{"gene":"PF3D7_1107700","run_date":"2026-09-08T12:51:28","timeline":{"discoveries":[{"year":2004,"finding":"PES (PfPES, PF3D7_1107700), the Plasmodium falciparum ortholog of the nuclear pescadillo protein, was characterized and shown to interact with PfYVH1, a dual-specificity zinc-finger phosphatase, implicating PfYVH1 in the regulation of parasitic development.","method":"Biochemical characterization of recombinant proteins; protein–protein interaction assay (pulldown/co-precipitation) between PfPES and PfYVH1","journal":"Molecular and biochemical parasitology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single lab, single interaction assay method described in abstract without detailed reciprocal validation; no functional consequence of the interaction experimentally resolved beyond inference","pmids":["14698441"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PES (PF3D7_1107700), the P. falciparum pescadillo ortholog, is a nuclear protein that physically interacts with the dual-specificity phosphatase PfYVH1, suggesting a role in nuclear regulation of parasite development, but no further mechanistic details about PES itself have been established."},"narrative":{"mechanistic_narrative":"PES (PF3D7_1107700), the Plasmodium falciparum ortholog of the nuclear pescadillo protein, was characterized as a physical interaction partner of PfYVH1, a dual-specificity zinc-finger phosphatase, an association taken to implicate PfYVH1 in the regulation of parasitic development [PMID:14698441]. Beyond this single interaction, no further mechanistic detail about PES itself — its molecular activity, its own developmental function, or the consequence of the PfYVH1 interaction — has been characterized in the available corpus.","teleology":[{"year":2004,"claim":"To place the P. falciparum pescadillo ortholog in a molecular context, its physical partners were sought, establishing a direct interaction with the dual-specificity phosphatase PfYVH1.","evidence":"Biochemical characterization of recombinant proteins with a protein–protein interaction (pulldown/co-precipitation) assay between PfPES and PfYVH1","pmids":["14698441"],"confidence":"Low","gaps":["Single interaction assay described without reciprocal validation; interaction not independently confirmed","No functional consequence of the PES–PfYVH1 interaction experimentally resolved","Intrinsic molecular activity and developmental role of PES itself undefined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"What PES does mechanistically — its own biochemical activity, subcellular localization in the parasite, and the developmental output of its interaction with PfYVH1 — remains unresolved.","evidence":"","pmids":[],"confidence":"Low","gaps":["No functional or knockout phenotype for PES reported","No structural model or defined enzymatic/regulatory activity","Recruitment and pathway context of the PES–PfYVH1 complex unknown"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["PFYVH1 (DUAL-SPECIFICITY ZINC-FINGER PHOSPHATASE)"],"other_free_text":["PES (PF3D7_1107700) is the P. falciparum ortholog of the nuclear pescadillo protein and physically interacts with PfYVH1 [#0].","The corpus establishes nothing about PES beyond this single interaction; the developmental-regulation inference in the source concerns PfYVH1, not a directly demonstrated PES function."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"14698441","id":"PMC_14698441","title":"A zinc-binding dual-specificity YVH1 phosphatase in the malaria parasite, Plasmodium falciparum, and its interaction with the nuclear protein, pescadillo.","date":"2004","source":"Molecular and biochemical parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/14698441","citation_count":27,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3407,"output_tokens":371,"usd":0.007893,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4852,"output_tokens":982,"usd":0.024405,"stage2_stop_reason":"end_turn"},"total_usd":0.032298,"stage1_batch_id":"msgbatch_01DisAaTypwmC8QwdMBuePHo","stage2_batch_id":"msgbatch_0184zxZTqxiEfdYYfPMfkqjb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2004,\n      \"finding\": \"PES (PfPES, PF3D7_1107700), the Plasmodium falciparum ortholog of the nuclear pescadillo protein, was characterized and shown to interact with PfYVH1, a dual-specificity zinc-finger phosphatase, implicating PfYVH1 in the regulation of parasitic development.\",\n      \"method\": \"Biochemical characterization of recombinant proteins; protein–protein interaction assay (pulldown/co-precipitation) between PfPES and PfYVH1\",\n      \"journal\": \"Molecular and biochemical parasitology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab, single interaction assay method described in abstract without detailed reciprocal validation; no functional consequence of the interaction experimentally resolved beyond inference\",\n      \"pmids\": [\"14698441\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PES (PF3D7_1107700), the P. falciparum pescadillo ortholog, is a nuclear protein that physically interacts with the dual-specificity phosphatase PfYVH1, suggesting a role in nuclear regulation of parasite development, but no further mechanistic details about PES itself have been established.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PES (PF3D7_1107700), the Plasmodium falciparum ortholog of the nuclear pescadillo protein, was characterized as a physical interaction partner of PfYVH1, a dual-specificity zinc-finger phosphatase, an association taken to implicate PfYVH1 in the regulation of parasitic development [#0]. Beyond this single interaction, no further mechanistic detail about PES itself — its molecular activity, its own developmental function, or the consequence of the PfYVH1 interaction — has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2004,\n      \"claim\": \"To place the P. falciparum pescadillo ortholog in a molecular context, its physical partners were sought, establishing a direct interaction with the dual-specificity phosphatase PfYVH1.\",\n      \"evidence\": \"Biochemical characterization of recombinant proteins with a protein–protein interaction (pulldown/co-precipitation) assay between PfPES and PfYVH1\",\n      \"pmids\": [\"14698441\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single interaction assay described without reciprocal validation; interaction not independently confirmed\",\n        \"No functional consequence of the PES–PfYVH1 interaction experimentally resolved\",\n        \"Intrinsic molecular activity and developmental role of PES itself undefined\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"What PES does mechanistically — its own biochemical activity, subcellular localization in the parasite, and the developmental output of its interaction with PfYVH1 — remains unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No functional or knockout phenotype for PES reported\",\n        \"No structural model or defined enzymatic/regulatory activity\",\n        \"Recruitment and pathway context of the PES–PfYVH1 complex unknown\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\"PfYVH1 (dual-specificity zinc-finger phosphatase)\"],\n    \"other_free_text\": [\n      \"PES (PF3D7_1107700) is the P. falciparum ortholog of the nuclear pescadillo protein and physically interacts with PfYVH1 [#0].\",\n      \"The corpus establishes nothing about PES beyond this single interaction; the developmental-regulation inference in the source concerns PfYVH1, not a directly demonstrated PES function.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1107700","name":null,"product":"pescadillo homolog","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0939200","name":null,"product":"pescadillo homolog, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0905300","name":null,"product":"pescadillo homolog, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"PES","evaluation":null}