{"gene":"PF3D7_0706300","run_date":"2026-09-08T12:51:27","timeline":{"discoveries":[{"year":2022,"finding":"AKIT11 (PF3D7_0706300) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, establishing its membership in the apicomplexan kinetochore complex.","method":"Interactomics (co-immunoprecipitation/pulldown) combined with deep homology searches","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — identified via interactomics screen in a single study; membership in the kinetochore complex is supported by multiple orthogonal methods (interactomics + homology + localization) within one lab","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"AKIT11 (PF3D7_0706300) is an apicomplexan kinetochore protein identified as a component of the divergent apicomplexan kinetochore complex, which organizes into at least four discrete subkinetochore compartments and mediates chromosome segregation, but no further mechanistic detail specific to AKIT11's function or interactions has been established beyond its membership in this complex."},"narrative":{"mechanistic_narrative":"AKIT11 (PF3D7_0706300) is a component of the divergent apicomplexan kinetochore complex, identified as one of 13 previously unknown kinetochore proteins in Apicomplexa through interactomics combined with deep homology searches in Plasmodium falciparum [PMID:36006241]. Beyond its membership in this complex, no mechanistic detail specific to AKIT11's own molecular function, interaction partners, or localization has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"The composition of the highly divergent apicomplexan kinetochore was largely unknown; AKIT11 was placed within this complex, establishing that PF3D7_0706300 is a bona fide kinetochore component rather than an uncharacterized ORF.","evidence":"Interactomics (co-immunoprecipitation/pulldown) combined with deep homology searches in P. falciparum","pmids":["36006241"],"confidence":"Medium","gaps":["The specific molecular activity of AKIT11 within the kinetochore is undefined","Direct binding partners and its position among subkinetochore compartments are not resolved","No functional/loss-of-function phenotype for AKIT11 itself has been reported"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"What AKIT11 contributes mechanistically to kinetochore assembly, chromosome attachment, or segregation — as distinct from the complex as a whole — remains unestablished.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No direct partner or substrate identified for AKIT11","No structural model or defined subkinetochore assignment","No essentiality or depletion phenotype specific to AKIT11"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["apicomplexan kinetochore complex"],"partners":[],"other_free_text":["Identified as one of 13 previously unknown apicomplexan kinetochore components via interactomics and deep homology searches in P. falciparum [#0]","No mechanistic detail specific to AKIT11 established beyond its membership in the kinetochore complex"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":31,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3258,"output_tokens":371,"usd":0.00767,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4847,"output_tokens":919,"usd":0.023605,"stage2_stop_reason":"end_turn"},"total_usd":0.031275,"stage1_batch_id":"msgbatch_01DisAaTypwmC8QwdMBuePHo","stage2_batch_id":"msgbatch_0184zxZTqxiEfdYYfPMfkqjb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"AKIT11 (PF3D7_0706300) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, establishing its membership in the apicomplexan kinetochore complex.\",\n      \"method\": \"Interactomics (co-immunoprecipitation/pulldown) combined with deep homology searches\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — identified via interactomics screen in a single study; membership in the kinetochore complex is supported by multiple orthogonal methods (interactomics + homology + localization) within one lab\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"AKIT11 (PF3D7_0706300) is an apicomplexan kinetochore protein identified as a component of the divergent apicomplexan kinetochore complex, which organizes into at least four discrete subkinetochore compartments and mediates chromosome segregation, but no further mechanistic detail specific to AKIT11's function or interactions has been established beyond its membership in this complex.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AKIT11 (PF3D7_0706300) is a component of the divergent apicomplexan kinetochore complex, identified as one of 13 previously unknown kinetochore proteins in Apicomplexa through interactomics combined with deep homology searches in Plasmodium falciparum [#0]. Beyond its membership in this complex, no mechanistic detail specific to AKIT11's own molecular function, interaction partners, or localization has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"The composition of the highly divergent apicomplexan kinetochore was largely unknown; AKIT11 was placed within this complex, establishing that PF3D7_0706300 is a bona fide kinetochore component rather than an uncharacterized ORF.\",\n      \"evidence\": \"Interactomics (co-immunoprecipitation/pulldown) combined with deep homology searches in P. falciparum\",\n      \"pmids\": [\"36006241\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The specific molecular activity of AKIT11 within the kinetochore is undefined\",\n        \"Direct binding partners and its position among subkinetochore compartments are not resolved\",\n        \"No functional/loss-of-function phenotype for AKIT11 itself has been reported\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"What AKIT11 contributes mechanistically to kinetochore assembly, chromosome attachment, or segregation — as distinct from the complex as a whole — remains unestablished.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct partner or substrate identified for AKIT11\",\n        \"No structural model or defined subkinetochore assignment\",\n        \"No essentiality or depletion phenotype specific to AKIT11\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [\"apicomplexan kinetochore complex\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as one of 13 previously unknown apicomplexan kinetochore components via interactomics and deep homology searches in P. falciparum [#0]\",\n      \"No mechanistic detail specific to AKIT11 established beyond its membership in the kinetochore complex\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0706300","name":null,"product":"apicomplexan kinetochore protein 11, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AKIT11","evaluation":null}