{"gene":"PF3D7_1124300","run_date":"2026-09-08T19:44:17","timeline":{"discoveries":[{"year":2022,"finding":"BDP7 (PF3D7_1124300) is a nuclear protein that is a constitutive component of the P. falciparum BDP1/BDP2 core complex; it physically interacts with PfBDP1 at invasion gene promoters in mature schizont-stage parasites, as confirmed by co-immunoprecipitation and ChIP-seq, and structural modelling predicted a typical bromodomain fold.","method":"Co-immunoprecipitation, ChIP-seq, structural modelling, conditional knockdown (DiCre-based), transgenic parasite lines","journal":"Frontiers in cell and developmental biology","confidence":"High","confidence_rationale":"Tier 2 / Strong — reciprocal protein interaction confirmed, ChIP-seq genome-wide binding, conditional knockdown with defined phenotypic readout, single lab but multiple orthogonal methods","pmids":["35493110"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"finding":"Conditional knockdown of BDP7 (PF3D7_1124300) results in de-repression of variant surface antigen (VSA) families—including PfEMP1, RIFIN, STEVOR, and PfMC-2TM—at both the mRNA and protein level, establishing a direct role for BDP7 in VSA silencing.","method":"Conditional knockdown (DiCre-based), RT-qPCR, Western blot / flow cytometry for VSA protein levels","journal":"Frontiers in cell and developmental biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — conditional knockdown with two orthogonal readouts (mRNA and protein), single lab","pmids":["35493110"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"finding":"ChIP-seq mapping shows that BDP7 (PF3D7_1124300) and PfBDP1 are co-enriched in heterochromatic regions across the gene bodies of all VSA families in early schizonts, indicating that BDP7 contributes to VSA silencing by associating with heterochromatin.","method":"ChIP-seq","journal":"Frontiers in cell and developmental biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct genome-wide chromatin occupancy mapping, single lab","pmids":["35493110"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"finding":"Partial depletion of BDP7 (PF3D7_1124300) showed no significant effect on parasite viability, indicating BDP7 is not individually essential under standard growth conditions at the depletion levels achieved.","method":"Conditional knockdown (DiCre-based), growth assay","journal":"Frontiers in cell and developmental biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — negative result from conditional knockdown, single lab, partial depletion may limit interpretation","pmids":["35493110"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2012,"finding":"BDP7 (PF3D7_1124300) was identified as a nuclear protein in P. falciparum by high-accuracy mass spectrometry of an experimentally determined nuclear proteome, with nuclear localization validated by transgenic cell line imaging.","method":"Nuclear fractionation followed by mass spectrometry; validation by transgenic GFP-tagged cell lines and fluorescence microscopy","journal":"Genome biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — nuclear fractionation plus mass spectrometry plus transgenic fluorescence validation, but this is a proteome-wide study with limited gene-specific mechanistic depth","pmids":["23181666"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"BDP7 (PF3D7_1124300) is a nuclear bromodomain-containing protein in P. falciparum that constitutively associates with PfBDP1 and PfBDP2 as part of a core chromatin regulatory complex; it co-occupies heterochromatic regions across all variant surface antigen (VSA) gene families together with PfBDP1 in early schizonts, and its conditional depletion de-represses VSA expression at both the mRNA and protein levels, establishing BDP7 as a novel player in heterochromatin-mediated silencing of P. falciparum virulence gene families, while also contributing to invasion gene transcription at schizont-stage promoters."},"narrative":{"mechanistic_narrative":"BDP7 (PF3D7_1124300) is a nuclear bromodomain-containing protein of Plasmodium falciparum that functions in heterochromatin-mediated silencing of clonally variant virulence gene families [PMID:35493110, PMID:23181666]. It is a constitutive component of the PfBDP1/BDP2 core chromatin complex, physically associating with PfBDP1, and shares genome-wide chromatin occupancy with PfBDP1 both at invasion gene promoters in mature schizonts and across the gene bodies of variant surface antigen (VSA) families within heterochromatin in early schizonts [PMID:35493110]. Conditional depletion of BDP7 de-represses the PfEMP1, RIFIN, STEVOR, and PfMC-2TM VSA families at the mRNA and protein levels, establishing a direct role in maintaining their silencing [PMID:35493110]. Partial depletion does not detectably impair parasite viability under standard culture, indicating BDP7 is not individually essential at the depletion levels achieved [PMID:35493110]. Beyond its complex membership, chromatin association, and silencing phenotype, no further mechanistic detail—such as the histone modifications recognized by its predicted bromodomain—has been characterized in the available corpus.","teleology":[{"year":2012,"claim":"Before any functional annotation, establishing where BDP7 resides in the cell was the first step toward its role; this showed BDP7 is a nuclear protein, consistent with a chromatin-associated function.","evidence":"Nuclear fractionation and mass spectrometry of the P. falciparum nuclear proteome, validated by transgenic GFP-tagged fluorescence microscopy","pmids":["23181666"],"confidence":"Medium","gaps":["No functional or mechanistic role assigned in this study","No interaction partners or chromatin targets identified","Proteome-wide survey with limited gene-specific depth"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"The open question of whether BDP7 acts alone or within a complex was resolved by showing it constitutively associates with PfBDP1/BDP2 and co-occupies invasion gene promoters, placing BDP7 in a defined core chromatin regulatory complex.","evidence":"Co-immunoprecipitation and ChIP-seq in mature schizont-stage parasites, with bromodomain fold predicted by structural modelling","pmids":["35493110"],"confidence":"High","gaps":["Bromodomain ligand/histone mark recognized by BDP7 not experimentally determined","Stoichiometry and architecture of the BDP1/BDP2/BDP7 complex unresolved","Direct DNA/chromatin binding by BDP7 versus recruitment via PfBDP1 not distinguished"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"Whether BDP7 has a causal role in gene regulation was tested by depletion, showing it is required to keep the VSA virulence gene families silenced at both transcript and protein levels.","evidence":"DiCre-based conditional knockdown with RT-qPCR and Western blot/flow cytometry readouts of PfEMP1, RIFIN, STEVOR, and PfMC-2TM","pmids":["35493110"],"confidence":"Medium","gaps":["Whether BDP7 silencing acts through the bromodomain or another domain is untested","Direct versus indirect contribution to each VSA family not dissected","Single lab; partial depletion may underestimate effects"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"Mapping where BDP7 acts on the genome tied its silencing role to a physical location, showing co-enrichment with PfBDP1 in heterochromatin across VSA gene bodies in early schizonts.","evidence":"ChIP-seq chromatin occupancy mapping","pmids":["35493110"],"confidence":"Medium","gaps":["Histone modification landscape underlying BDP7 recruitment not defined","Temporal dynamics of occupancy across the cell cycle not fully resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"The question of essentiality was addressed, showing partial BDP7 depletion does not detectably affect viability, indicating BDP7 is not individually essential at the depletion achieved.","evidence":"DiCre-based conditional knockdown with growth assay","pmids":["35493110"],"confidence":"Medium","gaps":["Negative result limited by incomplete depletion","Possible essentiality under stress or in specific life-cycle stages untested","Redundancy with other complex members not evaluated"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"It remains unknown what chromatin mark BDP7's predicted bromodomain recognizes and whether that recognition is mechanistically required for its role in complex assembly, heterochromatin targeting, and VSA silencing.","evidence":"","pmids":[],"confidence":"Low","gaps":["No experimental demonstration of a histone-mark ligand for the BDP7 bromodomain","No structure of BDP7 or the intact BDP1/BDP2/BDP7 complex","Separation-of-function mutants distinguishing binding from silencing not reported"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["PfBDP1/BDP2 core chromatin complex (BDP7 as constitutive component) [#0]"],"partners":["PFBDP1 [#0, #2]","PFBDP2 (AS PART OF THE CORE COMPLEX) [#0]"],"other_free_text":["Conditional depletion de-represses VSA families PfEMP1, RIFIN, STEVOR, and PfMC-2TM at mRNA and protein levels [#1]","Co-occupies heterochromatin across VSA gene bodies with PfBDP1 in early schizonts [#2]","Occupies invasion gene promoters in mature schizonts [#0]","Not individually essential for viability at partial depletion under standard growth [#3]","Predicted typical bromodomain fold by structural modelling; ligand not experimentally determined [#0]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"23181666","id":"PMC_23181666","title":"Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum.","date":"2012","source":"Genome biology","url":"https://pubmed.ncbi.nlm.nih.gov/23181666","citation_count":120,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35493110","id":"PMC_35493110","title":"The Putative Bromodomain Protein PfBDP7 of the Human Malaria Parasite Plasmodium Falciparum Cooperates With PfBDP1 in the Silencing of Variant Surface Antigen Expression.","date":"2022","source":"Frontiers in cell and developmental biology","url":"https://pubmed.ncbi.nlm.nih.gov/35493110","citation_count":10,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfBDP7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42140435","id":"","title":"Parasite-specific essential bromodomain protein TgBDP4 is a key epigenetic reader and a potential drug target for the parasite Toxoplasma gondii.","date":"2026","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/42140435","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["BDP7"],"competing_organism_flag":true,"competing_organism_match":"Toxoplasma","competing_organism_only":true},{"pmid":"37630530","id":"","title":"Plasmodium falciparum Development from Gametocyte to Oocyst: Insight from Functional Studies.","date":"2023","source":"Microorganisms","url":"https://pubmed.ncbi.nlm.nih.gov/37630530","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["BDP7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37882786","id":"","title":"The P. falciparum alternative histones Pf H2A.Z and Pf H2B.Z are dynamically acetylated and antagonized by PfSir2 histone deacetylases at heterochromatin boundaries.","date":"2023","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/37882786","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["BDP7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36328269","id":"","title":"Structural insights into acetylated histone ligand recognition by the BDP1 bromodomain of Plasmodium falciparum.","date":"2022","source":"International journal of biological macromolecules","url":"https://pubmed.ncbi.nlm.nih.gov/36328269","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["BDP7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37350586","id":"","title":"An apicomplexan bromodomain protein, TgBDP1, associates with diverse epigenetic factors to regulate essential transcriptional processes in Toxoplasma gondii.","date":"2023","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/37350586","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["BDP7"],"competing_organism_flag":true,"competing_organism_match":"Toxoplasma","competing_organism_only":true}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":8958,"output_tokens":1364,"usd":0.023667,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6328,"output_tokens":2629,"usd":0.048682,"stage2_stop_reason":"end_turn"},"total_usd":0.072349,"stage1_batch_id":"msgbatch_01SRp5hyRc82zNjddxyCCXGk","stage2_batch_id":"msgbatch_01K3BTmBazRo3pRrMvyaPMTY","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"BDP7 (PF3D7_1124300) is a nuclear protein that is a constitutive component of the P. falciparum BDP1/BDP2 core complex; it physically interacts with PfBDP1 at invasion gene promoters in mature schizont-stage parasites, as confirmed by co-immunoprecipitation and ChIP-seq, and structural modelling predicted a typical bromodomain fold.\",\n      \"method\": \"Co-immunoprecipitation, ChIP-seq, structural modelling, conditional knockdown (DiCre-based), transgenic parasite lines\",\n      \"journal\": \"Frontiers in cell and developmental biology\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — reciprocal protein interaction confirmed, ChIP-seq genome-wide binding, conditional knockdown with defined phenotypic readout, single lab but multiple orthogonal methods\",\n      \"pmids\": [\"35493110\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"Conditional knockdown of BDP7 (PF3D7_1124300) results in de-repression of variant surface antigen (VSA) families—including PfEMP1, RIFIN, STEVOR, and PfMC-2TM—at both the mRNA and protein level, establishing a direct role for BDP7 in VSA silencing.\",\n      \"method\": \"Conditional knockdown (DiCre-based), RT-qPCR, Western blot / flow cytometry for VSA protein levels\",\n      \"journal\": \"Frontiers in cell and developmental biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — conditional knockdown with two orthogonal readouts (mRNA and protein), single lab\",\n      \"pmids\": [\"35493110\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"ChIP-seq mapping shows that BDP7 (PF3D7_1124300) and PfBDP1 are co-enriched in heterochromatic regions across the gene bodies of all VSA families in early schizonts, indicating that BDP7 contributes to VSA silencing by associating with heterochromatin.\",\n      \"method\": \"ChIP-seq\",\n      \"journal\": \"Frontiers in cell and developmental biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct genome-wide chromatin occupancy mapping, single lab\",\n      \"pmids\": [\"35493110\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"Partial depletion of BDP7 (PF3D7_1124300) showed no significant effect on parasite viability, indicating BDP7 is not individually essential under standard growth conditions at the depletion levels achieved.\",\n      \"method\": \"Conditional knockdown (DiCre-based), growth assay\",\n      \"journal\": \"Frontiers in cell and developmental biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — negative result from conditional knockdown, single lab, partial depletion may limit interpretation\",\n      \"pmids\": [\"35493110\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2012,\n      \"finding\": \"BDP7 (PF3D7_1124300) was identified as a nuclear protein in P. falciparum by high-accuracy mass spectrometry of an experimentally determined nuclear proteome, with nuclear localization validated by transgenic cell line imaging.\",\n      \"method\": \"Nuclear fractionation followed by mass spectrometry; validation by transgenic GFP-tagged cell lines and fluorescence microscopy\",\n      \"journal\": \"Genome biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — nuclear fractionation plus mass spectrometry plus transgenic fluorescence validation, but this is a proteome-wide study with limited gene-specific mechanistic depth\",\n      \"pmids\": [\"23181666\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"BDP7 (PF3D7_1124300) is a nuclear bromodomain-containing protein in P. falciparum that constitutively associates with PfBDP1 and PfBDP2 as part of a core chromatin regulatory complex; it co-occupies heterochromatic regions across all variant surface antigen (VSA) gene families together with PfBDP1 in early schizonts, and its conditional depletion de-represses VSA expression at both the mRNA and protein levels, establishing BDP7 as a novel player in heterochromatin-mediated silencing of P. falciparum virulence gene families, while also contributing to invasion gene transcription at schizont-stage promoters.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"BDP7 (PF3D7_1124300) is a nuclear bromodomain-containing protein of Plasmodium falciparum that functions in heterochromatin-mediated silencing of clonally variant virulence gene families [#1, #4]. It is a constitutive component of the PfBDP1/BDP2 core chromatin complex, physically associating with PfBDP1, and shares genome-wide chromatin occupancy with PfBDP1 both at invasion gene promoters in mature schizonts and across the gene bodies of variant surface antigen (VSA) families within heterochromatin in early schizonts [#0, #2]. Conditional depletion of BDP7 de-represses the PfEMP1, RIFIN, STEVOR, and PfMC-2TM VSA families at the mRNA and protein levels, establishing a direct role in maintaining their silencing [#1]. Partial depletion does not detectably impair parasite viability under standard culture, indicating BDP7 is not individually essential at the depletion levels achieved [#3]. Beyond its complex membership, chromatin association, and silencing phenotype, no further mechanistic detail—such as the histone modifications recognized by its predicted bromodomain—has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2012,\n      \"claim\": \"Before any functional annotation, establishing where BDP7 resides in the cell was the first step toward its role; this showed BDP7 is a nuclear protein, consistent with a chromatin-associated function.\",\n      \"evidence\": \"Nuclear fractionation and mass spectrometry of the P. falciparum nuclear proteome, validated by transgenic GFP-tagged fluorescence microscopy\",\n      \"pmids\": [\"23181666\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional or mechanistic role assigned in this study\",\n        \"No interaction partners or chromatin targets identified\",\n        \"Proteome-wide survey with limited gene-specific depth\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"The open question of whether BDP7 acts alone or within a complex was resolved by showing it constitutively associates with PfBDP1/BDP2 and co-occupies invasion gene promoters, placing BDP7 in a defined core chromatin regulatory complex.\",\n      \"evidence\": \"Co-immunoprecipitation and ChIP-seq in mature schizont-stage parasites, with bromodomain fold predicted by structural modelling\",\n      \"pmids\": [\"35493110\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Bromodomain ligand/histone mark recognized by BDP7 not experimentally determined\",\n        \"Stoichiometry and architecture of the BDP1/BDP2/BDP7 complex unresolved\",\n        \"Direct DNA/chromatin binding by BDP7 versus recruitment via PfBDP1 not distinguished\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"Whether BDP7 has a causal role in gene regulation was tested by depletion, showing it is required to keep the VSA virulence gene families silenced at both transcript and protein levels.\",\n      \"evidence\": \"DiCre-based conditional knockdown with RT-qPCR and Western blot/flow cytometry readouts of PfEMP1, RIFIN, STEVOR, and PfMC-2TM\",\n      \"pmids\": [\"35493110\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Whether BDP7 silencing acts through the bromodomain or another domain is untested\",\n        \"Direct versus indirect contribution to each VSA family not dissected\",\n        \"Single lab; partial depletion may underestimate effects\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"Mapping where BDP7 acts on the genome tied its silencing role to a physical location, showing co-enrichment with PfBDP1 in heterochromatin across VSA gene bodies in early schizonts.\",\n      \"evidence\": \"ChIP-seq chromatin occupancy mapping\",\n      \"pmids\": [\"35493110\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Histone modification landscape underlying BDP7 recruitment not defined\",\n        \"Temporal dynamics of occupancy across the cell cycle not fully resolved\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"The question of essentiality was addressed, showing partial BDP7 depletion does not detectably affect viability, indicating BDP7 is not individually essential at the depletion achieved.\",\n      \"evidence\": \"DiCre-based conditional knockdown with growth assay\",\n      \"pmids\": [\"35493110\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Negative result limited by incomplete depletion\",\n        \"Possible essentiality under stress or in specific life-cycle stages untested\",\n        \"Redundancy with other complex members not evaluated\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown what chromatin mark BDP7's predicted bromodomain recognizes and whether that recognition is mechanistically required for its role in complex assembly, heterochromatin targeting, and VSA silencing.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No experimental demonstration of a histone-mark ligand for the BDP7 bromodomain\",\n        \"No structure of BDP7 or the intact BDP1/BDP2/BDP7 complex\",\n        \"Separation-of-function mutants distinguishing binding from silencing not reported\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0140030\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [4, 0]},\n      {\"term_id\": \"GO:0000792\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006342\", \"supporting_discovery_ids\": [1, 2]}\n    ],\n    \"complexes\": [\n      \"PfBDP1/BDP2 core chromatin complex (BDP7 as constitutive component) [#0]\"\n    ],\n    \"partners\": [\n      \"PfBDP1 [#0, #2]\",\n      \"PfBDP2 (as part of the core complex) [#0]\"\n    ],\n    \"other_free_text\": [\n      \"Conditional depletion de-represses VSA families PfEMP1, RIFIN, STEVOR, and PfMC-2TM at mRNA and protein levels [#1]\",\n      \"Co-occupies heterochromatin across VSA gene bodies with PfBDP1 in early schizonts [#2]\",\n      \"Occupies invasion gene promoters in mature schizonts [#0]\",\n      \"Not individually essential for viability at partial depletion under standard growth [#3]\",\n      \"Predicted typical bromodomain fold by structural modelling; ligand not experimentally determined [#0]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1124300","name":null,"product":"bromodomain protein 7","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0924000","name":null,"product":"conserved protein, unknown function","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0922100","name":null,"product":"bromodomain protein 7, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"BDP7","evaluation":null}