{"gene":"PF3D7_1123500","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2018,"finding":"GP2 (PF3D7_1123500) is a transmembrane protein that forms a complex with Golgi Protein 1 (GP1) in P. falciparum; this Golgi Protein complex localises to the cis-Golgi throughout the erythrocytic cycle and potentially also during mosquito stages.","method":"Co-immunoprecipitation / complex identification; fluorescence localization studies in parasite strains","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — complex formation and cis-Golgi localization established by direct experiment in a single lab with multiple complementary approaches (co-IP, localization, conditional repression, gene inactivation)","pmids":["29579782"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2018,"finding":"GP2 (PF3D7_1123500) gene inactivation (knockout), alone or combined with conditional repression of GP1, demonstrated that the GP1-GP2 Golgi Protein complex is not essential for parasite viability at any life-cycle stage but is important for optimal asexual blood-stage development.","method":"Gene knockout of GP2 combined with conditional repression of GP1; parasite growth/development phenotyping","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean loss-of-function with defined growth phenotype, single lab, multiple genetic manipulations in one study","pmids":["29579782"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"GP2 (PF3D7_1123500) is a transmembrane Golgi-resident protein of P. falciparum that forms a complex with GP1 at the cis-Golgi throughout the erythrocytic cycle; this complex is dispensable for parasite viability but is required for optimal asexual blood-stage development."},"narrative":{"mechanistic_narrative":"GP2 (PF3D7_1123500) is a transmembrane Golgi-resident protein of Plasmodium falciparum that partners with Golgi Protein 1 (GP1) to form a Golgi Protein complex localizing to the cis-Golgi throughout the erythrocytic cycle, and potentially during mosquito stages [PMID:29579782]. Gene inactivation of GP2, alone or combined with conditional repression of GP1, showed that this complex is dispensable for parasite viability at any life-cycle stage but is required for optimal asexual blood-stage development [PMID:29579782]. Beyond its cis-Golgi localization, complex partnership with GP1, and this growth phenotype, no further molecular function, cargo, or mechanism for GP2 has been characterized in the available corpus.","teleology":[{"year":2018,"claim":"Establishing that PF3D7_1123500 is a bona fide Golgi component required demonstrating both its subcellular residence and a stable binding partner, defining it as a subunit of a discrete Golgi Protein complex rather than an isolated protein.","evidence":"Co-immunoprecipitation and fluorescence localization in P. falciparum strains","pmids":["29579782"],"confidence":"Medium","gaps":["The molecular activity of GP2 within the complex is undefined","No cargo, substrate, or trafficking role assigned to the GP1-GP2 complex","Whether GP2 localization depends on GP1 (or vice versa) was not resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2018,"claim":"Determining whether the Golgi Protein complex is required for the parasite answered its physiological importance: knockout of GP2 combined with GP1 repression showed the complex is non-essential for viability but supports optimal asexual blood-stage growth.","evidence":"Gene knockout of GP2 with conditional repression of GP1 and growth/development phenotyping in P. falciparum","pmids":["29579782"],"confidence":"Medium","gaps":["The mechanistic basis of the suboptimal asexual growth defect is unknown","The specific Golgi function GP2 contributes to was not identified","Life-cycle stages beyond asexual blood stage were not phenotyped in detail"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The molecular function of GP2 within the cis-Golgi and the pathway by which its loss impairs asexual development remain unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No enzymatic or trafficking activity assigned to GP2","No cargo or downstream effectors of the GP1-GP2 complex identified","No structural model of the complex or of GP2's transmembrane topology"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["GP1-GP2 Golgi Protein complex"],"partners":["GP1 (GOLGI PROTEIN 1)"],"other_free_text":["Transmembrane protein [#0]","GP1-GP2 complex is dispensable for viability but required for optimal asexual blood-stage development [#1]","Complex localizes to cis-Golgi throughout the erythrocytic cycle and potentially during mosquito stages [#0]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"29579782","id":"PMC_29579782","title":"Identification of a Golgi apparatus protein complex important for the asexual erythrocytic cycle of the malaria parasite Plasmodium falciparum.","date":"2018","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/29579782","citation_count":13,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["GP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37681888","id":"","title":"Camillo Golgi's Impact on Malaria Studies.","date":"2023","source":"Cells","url":"https://pubmed.ncbi.nlm.nih.gov/37681888","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["GP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"29628927","id":"","title":"Evaluating Human Immune Responses for Vaccine Development in a Novel Human Spleen Cell-Engrafted NOD-SCID-IL2rγNull Mouse Model.","date":"2018","source":"Frontiers in immunology","url":"https://pubmed.ncbi.nlm.nih.gov/29628927","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["GP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"18628827","id":"","title":"Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses.","date":"2008","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/18628827","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["GP2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":6356,"output_tokens":598,"usd":0.014019,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5173,"output_tokens":1418,"usd":0.030658,"stage2_stop_reason":"end_turn"},"total_usd":0.044677,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2018,\n      \"finding\": \"GP2 (PF3D7_1123500) is a transmembrane protein that forms a complex with Golgi Protein 1 (GP1) in P. falciparum; this Golgi Protein complex localises to the cis-Golgi throughout the erythrocytic cycle and potentially also during mosquito stages.\",\n      \"method\": \"Co-immunoprecipitation / complex identification; fluorescence localization studies in parasite strains\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — complex formation and cis-Golgi localization established by direct experiment in a single lab with multiple complementary approaches (co-IP, localization, conditional repression, gene inactivation)\",\n      \"pmids\": [\"29579782\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"GP2 (PF3D7_1123500) gene inactivation (knockout), alone or combined with conditional repression of GP1, demonstrated that the GP1-GP2 Golgi Protein complex is not essential for parasite viability at any life-cycle stage but is important for optimal asexual blood-stage development.\",\n      \"method\": \"Gene knockout of GP2 combined with conditional repression of GP1; parasite growth/development phenotyping\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean loss-of-function with defined growth phenotype, single lab, multiple genetic manipulations in one study\",\n      \"pmids\": [\"29579782\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"GP2 (PF3D7_1123500) is a transmembrane Golgi-resident protein of P. falciparum that forms a complex with GP1 at the cis-Golgi throughout the erythrocytic cycle; this complex is dispensable for parasite viability but is required for optimal asexual blood-stage development.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"GP2 (PF3D7_1123500) is a transmembrane Golgi-resident protein of Plasmodium falciparum that partners with Golgi Protein 1 (GP1) to form a Golgi Protein complex localizing to the cis-Golgi throughout the erythrocytic cycle, and potentially during mosquito stages [#0]. Gene inactivation of GP2, alone or combined with conditional repression of GP1, showed that this complex is dispensable for parasite viability at any life-cycle stage but is required for optimal asexual blood-stage development [#1]. Beyond its cis-Golgi localization, complex partnership with GP1, and this growth phenotype, no further molecular function, cargo, or mechanism for GP2 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2018,\n      \"claim\": \"Establishing that PF3D7_1123500 is a bona fide Golgi component required demonstrating both its subcellular residence and a stable binding partner, defining it as a subunit of a discrete Golgi Protein complex rather than an isolated protein.\",\n      \"evidence\": \"Co-immunoprecipitation and fluorescence localization in P. falciparum strains\",\n      \"pmids\": [\n        \"29579782\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The molecular activity of GP2 within the complex is undefined\",\n        \"No cargo, substrate, or trafficking role assigned to the GP1-GP2 complex\",\n        \"Whether GP2 localization depends on GP1 (or vice versa) was not resolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Determining whether the Golgi Protein complex is required for the parasite answered its physiological importance: knockout of GP2 combined with GP1 repression showed the complex is non-essential for viability but supports optimal asexual blood-stage growth.\",\n      \"evidence\": \"Gene knockout of GP2 with conditional repression of GP1 and growth/development phenotyping in P. falciparum\",\n      \"pmids\": [\n        \"29579782\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The mechanistic basis of the suboptimal asexual growth defect is unknown\",\n        \"The specific Golgi function GP2 contributes to was not identified\",\n        \"Life-cycle stages beyond asexual blood stage were not phenotyped in detail\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function of GP2 within the cis-Golgi and the pathway by which its loss impairs asexual development remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No enzymatic or trafficking activity assigned to GP2\",\n        \"No cargo or downstream effectors of the GP1-GP2 complex identified\",\n        \"No structural model of the complex or of GP2's transmembrane topology\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0005801\",\n        \"supporting_discovery_ids\": [0]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"GP1-GP2 Golgi Protein complex\"\n    ],\n    \"partners\": [\n      \"GP1 (Golgi Protein 1)\"\n    ],\n    \"other_free_text\": [\n      \"Transmembrane protein [#0]\",\n      \"GP1-GP2 complex is dispensable for viability but required for optimal asexual blood-stage development [#1]\",\n      \"Complex localizes to cis-Golgi throughout the erythrocytic cycle and potentially during mosquito stages [#0]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1123500","name":null,"product":"golgi protein 2","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0924800","name":null,"product":"golgi protein 2","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0921300","name":null,"product":"golgi protein 2, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"GP2","evaluation":null}