{"gene":"PF3D7_0822900","run_date":"2026-09-08T12:51:27","timeline":{"discoveries":[{"year":2017,"finding":"PIC2 (PF3D7_0822900) was identified as a component of the PhIL1-interacting protein complex in the gametocyte inner membrane complex (IMC) of P. falciparum, revealed by immunoprecipitation of PhIL1 followed by mass spectrometry.","method":"Immunoprecipitation and mass spectrometry (co-IP/MS)","journal":"PLoS pathogens","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single Co-IP/MS experiment identifying PIC2 as a PhIL1-interacting candidate; no independent functional characterization of PIC2 itself reported in this abstract","pmids":["28985225"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2021,"finding":"PIC2 (PF3D7_0822900) was enriched in a BioID proximity-labeling proteomics screen using PhIL1 as bait in asexual blood-stage P. falciparum parasites, consistent with its membership in the IMC-associated PhIL1 complex.","method":"BioID proximity-dependent biotin identification followed by mass spectrometry","journal":"Cellular microbiology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — proximity-labeling proteomics identifies PIC2 as a candidate near PhIL1; no direct functional or localization experiment specific to PIC2 is described in this abstract","pmids":["33830607"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PIC2 (PF3D7_0822900) is established only as a member of the PhIL1-associated inner membrane complex in P. falciparum, identified by co-immunoprecipitation/mass spectrometry and BioID proximity labeling with PhIL1 as bait; no independent functional, structural, or localization data for PIC2 itself have been reported."},"narrative":{"mechanistic_narrative":"PIC2 (PF3D7_0822900) is characterized in the available corpus only as a candidate component of the PhIL1-interacting protein complex associated with the inner membrane complex (IMC) of Plasmodium falciparum, where it was identified by co-immunoprecipitation/mass spectrometry using PhIL1 as bait in gametocytes [PMID:28985225] and independently enriched in a BioID proximity-labeling screen with PhIL1 as bait in asexual blood-stage parasites [PMID:33830607]. Beyond this association with the PhIL1 complex, no independent functional, structural, or localization data specific to PIC2 have been characterized in the available corpus.","teleology":[{"year":2017,"claim":"To define the composition of the gametocyte IMC-associated PhIL1 complex, this work asked which proteins physically associate with PhIL1 and identified PIC2 as one of them.","evidence":"Immunoprecipitation of PhIL1 followed by mass spectrometry in P. falciparum gametocytes","pmids":["28985225"],"confidence":"Low","gaps":["Single Co-IP/MS experiment with no reciprocal pulldown using PIC2 as bait","No functional role established for PIC2 itself","No localization or structural data specific to PIC2"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2021,"claim":"To test whether the PhIL1 association extends to asexual stages and to corroborate the complex membership by an independent method, proximity labeling asked which proteins are near PhIL1 in blood-stage parasites, again recovering PIC2.","evidence":"BioID proximity-dependent biotin identification with PhIL1 as bait followed by mass spectrometry in asexual blood-stage P. falciparum","pmids":["33830607"],"confidence":"Low","gaps":["Proximity enrichment does not demonstrate direct binding to PhIL1","No PIC2-specific functional or localization experiment reported","Does not resolve whether PIC2 is a stable subunit or a transient neighbor"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The intrinsic molecular activity, subcellular localization, and functional contribution of PIC2 to the IMC remain undefined, as all evidence to date derives from PhIL1-centric interaction screens rather than direct study of PIC2.","evidence":"No direct functional, genetic, or structural characterization of PIC2 in the corpus","pmids":[],"confidence":"Low","gaps":["No gene knockout or knockdown phenotype for PIC2","No demonstration of direct PhIL1-PIC2 binding versus indirect complex membership","No structural model or domain-level functional assignment"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["PhIL1-interacting protein complex (IMC-associated)"],"partners":["PHIL1 (BAIT IN CO-IP/MS AND BIOID; PIC2 IDENTIFIED AS INTERACTING CANDIDATE)"],"other_free_text":["PIC2 (PF3D7_0822900) is established in the corpus solely as a candidate member of the PhIL1-associated inner membrane complex; no independent functional, structural, or localization data for PIC2 itself have been reported.","Identified in P. falciparum gametocytes by PhIL1 co-IP/MS [#0] and in asexual blood-stage parasites by PhIL1 BioID proximity labeling [#1]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"28985225","id":"PMC_28985225","title":"Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development.","date":"2017","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/28985225","citation_count":65,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PhIL1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"33830607","id":"PMC_33830607","title":"Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum.","date":"2021","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/33830607","citation_count":33,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PhIL1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"29138437","id":"PMC_29138437","title":"Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development.","date":"2017","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/29138437","citation_count":25,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PhIL1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34324609","id":"PMC_34324609","title":"Plasmodium falciparum PhIL1-associated complex plays an essential role in merozoite reorientation and invasion of host erythrocytes.","date":"2021","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/34324609","citation_count":10,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PhIL1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39576115","id":"PMC_39576115","title":"An inner membrane complex protein IMC1g in Plasmodium berghei is involved in asexual stage schizogony and parasite transmission.","date":"2024","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/39576115","citation_count":6,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PhIL1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":5487,"output_tokens":608,"usd":0.01279,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5179,"output_tokens":1442,"usd":0.030973,"stage2_stop_reason":"end_turn"},"total_usd":0.043763,"stage1_batch_id":"msgbatch_01DisAaTypwmC8QwdMBuePHo","stage2_batch_id":"msgbatch_0184zxZTqxiEfdYYfPMfkqjb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"PIC2 (PF3D7_0822900) was identified as a component of the PhIL1-interacting protein complex in the gametocyte inner membrane complex (IMC) of P. falciparum, revealed by immunoprecipitation of PhIL1 followed by mass spectrometry.\",\n      \"method\": \"Immunoprecipitation and mass spectrometry (co-IP/MS)\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single Co-IP/MS experiment identifying PIC2 as a PhIL1-interacting candidate; no independent functional characterization of PIC2 itself reported in this abstract\",\n      \"pmids\": [\"28985225\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"PIC2 (PF3D7_0822900) was enriched in a BioID proximity-labeling proteomics screen using PhIL1 as bait in asexual blood-stage P. falciparum parasites, consistent with its membership in the IMC-associated PhIL1 complex.\",\n      \"method\": \"BioID proximity-dependent biotin identification followed by mass spectrometry\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — proximity-labeling proteomics identifies PIC2 as a candidate near PhIL1; no direct functional or localization experiment specific to PIC2 is described in this abstract\",\n      \"pmids\": [\"33830607\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PIC2 (PF3D7_0822900) is established only as a member of the PhIL1-associated inner membrane complex in P. falciparum, identified by co-immunoprecipitation/mass spectrometry and BioID proximity labeling with PhIL1 as bait; no independent functional, structural, or localization data for PIC2 itself have been reported.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PIC2 (PF3D7_0822900) is characterized in the available corpus only as a candidate component of the PhIL1-interacting protein complex associated with the inner membrane complex (IMC) of Plasmodium falciparum, where it was identified by co-immunoprecipitation/mass spectrometry using PhIL1 as bait in gametocytes [#0] and independently enriched in a BioID proximity-labeling screen with PhIL1 as bait in asexual blood-stage parasites [#1]. Beyond this association with the PhIL1 complex, no independent functional, structural, or localization data specific to PIC2 have been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2017,\n      \"claim\": \"To define the composition of the gametocyte IMC-associated PhIL1 complex, this work asked which proteins physically associate with PhIL1 and identified PIC2 as one of them.\",\n      \"evidence\": \"Immunoprecipitation of PhIL1 followed by mass spectrometry in P. falciparum gametocytes\",\n      \"pmids\": [\"28985225\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single Co-IP/MS experiment with no reciprocal pulldown using PIC2 as bait\",\n        \"No functional role established for PIC2 itself\",\n        \"No localization or structural data specific to PIC2\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"To test whether the PhIL1 association extends to asexual stages and to corroborate the complex membership by an independent method, proximity labeling asked which proteins are near PhIL1 in blood-stage parasites, again recovering PIC2.\",\n      \"evidence\": \"BioID proximity-dependent biotin identification with PhIL1 as bait followed by mass spectrometry in asexual blood-stage P. falciparum\",\n      \"pmids\": [\"33830607\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Proximity enrichment does not demonstrate direct binding to PhIL1\",\n        \"No PIC2-specific functional or localization experiment reported\",\n        \"Does not resolve whether PIC2 is a stable subunit or a transient neighbor\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The intrinsic molecular activity, subcellular localization, and functional contribution of PIC2 to the IMC remain undefined, as all evidence to date derives from PhIL1-centric interaction screens rather than direct study of PIC2.\",\n      \"evidence\": \"No direct functional, genetic, or structural characterization of PIC2 in the corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No gene knockout or knockdown phenotype for PIC2\",\n        \"No demonstration of direct PhIL1-PIC2 binding versus indirect complex membership\",\n        \"No structural model or domain-level functional assignment\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0070258\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"PhIL1-interacting protein complex (IMC-associated)\"],\n    \"partners\": [\"PhIL1 (bait in co-IP/MS and BioID; PIC2 identified as interacting candidate)\"],\n    \"other_free_text\": [\n      \"PIC2 (PF3D7_0822900) is established in the corpus solely as a candidate member of the PhIL1-associated inner membrane complex; no independent functional, structural, or localization data for PIC2 itself have been reported.\",\n      \"Identified in P. falciparum gametocytes by PhIL1 co-IP/MS [#0] and in asexual blood-stage parasites by PhIL1 BioID proximity labeling [#1].\"\n    ]\n  }\n}","audit_flag":{"gene":"PF3D7_0822900","tier":"IDENTITY_CHECK","verdict":"identity unverified","subtype":"names_other_cards","uniprot_band":"","rules_fired":"R12","issue":"No cited paper names this card; 2 of 2 cited papers name other catalogue genes (PF3D7_0109000, PF3D7_1355600, PF3D7_1343700). The summary may describe one of those genes."},"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0822900","name":null,"product":"PhIL1-interacting candidate PIC2","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0707700","name":null,"product":"PhIL1-interacting candidate PIC2, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1316900","name":null,"product":"PhIL1-interacting candidate PIC2, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"PIC2","evaluation":null}