{"gene":"PF3D7_1126100","run_date":"2026-09-08T12:51:28","timeline":{"discoveries":[{"year":2013,"finding":"PbATG7 (ATG7 ortholog in P. berghei) is co-transcribed with PbATG3 and PbATG8 across all parasitic stages, consistent with its role as the E1-type activating enzyme in the ATG8 conjugation pathway.","method":"Transcriptional analysis of ATG gene expression across life-cycle stages","journal":"Autophagy","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single lab, co-transcription data only, no direct enzymatic or functional validation of ATG7 activity in this study","pmids":["24342964"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""},{"species":"Plasmodium falciparum","strain":""}]},{"year":2013,"finding":"PfATG7 (PF3D7_1126100) is expressed during erythrocytic stages as two distinct protein species (~150 kDa and ~65 kDa, or ~200 kDa and ~100 kDa depending on the tag), and conditional attenuation of PfATG7 protein levels causes a slow growth phenotype, demonstrating that PfATG7 is essential for normal erythrocytic-stage parasite growth.","method":"C-terminal HA tagging of endogenous PfATG7 locus (western blot); inducible degradation-domain knock-in for conditional protein depletion; growth assay","journal":"PloS one","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — conditional genetic knockdown with defined growth phenotype, two independent transgenic lines, single lab","pmids":["23825614"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"finding":"P. berghei ATG7 is an E1-like enzyme that is essential for ATG8 lipidation (membrane conjugation); depletion of ATG7 abolishes ATG8 lipidation, causes loss of ATG8 localization on the apicoplast membrane, impairs microneme exocytosis, disrupts organelle biogenesis, and prevents merozoite formation during liver-stage development. ATG7 localizes to the parasite cytosol across blood, sporozoite, and liver stages.","method":"Conditional mutagenesis (ATG7 depletion) in P. berghei; western blot for ATG8 lipidation; fluorescence microscopy for ATG8 localization and organelle markers; functional assays for microneme exocytosis and merozoite formation","journal":"mBio","confidence":"High","confidence_rationale":"Tier 2 / Strong — conditional genetic depletion with multiple orthogonal readouts (lipidation assay, localization, organelle biogenesis, cell development), clearly establishes ATG7 as the E1-like activator upstream of ATG8 lipidation","pmids":["39714137"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"finding":"Cytosolic ATG7 in P. berghei is required for ATG8 membrane localization and lipidation during liver-stage parasite metamorphosis; parasites lacking ATG7 fail to lipidate ATG8, exhibit impaired microneme exocytosis, and fail to mature into hepatic merozoites, establishing ATG7 as essential for cellular homeostasis during liver-stage development.","method":"Conditional mutagenesis system (ATG7 depletion) in P. berghei liver-stage parasites; functional assays for ATG8 lipidation, microneme exocytosis, and merozoite maturation","journal":"Autophagy reports","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — conditional knockdown with defined functional readouts; overlaps with PMID:39714137 by the same lab, providing partial replication but not fully independent","pmids":["40395990"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"PF3D7_1126100 (ATG7) encodes an E1-like activating enzyme that is essential for ATG8 lipidation and membrane conjugation in Plasmodium; it localizes to the parasite cytosol, and its depletion abolishes ATG8 association with the apicoplast membrane, impairs organelle biogenesis and microneme exocytosis, and blocks merozoite formation, demonstrating that ATG7 is required for normal parasite development across blood and liver stages."},"narrative":{"mechanistic_narrative":"PF3D7_1126100 (ATG7) encodes an E1-like activating enzyme that operates at the apex of the ATG8 conjugation pathway in Plasmodium, driving ATG8 lipidation and membrane association during parasite development [PMID:39714137]. In P. berghei, conditional depletion of ATG7 abolishes ATG8 lipidation and eliminates ATG8 localization to the apicoplast membrane, which in turn disrupts organelle biogenesis, impairs microneme exocytosis, and prevents merozoite formation during liver-stage metamorphosis; ATG7 itself resides in the parasite cytosol across blood, sporozoite, and liver stages [PMID:39714137, PMID:40395990]. In P. falciparum, endogenously tagged PfATG7 is expressed as distinct protein species during erythrocytic stages, and conditional attenuation of its protein level produces a slow-growth phenotype, establishing that PfATG7 is required for normal blood-stage growth [PMID:23825614]. Beyond its role as the E1-like activator upstream of ATG8 lipidation, no further catalytic mechanism or direct enzymatic characterization of PF3D7_1126100 has been established in the available corpus.","teleology":[{"year":2013,"claim":"Before functional data existed, it was unknown whether the parasite ATG7 ortholog was engaged in the ATG8 conjugation system at all; co-expression analysis placed it within that pathway.","evidence":"Transcriptional analysis of ATG gene expression across P. berghei life-cycle stages, showing co-transcription with ATG3 and ATG8","pmids":["24342964"],"confidence":"Low","gaps":["Co-transcription only; no direct enzymatic or functional validation of ATG7 activity","Does not demonstrate physical or biochemical interaction with ATG8/ATG3","No P. falciparum-specific data in this study"],"attribution":[{"species":"Plasmodium berghei","strain":""},{"species":"Plasmodium falciparum","strain":""}]},{"year":2013,"claim":"It was unknown whether PfATG7 is required for parasite viability; conditional protein depletion established that it is essential for normal erythrocytic-stage growth.","evidence":"C-terminal HA tagging of the endogenous PfATG7 locus with western blot detection and an inducible degradation-domain knock-in for conditional depletion, coupled to growth assays in P. falciparum","pmids":["23825614"],"confidence":"Medium","gaps":["Growth phenotype does not identify the molecular defect underlying slow growth","No demonstration of ATG8 lipidation or enzymatic activity in P. falciparum","Identity of the two protein species and their processing is unresolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"claim":"The catalytic role of ATG7 was undefined; conditional depletion showed it is the E1-like activator required for ATG8 lipidation and membrane conjugation, linking it to apicoplast biology and organelle biogenesis.","evidence":"Conditional mutagenesis (ATG7 depletion) in P. berghei with western blot for ATG8 lipidation, fluorescence microscopy for ATG8 and organelle markers, and functional assays for microneme exocytosis and merozoite formation","pmids":["39714137"],"confidence":"High","gaps":["Direct in vitro reconstitution of ATG7 E1 activity not shown","Mechanism connecting loss of ATG8 lipidation to microneme exocytosis defect not resolved","Cytosolic localization does not explain how ATG7 output reaches the apicoplast membrane"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"claim":"It was unclear whether ATG7 function extends to liver-stage metamorphosis; depletion during liver-stage development confirmed it is required for ATG8 lipidation, microneme exocytosis, and maturation into hepatic merozoites.","evidence":"Conditional mutagenesis system (ATG7 depletion) in P. berghei liver-stage parasites with functional assays for ATG8 lipidation, microneme exocytosis, and merozoite maturation","pmids":["40395990"],"confidence":"Medium","gaps":["Overlaps with and only partially replicates the same lab's prior work rather than independent confirmation","Does not resolve the downstream homeostatic target of ATG8 lipidation in liver stage"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"Whether PF3D7_1126100 possesses direct, biochemically reconstituted E1 activity and how its cytosolic activity is spatially coupled to ATG8 membrane conjugation in P. falciparum remain unresolved.","evidence":"No timeline discovery provides in vitro enzymatic reconstitution or a mechanistic model of cytosol-to-membrane coupling","pmids":[],"confidence":"Low","gaps":["No in vitro demonstration of ATG7 adenylation/thioester chemistry","No structural model of PF3D7_1126100","P. falciparum functional data limited to a growth phenotype without lipidation readout"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["ATG8 (SUBSTRATE OF ATG7-DEPENDENT LIPIDATION)","ATG3 (CO-TRANSCRIBED ATG8-CONJUGATION PATHWAY COMPONENT)"],"other_free_text":["Depletion in P. berghei abolishes ATG8 lipidation and ATG8 localization to the apicoplast membrane [#2].","ATG7 loss impairs microneme exocytosis, disrupts organelle biogenesis, and blocks merozoite formation in liver stage [#2, #3].","Conditional attenuation of PfATG7 in P. falciparum produces a slow-growth phenotype during erythrocytic stages [#1].","PfATG7 is detected as two distinct protein species during erythrocytic stages [#1]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"22900071","id":"PMC_22900071","title":"Autophagy-related Atg8 localizes to the apicoplast of the human malaria parasite Plasmodium falciparum.","date":"2012","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/22900071","citation_count":69,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ATG7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"23388496","id":"PMC_23388496","title":"Features of autophagic cell death in Plasmodium liver-stage parasites.","date":"2013","source":"Autophagy","url":"https://pubmed.ncbi.nlm.nih.gov/23388496","citation_count":56,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ATG7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"24342964","id":"PMC_24342964","title":"Characterization of the ATG8-conjugation system in 2 Plasmodium species with special focus on the liver stage: possible linkage between the apicoplastic and autophagic systems?","date":"2013","source":"Autophagy","url":"https://pubmed.ncbi.nlm.nih.gov/24342964","citation_count":36,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PbATG7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"30726238","id":"PMC_30726238","title":"A mutagenesis screen for essential plastid biogenesis genes in human malaria parasites.","date":"2019","source":"PLoS biology","url":"https://pubmed.ncbi.nlm.nih.gov/30726238","citation_count":35,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"23825614","id":"PMC_23825614","title":"Plasmodium falciparum erythrocytic stage parasites require the putative autophagy protein PfAtg7 for normal growth.","date":"2013","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/23825614","citation_count":26,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ATG7","PfATG7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39714137","id":"PMC_39714137","title":"Autophagy protein Atg7 is essential for maintaining malaria parasite cellular homeostasis and organelle biogenesis.","date":"2024","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/39714137","citation_count":10,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ATG7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40395990","id":"PMC_40395990","title":"Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy.","date":"2025","source":"Autophagy reports","url":"https://pubmed.ncbi.nlm.nih.gov/40395990","citation_count":2,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ATG7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42473197","id":"PMC_42473197","title":"Divergent Autophagy Pathways in Plasmodium: Mechanisms, Functions, and Therapeutic Potential.","date":"2026","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/42473197","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ATG7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":6677,"output_tokens":1329,"usd":0.019983,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6175,"output_tokens":2536,"usd":0.047137,"stage2_stop_reason":"end_turn"},"total_usd":0.06712,"stage1_batch_id":"msgbatch_01DisAaTypwmC8QwdMBuePHo","stage2_batch_id":"msgbatch_0184zxZTqxiEfdYYfPMfkqjb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2013,\n      \"finding\": \"PbATG7 (ATG7 ortholog in P. berghei) is co-transcribed with PbATG3 and PbATG8 across all parasitic stages, consistent with its role as the E1-type activating enzyme in the ATG8 conjugation pathway.\",\n      \"method\": \"Transcriptional analysis of ATG gene expression across life-cycle stages\",\n      \"journal\": \"Autophagy\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab, co-transcription data only, no direct enzymatic or functional validation of ATG7 activity in this study\",\n      \"pmids\": [\"24342964\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2013,\n      \"finding\": \"PfATG7 (PF3D7_1126100) is expressed during erythrocytic stages as two distinct protein species (~150 kDa and ~65 kDa, or ~200 kDa and ~100 kDa depending on the tag), and conditional attenuation of PfATG7 protein levels causes a slow growth phenotype, demonstrating that PfATG7 is essential for normal erythrocytic-stage parasite growth.\",\n      \"method\": \"C-terminal HA tagging of endogenous PfATG7 locus (western blot); inducible degradation-domain knock-in for conditional protein depletion; growth assay\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — conditional genetic knockdown with defined growth phenotype, two independent transgenic lines, single lab\",\n      \"pmids\": [\"23825614\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"P. berghei ATG7 is an E1-like enzyme that is essential for ATG8 lipidation (membrane conjugation); depletion of ATG7 abolishes ATG8 lipidation, causes loss of ATG8 localization on the apicoplast membrane, impairs microneme exocytosis, disrupts organelle biogenesis, and prevents merozoite formation during liver-stage development. ATG7 localizes to the parasite cytosol across blood, sporozoite, and liver stages.\",\n      \"method\": \"Conditional mutagenesis (ATG7 depletion) in P. berghei; western blot for ATG8 lipidation; fluorescence microscopy for ATG8 localization and organelle markers; functional assays for microneme exocytosis and merozoite formation\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — conditional genetic depletion with multiple orthogonal readouts (lipidation assay, localization, organelle biogenesis, cell development), clearly establishes ATG7 as the E1-like activator upstream of ATG8 lipidation\",\n      \"pmids\": [\"39714137\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"Cytosolic ATG7 in P. berghei is required for ATG8 membrane localization and lipidation during liver-stage parasite metamorphosis; parasites lacking ATG7 fail to lipidate ATG8, exhibit impaired microneme exocytosis, and fail to mature into hepatic merozoites, establishing ATG7 as essential for cellular homeostasis during liver-stage development.\",\n      \"method\": \"Conditional mutagenesis system (ATG7 depletion) in P. berghei liver-stage parasites; functional assays for ATG8 lipidation, microneme exocytosis, and merozoite maturation\",\n      \"journal\": \"Autophagy reports\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — conditional knockdown with defined functional readouts; overlaps with PMID:39714137 by the same lab, providing partial replication but not fully independent\",\n      \"pmids\": [\"40395990\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_1126100 (ATG7) encodes an E1-like activating enzyme that is essential for ATG8 lipidation and membrane conjugation in Plasmodium; it localizes to the parasite cytosol, and its depletion abolishes ATG8 association with the apicoplast membrane, impairs organelle biogenesis and microneme exocytosis, and blocks merozoite formation, demonstrating that ATG7 is required for normal parasite development across blood and liver stages.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_1126100 (ATG7) encodes an E1-like activating enzyme that operates at the apex of the ATG8 conjugation pathway in Plasmodium, driving ATG8 lipidation and membrane association during parasite development [#2]. In P. berghei, conditional depletion of ATG7 abolishes ATG8 lipidation and eliminates ATG8 localization to the apicoplast membrane, which in turn disrupts organelle biogenesis, impairs microneme exocytosis, and prevents merozoite formation during liver-stage metamorphosis; ATG7 itself resides in the parasite cytosol across blood, sporozoite, and liver stages [#2, #3]. In P. falciparum, endogenously tagged PfATG7 is expressed as distinct protein species during erythrocytic stages, and conditional attenuation of its protein level produces a slow-growth phenotype, establishing that PfATG7 is required for normal blood-stage growth [#1]. Beyond its role as the E1-like activator upstream of ATG8 lipidation, no further catalytic mechanism or direct enzymatic characterization of PF3D7_1126100 has been established in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2013,\n      \"claim\": \"Before functional data existed, it was unknown whether the parasite ATG7 ortholog was engaged in the ATG8 conjugation system at all; co-expression analysis placed it within that pathway.\",\n      \"evidence\": \"Transcriptional analysis of ATG gene expression across P. berghei life-cycle stages, showing co-transcription with ATG3 and ATG8\",\n      \"pmids\": [\"24342964\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Co-transcription only; no direct enzymatic or functional validation of ATG7 activity\",\n        \"Does not demonstrate physical or biochemical interaction with ATG8/ATG3\",\n        \"No P. falciparum-specific data in this study\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2013,\n      \"claim\": \"It was unknown whether PfATG7 is required for parasite viability; conditional protein depletion established that it is essential for normal erythrocytic-stage growth.\",\n      \"evidence\": \"C-terminal HA tagging of the endogenous PfATG7 locus with western blot detection and an inducible degradation-domain knock-in for conditional depletion, coupled to growth assays in P. falciparum\",\n      \"pmids\": [\"23825614\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Growth phenotype does not identify the molecular defect underlying slow growth\",\n        \"No demonstration of ATG8 lipidation or enzymatic activity in P. falciparum\",\n        \"Identity of the two protein species and their processing is unresolved\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"The catalytic role of ATG7 was undefined; conditional depletion showed it is the E1-like activator required for ATG8 lipidation and membrane conjugation, linking it to apicoplast biology and organelle biogenesis.\",\n      \"evidence\": \"Conditional mutagenesis (ATG7 depletion) in P. berghei with western blot for ATG8 lipidation, fluorescence microscopy for ATG8 and organelle markers, and functional assays for microneme exocytosis and merozoite formation\",\n      \"pmids\": [\"39714137\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Direct in vitro reconstitution of ATG7 E1 activity not shown\",\n        \"Mechanism connecting loss of ATG8 lipidation to microneme exocytosis defect not resolved\",\n        \"Cytosolic localization does not explain how ATG7 output reaches the apicoplast membrane\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"It was unclear whether ATG7 function extends to liver-stage metamorphosis; depletion during liver-stage development confirmed it is required for ATG8 lipidation, microneme exocytosis, and maturation into hepatic merozoites.\",\n      \"evidence\": \"Conditional mutagenesis system (ATG7 depletion) in P. berghei liver-stage parasites with functional assays for ATG8 lipidation, microneme exocytosis, and merozoite maturation\",\n      \"pmids\": [\"40395990\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Overlaps with and only partially replicates the same lab's prior work rather than independent confirmation\",\n        \"Does not resolve the downstream homeostatic target of ATG8 lipidation in liver stage\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether PF3D7_1126100 possesses direct, biochemically reconstituted E1 activity and how its cytosolic activity is spatially coupled to ATG8 membrane conjugation in P. falciparum remain unresolved.\",\n      \"evidence\": \"No timeline discovery provides in vitro enzymatic reconstitution or a mechanistic model of cytosol-to-membrane coupling\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No in vitro demonstration of ATG7 adenylation/thioester chemistry\",\n        \"No structural model of PF3D7_1126100\",\n        \"P. falciparum functional data limited to a growth phenotype without lipidation readout\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0019778\", \"supporting_discovery_ids\": [2, 3]},\n      {\"term_id\": \"GO:0019776\", \"supporting_discovery_ids\": [0, 2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005829\", \"supporting_discovery_ids\": [2, 3]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006914\", \"supporting_discovery_ids\": [0, 2, 3]},\n      {\"term_id\": \"GO:0016240\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\n      \"ATG8 (substrate of ATG7-dependent lipidation)\",\n      \"ATG3 (co-transcribed ATG8-conjugation pathway component)\"\n    ],\n    \"other_free_text\": [\n      \"Depletion in P. berghei abolishes ATG8 lipidation and ATG8 localization to the apicoplast membrane [#2].\",\n      \"ATG7 loss impairs microneme exocytosis, disrupts organelle biogenesis, and blocks merozoite formation in liver stage [#2, #3].\",\n      \"Conditional attenuation of PfATG7 in P. falciparum produces a slow-growth phenotype during erythrocytic stages [#1].\",\n      \"PfATG7 is detected as two distinct protein species during erythrocytic stages [#1].\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1126100","name":null,"product":"autophagy-related protein 7, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0922200","name":null,"product":"autophagy-related protein 7, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0924000","name":null,"product":"autophagy-related protein 7, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"ATG7","evaluation":null}