{"gene":"PF3D7_1144700","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2020,"finding":"TIC20 (PF3D7_1144700) is identified as a putative pore-forming component of the Translocon of the Inner Chloroplast membrane (TIC) complex at the innermost membrane of the apicoplast, proposed to mediate translocation of nuclear-encoded proteins into the apicoplast stroma, by analogy with the chloroplast TIC machinery and supported by identification of homologs in Plasmodium and Toxoplasma genomes.","method":"Bioinformatic identification and review of genetic/functional data from T. gondii orthologs; no direct in vitro or in vivo experiment on PF3D7_1144700 itself reported in this abstract","journal":"Cellular microbiology","confidence":"Low","confidence_rationale":"Tier 4 / Weak — computational/comparative inference; no direct experiment on the P. falciparum protein reported in the abstract","pmids":["32388921"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2011,"finding":"TIC20 is listed among a small number of putative apicoplast import components (alongside TIC22 and TOC75) whose critical role in import has been validated through mutant analysis (referencing T. gondii genetic studies); the abstract groups TIC20 with components whose essentiality for apicoplast protein import is supported by genetic evidence in apicomplexans.","method":"Review citing mutant analysis in T. gondii and bioinformatic identification of the homolog in Plasmodium; no direct experiment on PF3D7_1144700 in this paper","journal":"PLoS pathogens","confidence":"Low","confidence_rationale":"Tier 4 / Weak — identification and functional inference based on T. gondii ortholog data cited in a screen paper; no direct experiment on the P. falciparum protein","pmids":["22144892"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2013,"finding":"The ablation of the apicoplast TIC component Tic20 in T. gondii blocks apicoplast protein import and produces a non-viable phenotype closely resembling ablation of the ERAD component Der1Ap, placing TIC20 in the same essential import pathway at the inner apicoplast membrane.","method":"Conditional gene ablation in T. gondii (cited as van Dooren et al. 2008 within the abstract context); epistasis with Der1Ap mutant phenotype","journal":"PLoS pathogens","confidence":"Low","confidence_rationale":"Tier 2 / Weak — genetic ablation with defined phenotype, but performed in T. gondii (not P. falciparum), and the P. falciparum protein PF3D7_1144700 is only inferred by homology; single lab, referenced indirectly","pmids":["23785288"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2017,"finding":"A 3D structural model of P. falciparum TIC20 (PF3D7_1144700) was generated using the I-TASSER server, and in silico docking of antimalarial compounds (quinine, chloroquine, artesunate) into the predicted active site was used to propose druggability; gene ontology and nuclear localization signal motif analysis supported its annotation as an apicoplast import protein.","method":"In silico homology modeling (I-TASSER), in silico docking, gene ontology analysis, NLS motif analysis","journal":"Infectious disorders drug targets","confidence":"Low","confidence_rationale":"Tier 4 / Weak — purely computational; no wet-lab experiment performed on PF3D7_1144700","pmids":["28595540"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_1144700 (TIC20) is a putative pore-forming component of the Translocon of the Inner Chloroplast membrane (TIC) at the innermost apicoplast membrane of P. falciparum, inferred by homology to T. gondii TIC20 whose genetic ablation blocks apicoplast protein import and causes parasite death; no direct in vivo or in vitro experiment has yet been reported for the P. falciparum protein itself."},"narrative":{"mechanistic_narrative":"PF3D7_1144700 (TIC20) is annotated as a putative pore-forming component of the Translocon of the Inner Chloroplast membrane (TIC) complex at the innermost membrane of the P. falciparum apicoplast, where it is proposed to mediate translocation of nuclear-encoded proteins into the apicoplast stroma [PMID:32388921]. This assignment rests entirely on homology to characterized apicomplexan orthologs rather than on direct experiments with the P. falciparum protein: TIC20 is grouped with TIC22 and TOC75 as import components whose essentiality is inferred from T. gondii genetic data [PMID:22144892], and in T. gondii the ortholog's conditional ablation blocks apicoplast protein import and yields a non-viable phenotype resembling loss of the ERAD-derived component Der1Ap, placing it in the inner-membrane import pathway [PMID:23785288]. A computational structural model of PF3D7_1144700 has been built and used for in silico docking of antimalarials, but no wet-lab characterization accompanied it [PMID:28595540]. Beyond these homology-based and computational inferences, no direct in vitro or in vivo experiment on PF3D7_1144700 itself has been reported in the available corpus, and its pore-forming activity, interaction partners, and import function in P. falciparum remain uncharacterized.","teleology":[{"year":2011,"claim":"To account for how nuclear-encoded proteins cross the innermost apicoplast membrane, TIC20 was placed among the candidate import components, establishing it as a member of the putative apicoplast TIC machinery.","evidence":"Review citing T. gondii mutant analysis and bioinformatic identification of the P. falciparum homolog; no direct experiment on PF3D7_1144700","pmids":["22144892"],"confidence":"Low","gaps":["No direct experiment on the P. falciparum protein; membership is inferred from T. gondii ortholog data","Import function of PF3D7_1144700 not tested","No evidence of physical association with TIC22 or TOC75 in P. falciparum"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2013,"claim":"Whether TIC20 is functionally essential for apicoplast protein import was addressed in the T. gondii ortholog, showing that its ablation blocks import with a phenotype resembling loss of the ERAD component Der1Ap, positioning it in a defined inner-membrane import pathway.","evidence":"Conditional gene ablation in T. gondii with epistasis to the Der1Ap mutant phenotype","pmids":["23785288"],"confidence":"Low","gaps":["Not demonstrated for P. falciparum PF3D7_1144700, which is inferred by homology only","Mechanism of translocation (pore formation) not directly shown","Molecular connection between TIC20 and the ERAD-derived machinery not resolved at the protein level"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2017,"claim":"To probe the druggability of PF3D7_1144700, a computational structural model and in silico docking of antimalarials were used, reinforcing its annotation as an apicoplast import protein but without wet-lab validation.","evidence":"In silico homology modeling (I-TASSER), docking, gene ontology and NLS motif analysis","pmids":["28595540"],"confidence":"Low","gaps":["Purely computational; no experimental structure or binding assay","Predicted active site and druggability not experimentally verified","No functional validation of the modeled protein"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2020,"claim":"The prevailing model consolidates PF3D7_1144700 as a putative pore-forming TIC component mediating protein translocation across the innermost apicoplast membrane, framing the outstanding need for direct experimental testing.","evidence":"Bioinformatic identification and review of T. gondii ortholog genetic/functional data; no direct experiment on PF3D7_1144700","pmids":["32388921"],"confidence":"Low","gaps":["Pore-forming activity of PF3D7_1144700 never demonstrated","No P. falciparum genetic ablation or complementation reported","Interaction partners and complex composition in P. falciparum undefined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"No direct in vitro or in vivo experiment on P. falciparum PF3D7_1144700 has established its pore-forming activity, essentiality, or import function; all current claims are homology-based or computational.","evidence":"","pmids":[],"confidence":"Low","gaps":["No experimental localization to the inner apicoplast membrane in P. falciparum","No demonstration of protein translocation activity","No mapped physical interactions with other TIC components in P. falciparum"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Translocon of the Inner Chloroplast membrane (TIC) complex (putative, by homology) [#0, #1]"],"partners":["TIC22, TOC75 (CO-LISTED PUTATIVE APICOPLAST IMPORT COMPONENTS; NO DIRECT INTERACTION SHOWN FOR PF3D7_1144700) [#1]","DER1AP (SAME IMPORT PATHWAY BY T. GONDII ORTHOLOG EPISTASIS; NO DIRECT INTERACTION SHOWN FOR PF3D7_1144700) [#2]"],"other_free_text":["All functional assignments for PF3D7_1144700 derive from homology to T. gondii TIC20 and computational analysis; no direct wet-lab experiment on the P. falciparum protein is reported in the corpus [#0, #1, #2, #3].","A computational 3D structural model (I-TASSER) and in silico antimalarial docking have been performed to propose druggability [#3]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"28595540","id":"PMC_28595540","title":"Apicoplast Import Protein Tic20 A Promising Therapeutic Molecular Target for Plasmodium falciparum: An In Silico Approach for Therapeutic Intervention.","date":"2017","source":"Infectious disorders drug targets","url":"https://pubmed.ncbi.nlm.nih.gov/28595540","citation_count":2,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20","apicoplast import protein Tic20","Tic20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39575308","id":"","title":"Iron transport pathways in the human malaria parasite Plasmodium falciparum revealed by RNA-sequencing.","date":"2024","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/39575308","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"32388921","id":"","title":"Directing traffic: Chaperone-mediated protein transport in malaria parasites.","date":"2020","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/32388921","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"22072821","id":"","title":"Expressed sequence tag analysis of the erythrocytic stage of Plasmodium berghei.","date":"2011","source":"The Korean journal of parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/22072821","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"23785288","id":"","title":"An apicoplast localized ubiquitylation system is required for the import of nuclear-encoded plastid proteins.","date":"2013","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/23785288","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"24572015","id":"","title":"Chromera velia, endosymbioses and the rhodoplex hypothesis--plastid evolution in cryptophytes, alveolates, stramenopiles, and haptophytes (CASH lineages).","date":"2014","source":"Genome biology and evolution","url":"https://pubmed.ncbi.nlm.nih.gov/24572015","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"20124342","id":"","title":"The evolution, metabolism and functions of the apicoplast.","date":"2010","source":"Philosophical transactions of the Royal Society of London. Series B, Biological sciences","url":"https://pubmed.ncbi.nlm.nih.gov/20124342","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"19835607","id":"","title":"Plastid genomes of two brown algae, Ectocarpus siliculosus and Fucus vesiculosus: further insights on the evolution of red-algal derived plastids.","date":"2009","source":"BMC evolutionary biology","url":"https://pubmed.ncbi.nlm.nih.gov/19835607","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"21333023","id":"","title":"Endosymbiont or host: who drove mitochondrial and plastid evolution?","date":"2011","source":"Biology direct","url":"https://pubmed.ncbi.nlm.nih.gov/21333023","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"22144892","id":"","title":"A systematic screen to discover and analyze apicoplast proteins identifies a conserved and essential protein import factor.","date":"2011","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/22144892","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["TIC20"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":11432,"output_tokens":1251,"usd":0.02653,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6085,"output_tokens":2407,"usd":0.0453,"stage2_stop_reason":"end_turn"},"total_usd":0.07183,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2020,\n      \"finding\": \"TIC20 (PF3D7_1144700) is identified as a putative pore-forming component of the Translocon of the Inner Chloroplast membrane (TIC) complex at the innermost membrane of the apicoplast, proposed to mediate translocation of nuclear-encoded proteins into the apicoplast stroma, by analogy with the chloroplast TIC machinery and supported by identification of homologs in Plasmodium and Toxoplasma genomes.\",\n      \"method\": \"Bioinformatic identification and review of genetic/functional data from T. gondii orthologs; no direct in vitro or in vivo experiment on PF3D7_1144700 itself reported in this abstract\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — computational/comparative inference; no direct experiment on the P. falciparum protein reported in the abstract\",\n      \"pmids\": [\"32388921\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2011,\n      \"finding\": \"TIC20 is listed among a small number of putative apicoplast import components (alongside TIC22 and TOC75) whose critical role in import has been validated through mutant analysis (referencing T. gondii genetic studies); the abstract groups TIC20 with components whose essentiality for apicoplast protein import is supported by genetic evidence in apicomplexans.\",\n      \"method\": \"Review citing mutant analysis in T. gondii and bioinformatic identification of the homolog in Plasmodium; no direct experiment on PF3D7_1144700 in this paper\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — identification and functional inference based on T. gondii ortholog data cited in a screen paper; no direct experiment on the P. falciparum protein\",\n      \"pmids\": [\"22144892\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2013,\n      \"finding\": \"The ablation of the apicoplast TIC component Tic20 in T. gondii blocks apicoplast protein import and produces a non-viable phenotype closely resembling ablation of the ERAD component Der1Ap, placing TIC20 in the same essential import pathway at the inner apicoplast membrane.\",\n      \"method\": \"Conditional gene ablation in T. gondii (cited as van Dooren et al. 2008 within the abstract context); epistasis with Der1Ap mutant phenotype\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 2 / Weak — genetic ablation with defined phenotype, but performed in T. gondii (not P. falciparum), and the P. falciparum protein PF3D7_1144700 is only inferred by homology; single lab, referenced indirectly\",\n      \"pmids\": [\"23785288\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"finding\": \"A 3D structural model of P. falciparum TIC20 (PF3D7_1144700) was generated using the I-TASSER server, and in silico docking of antimalarial compounds (quinine, chloroquine, artesunate) into the predicted active site was used to propose druggability; gene ontology and nuclear localization signal motif analysis supported its annotation as an apicoplast import protein.\",\n      \"method\": \"In silico homology modeling (I-TASSER), in silico docking, gene ontology analysis, NLS motif analysis\",\n      \"journal\": \"Infectious disorders drug targets\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — purely computational; no wet-lab experiment performed on PF3D7_1144700\",\n      \"pmids\": [\"28595540\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_1144700 (TIC20) is a putative pore-forming component of the Translocon of the Inner Chloroplast membrane (TIC) at the innermost apicoplast membrane of P. falciparum, inferred by homology to T. gondii TIC20 whose genetic ablation blocks apicoplast protein import and causes parasite death; no direct in vivo or in vitro experiment has yet been reported for the P. falciparum protein itself.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1144700 (TIC20) is annotated as a putative pore-forming component of the Translocon of the Inner Chloroplast membrane (TIC) complex at the innermost membrane of the P. falciparum apicoplast, where it is proposed to mediate translocation of nuclear-encoded proteins into the apicoplast stroma [#0]. This assignment rests entirely on homology to characterized apicomplexan orthologs rather than on direct experiments with the P. falciparum protein: TIC20 is grouped with TIC22 and TOC75 as import components whose essentiality is inferred from T. gondii genetic data [#1], and in T. gondii the ortholog's conditional ablation blocks apicoplast protein import and yields a non-viable phenotype resembling loss of the ERAD-derived component Der1Ap, placing it in the inner-membrane import pathway [#2]. A computational structural model of PF3D7_1144700 has been built and used for in silico docking of antimalarials, but no wet-lab characterization accompanied it [#3]. Beyond these homology-based and computational inferences, no direct in vitro or in vivo experiment on PF3D7_1144700 itself has been reported in the available corpus, and its pore-forming activity, interaction partners, and import function in P. falciparum remain uncharacterized.\",\n  \"teleology\": [\n    {\n      \"year\": 2011,\n      \"claim\": \"To account for how nuclear-encoded proteins cross the innermost apicoplast membrane, TIC20 was placed among the candidate import components, establishing it as a member of the putative apicoplast TIC machinery.\",\n      \"evidence\": \"Review citing T. gondii mutant analysis and bioinformatic identification of the P. falciparum homolog; no direct experiment on PF3D7_1144700\",\n      \"pmids\": [\"22144892\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No direct experiment on the P. falciparum protein; membership is inferred from T. gondii ortholog data\",\n        \"Import function of PF3D7_1144700 not tested\",\n        \"No evidence of physical association with TIC22 or TOC75 in P. falciparum\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2013,\n      \"claim\": \"Whether TIC20 is functionally essential for apicoplast protein import was addressed in the T. gondii ortholog, showing that its ablation blocks import with a phenotype resembling loss of the ERAD component Der1Ap, positioning it in a defined inner-membrane import pathway.\",\n      \"evidence\": \"Conditional gene ablation in T. gondii with epistasis to the Der1Ap mutant phenotype\",\n      \"pmids\": [\"23785288\"],\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Performed in T. gondii, not P. falciparum\",\n      \"gaps\": [\n        \"Not demonstrated for P. falciparum PF3D7_1144700, which is inferred by homology only\",\n        \"Mechanism of translocation (pore formation) not directly shown\",\n        \"Molecular connection between TIC20 and the ERAD-derived machinery not resolved at the protein level\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"claim\": \"To probe the druggability of PF3D7_1144700, a computational structural model and in silico docking of antimalarials were used, reinforcing its annotation as an apicoplast import protein but without wet-lab validation.\",\n      \"evidence\": \"In silico homology modeling (I-TASSER), docking, gene ontology and NLS motif analysis\",\n      \"pmids\": [\"28595540\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Purely computational; no experimental structure or binding assay\",\n        \"Predicted active site and druggability not experimentally verified\",\n        \"No functional validation of the modeled protein\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2020,\n      \"claim\": \"The prevailing model consolidates PF3D7_1144700 as a putative pore-forming TIC component mediating protein translocation across the innermost apicoplast membrane, framing the outstanding need for direct experimental testing.\",\n      \"evidence\": \"Bioinformatic identification and review of T. gondii ortholog genetic/functional data; no direct experiment on PF3D7_1144700\",\n      \"pmids\": [\"32388921\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Pore-forming activity of PF3D7_1144700 never demonstrated\",\n        \"No P. falciparum genetic ablation or complementation reported\",\n        \"Interaction partners and complex composition in P. falciparum undefined\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"No direct in vitro or in vivo experiment on P. falciparum PF3D7_1144700 has established its pore-forming activity, essentiality, or import function; all current claims are homology-based or computational.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No experimental localization to the inner apicoplast membrane in P. falciparum\",\n        \"No demonstration of protein translocation activity\",\n        \"No mapped physical interactions with other TIC components in P. falciparum\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0015450\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0009528\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0045037\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"complexes\": [\n      \"Translocon of the Inner Chloroplast membrane (TIC) complex (putative, by homology) [#0, #1]\"\n    ],\n    \"partners\": [\n      \"TIC22, TOC75 (co-listed putative apicoplast import components; no direct interaction shown for PF3D7_1144700) [#1]\",\n      \"Der1Ap (same import pathway by T. gondii ortholog epistasis; no direct interaction shown for PF3D7_1144700) [#2]\"\n    ],\n    \"other_free_text\": [\n      \"All functional assignments for PF3D7_1144700 derive from homology to T. gondii TIC20 and computational analysis; no direct wet-lab experiment on the P. falciparum protein is reported in the corpus [#0, #1, #2, #3].\",\n      \"A computational 3D structural model (I-TASSER) and in silico antimalarial docking have been performed to propose druggability [#3].\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1144700","name":null,"product":"apicoplast import protein Tic20, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0904300","name":null,"product":"apicoplast import protein Tic20, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0942600","name":null,"product":"apicoplast import protein Tic20, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"TIC20","evaluation":null}