{"gene":"PF3D7_0723800","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2022,"finding":"AKIT1 (PF3D7_0723800) was identified as a component of a novel apicomplexan kinetochore sub-complex (AKiT1-6) positioned between CENP-C and the outer kinetochore, with cross-linked IP-MS establishing its membership in this inner kinetochore compartment. In Toxoplasma gondii, AKIT1 localization at kinetochores depends on CENP-C, but AKIT1 depletion did not affect Nuf2 levels, suggesting it is not required for outer kinetochore assembly.","method":"Cross-linked immunoprecipitation mass spectrometry (XL-IP-MS); interactomics; genetic depletion with kinetochore marker readout (Nuf2 levels)","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal cross-linked IP-MS with functional depletion follow-up, single lab, two orthogonal methods","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2023,"finding":"AKIT1 (PF3D7_0723800) localizes laterally to spindle microtubules throughout endomitosis during male gametogenesis in Plasmodium yoelii, as demonstrated by ultrastructure-expansion microscopy (U-ExM) and immunofluorescence assay using a double-tagged parasite strain (eb1::6HA; Akit1::4Myc). This lateral kinetochore-spindle attachment is EB1-dependent.","method":"Ultrastructure-expansion microscopy (U-ExM); immunofluorescence assay (IFA); endogenous tagging in double-tagged parasite strains; EB1 knockout with kinetochore localization readout","journal":"Nature communications","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct localization by U-ExM and IFA with functional consequence (EB1-dependence), single lab, two orthogonal imaging methods","pmids":["37208365"],"is_preprint":false,"attribution":[{"species":"Plasmodium yoelii","strain":""}]},{"year":2026,"finding":"AKIT1 (PF3D7_0723800) co-purifies with ARK1 in immunoprecipitation experiments from male-only Plasmodium berghei gametocytes (AKiT1-3xHA pulldowns compared alongside ARK1-3xHA pulldowns), placing AKIT1 within the ARK1-associated kinetochore interactome during male mitosis.","method":"Immunoprecipitation mass spectrometry (IP-MS) of AKiT1-3xHA and ARK1-3xHA from male-only gametocytes under native and low cross-linking conditions","journal":"Nature communications","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal tagged-IP from male-only gametocytes comparing AKiT1 and ARK1 pulldowns, single lab, limited replicates noted","pmids":["42323312"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2026,"finding":"AKIT1 (PF3D7_0723800) was co-enriched in ARK1-GFP pulldowns from both schizont and male gametocyte lysates in Plasmodium berghei, placing AKIT1 within the ARK1 interactome at both mitotic stages.","method":"Co-immunoprecipitation with anti-GFP from ARK1-GFP parasites followed by mass spectrometry; principal component analysis of pulldown data","journal":"Nature communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single co-IP/MS experiment with limited replicates (n=2), single lab, no orthogonal validation of AKIT1 specifically","pmids":["41748607"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"AKIT1 (PF3D7_0723800) is a component of an inner kinetochore sub-complex (AKiT1-6) in apicomplexan parasites, positioned between CENP-C and the outer kinetochore NDC80 complex; its kinetochore localization depends on CENP-C (shown in Toxoplasma gondii), it localizes laterally to spindle microtubules throughout endomitosis in an EB1-dependent manner during male gametogenesis (shown in Plasmodium yoelii), and it co-purifies with the Aurora-related kinase ARK1 during male mitosis in Plasmodium berghei."},"narrative":{"mechanistic_narrative":"AKIT1 (PF3D7_0723800) is a component of an inner kinetochore sub-complex (AKiT1-6) of apicomplexan parasites, positioned between the centromeric adaptor CENP-C and the outer kinetochore during parasite mitosis [PMID:36006241]. In Toxoplasma gondii, its recruitment to kinetochores depends on CENP-C, while its depletion does not reduce levels of the outer-kinetochore marker Nuf2, indicating it is not required for outer kinetochore assembly [PMID:36006241]. During male gametogenesis in Plasmodium yoelii, AKIT1 localizes laterally along spindle microtubules throughout endomitosis in an EB1-dependent manner, marking a lateral kinetochore-spindle attachment [PMID:37208365]. In Plasmodium berghei, AKIT1 co-purifies with the Aurora-related kinase ARK1 in male gametocytes and schizonts, placing it within the ARK1-associated kinetochore interactome during mitosis [PMID:42323312, PMID:41748607]. Beyond its membership in this inner kinetochore compartment and its physical association with CENP-C-dependent recruitment, EB1-dependent spindle localization, and ARK1, no further mechanistic detail on AKIT1 has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"Establishing that apicomplexan kinetochores contain a previously unrecognized inner sub-complex answered where AKIT1 sits in the kinetochore architecture and how it is recruited.","evidence":"Cross-linked IP-MS interactomics plus genetic depletion with Nuf2 readout in Toxoplasma gondii and Plasmodium berghei","pmids":["36006241"],"confidence":"Medium","gaps":["The molecular activity of AKIT1 within the AKiT1-6 complex is undefined","Direct binding interface between AKIT1 and CENP-C not resolved","Consequence of AKIT1 loss on chromosome segregation not measured"],"attribution":[{"species":"Toxoplasma gondii","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2023,"claim":"Live/expansion imaging during male gametogenesis showed that AKIT1-marked kinetochores attach laterally to spindle microtubules, defining the mode of kinetochore-spindle coupling and its dependence on EB1.","evidence":"U-ExM and IFA with double-tagged (eb1::6HA; Akit1::4Myc) parasites and EB1 knockout in Plasmodium yoelii","pmids":["37208365"],"confidence":"Medium","gaps":["Whether AKIT1 binds microtubules directly or via EB1 is not distinguished","Functional requirement of AKIT1 for lateral attachment not tested by AKIT1 depletion","Behavior in asexual (schizont) mitosis not addressed by this imaging"],"attribution":[{"species":"Plasmodium yoelii","strain":""}]},{"year":2026,"claim":"Reciprocal tagged pulldowns placed AKIT1 within the ARK1 kinase interactome during male mitosis, linking the inner kinetochore sub-complex to Aurora-related kinase signaling.","evidence":"IP-MS of AKiT1-3xHA and ARK1-3xHA from male-only Plasmodium berghei gametocytes under native and low cross-linking conditions","pmids":["42323312"],"confidence":"Medium","gaps":["Whether AKIT1 is a direct ARK1 substrate or a scaffold is unknown","Functional consequence of the AKIT1-ARK1 association not tested","Limited replicates noted for the pulldown"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2026,"claim":"AKIT1 co-enrichment in ARK1-GFP pulldowns from both schizonts and male gametocytes extended the ARK1 association across two distinct mitotic stages.","evidence":"Anti-GFP co-IP/MS from ARK1-GFP Plasmodium berghei parasites with PCA of pulldown data","pmids":["41748607"],"confidence":"Low","gaps":["Single co-IP/MS with limited replicates (n=2) and no orthogonal validation of AKIT1 specifically","Direct versus indirect nature of the AKIT1-ARK1 association unresolved","Stage-specific functional roles of AKIT1 not distinguished"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The molecular function of AKIT1 itself — its intrinsic activity, its direct binding partners within AKiT1-6, and whether it is required for chromosome segregation — remains unresolved beyond complex membership and physical associations.","evidence":"","pmids":[],"confidence":"Low","gaps":["No biochemical activity assigned to AKIT1","No structure or defined interaction interface for AKIT1","No AKIT1-specific loss-of-function phenotype for chromosome segregation reported"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["AKiT1-6 inner kinetochore sub-complex","ARK1-associated kinetochore interactome"],"partners":["CENP-C (RECRUITMENT DEPENDENCY, T. GONDII)","EB1 (LATERAL SPINDLE LOCALIZATION DEPENDENCY, P. YOELII)","ARK1 (CO-PURIFICATION, P. BERGHEI)"],"other_free_text":["Positioned between CENP-C and the outer kinetochore; AKIT1 depletion does not reduce Nuf2, so it is not required for outer kinetochore assembly (T. gondii) [#0]","Localizes laterally along spindle microtubules throughout endomitosis in male gametogenesis in an EB1-dependent manner (P. yoelii) [#1]","Associates with ARK1 in both schizonts and male gametocytes (P. berghei) [#2, #3]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":31,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42323312","id":"","title":"A modular chromosomal passenger complex rewires chromosome segregation in Plasmodium berghei.","date":"2026","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/42323312","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41748607","id":"","title":"Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex.","date":"2026","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/41748607","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"38987258","id":"","title":"Disruption of Plasmodium falciparum kinetochore proteins destabilises the nexus between the centrosome equivalent and the mitotic apparatus.","date":"2024","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/38987258","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37704606","id":"","title":"Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages.","date":"2023","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/37704606","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37208365","id":"","title":"EB1 decoration of microtubule lattice facilitates spindle-kinetochore lateral attachment in Plasmodium male gametogenesis.","date":"2023","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/37208365","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35800888","id":"","title":"Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.","date":"2022","source":"Frontiers in cell and developmental biology","url":"https://pubmed.ncbi.nlm.nih.gov/35800888","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT1"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":10364,"output_tokens":1403,"usd":0.026069,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6263,"output_tokens":2515,"usd":0.047095,"stage2_stop_reason":"end_turn"},"total_usd":0.073164,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"AKIT1 (PF3D7_0723800) was identified as a component of a novel apicomplexan kinetochore sub-complex (AKiT1-6) positioned between CENP-C and the outer kinetochore, with cross-linked IP-MS establishing its membership in this inner kinetochore compartment. In Toxoplasma gondii, AKIT1 localization at kinetochores depends on CENP-C, but AKIT1 depletion did not affect Nuf2 levels, suggesting it is not required for outer kinetochore assembly.\",\n      \"method\": \"Cross-linked immunoprecipitation mass spectrometry (XL-IP-MS); interactomics; genetic depletion with kinetochore marker readout (Nuf2 levels)\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal cross-linked IP-MS with functional depletion follow-up, single lab, two orthogonal methods\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"AKIT1 (PF3D7_0723800) localizes laterally to spindle microtubules throughout endomitosis during male gametogenesis in Plasmodium yoelii, as demonstrated by ultrastructure-expansion microscopy (U-ExM) and immunofluorescence assay using a double-tagged parasite strain (eb1::6HA; Akit1::4Myc). This lateral kinetochore-spindle attachment is EB1-dependent.\",\n      \"method\": \"Ultrastructure-expansion microscopy (U-ExM); immunofluorescence assay (IFA); endogenous tagging in double-tagged parasite strains; EB1 knockout with kinetochore localization readout\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization by U-ExM and IFA with functional consequence (EB1-dependence), single lab, two orthogonal imaging methods\",\n      \"pmids\": [\"37208365\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium yoelii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"AKIT1 (PF3D7_0723800) co-purifies with ARK1 in immunoprecipitation experiments from male-only Plasmodium berghei gametocytes (AKiT1-3xHA pulldowns compared alongside ARK1-3xHA pulldowns), placing AKIT1 within the ARK1-associated kinetochore interactome during male mitosis.\",\n      \"method\": \"Immunoprecipitation mass spectrometry (IP-MS) of AKiT1-3xHA and ARK1-3xHA from male-only gametocytes under native and low cross-linking conditions\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal tagged-IP from male-only gametocytes comparing AKiT1 and ARK1 pulldowns, single lab, limited replicates noted\",\n      \"pmids\": [\"42323312\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"AKIT1 (PF3D7_0723800) was co-enriched in ARK1-GFP pulldowns from both schizont and male gametocyte lysates in Plasmodium berghei, placing AKIT1 within the ARK1 interactome at both mitotic stages.\",\n      \"method\": \"Co-immunoprecipitation with anti-GFP from ARK1-GFP parasites followed by mass spectrometry; principal component analysis of pulldown data\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single co-IP/MS experiment with limited replicates (n=2), single lab, no orthogonal validation of AKIT1 specifically\",\n      \"pmids\": [\"41748607\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"AKIT1 (PF3D7_0723800) is a component of an inner kinetochore sub-complex (AKiT1-6) in apicomplexan parasites, positioned between CENP-C and the outer kinetochore NDC80 complex; its kinetochore localization depends on CENP-C (shown in Toxoplasma gondii), it localizes laterally to spindle microtubules throughout endomitosis in an EB1-dependent manner during male gametogenesis (shown in Plasmodium yoelii), and it co-purifies with the Aurora-related kinase ARK1 during male mitosis in Plasmodium berghei.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AKIT1 (PF3D7_0723800) is a component of an inner kinetochore sub-complex (AKiT1-6) of apicomplexan parasites, positioned between the centromeric adaptor CENP-C and the outer kinetochore during parasite mitosis [#0]. In Toxoplasma gondii, its recruitment to kinetochores depends on CENP-C, while its depletion does not reduce levels of the outer-kinetochore marker Nuf2, indicating it is not required for outer kinetochore assembly [#0]. During male gametogenesis in Plasmodium yoelii, AKIT1 localizes laterally along spindle microtubules throughout endomitosis in an EB1-dependent manner, marking a lateral kinetochore-spindle attachment [#1]. In Plasmodium berghei, AKIT1 co-purifies with the Aurora-related kinase ARK1 in male gametocytes and schizonts, placing it within the ARK1-associated kinetochore interactome during mitosis [#2, #3]. Beyond its membership in this inner kinetochore compartment and its physical association with CENP-C-dependent recruitment, EB1-dependent spindle localization, and ARK1, no further mechanistic detail on AKIT1 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Establishing that apicomplexan kinetochores contain a previously unrecognized inner sub-complex answered where AKIT1 sits in the kinetochore architecture and how it is recruited.\",\n      \"evidence\": \"Cross-linked IP-MS interactomics plus genetic depletion with Nuf2 readout in Toxoplasma gondii and Plasmodium berghei\",\n      \"pmids\": [\"36006241\"],\n      \"confidence\": \"Medium\",\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ],\n      \"gaps\": [\n        \"The molecular activity of AKIT1 within the AKiT1-6 complex is undefined\",\n        \"Direct binding interface between AKIT1 and CENP-C not resolved\",\n        \"Consequence of AKIT1 loss on chromosome segregation not measured\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Live/expansion imaging during male gametogenesis showed that AKIT1-marked kinetochores attach laterally to spindle microtubules, defining the mode of kinetochore-spindle coupling and its dependence on EB1.\",\n      \"evidence\": \"U-ExM and IFA with double-tagged (eb1::6HA; Akit1::4Myc) parasites and EB1 knockout in Plasmodium yoelii\",\n      \"pmids\": [\"37208365\"],\n      \"confidence\": \"Medium\",\n      \"attribution\": [\n        {\"species\": \"Plasmodium yoelii\", \"strain\": \"\"}\n      ],\n      \"gaps\": [\n        \"Whether AKIT1 binds microtubules directly or via EB1 is not distinguished\",\n        \"Functional requirement of AKIT1 for lateral attachment not tested by AKIT1 depletion\",\n        \"Behavior in asexual (schizont) mitosis not addressed by this imaging\"\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Reciprocal tagged pulldowns placed AKIT1 within the ARK1 kinase interactome during male mitosis, linking the inner kinetochore sub-complex to Aurora-related kinase signaling.\",\n      \"evidence\": \"IP-MS of AKiT1-3xHA and ARK1-3xHA from male-only Plasmodium berghei gametocytes under native and low cross-linking conditions\",\n      \"pmids\": [\"42323312\"],\n      \"confidence\": \"Medium\",\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ],\n      \"gaps\": [\n        \"Whether AKIT1 is a direct ARK1 substrate or a scaffold is unknown\",\n        \"Functional consequence of the AKIT1-ARK1 association not tested\",\n        \"Limited replicates noted for the pulldown\"\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"AKIT1 co-enrichment in ARK1-GFP pulldowns from both schizonts and male gametocytes extended the ARK1 association across two distinct mitotic stages.\",\n      \"evidence\": \"Anti-GFP co-IP/MS from ARK1-GFP Plasmodium berghei parasites with PCA of pulldown data\",\n      \"pmids\": [\"41748607\"],\n      \"confidence\": \"Low\",\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ],\n      \"gaps\": [\n        \"Single co-IP/MS with limited replicates (n=2) and no orthogonal validation of AKIT1 specifically\",\n        \"Direct versus indirect nature of the AKIT1-ARK1 association unresolved\",\n        \"Stage-specific functional roles of AKIT1 not distinguished\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function of AKIT1 itself — its intrinsic activity, its direct binding partners within AKiT1-6, and whether it is required for chromosome segregation — remains unresolved beyond complex membership and physical associations.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"attribution\": [],\n      \"gaps\": [\n        \"No biochemical activity assigned to AKIT1\",\n        \"No structure or defined interaction interface for AKIT1\",\n        \"No AKIT1-specific loss-of-function phenotype for chromosome segregation reported\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0000776\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0000940\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0005876\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0007059\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0007067\", \"supporting_discovery_ids\": [1, 2]}\n    ],\n    \"complexes\": [\n      \"AKiT1-6 inner kinetochore sub-complex\",\n      \"ARK1-associated kinetochore interactome\"\n    ],\n    \"partners\": [\n      \"CENP-C (recruitment dependency, T. gondii)\",\n      \"EB1 (lateral spindle localization dependency, P. yoelii)\",\n      \"ARK1 (co-purification, P. berghei)\"\n    ],\n    \"other_free_text\": [\n      \"Positioned between CENP-C and the outer kinetochore; AKIT1 depletion does not reduce Nuf2, so it is not required for outer kinetochore assembly (T. gondii) [#0]\",\n      \"Localizes laterally along spindle microtubules throughout endomitosis in male gametogenesis in an EB1-dependent manner (P. yoelii) [#1]\",\n      \"Associates with ARK1 in both schizonts and male gametocytes (P. berghei) [#2, #3]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0723800","name":null,"product":"apicomplexan kinetochore protein 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0621300","name":null,"product":"conserved Plasmodium protein, unknown function","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0319200","name":null,"product":"apicomplexan kinetochore protein 1, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AKIT1","evaluation":null}