD3's regulatory effect.","evidence":"Quantitative proteomics comparing WT and PfMD3-KO gametocytes combined with RNA-binding data in P. falciparum (preprint)","pmids":["42244567"],"confidence":"Medium","gaps":["Preprint, single lab, not independently confirmed","Whether decreased protein reflects direct translational activation versus indirect network effects not separated","mRNA-level measurements needed to fully exclude stabilization contribution"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"claim":"Detecting PfMD3 interactions with mRNA processing and nuclear export factors extended its regulatory reach toward earlier steps of the RNA lifecycle.","evidence":"Co-immunoprecipitation / protein interaction assays in P. falciparum (preprint)","pmids":["42244567"],"confidence":"Low","gaps":["Single Co-IP in a preprint with no independent replication or reciprocal validation","Functional consequence of the processing/export interactions not tested","Identity of specific partners not resolved into a defined mechanism"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"How PfMD3's zinc finger recognizes its target motif and how the surrounding complex switches its regulatory output remain unresolved.","evidence":"","pmids":[],"confidence":"Low","gaps":["No structural model of the CCCH zinc finger bound to its RNA motif","No mechanism for how PfMD3 recruits or is recruited within the complex","Whether findings hold in other Plasmodium species is untested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Post-transcriptional regulatory RNA-binding protein complex containing PABP1, ALBA3, eIF4G, PUF1, NOT1, CITH, ZNF4, MD1, GD1 [#1]"],"partners":["PABP1 [#1]","ALBA3 [#1]","EIF4G [#1]","PUF1 [#1]","NOT1 [#1]","CITH [#1]","ZNF4 [#1]","MD1 [#1]","GD1 [#1]"],"other_free_text":["Binds male gamete development mRNA transcripts through a specific RNA sequence motif [#2]","Promotes translation of bound transcripts; target proteins decrease in KO gametocytes [#3]","Disruption impairs male gametocytogenesis and reduces mosquito infectivity [#0, #2]","Interacts with mRNA processing and nuclear export factors (Low confidence, preprint) [#4]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"15256513","id":"PMC_15256513","title":"Comparative genomics of transcriptional control in the human malaria parasite Plasmodium falciparum.","date":"2004","source":"Genome research","url":"https://pubmed.ncbi.nlm.nih.gov/15256513","citation_count":191,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CCCH"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34418264","id":"PMC_34418264","title":"Zinc finger proteins of Plasmodium falciparum.","date":"2021","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/34418264","citation_count":33,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CCCH"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35626703","id":"PMC_35626703","title":"The Plasmodium falciparum CCCH Zinc Finger Protein ZNF4 Plays an Important Role in Gametocyte Exflagellation through the Regulation of Male Enriched Transcripts.","date":"2022","source":"Cells","url":"https://pubmed.ncbi.nlm.nih.gov/35626703","citation_count":20,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CCCH","PfCCCH"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"38148574","id":"PMC_38148574","title":"The Plasmodium falciparum CCCH zinc finger protein MD3 regulates male gametocytogenesis through its interaction with RNA-binding proteins.","date":"2023","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/38148574","citation_count":8,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CCCH"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"18769675","id":"PMC_18769675","title":"Computational analysis of constraints on noncoding regions, coding regions and gene expression in relation to Plasmodium phenotypic diversity.","date":"2008","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/18769675","citation_count":6,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CCCH"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36400305","id":"PMC_36400305","title":"Characterisation of PfCZIF1 and PfCZIF2 in Plasmodium falciparum asexual stages.","date":"2022","source":"International journal for parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/36400305","citation_count":5,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CCCH"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42244567","id":"PMC_42244567","title":"The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission.","date":"2026","source":"bioRxiv : the preprint server for biology","url":"https://pubmed.ncbi.nlm.nih.gov/42244567","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CCCH"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":6010,"output_tokens":1664,"usd":0.021495,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6054,"output_tokens":2675,"usd":0.048572,"stage2_stop_reason":"end_turn"},"total_usd":0.070067,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"I need to carefully identify which papers are about PF3D7_1315400 specifically.\n\nLooking at the papers:\n- Papers [3] and [6] are about other CCCH zinc finger proteins (ZNF4 = different gene, PfCZIF1/PfCZIF2 = different genes with explicit IDs Pf3D7_1468400 and Pf3D7_0818100)\n- Papers [4] and [7] are about MD3 (Male Development 3, PfMD3) — I need to check if this is PF3D7_1315400\n- Papers [1], [2], [5] are general/comparative genomics papers about CCCH zinc fingers in Plasmodium\n\nPaper [4] mentions \"MD3 was originally identified as an epigenetically regulated protein of immature gametocytes and recently shown to be involved in male development in a barcode-based screen in P. berghei.\" Paper [7] is explicitly about \"Plasmodium falciparum Male Development 3 (PfMD3).\" The gene PF3D7_1315400 is annotated as a CCCH-type zinc finger protein. Based on the corpus context, MD3 appears to be PF3D7_1315400 (as papers [4] and [7] both study a CCCH-type zinc finger called MD3, and paper [4] mentions its interaction with ZNF4, which is a separate gene). I'll treat MD3 as PF3D7_1315400 based on the corpus context, noting that papers [4] and [7] both study it specifically. Papers [1], [2], [5] are pan-gene catalogs or reviews. Paper [3] is about ZNF4, not MD3/PF3D7_1315400, though it mentions ZNF4 interacts with MD3 (from paper [4]).\n\n```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2023,\n      \"finding\": \"PfMD3 (PF3D7_1315400) localizes predominantly to the cytoplasm of immature male P. falciparum gametocytes, and targeted gene disruption impairs gametocyte maturation and male gametocytogenesis.\",\n      \"method\": \"Immunofluorescence localization; targeted gene knockout with phenotypic analysis\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization experiment with functional consequence established by KO, single lab with two orthogonal methods\",\n      \"pmids\": [\"38148574\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"BioID proximity-labeling combined with co-immunoprecipitation revealed that PfMD3 interacts with an RNA-binding protein network including PABP1 and ALBA3, translational initiators/regulators/repressors (eIF4G, PUF1, NOT1, CITH), and gametocytogenesis regulators ZNF4, MD1, and GD1, placing MD3 in a post-transcriptional regulatory complex.\",\n      \"method\": \"BioID proximity labeling; co-immunoprecipitation\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal Co-IP plus BioID with two orthogonal methods, single lab\",\n      \"pmids\": [\"38148574\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"PfMD3 binds mRNA transcripts associated with male gamete development through a specific RNA sequence motif, and its genetic disruption causes a male fertility defect with decreased infectivity to mosquitoes.\",\n      \"method\": \"RNA-binding assay (identification of bound transcripts); genetic disruption (KO) with infectivity phenotype\",\n      \"journal\": \"bioRxiv\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — direct RNA-binding demonstrated with KO phenotype, preprint single lab\",\n      \"pmids\": [\"42244567\"],\n      \"is_preprint\": true,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"Proteins encoded by PfMD3-bound mRNA transcripts decrease significantly in PfMD3 knockout parasites, indicating that PfMD3 promotes translation of male gamete development transcripts rather than merely stabilizing them.\",\n      \"method\": \"Quantitative proteomics comparing WT and PfMD3-KO gametocytes; RNA-binding assay\",\n      \"journal\": \"bioRxiv\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1–2 / Moderate — proteomic quantification combined with RNA-binding data in KO, preprint single lab with two orthogonal methods\",\n      \"pmids\": [\"42244567\"],\n      \"is_preprint\": true,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"PfMD3 interacts with proteins involved in mRNA processing and nuclear export that influence translational regulation, consistent with its role in post-transcriptional control.\",\n      \"method\": \"Co-immunoprecipitation / protein interaction assays\",\n      \"journal\": \"bioRxiv\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single Co-IP interaction reported in a preprint, no independent replication\",\n      \"pmids\": [\"42244567\"],\n      \"is_preprint\": true,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_1315400 (PfMD3) is a cytoplasmic CCCH-type zinc finger RNA-binding protein expressed in immature male gametocytes that binds specific mRNA transcripts encoding male gamete development proteins, promotes their translation, and functions within a post-transcriptional regulatory complex (including PABP1, ALBA3, eIF4G, PUF1, NOT1, CITH, ZNF4, MD1, GD1) to drive male gametocytogenesis and mosquito infectivity.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1315400 (PfMD3) is a cytoplasmic CCCH-type zinc finger RNA-binding protein expressed in immature male Plasmodium falciparum gametocytes that acts in post-transcriptional control to drive male gametocytogenesis and mosquito infectivity [#0, #2]. It binds a defined set of mRNA transcripts encoding male gamete development proteins through a specific RNA sequence motif and promotes their translation: proteins encoded by PfMD3-bound transcripts fall sharply in knockout parasites, establishing a translation-promoting rather than purely stabilizing role [#2, #3]. PfMD3 operates within an RNA-binding protein network that includes PABP1 and ALBA3, translational initiators/regulators/repressors (eIF4G, PUF1, NOT1, CITH), and the gametocytogenesis regulators ZNF4, MD1, and GD1 [#1]. Consistent with these interactions, targeted disruption of PfMD3 impairs gametocyte maturation and male gametocytogenesis and produces a male fertility defect with reduced infectivity to mosquitoes [#0, #2]. Beyond this post-transcriptional regulatory role in male gamete development, no further mechanistic detail has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2023,\n      \"claim\": \"Establishing where PfMD3 acts and whether it matters resolved its cellular compartment and stage-specific requirement, linking it to male gametocyte development.\",\n      \"evidence\": \"Immunofluorescence localization and targeted gene knockout with phenotypic analysis in P. falciparum gametocytes\",\n      \"pmids\": [\"38148574\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Does not identify the RNA or protein targets through which PfMD3 acts\",\n        \"Molecular activity of the protein not defined by localization/KO alone\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Defining PfMD3's protein neighborhood placed it inside a post-transcriptional regulatory complex rather than acting in isolation.\",\n      \"evidence\": \"BioID proximity labeling plus reciprocal co-immunoprecipitation identifying PABP1, ALBA3, eIF4G, PUF1, NOT1, CITH, ZNF4, MD1, GD1 in P. falciparum\",\n      \"pmids\": [\"38148574\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct versus proximity-mediated nature of each interaction not resolved\",\n        \"Does not establish whether PfMD3 represses or promotes translation of complex targets\",\n        \"Stoichiometry and architecture of the complex unknown\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Identifying that PfMD3 binds specific male-gamete-development mRNAs via a sequence motif established it as a sequence-specific RNA-binding regulator underlying the fertility phenotype.\",\n      \"evidence\": \"RNA-binding assay identifying bound transcripts and motif, with KO infectivity phenotype in P. falciparum (preprint)\",\n      \"pmids\": [\"42244567\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Preprint, single lab, not independently confirmed\",\n        \"Structural basis of motif recognition not determined\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Quantifying target protein levels in knockout parasites distinguished translational promotion from mRNA stabilization, defining the directionality of PfMD3's regulatory effect.\",\n      \"evidence\": \"Quantitative proteomics comparing WT and PfMD3-KO gametocytes combined with RNA-binding data in P. falciparum (preprint)\",\n      \"pmids\": [\"42244567\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Preprint, single lab, not independently confirmed\",\n        \"Whether decreased protein reflects direct translational activation versus indirect network effects not separated\",\n        \"mRNA-level measurements needed to fully exclude stabilization contribution\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Detecting PfMD3 interactions with mRNA processing and nuclear export factors extended its regulatory reach toward earlier steps of the RNA lifecycle.\",\n      \"evidence\": \"Co-immunoprecipitation / protein interaction assays in P. falciparum (preprint)\",\n      \"pmids\": [\"42244567\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single Co-IP in a preprint with no independent replication or reciprocal validation\",\n        \"Functional consequence of the processing/export interactions not tested\",\n        \"Identity of specific partners not resolved into a defined mechanism\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How PfMD3's zinc finger recognizes its target motif and how the surrounding complex switches its regulatory output remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No structural model of the CCCH zinc finger bound to its RNA motif\",\n        \"No mechanism for how PfMD3 recruits or is recruited within the complex\",\n        \"Whether findings hold in other Plasmodium species is untested\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003729\", \"supporting_discovery_ids\": [2, 3]},\n      {\"term_id\": \"GO:0000900\", \"supporting_discovery_ids\": [3]},\n      {\"term_id\": \"GO:0003676\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005737\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006417\", \"supporting_discovery_ids\": [1, 3]},\n      {\"term_id\": \"GO:0007140\", \"supporting_discovery_ids\": [0, 2]}\n    ],\n    \"complexes\": [\n      \"Post-transcriptional regulatory RNA-binding protein complex containing PABP1, ALBA3, eIF4G, PUF1, NOT1, CITH, ZNF4, MD1, GD1 [#1]\"\n    ],\n    \"partners\": [\n      \"PABP1 [#1]\",\n      \"ALBA3 [#1]\",\n      \"eIF4G [#1]\",\n      \"PUF1 [#1]\",\n      \"NOT1 [#1]\",\n      \"CITH [#1]\",\n      \"ZNF4 [#1]\",\n      \"MD1 [#1]\",\n      \"GD1 [#1]\"\n    ],\n    \"other_free_text\": [\n      \"Binds male gamete development mRNA transcripts through a specific RNA sequence motif [#2]\",\n      \"Promotes translation of bound transcripts; target proteins decrease in KO gametocytes [#3]\",\n      \"Disruption impairs male gametocytogenesis and reduces mosquito infectivity [#0, #2]\",\n      \"Interacts with mRNA processing and nuclear export factors (Low confidence, preprint) [#4]\"\n    ]\n  }\n}","audit_flag":{"gene":"PF3D7_1315400","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 2 of 2 cited papers name other catalogue genes (PF3D7_0315600, PF3D7_0927200, PF3D7_1006200). The summary may describe one of those genes."},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1315400","name":null,"product":"zinc finger (CCCH type) protein, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1413900","name":null,"product":"zinc finger (CCCH type) protein, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1416100","name":null,"product":"zinc finger (CCCH type) protein, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"","evaluation":null}