{"gene":"PF3D7_1143100","run_date":"2026-09-08T14:19:05","timeline":{"discoveries":[{"year":2009,"finding":"AP2-O (PF3D7_1143100) was identified as a transcription factor that activates gene expression in ookinetes, the mosquito-invasive form of Plasmodium. Its mRNA is pre-synthesized by intraerythrocytic female gametocytes and translationally repressed, then translated during ookinete development. AP2-O activates a set of genes required for mosquito midgut invasion by binding to specific six-base sequences on proximal promoters.","method":"Genetic disruption of AP2-O, transcriptome analysis, promoter binding assays, and stage-specific expression profiling in Plasmodium berghei","journal":"Molecular microbiology","confidence":"High","confidence_rationale":"Tier 2 / Strong — loss-of-function with defined cellular and transcriptional phenotype, DNA-binding specificity demonstrated, translational regulation characterized; foundational study replicated by subsequent work","pmids":["19220746"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2009,"finding":"In transgenic P. berghei parasites expressing endogenous AP2-O but with its AP2 DNA-binding domain swapped with that of AP2-Sp, several AP2-Sp target genes were induced in the ookinete stage, demonstrating that the DNA-binding domain of AP2-O determines target gene specificity and that AP2-O's transcriptional activation function is domain-swappable.","method":"Domain-swap transgenic experiment in P. berghei: AP2-O coding region with AP2-Sp DNA-binding domain inserted; target gene expression analyzed","journal":"Molecular microbiology","confidence":"High","confidence_rationale":"Tier 1 / Moderate — direct domain-swap mutagenesis with functional readout in vivo, single lab but mechanistically rigorous","pmids":["20025671"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2017,"finding":"A systematic knockout screen in P. berghei showed that AP2-O has a non-essential function in blood stages (knockout does not block asexual replication), but AP2-O is required for normal ookinete development and mosquito transmission. Comparative transcriptomics across AP2-O mutants revealed clusters of co-regulated target genes with shared cis promoter elements.","method":"Systematic knockout screen of ApiAP2 genes in P. berghei; comparative transcriptomics of mutants across developmental stages","journal":"Cell host & microbe","confidence":"High","confidence_rationale":"Tier 2 / Strong — systematic KO screen with transcriptomics, multiple orthogonal analyses, independently consistent with prior work","pmids":["28081440"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2022,"finding":"AP2-Z, a newly identified AP2 transcription factor in P. berghei zygotes, has ChIP-seq target genes that largely overlap with AP2-O targets, suggesting AP2-O and AP2-Z function in a cascade of transcription factors beginning with AP2-G that proceeds from gametocytogenesis to ookinete formation. AP2-O target genes include the majority of genes known to be essential components of ookinetes.","method":"ChIP-seq of AP2-Z in P. berghei; comparison of AP2-Z target gene set with known AP2-O targets","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — ChIP-seq provides genome-wide binding data, but AP2-O's role here is inferred from overlap analysis rather than direct AP2-O experiment in this paper","pmids":["35947628"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"finding":"Deletion of the P. berghei NEK4 kinase gene causes reduced expression and phosphorylation of AP2-O, placing AP2-O downstream of NEK4 in the transcriptional and phosphoregulatory network governing meiosis and zygote maturation. This indicates AP2-O expression and activity are regulated post-translationally by NEK4-dependent phosphorylation.","method":"Transcriptomic and phosphoproteomic analyses of P. berghei nek4 deletion mutants; ultrastructure expansion microscopy","journal":"bioRxiv / Research square / Nature communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — AP2-O is one of many downstream effectors identified in a global proteomic/transcriptomic screen; the specific NEK4→AP2-O phosphorylation link is inferred from multi-protein datasets, not validated for AP2-O directly","pmids":["41332573","41510253","42120403"],"is_preprint":true,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"AP2-O (PF3D7_1143100) is an ApiAP2 family transcription factor whose mRNA is stored translationally repressed in female gametocytes and translated during ookinete development, where it activates ookinete-specific genes—including all genes required for mosquito midgut invasion—by binding specific six-base DNA motifs in proximal promoters; its DNA-binding domain determines target specificity (shown by domain-swap), it is non-essential in asexual blood stages, and it functions within a sequential transcription factor cascade (downstream of AP2-G and upstream of or parallel to AP2-Z) that controls sexual development from gametocytogenesis through ookinete formation."},"narrative":{"mechanistic_narrative":"AP2-O (PF3D7_1143100) is an ApiAP2-family transcription factor that drives the ookinete gene expression program required for Plasmodium transmission through the mosquito [PMID:19220746]. Its mRNA is pre-synthesized and held translationally repressed in intraerythrocytic female gametocytes, then translated during ookinete development, where AP2-O activates a set of genes needed for mosquito midgut invasion by binding specific six-base sequences in proximal promoters [PMID:19220746]. Target specificity is dictated by its AP2 DNA-binding domain: swapping this domain for that of AP2-Sp redirects AP2-O to induce AP2-Sp target genes in the ookinete stage, establishing the DNA-binding domain as the determinant of target choice within a swappable activation function [PMID:20025671]. AP2-O is dispensable for asexual blood-stage replication but required for normal ookinete development and transmission, and its targets fall into clusters of co-regulated genes sharing cis promoter elements [PMID:28081440]. Its regulon overlaps extensively with that of the zygote factor AP2-Z, consistent with AP2-O acting within a sequential ApiAP2 cascade—beginning with AP2-G—that spans gametocytogenesis to ookinete formation, and its targets include the majority of essential ookinete components [PMID:35947628]. All direct functional evidence derives from the P. berghei ortholog; no AP2-O-specific characterization in P. falciparum is present in the available corpus.","teleology":[{"year":2009,"claim":"Established that AP2-O is the transcriptional activator of the ookinete invasion program and revealed how its activity is timed—unknown before was what controls ookinete-specific gene expression and how a blood-stage-synthesized factor acts only later.","evidence":"Genetic disruption, transcriptomics, promoter-binding assays and stage-specific expression profiling in P. berghei","pmids":["19220746"],"confidence":"High","gaps":["Precise genome-wide binding sites not mapped by direct AP2-O ChIP","Molecular mechanism of translational repression/derepression not defined","No P. falciparum data"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2009,"claim":"Resolved whether target specificity resides in the DNA-binding domain versus other regions—the domain-swap showed the AP2 domain alone dictates which genes AP2-O activates.","evidence":"Domain-swap transgenic in P. berghei replacing the AP2-O DNA-binding domain with that of AP2-Sp, with target gene readout","pmids":["20025671"],"confidence":"High","gaps":["Does not identify cofactors or activation-domain partners","Structural basis of six-base motif recognition not determined"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2017,"claim":"Placed AP2-O in the broader stage-specificity of the ApiAP2 family—clarifying that it is non-essential in blood stages but required for ookinete development and transmission, and that its targets are organized into cis-element-defined regulons.","evidence":"Systematic ApiAP2 knockout screen with comparative developmental transcriptomics in P. berghei","pmids":["28081440"],"confidence":"High","gaps":["Co-regulated clusters not experimentally tied to direct AP2-O occupancy","Functional hierarchy among cluster genes unresolved"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2022,"claim":"Situated AP2-O within a sequential transcription-factor cascade by showing extensive overlap between AP2-O targets and the ChIP-seq regulon of the zygote factor AP2-Z, linking the program to upstream AP2-G.","evidence":"ChIP-seq of AP2-Z in P. berghei and comparison with the known AP2-O target set","pmids":["35947628"],"confidence":"Medium","gaps":["AP2-O's role inferred from target overlap, not a direct AP2-O experiment in this study","Whether AP2-O and AP2-Z act sequentially, in parallel, or combinatorially is unresolved"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"claim":"Began to address post-translational control of AP2-O, indicating its expression and phosphorylation depend on the NEK4 kinase within the meiosis/zygote-maturation network.","evidence":"Transcriptomic and phosphoproteomic profiling of P. berghei nek4 deletion mutants with expansion microscopy (preprint)","pmids":["41332573","41510253","42120403"],"confidence":"Low","gaps":["NEK4→AP2-O link inferred from multi-protein screens, not validated for AP2-O directly","Specific phosphosites on AP2-O and their functional consequences not defined","Direct versus indirect regulation by NEK4 not distinguished"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"Whether the AP2-O functions defined in P. berghei apply to P. falciparum PF3D7_1143100, and the identity of AP2-O's protein cofactors and its direct genome-wide binding sites, remain open.","evidence":"No direct AP2-O ChIP or interactome, and no P. falciparum functional study, is present in the corpus","pmids":[],"confidence":"Low","gaps":["No P. falciparum AP2-O experimental data","No direct AP2-O ChIP-seq occupancy map","No characterized protein interaction partners"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["AP2-Z (TARGET REGULON OVERLAP) [#3]","AP2-G (UPSTREAM IN CASCADE) [#3]","NEK4 KINASE (UPSTREAM PHOSPHOREGULATOR, LOW CONFIDENCE) [#4]"],"other_free_text":["mRNA translationally repressed in female gametocytes and translated during ookinete development [#0]","Activates genes required for mosquito midgut invasion via six-base proximal-promoter motifs [#0]","AP2 DNA-binding domain determines target specificity (domain-swap with AP2-Sp) [#1]","Non-essential in asexual blood stages; required for ookinete development and mosquito transmission [#2]","All functional evidence is from the P. berghei ortholog; no P. falciparum-specific data in the corpus [#0, #1, #2, #3]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"16040597","id":"PMC_16040597","title":"Discovery of the principal specific transcription factors of Apicomplexa and their implication for the 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square","url":"https://pubmed.ncbi.nlm.nih.gov/41510253","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AP2-O","AP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":27174,"output_tokens":1588,"usd":0.052671,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6648,"output_tokens":2731,"usd":0.050757,"stage2_stop_reason":"end_turn"},"total_usd":0.103428,"stage1_batch_id":"msgbatch_011BPjN2BL33F8cJXD1r7V7T","stage2_batch_id":"msgbatch_01Qwg6vP7qxkpL6Gsc7tajsb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2009,\n      \"finding\": \"AP2-O (PF3D7_1143100) was identified as a transcription factor that activates gene expression in ookinetes, the mosquito-invasive form of Plasmodium. Its mRNA is pre-synthesized by intraerythrocytic female gametocytes and translationally repressed, then translated during ookinete development. AP2-O activates a set of genes required for mosquito midgut invasion by binding to specific six-base sequences on proximal promoters.\",\n      \"method\": \"Genetic disruption of AP2-O, transcriptome analysis, promoter binding assays, and stage-specific expression profiling in Plasmodium berghei\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — loss-of-function with defined cellular and transcriptional phenotype, DNA-binding specificity demonstrated, translational regulation characterized; foundational study replicated by subsequent work\",\n      \"pmids\": [\"19220746\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2009,\n      \"finding\": \"In transgenic P. berghei parasites expressing endogenous AP2-O but with its AP2 DNA-binding domain swapped with that of AP2-Sp, several AP2-Sp target genes were induced in the ookinete stage, demonstrating that the DNA-binding domain of AP2-O determines target gene specificity and that AP2-O's transcriptional activation function is domain-swappable.\",\n      \"method\": \"Domain-swap transgenic experiment in P. berghei: AP2-O coding region with AP2-Sp DNA-binding domain inserted; target gene expression analyzed\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1 / Moderate — direct domain-swap mutagenesis with functional readout in vivo, single lab but mechanistically rigorous\",\n      \"pmids\": [\"20025671\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"finding\": \"A systematic knockout screen in P. berghei showed that AP2-O has a non-essential function in blood stages (knockout does not block asexual replication), but AP2-O is required for normal ookinete development and mosquito transmission. Comparative transcriptomics across AP2-O mutants revealed clusters of co-regulated target genes with shared cis promoter elements.\",\n      \"method\": \"Systematic knockout screen of ApiAP2 genes in P. berghei; comparative transcriptomics of mutants across developmental stages\",\n      \"journal\": \"Cell host & microbe\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — systematic KO screen with transcriptomics, multiple orthogonal analyses, independently consistent with prior work\",\n      \"pmids\": [\"28081440\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"AP2-Z, a newly identified AP2 transcription factor in P. berghei zygotes, has ChIP-seq target genes that largely overlap with AP2-O targets, suggesting AP2-O and AP2-Z function in a cascade of transcription factors beginning with AP2-G that proceeds from gametocytogenesis to ookinete formation. AP2-O target genes include the majority of genes known to be essential components of ookinetes.\",\n      \"method\": \"ChIP-seq of AP2-Z in P. berghei; comparison of AP2-Z target gene set with known AP2-O targets\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — ChIP-seq provides genome-wide binding data, but AP2-O's role here is inferred from overlap analysis rather than direct AP2-O experiment in this paper\",\n      \"pmids\": [\"35947628\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"Deletion of the P. berghei NEK4 kinase gene causes reduced expression and phosphorylation of AP2-O, placing AP2-O downstream of NEK4 in the transcriptional and phosphoregulatory network governing meiosis and zygote maturation. This indicates AP2-O expression and activity are regulated post-translationally by NEK4-dependent phosphorylation.\",\n      \"method\": \"Transcriptomic and phosphoproteomic analyses of P. berghei nek4 deletion mutants; ultrastructure expansion microscopy\",\n      \"journal\": \"bioRxiv / Research square / Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — AP2-O is one of many downstream effectors identified in a global proteomic/transcriptomic screen; the specific NEK4→AP2-O phosphorylation link is inferred from multi-protein datasets, not validated for AP2-O directly\",\n      \"pmids\": [\"41332573\", \"41510253\", \"42120403\"],\n      \"is_preprint\": true,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"AP2-O (PF3D7_1143100) is an ApiAP2 family transcription factor whose mRNA is stored translationally repressed in female gametocytes and translated during ookinete development, where it activates ookinete-specific genes—including all genes required for mosquito midgut invasion—by binding specific six-base DNA motifs in proximal promoters; its DNA-binding domain determines target specificity (shown by domain-swap), it is non-essential in asexual blood stages, and it functions within a sequential transcription factor cascade (downstream of AP2-G and upstream of or parallel to AP2-Z) that controls sexual development from gametocytogenesis through ookinete formation.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AP2-O (PF3D7_1143100) is an ApiAP2-family transcription factor that drives the ookinete gene expression program required for Plasmodium transmission through the mosquito [#0]. Its mRNA is pre-synthesized and held translationally repressed in intraerythrocytic female gametocytes, then translated during ookinete development, where AP2-O activates a set of genes needed for mosquito midgut invasion by binding specific six-base sequences in proximal promoters [#0]. Target specificity is dictated by its AP2 DNA-binding domain: swapping this domain for that of AP2-Sp redirects AP2-O to induce AP2-Sp target genes in the ookinete stage, establishing the DNA-binding domain as the determinant of target choice within a swappable activation function [#1]. AP2-O is dispensable for asexual blood-stage replication but required for normal ookinete development and transmission, and its targets fall into clusters of co-regulated genes sharing cis promoter elements [#2]. Its regulon overlaps extensively with that of the zygote factor AP2-Z, consistent with AP2-O acting within a sequential ApiAP2 cascade—beginning with AP2-G—that spans gametocytogenesis to ookinete formation, and its targets include the majority of essential ookinete components [#3]. All direct functional evidence derives from the P. berghei ortholog; no AP2-O-specific characterization in P. falciparum is present in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2009,\n      \"claim\": \"Established that AP2-O is the transcriptional activator of the ookinete invasion program and revealed how its activity is timed—unknown before was what controls ookinete-specific gene expression and how a blood-stage-synthesized factor acts only later.\",\n      \"evidence\": \"Genetic disruption, transcriptomics, promoter-binding assays and stage-specific expression profiling in P. berghei\",\n      \"pmids\": [\"19220746\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Precise genome-wide binding sites not mapped by direct AP2-O ChIP\", \"Molecular mechanism of translational repression/derepression not defined\", \"No P. falciparum data\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2009,\n      \"claim\": \"Resolved whether target specificity resides in the DNA-binding domain versus other regions—the domain-swap showed the AP2 domain alone dictates which genes AP2-O activates.\",\n      \"evidence\": \"Domain-swap transgenic in P. berghei replacing the AP2-O DNA-binding domain with that of AP2-Sp, with target gene readout\",\n      \"pmids\": [\"20025671\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Does not identify cofactors or activation-domain partners\", \"Structural basis of six-base motif recognition not determined\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"claim\": \"Placed AP2-O in the broader stage-specificity of the ApiAP2 family—clarifying that it is non-essential in blood stages but required for ookinete development and transmission, and that its targets are organized into cis-element-defined regulons.\",\n      \"evidence\": \"Systematic ApiAP2 knockout screen with comparative developmental transcriptomics in P. berghei\",\n      \"pmids\": [\"28081440\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Co-regulated clusters not experimentally tied to direct AP2-O occupancy\", \"Functional hierarchy among cluster genes unresolved\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"Situated AP2-O within a sequential transcription-factor cascade by showing extensive overlap between AP2-O targets and the ChIP-seq regulon of the zygote factor AP2-Z, linking the program to upstream AP2-G.\",\n      \"evidence\": \"ChIP-seq of AP2-Z in P. berghei and comparison with the known AP2-O target set\",\n      \"pmids\": [\"35947628\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"AP2-O's role inferred from target overlap, not a direct AP2-O experiment in this study\", \"Whether AP2-O and AP2-Z act sequentially, in parallel, or combinatorially is unresolved\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Began to address post-translational control of AP2-O, indicating its expression and phosphorylation depend on the NEK4 kinase within the meiosis/zygote-maturation network.\",\n      \"evidence\": \"Transcriptomic and phosphoproteomic profiling of P. berghei nek4 deletion mutants with expansion microscopy (preprint)\",\n      \"pmids\": [\"41332573\", \"41510253\", \"42120403\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"NEK4\\u2192AP2-O link inferred from multi-protein screens, not validated for AP2-O directly\", \"Specific phosphosites on AP2-O and their functional consequences not defined\", \"Direct versus indirect regulation by NEK4 not distinguished\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether the AP2-O functions defined in P. berghei apply to P. falciparum PF3D7_1143100, and the identity of AP2-O's protein cofactors and its direct genome-wide binding sites, remain open.\",\n      \"evidence\": \"No direct AP2-O ChIP or interactome, and no P. falciparum functional study, is present in the corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No P. falciparum AP2-O experimental data\", \"No direct AP2-O ChIP-seq occupancy map\", \"No characterized protein interaction partners\"],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003700\", \"supporting_discovery_ids\": [0, 1, 2]},\n      {\"term_id\": \"GO:0000981\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0043565\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0045893\", \"supporting_discovery_ids\": [0, 2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0060964\", \"supporting_discovery_ids\": [0, 2, 3]},\n      {\"term_id\": \"GO:0090630\", \"supporting_discovery_ids\": [4]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\n      \"AP2-Z (target regulon overlap) [#3]\",\n      \"AP2-G (upstream in cascade) [#3]\",\n      \"NEK4 kinase (upstream phosphoregulator, Low confidence) [#4]\"\n    ],\n    \"other_free_text\": [\n      \"mRNA translationally repressed in female gametocytes and translated during ookinete development [#0]\",\n      \"Activates genes required for mosquito midgut invasion via six-base proximal-promoter motifs [#0]\",\n      \"AP2 DNA-binding domain determines target specificity (domain-swap with AP2-Sp) [#1]\",\n      \"Non-essential in asexual blood stages; required for ookinete development and mosquito transmission [#2]\",\n      \"All functional evidence is from the P. berghei ortholog; no P. falciparum-specific data in the corpus [#0, #1, #2, #3]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1143100","name":null,"product":"AP2 domain transcription factor AP2-O","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0905900","name":null,"product":"AP2 domain transcription factor AP2-O","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0941000","name":null,"product":"AP2 domain transcription factor AP2-O, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AP2-O","evaluation":null}