{"gene":"PF3D7_1005500","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2023,"finding":"PfUPF1 (PF3D7_1005500) localizes to puncta within the P. falciparum parasite cytoplasm, as determined by epitope-tagging via CRISPR-Cas9 and fluorescence microscopy.","method":"CRISPR-Cas9 epitope-tagging and fluorescence localization","journal":"mSphere","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — direct localization experiment with epitope tag, single lab, single method","pmids":["37366629"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"finding":"PfUPF1 (PF3D7_1005500) physically interacts with PfUPF2 and other mRNA-binding proteins in P. falciparum, as shown by co-immunoprecipitation of epitope-tagged proteins.","method":"Co-immunoprecipitation of epitope-tagged PfUPF1 and PfUPF2","journal":"mSphere","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — reciprocal co-IP between UPF1 and UPF2 in a single lab study with two tagged proteins","pmids":["37366629"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"finding":"CRISPR-Cas9 disruption of PfUPF1 (PF3D7_1005500) does not affect degradation of nonsense transcripts and does not impair parasite growth ex vivo, establishing that PfUPF1 is non-essential for NMD and for asexual blood-stage proliferation in P. falciparum.","method":"CRISPR-Cas9 knockout followed by RNA-seq and growth assays","journal":"mSphere","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean CRISPR KO with defined transcriptomic readout and growth phenotype, single lab, two orthogonal methods (RNA-seq + growth assay)","pmids":["37366629"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"finding":"In P. yoelii zygotes (post-fertilization), UPF1 becomes associated with ALBA4 proximity proteomics signal, suggesting UPF1 enters or associates with the DOZI/CITH/ALBA regulatory mRNP complex in a stage-dependent manner in zygotes but not female gametocytes.","method":"TurboID-based proximity proteomics (BioID) of ALBA4 in P. yoelii female gametocytes and zygotes","journal":"PLoS pathogens","confidence":"Low","confidence_rationale":"Tier 3 / Weak — proximity labeling (not direct Co-IP), single lab, UPF1 detected as proximal to ALBA4 only in zygotes","pmids":["39777415"],"is_preprint":false,"attribution":[{"species":"Plasmodium yoelii","strain":""}]},{"year":2010,"finding":"In P. berghei female gametocytes, UPF1 homologs are encoded in the genome but are absent from affinity-purified DOZI/CITH mRNP complexes, indicating that UPF1 is NOT a component of the translational repression mRNP in gametocytes.","method":"Affinity purification coupled with mass-spectrometric analysis (negative result)","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — rigorous mass spectrometry-based interactome with explicit negative result, single lab","pmids":["20169188"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"In P. falciparum, UPF1 (PF3D7_1005500) is expressed and localizes to cytoplasmic puncta where it interacts with PfUPF2 and other mRNA-binding proteins, including PfAlba4, but CRISPR-Cas9 knockout demonstrates it is non-essential for nonsense-mediated mRNA decay and for asexual blood-stage parasite growth; in P. yoelii zygotes (but not gametocytes), UPF1 associates with the ALBA4/DOZI/CITH translational regulatory complex in a stage-dependent manner, and in P. berghei gametocytes it is explicitly absent from this complex."},"narrative":{"mechanistic_narrative":"UPF1 (PF3D7_1005500) is a cytoplasmic mRNA-associated protein of Plasmodium that, despite its annotation as the core NMD helicase, is dispensable for nonsense-mediated mRNA decay in P. falciparum blood-stage parasites [PMID:37366629]. Epitope-tagging places PfUPF1 in cytoplasmic puncta, and reciprocal co-immunoprecipitation shows it physically associates with PfUPF2 and additional mRNA-binding proteins [PMID:37366629]. CRISPR-Cas9 disruption neither stabilizes nonsense transcripts nor impairs asexual proliferation, establishing that PfUPF1 is non-essential for both NMD and blood-stage growth [PMID:37366629]. Its association with translational-regulatory mRNP complexes is stage- and species-dependent: in P. berghei female gametocytes UPF1 is explicitly excluded from affinity-purified DOZI/CITH mRNPs [PMID:20169188], whereas in P. yoelii it becomes proximal to ALBA4 specifically in zygotes but not gametocytes [PMID:39777415]. Beyond these findings, the enzymatic activity, RNA substrates, and mechanistic role of PF3D7_1005500 have not been characterized in the available corpus.","teleology":[{"year":2010,"claim":"To test whether UPF1 participates in the gametocyte translational-repression machinery, its presence in the DOZI/CITH mRNP was assayed directly.","evidence":"affinity purification with mass spectrometry of DOZI/CITH complexes in P. berghei female gametocytes (negative result)","pmids":["20169188"],"confidence":"Medium","gaps":["Absence from the purified complex does not exclude transient or low-affinity association","Does not address UPF1 function at other life-cycle stages","No direct characterization of PF3D7_1005500 activity"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2023,"claim":"To define where PfUPF1 acts and with whom, its subcellular distribution and protein partners were determined in P. falciparum.","evidence":"CRISPR-Cas9 epitope-tagging with fluorescence microscopy and reciprocal co-immunoprecipitation of PfUPF1 and PfUPF2","pmids":["37366629"],"confidence":"Medium","gaps":["Identity of the 'other mRNA-binding proteins' co-purifying with PfUPF1 not fully resolved","Whether the UPF1–UPF2 interaction is direct or bridged by RNA not established","Functional consequence of puncta localization unknown"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2023,"claim":"To test the assumed NMD function, PfUPF1 was disrupted and both transcript degradation and growth were measured, revealing it is dispensable for canonical NMD.","evidence":"CRISPR-Cas9 knockout with RNA-seq and ex vivo growth assays in P. falciparum","pmids":["37366629"],"confidence":"Medium","gaps":["Does not identify the actual mRNA quality-control pathway operating in the parasite","Redundancy with a UPF1 paralog not ruled out","No stage-specific phenotype beyond asexual blood stages tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"claim":"To determine whether UPF1 joins translational-regulatory mRNPs during transmission stages, its proximity to ALBA4 was mapped across the female-gametocyte-to-zygote transition.","evidence":"TurboID proximity proteomics of ALBA4 in P. yoelii female gametocytes and zygotes","pmids":["39777415"],"confidence":"Low","gaps":["Proximity labeling infers spatial vicinity, not direct interaction, and lacks reciprocal Co-IP validation","Functional role of UPF1 in zygotes not tested","Single lab, single method"],"attribution":[{"species":"Plasmodium yoelii","strain":""}]},{"year":null,"claim":"The catalytic activity, RNA substrates, and true cellular function of PF3D7_1005500 remain undefined, given that it is dispensable for canonical NMD.","evidence":"","pmids":[],"confidence":"Low","gaps":["No enzymatic or helicase activity demonstrated for PF3D7_1005500","No mRNA targets identified","Mechanistic basis for stage-dependent mRNP association unresolved"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["PfUPF1–PfUPF2 mRNA-binding protein complex (P. falciparum) [#1]","ALBA4/DOZI/CITH regulatory mRNP — UPF1 proximal in P. yoelii zygotes [#3]; UPF1 excluded from DOZI/CITH mRNP in P. berghei gametocytes [#4]"],"partners":["PFUPF2 [#1]","UNIDENTIFIED MRNA-BINDING PROTEINS [#1]","ALBA4 (PROXIMITY, P. YOELII ZYGOTES) [#3]"],"other_free_text":["CRISPR-Cas9 disruption of PF3D7_1005500 does not stabilize nonsense transcripts and does not impair asexual blood-stage growth, so it is non-essential for NMD and proliferation [#2].","Association with translational-regulatory mRNPs is stage- and species-dependent: excluded in P. berghei gametocytes [#4], proximal to ALBA4 in P. yoelii zygotes [#3]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"37366629","id":"PMC_37366629","title":"Nonsense-mediated decay machinery in Plasmodium falciparum is inefficient and non-essential.","date":"2023","source":"mSphere","url":"https://pubmed.ncbi.nlm.nih.gov/37366629","citation_count":10,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PfUPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41277688","id":"","title":"Plasmodium falciparum DNA repair dynamics reveal unique roles for TLS polymerases and PfRad51 in genome diversification.","date":"2025","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/41277688","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39868986","id":"","title":"Ectopic overexpression of Plasmodium falciparum DNA-/RNA-binding Alba proteins misregulates virulence gene homeostasis during asexual blood development.","date":"2025","source":"Microbiology spectrum","url":"https://pubmed.ncbi.nlm.nih.gov/39868986","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39777415","id":"","title":"Widespread release of translational repression across Plasmodium's host-to-vector transmission event.","date":"2025","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/39777415","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36093191","id":"","title":"Single-cell RNA profiling of Plasmodium vivax-infected hepatocytes reveals parasite- and host- specific transcriptomic signatures and therapeutic targets.","date":"2022","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/36093191","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"27230797","id":"","title":"Translational regulation in blood stages of the malaria parasite Plasmodium spp.: systems-wide studies pave the way.","date":"2016","source":"Wiley interdisciplinary reviews. RNA","url":"https://pubmed.ncbi.nlm.nih.gov/27230797","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"21245033","id":"","title":"RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts.","date":"2011","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/21245033","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"20169188","id":"","title":"Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development.","date":"2010","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/20169188","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["UPF1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":12143,"output_tokens":1362,"usd":0.028429,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6223,"output_tokens":2347,"usd":0.044895,"stage2_stop_reason":"end_turn"},"total_usd":0.073324,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2023,\n      \"finding\": \"PfUPF1 (PF3D7_1005500) localizes to puncta within the P. falciparum parasite cytoplasm, as determined by epitope-tagging via CRISPR-Cas9 and fluorescence microscopy.\",\n      \"method\": \"CRISPR-Cas9 epitope-tagging and fluorescence localization\",\n      \"journal\": \"mSphere\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — direct localization experiment with epitope tag, single lab, single method\",\n      \"pmids\": [\"37366629\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"PfUPF1 (PF3D7_1005500) physically interacts with PfUPF2 and other mRNA-binding proteins in P. falciparum, as shown by co-immunoprecipitation of epitope-tagged proteins.\",\n      \"method\": \"Co-immunoprecipitation of epitope-tagged PfUPF1 and PfUPF2\",\n      \"journal\": \"mSphere\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — reciprocal co-IP between UPF1 and UPF2 in a single lab study with two tagged proteins\",\n      \"pmids\": [\"37366629\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"CRISPR-Cas9 disruption of PfUPF1 (PF3D7_1005500) does not affect degradation of nonsense transcripts and does not impair parasite growth ex vivo, establishing that PfUPF1 is non-essential for NMD and for asexual blood-stage proliferation in P. falciparum.\",\n      \"method\": \"CRISPR-Cas9 knockout followed by RNA-seq and growth assays\",\n      \"journal\": \"mSphere\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean CRISPR KO with defined transcriptomic readout and growth phenotype, single lab, two orthogonal methods (RNA-seq + growth assay)\",\n      \"pmids\": [\"37366629\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"In P. yoelii zygotes (post-fertilization), UPF1 becomes associated with ALBA4 proximity proteomics signal, suggesting UPF1 enters or associates with the DOZI/CITH/ALBA regulatory mRNP complex in a stage-dependent manner in zygotes but not female gametocytes.\",\n      \"method\": \"TurboID-based proximity proteomics (BioID) of ALBA4 in P. yoelii female gametocytes and zygotes\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — proximity labeling (not direct Co-IP), single lab, UPF1 detected as proximal to ALBA4 only in zygotes\",\n      \"pmids\": [\"39777415\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium yoelii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2010,\n      \"finding\": \"In P. berghei female gametocytes, UPF1 homologs are encoded in the genome but are absent from affinity-purified DOZI/CITH mRNP complexes, indicating that UPF1 is NOT a component of the translational repression mRNP in gametocytes.\",\n      \"method\": \"Affinity purification coupled with mass-spectrometric analysis (negative result)\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — rigorous mass spectrometry-based interactome with explicit negative result, single lab\",\n      \"pmids\": [\"20169188\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"In P. falciparum, UPF1 (PF3D7_1005500) is expressed and localizes to cytoplasmic puncta where it interacts with PfUPF2 and other mRNA-binding proteins, including PfAlba4, but CRISPR-Cas9 knockout demonstrates it is non-essential for nonsense-mediated mRNA decay and for asexual blood-stage parasite growth; in P. yoelii zygotes (but not gametocytes), UPF1 associates with the ALBA4/DOZI/CITH translational regulatory complex in a stage-dependent manner, and in P. berghei gametocytes it is explicitly absent from this complex.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"UPF1 (PF3D7_1005500) is a cytoplasmic mRNA-associated protein of Plasmodium that, despite its annotation as the core NMD helicase, is dispensable for nonsense-mediated mRNA decay in P. falciparum blood-stage parasites [#2]. Epitope-tagging places PfUPF1 in cytoplasmic puncta, and reciprocal co-immunoprecipitation shows it physically associates with PfUPF2 and additional mRNA-binding proteins [#0, #1]. CRISPR-Cas9 disruption neither stabilizes nonsense transcripts nor impairs asexual proliferation, establishing that PfUPF1 is non-essential for both NMD and blood-stage growth [#2]. Its association with translational-regulatory mRNP complexes is stage- and species-dependent: in P. berghei female gametocytes UPF1 is explicitly excluded from affinity-purified DOZI/CITH mRNPs [#4], whereas in P. yoelii it becomes proximal to ALBA4 specifically in zygotes but not gametocytes [#3]. Beyond these findings, the enzymatic activity, RNA substrates, and mechanistic role of PF3D7_1005500 have not been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2010,\n      \"claim\": \"To test whether UPF1 participates in the gametocyte translational-repression machinery, its presence in the DOZI/CITH mRNP was assayed directly.\",\n      \"evidence\": \"affinity purification with mass spectrometry of DOZI/CITH complexes in P. berghei female gametocytes (negative result)\",\n      \"pmids\": [\"20169188\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Absence from the purified complex does not exclude transient or low-affinity association\",\n        \"Does not address UPF1 function at other life-cycle stages\",\n        \"No direct characterization of PF3D7_1005500 activity\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"To define where PfUPF1 acts and with whom, its subcellular distribution and protein partners were determined in P. falciparum.\",\n      \"evidence\": \"CRISPR-Cas9 epitope-tagging with fluorescence microscopy and reciprocal co-immunoprecipitation of PfUPF1 and PfUPF2\",\n      \"pmids\": [\"37366629\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Identity of the 'other mRNA-binding proteins' co-purifying with PfUPF1 not fully resolved\",\n        \"Whether the UPF1–UPF2 interaction is direct or bridged by RNA not established\",\n        \"Functional consequence of puncta localization unknown\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"To test the assumed NMD function, PfUPF1 was disrupted and both transcript degradation and growth were measured, revealing it is dispensable for canonical NMD.\",\n      \"evidence\": \"CRISPR-Cas9 knockout with RNA-seq and ex vivo growth assays in P. falciparum\",\n      \"pmids\": [\"37366629\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Does not identify the actual mRNA quality-control pathway operating in the parasite\",\n        \"Redundancy with a UPF1 paralog not ruled out\",\n        \"No stage-specific phenotype beyond asexual blood stages tested\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"To determine whether UPF1 joins translational-regulatory mRNPs during transmission stages, its proximity to ALBA4 was mapped across the female-gametocyte-to-zygote transition.\",\n      \"evidence\": \"TurboID proximity proteomics of ALBA4 in P. yoelii female gametocytes and zygotes\",\n      \"pmids\": [\"39777415\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Proximity labeling infers spatial vicinity, not direct interaction, and lacks reciprocal Co-IP validation\",\n        \"Functional role of UPF1 in zygotes not tested\",\n        \"Single lab, single method\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium yoelii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The catalytic activity, RNA substrates, and true cellular function of PF3D7_1005500 remain undefined, given that it is dispensable for canonical NMD.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No enzymatic or helicase activity demonstrated for PF3D7_1005500\",\n        \"No mRNA targets identified\",\n        \"Mechanistic basis for stage-dependent mRNP association unresolved\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003723\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005737\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0036464\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0000184\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"complexes\": [\n      \"PfUPF1–PfUPF2 mRNA-binding protein complex (P. falciparum) [#1]\",\n      \"ALBA4/DOZI/CITH regulatory mRNP — UPF1 proximal in P. yoelii zygotes [#3]; UPF1 excluded from DOZI/CITH mRNP in P. berghei gametocytes [#4]\"\n    ],\n    \"partners\": [\n      \"PfUPF2 [#1]\",\n      \"unidentified mRNA-binding proteins [#1]\",\n      \"ALBA4 (proximity, P. yoelii zygotes) [#3]\"\n    ],\n    \"other_free_text\": [\n      \"CRISPR-Cas9 disruption of PF3D7_1005500 does not stabilize nonsense transcripts and does not impair asexual blood-stage growth, so it is non-essential for NMD and proliferation [#2].\",\n      \"Association with translational-regulatory mRNPs is stage- and species-dependent: excluded in P. berghei gametocytes [#4], proximal to ALBA4 in P. yoelii zygotes [#3].\"\n    ]\n  }\n}\n```","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_1005500","name":null,"product":"regulator of nonsense transcripts 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1203700","name":null,"product":"regulator of nonsense transcripts 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0804200","name":null,"product":"regulator of nonsense transcripts 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"UPF1","evaluation":null}