{"gene":"PF3D7_0707600","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2022,"finding":"PF3D7_0707600 (MED10/PfMed10) was identified as a co-immunoprecipitated interaction partner of the conserved Mediator subunit PfMed31, placing MED10 within the P. falciparum Mediator complex. This is consistent with known yeast two-hybrid, co-IP, and structural data in other organisms showing Med31 specifically interacts with Med10.","method":"Coimmunoprecipitation of tagged PfMed31 followed by mass spectrometric analysis in blood-stage P. falciparum parasites","journal":"ACS omega","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — reciprocal Co-IP with MS identification, single lab, single experiment; establishes complex membership but no further functional characterization of MED10 itself","pmids":["35557691"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_0707600 (MED10) is a subunit of the Mediator complex in P. falciparum, identified through co-immunoprecipitation with the conserved Mediator subunit PfMed31 and mass spectrometry, but no further mechanistic details about its specific enzymatic activity, structural role, or individual function within the complex have been experimentally established."},"narrative":{"mechanistic_narrative":"PF3D7_0707600 (MED10) is a putative subunit of the Mediator complex in Plasmodium falciparum, identified through co-immunoprecipitation of the conserved Mediator subunit PfMed31 followed by mass spectrometry in blood-stage parasites [PMID:35557691]. This finding places MED10 within the parasite Mediator complex via its association with PfMed31 [PMID:35557691]. Beyond this complex-membership assignment, no specific enzymatic activity, structural role, or individual function of MED10 has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"Whether P. falciparum possesses a MED10 subunit associated with its Mediator complex was unknown; identifying MED10 as a PfMed31 interaction partner established its membership in the parasite Mediator complex.","evidence":"Co-immunoprecipitation of tagged PfMed31 followed by mass spectrometric identification in blood-stage P. falciparum parasites","pmids":["35557691"],"confidence":"Medium","gaps":["Interaction inferred from a single co-IP/MS experiment in one lab without reciprocal validation from MED10's side","No individual molecular function, enzymatic activity, or structural role of MED10 established","No data on MED10 localization, essentiality, or stage-specific behavior within the parasite"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The specific function of MED10 within the P. falciparum Mediator complex, its target genes, and its role in RNA polymerase II transcription remain uncharacterized.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No functional or genetic characterization of MED10 beyond complex membership","No structural model of MED10 or its interface within the Mediator complex","No evidence linking MED10 to specific transcriptional programs or parasite phenotypes"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Mediator complex (P. falciparum)"],"partners":["PFMED31"],"other_free_text":["PF3D7_0707600 (MED10) is established in the available corpus only as a co-immunoprecipitated component of the P. falciparum Mediator complex via association with PfMed31 [#0]; no individual function for MED10 itself has been experimentally characterized."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"31877193","id":"PMC_31877193","title":"Protein-RNA interactions important for Plasmodium transmission.","date":"2019","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/31877193","citation_count":16,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35557691","id":"","title":"Mediator Complex of the Malaria Parasite Plasmodium falciparum Associates with Evolutionarily Novel Subunits.","date":"2022","source":"ACS omega","url":"https://pubmed.ncbi.nlm.nih.gov/35557691","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["MED10"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3615,"output_tokens":400,"usd":0.008422,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4890,"output_tokens":995,"usd":0.024663,"stage2_stop_reason":"end_turn"},"total_usd":0.033085,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"PF3D7_0707600 (MED10/PfMed10) was identified as a co-immunoprecipitated interaction partner of the conserved Mediator subunit PfMed31, placing MED10 within the P. falciparum Mediator complex. This is consistent with known yeast two-hybrid, co-IP, and structural data in other organisms showing Med31 specifically interacts with Med10.\",\n      \"method\": \"Coimmunoprecipitation of tagged PfMed31 followed by mass spectrometric analysis in blood-stage P. falciparum parasites\",\n      \"journal\": \"ACS omega\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — reciprocal Co-IP with MS identification, single lab, single experiment; establishes complex membership but no further functional characterization of MED10 itself\",\n      \"pmids\": [\"35557691\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_0707600 (MED10) is a subunit of the Mediator complex in P. falciparum, identified through co-immunoprecipitation with the conserved Mediator subunit PfMed31 and mass spectrometry, but no further mechanistic details about its specific enzymatic activity, structural role, or individual function within the complex have been experimentally established.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0707600 (MED10) is a putative subunit of the Mediator complex in Plasmodium falciparum, identified through co-immunoprecipitation of the conserved Mediator subunit PfMed31 followed by mass spectrometry in blood-stage parasites [#0]. This finding places MED10 within the parasite Mediator complex via its association with PfMed31 [#0]. Beyond this complex-membership assignment, no specific enzymatic activity, structural role, or individual function of MED10 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Whether P. falciparum possesses a MED10 subunit associated with its Mediator complex was unknown; identifying MED10 as a PfMed31 interaction partner established its membership in the parasite Mediator complex.\",\n      \"evidence\": \"Co-immunoprecipitation of tagged PfMed31 followed by mass spectrometric identification in blood-stage P. falciparum parasites\",\n      \"pmids\": [\n        \"35557691\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Interaction inferred from a single co-IP/MS experiment in one lab without reciprocal validation from MED10's side\",\n        \"No individual molecular function, enzymatic activity, or structural role of MED10 established\",\n        \"No data on MED10 localization, essentiality, or stage-specific behavior within the parasite\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific function of MED10 within the P. falciparum Mediator complex, its target genes, and its role in RNA polymerase II transcription remain uncharacterized.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional or genetic characterization of MED10 beyond complex membership\",\n        \"No structural model of MED10 or its interface within the Mediator complex\",\n        \"No evidence linking MED10 to specific transcriptional programs or parasite phenotypes\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Mediator complex (P. falciparum)\"\n    ],\n    \"partners\": [\n      \"PfMed31\"\n    ],\n    \"other_free_text\": [\n      \"PF3D7_0707600 (MED10) is established in the available corpus only as a co-immunoprecipitated component of the P. falciparum Mediator complex via association with PfMed31 [#0]; no individual function for MED10 itself has been experimentally characterized.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0707600","name":null,"product":"mediator of RNA polymerase II transcription subunit 10, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0804800","name":null,"product":"mediator of RNA polymerase II transcription subunit 10, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0106100","name":null,"product":"mediator of RNA polymerase II transcription subunit 10, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"MED10","evaluation":null}