{"gene":"PF3D7_1017100","run_date":"2026-09-08T12:51:28","timeline":{"discoveries":[{"year":2013,"finding":"RON12 (PF3D7_1017100) is a Plasmodium-specific rhoptry neck protein that lacks a membrane anchor and is a major soluble component of the nascent parasitophorous vacuole (PV). The bulk of RON12 secretion occurs late during invasion (after parasite internalization), allowing accumulation in the fully formed PV, with a small proportion occasionally apparent in structures resembling the moving junction. Deletion of the ron12 gene is not lethal but does affect parasite proliferation.","method":"Gene deletion (knockout), immunofluorescence assay, immunoelectron microscopy, fractionation/localization experiments in P. falciparum merozoites","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean KO with defined proliferation phenotype plus direct subcellular localization by IFA and IEM, single lab","pmids":["23937520"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2018,"finding":"RON12 (PF3D7_1017100) localizes to the rhoptry neck in P. falciparum and rodent malaria merozoites, but in P. berghei sporozoites it localizes to the rhoptry body rather than the rhoptry neck, indicating a stage-specific difference in organelle localization. Gene disruption of ron12 in P. berghei demonstrated that RON12 is dispensable for sporogony, invasion of mosquito salivary glands and mouse hepatocytes, and development in hepatocytes.","method":"Immunofluorescence assay, gene disruption (knockout) in P. berghei, phenotypic analysis of sporogony and hepatocyte invasion","journal":"Parasitology international","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct localization by IFA in two life-cycle stages plus clean KO with defined negative phenotypic readout, single lab","pmids":["30290224"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2018,"finding":"RON12 (PF3D7_1017100) localizes to the rhoptry neck of merozoites within schizonts and to the surface of free merozoites. Under non-reducing conditions, native PfRON12 forms a disulfide-bond-mediated multimer (~80 kDa), whereas the monomer migrates at ~40 kDa under reducing conditions. Rabbit antibodies against recombinant PfRON12 significantly inhibit merozoite invasion of erythrocytes in vitro.","method":"Immunoblot under reducing and non-reducing conditions, immunofluorescence assay, immunoelectron microscopy, in vitro growth inhibition assay (GIA) with anti-PfRON12 antibodies","journal":"Parasitology international","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — multiple orthogonal methods (IEM, IFA, GIA, non-reducing SDS-PAGE) in single lab establishing localization, oligomeric state, and functional role in invasion","pmids":["30342119"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"finding":"RON12 (PF3D7_1017100 ortholog in P. berghei) has the highest transcript levels among rhoptry molecules in sporozoites and was used as a bait (tagged with UltraID) in a proximity-dependent biotinylation screen. In RON12::UltraID transgenic sporozoites, known rhoptry proteins and a novel rhoptry molecule (PBANKA_1363400) were biotinylated during parasite maturation in mosquitoes, placing RON12 in proximity to these secretory proteins in the sporozoite rhoptry compartment.","method":"Proximity-dependent biotin identification (UltraID), genome editing/tagging strategy, mass spectrometry, transcriptomics in P. berghei sporozoites","journal":"Frontiers in cellular and infection microbiology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — proximity labeling identifies co-localized proteins but does not establish direct interaction; single lab, single method for the RON12-specific findings","pmids":["41668734"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"RON12 (PF3D7_1017100) is a Plasmodium-specific, membrane-anchor-lacking rhoptry neck protein that is secreted predominantly late during erythrocyte invasion to accumulate as a soluble component of the parasitophorous vacuole; it forms disulfide-bond-mediated multimers, contributes to (but is not essential for) merozoite proliferation, localizes to the rhoptry body rather than the neck in sporozoites (where it is also dispensable for host cell invasion), and antibodies against it inhibit erythrocyte invasion in vitro."},"narrative":{"mechanistic_narrative":"RON12 (PF3D7_1017100) is a Plasmodium-specific rhoptry protein that contributes to host cell invasion and is secreted into the nascent parasitophorous vacuole during erythrocyte invasion [PMID:23937520]. Unlike membrane-anchored rhoptry components, RON12 lacks a membrane anchor and accumulates as a major soluble component of the fully formed parasitophorous vacuole, with the bulk of its secretion occurring late in invasion after merozoite internalization and only a minor fraction associated with structures resembling the moving junction [PMID:23937520]. In P. falciparum merozoites it localizes to the rhoptry neck within schizonts and to the surface of free merozoites, and native protein forms a disulfide-bond-mediated ~80 kDa multimer that resolves to a ~40 kDa monomer under reducing conditions [PMID:30342119]. Antibodies raised against recombinant PfRON12 significantly inhibit merozoite invasion of erythrocytes in vitro, implicating RON12 in the invasion process [PMID:30342119], though genetic deletion is not lethal and instead affects parasite proliferation [PMID:23937520]. RON12 function is stage- and species-divergent: in P. berghei sporozoites it localizes to the rhoptry body rather than the neck and is dispensable for sporogony, salivary gland invasion, hepatocyte invasion, and liver-stage development [PMID:30290224]. No molecular partners or direct binding activity of RON12 have been established in the available corpus beyond its proximity to other rhoptry proteins in sporozoites [PMID:41668734].","teleology":[{"year":2013,"claim":"Establishing that RON12 is a soluble, non-membrane-anchored rhoptry neck protein secreted late into the parasitophorous vacuole distinguished it from anchored moving-junction components and defined when and where it acts during invasion.","evidence":"Gene knockout, IFA, immunoelectron microscopy, and fractionation in P. falciparum merozoites","pmids":["23937520"],"confidence":"Medium","gaps":["The molecular function of RON12 within the parasitophorous vacuole is undefined","The mechanistic basis of the proliferation defect upon deletion is unknown","No binding partners or substrates identified"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2018,"claim":"Comparing localization and knockout phenotypes across life-cycle stages and species showed RON12 relocates from rhoptry neck (blood-stage merozoites) to rhoptry body (sporozoites) and is dispensable for the pre-erythrocytic stages, revealing stage-specific deployment.","evidence":"IFA in two life-cycle stages plus gene disruption and phenotypic analysis of sporogony and hepatocyte invasion in P. berghei","pmids":["30290224"],"confidence":"Medium","gaps":["Why RON12 localization differs between blood and sporozoite stages is unexplained","Dispensability in sporozoites contrasts with the invasion role in erythrocytes but the reason is not resolved","No functional readout linking sporozoite rhoptry-body localization to any process"],"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2018,"claim":"Demonstrating that native PfRON12 forms disulfide-linked multimers and that anti-PfRON12 antibodies block erythrocyte invasion provided the first direct functional evidence tying RON12 to the invasion process and defined its oligomeric state.","evidence":"Reducing/non-reducing immunoblot, IFA, immunoelectron microscopy, and in vitro growth inhibition assay with anti-PfRON12 antibodies in P. falciparum","pmids":["30342119"],"confidence":"Medium","gaps":["The molecular target/receptor engaged by RON12 during invasion is unidentified","How antibody binding mechanistically blocks invasion is unknown","The functional significance of multimerization is not tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2026,"claim":"Using RON12 as a proximity-labeling bait in sporozoites placed it near known and novel rhoptry proteins, generating candidate neighbors but not direct interactors.","evidence":"UltraID proximity-dependent biotinylation, mass spectrometry, and transcriptomics in P. berghei sporozoites","pmids":["41668734"],"confidence":"Low","gaps":["Proximity labeling identifies co-localized proteins but does not establish direct interaction","The novel rhoptry molecule PBANKA_1363400 is not functionally linked to RON12","Findings are in P. berghei sporozoites and single-method for RON12"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The molecular function of RON12 — its binding partners, any receptor engagement, and the mechanism by which it contributes to invasion and proliferation — remains undefined.","evidence":"","pmids":[],"confidence":"Low","gaps":["No direct interacting partner or substrate established","No structural model or defined biochemical activity","Mechanistic basis of the proliferation phenotype unresolved"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":[],"other_free_text":["Plasmodium-specific rhoptry protein lacking a membrane anchor; major soluble component of the nascent parasitophorous vacuole [#0]","Forms disulfide-bond-mediated multimer (~80 kDa) resolving to ~40 kDa monomer under reducing conditions [#2]","Anti-PfRON12 antibodies inhibit merozoite invasion of erythrocytes in vitro [#2]","Gene deletion is non-lethal but affects merozoite proliferation [#0]","Stage- and species-divergent localization: rhoptry neck in merozoites, rhoptry body in P. berghei sporozoites where it is dispensable for invasion [#1]","In P. berghei sporozoites, proximity labeling places RON12 near known rhoptry proteins and a novel rhoptry molecule PBANKA_1363400 [#3]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"23937520","id":"PMC_23937520","title":"RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation.","date":"2013","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/23937520","citation_count":21,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RON12"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"30290224","id":"PMC_30290224","title":"Plasmodium RON12 localizes to the rhoptry body in sporozoites.","date":"2018","source":"Parasitology international","url":"https://pubmed.ncbi.nlm.nih.gov/30290224","citation_count":7,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RON12","PfRON12"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"30342119","id":"PMC_30342119","title":"Antibodies against a Plasmodium falciparum RON12 inhibit merozoite invasion into erythrocytes.","date":"2018","source":"Parasitology international","url":"https://pubmed.ncbi.nlm.nih.gov/30342119","citation_count":4,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RON12","PfRON12"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41668734","id":"PMC_41668734","title":"Identification of a novel rhoptry protein expressed predominantly in Plasmodium sporozoites.","date":"2026","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/41668734","citation_count":1,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RON12"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":4979,"output_tokens":1444,"usd":0.018298,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6385,"output_tokens":2567,"usd":0.04805,"stage2_stop_reason":"end_turn"},"total_usd":0.066348,"stage1_batch_id":"msgbatch_01DisAaTypwmC8QwdMBuePHo","stage2_batch_id":"msgbatch_0184zxZTqxiEfdYYfPMfkqjb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2013,\n      \"finding\": \"RON12 (PF3D7_1017100) is a Plasmodium-specific rhoptry neck protein that lacks a membrane anchor and is a major soluble component of the nascent parasitophorous vacuole (PV). The bulk of RON12 secretion occurs late during invasion (after parasite internalization), allowing accumulation in the fully formed PV, with a small proportion occasionally apparent in structures resembling the moving junction. Deletion of the ron12 gene is not lethal but does affect parasite proliferation.\",\n      \"method\": \"Gene deletion (knockout), immunofluorescence assay, immunoelectron microscopy, fractionation/localization experiments in P. falciparum merozoites\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean KO with defined proliferation phenotype plus direct subcellular localization by IFA and IEM, single lab\",\n      \"pmids\": [\"23937520\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"RON12 (PF3D7_1017100) localizes to the rhoptry neck in P. falciparum and rodent malaria merozoites, but in P. berghei sporozoites it localizes to the rhoptry body rather than the rhoptry neck, indicating a stage-specific difference in organelle localization. Gene disruption of ron12 in P. berghei demonstrated that RON12 is dispensable for sporogony, invasion of mosquito salivary glands and mouse hepatocytes, and development in hepatocytes.\",\n      \"method\": \"Immunofluorescence assay, gene disruption (knockout) in P. berghei, phenotypic analysis of sporogony and hepatocyte invasion\",\n      \"journal\": \"Parasitology international\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization by IFA in two life-cycle stages plus clean KO with defined negative phenotypic readout, single lab\",\n      \"pmids\": [\"30290224\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"RON12 (PF3D7_1017100) localizes to the rhoptry neck of merozoites within schizonts and to the surface of free merozoites. Under non-reducing conditions, native PfRON12 forms a disulfide-bond-mediated multimer (~80 kDa), whereas the monomer migrates at ~40 kDa under reducing conditions. Rabbit antibodies against recombinant PfRON12 significantly inhibit merozoite invasion of erythrocytes in vitro.\",\n      \"method\": \"Immunoblot under reducing and non-reducing conditions, immunofluorescence assay, immunoelectron microscopy, in vitro growth inhibition assay (GIA) with anti-PfRON12 antibodies\",\n      \"journal\": \"Parasitology international\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — multiple orthogonal methods (IEM, IFA, GIA, non-reducing SDS-PAGE) in single lab establishing localization, oligomeric state, and functional role in invasion\",\n      \"pmids\": [\"30342119\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"RON12 (PF3D7_1017100 ortholog in P. berghei) has the highest transcript levels among rhoptry molecules in sporozoites and was used as a bait (tagged with UltraID) in a proximity-dependent biotinylation screen. In RON12::UltraID transgenic sporozoites, known rhoptry proteins and a novel rhoptry molecule (PBANKA_1363400) were biotinylated during parasite maturation in mosquitoes, placing RON12 in proximity to these secretory proteins in the sporozoite rhoptry compartment.\",\n      \"method\": \"Proximity-dependent biotin identification (UltraID), genome editing/tagging strategy, mass spectrometry, transcriptomics in P. berghei sporozoites\",\n      \"journal\": \"Frontiers in cellular and infection microbiology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — proximity labeling identifies co-localized proteins but does not establish direct interaction; single lab, single method for the RON12-specific findings\",\n      \"pmids\": [\"41668734\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"RON12 (PF3D7_1017100) is a Plasmodium-specific, membrane-anchor-lacking rhoptry neck protein that is secreted predominantly late during erythrocyte invasion to accumulate as a soluble component of the parasitophorous vacuole; it forms disulfide-bond-mediated multimers, contributes to (but is not essential for) merozoite proliferation, localizes to the rhoptry body rather than the neck in sporozoites (where it is also dispensable for host cell invasion), and antibodies against it inhibit erythrocyte invasion in vitro.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"RON12 (PF3D7_1017100) is a Plasmodium-specific rhoptry protein that contributes to host cell invasion and is secreted into the nascent parasitophorous vacuole during erythrocyte invasion [#0]. Unlike membrane-anchored rhoptry components, RON12 lacks a membrane anchor and accumulates as a major soluble component of the fully formed parasitophorous vacuole, with the bulk of its secretion occurring late in invasion after merozoite internalization and only a minor fraction associated with structures resembling the moving junction [#0]. In P. falciparum merozoites it localizes to the rhoptry neck within schizonts and to the surface of free merozoites, and native protein forms a disulfide-bond-mediated ~80 kDa multimer that resolves to a ~40 kDa monomer under reducing conditions [#2]. Antibodies raised against recombinant PfRON12 significantly inhibit merozoite invasion of erythrocytes in vitro, implicating RON12 in the invasion process [#2], though genetic deletion is not lethal and instead affects parasite proliferation [#0]. RON12 function is stage- and species-divergent: in P. berghei sporozoites it localizes to the rhoptry body rather than the neck and is dispensable for sporogony, salivary gland invasion, hepatocyte invasion, and liver-stage development [#1]. No molecular partners or direct binding activity of RON12 have been established in the available corpus beyond its proximity to other rhoptry proteins in sporozoites [#3].\",\n  \"teleology\": [\n    {\n      \"year\": 2013,\n      \"claim\": \"Establishing that RON12 is a soluble, non-membrane-anchored rhoptry neck protein secreted late into the parasitophorous vacuole distinguished it from anchored moving-junction components and defined when and where it acts during invasion.\",\n      \"evidence\": \"Gene knockout, IFA, immunoelectron microscopy, and fractionation in P. falciparum merozoites\",\n      \"pmids\": [\"23937520\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The molecular function of RON12 within the parasitophorous vacuole is undefined\",\n        \"The mechanistic basis of the proliferation defect upon deletion is unknown\",\n        \"No binding partners or substrates identified\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Comparing localization and knockout phenotypes across life-cycle stages and species showed RON12 relocates from rhoptry neck (blood-stage merozoites) to rhoptry body (sporozoites) and is dispensable for the pre-erythrocytic stages, revealing stage-specific deployment.\",\n      \"evidence\": \"IFA in two life-cycle stages plus gene disruption and phenotypic analysis of sporogony and hepatocyte invasion in P. berghei\",\n      \"pmids\": [\"30290224\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Why RON12 localization differs between blood and sporozoite stages is unexplained\",\n        \"Dispensability in sporozoites contrasts with the invasion role in erythrocytes but the reason is not resolved\",\n        \"No functional readout linking sporozoite rhoptry-body localization to any process\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Demonstrating that native PfRON12 forms disulfide-linked multimers and that anti-PfRON12 antibodies block erythrocyte invasion provided the first direct functional evidence tying RON12 to the invasion process and defined its oligomeric state.\",\n      \"evidence\": \"Reducing/non-reducing immunoblot, IFA, immunoelectron microscopy, and in vitro growth inhibition assay with anti-PfRON12 antibodies in P. falciparum\",\n      \"pmids\": [\"30342119\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The molecular target/receptor engaged by RON12 during invasion is unidentified\",\n        \"How antibody binding mechanistically blocks invasion is unknown\",\n        \"The functional significance of multimerization is not tested\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Using RON12 as a proximity-labeling bait in sporozoites placed it near known and novel rhoptry proteins, generating candidate neighbors but not direct interactors.\",\n      \"evidence\": \"UltraID proximity-dependent biotinylation, mass spectrometry, and transcriptomics in P. berghei sporozoites\",\n      \"pmids\": [\"41668734\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Proximity labeling identifies co-localized proteins but does not establish direct interaction\",\n        \"The novel rhoptry molecule PBANKA_1363400 is not functionally linked to RON12\",\n        \"Findings are in P. berghei sporozoites and single-method for RON12\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function of RON12 — its binding partners, any receptor engagement, and the mechanism by which it contributes to invasion and proliferation — remains undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No direct interacting partner or substrate established\",\n        \"No structural model or defined biochemical activity\",\n        \"Mechanistic basis of the proliferation phenotype unresolved\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0033227\", \"supporting_discovery_ids\": [0, 1, 2]},\n      {\"term_id\": \"GO:0020003\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0009986\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0044409\", \"supporting_discovery_ids\": [0, 2]}\n    ],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Plasmodium-specific rhoptry protein lacking a membrane anchor; major soluble component of the nascent parasitophorous vacuole [#0]\",\n      \"Forms disulfide-bond-mediated multimer (~80 kDa) resolving to ~40 kDa monomer under reducing conditions [#2]\",\n      \"Anti-PfRON12 antibodies inhibit merozoite invasion of erythrocytes in vitro [#2]\",\n      \"Gene deletion is non-lethal but affects merozoite proliferation [#0]\",\n      \"Stage- and species-divergent localization: rhoptry neck in merozoites, rhoptry body in P. berghei sporozoites where it is dispensable for invasion [#1]\",\n      \"In P. berghei sporozoites, proximity labeling places RON12 near known rhoptry proteins and a novel rhoptry molecule PBANKA_1363400 [#3]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1017100","name":null,"product":"rhoptry neck protein 12","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0501400","name":null,"product":"rhoptry neck protein 12","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0601300","name":null,"product":"rhoptry neck protein 12, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"RON12","evaluation":null}