{"gene":"PF3D7_0618300","run_date":"2026-09-08T12:51:26","timeline":{"discoveries":[{"year":2014,"finding":"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution revealed the molecular architecture of the ribosome including its constituent ribosomal proteins; L27a (PF3D7_0618300) is a component of this ribosome as visualized in the structural map, establishing its membership in the cytoplasmic 80S ribosome complex.","method":"Cryo-EM structure determination at 3.2 Å resolution","journal":"eLife","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — high-resolution cryo-EM structure establishes ribosome membership but the abstract does not describe specific functional experiments on L27a itself; membership inferred from the overall ribosome structure","pmids":["24913268"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2015,"finding":"Cryo-EM reconstructions of P. falciparum blood-stage ribosome at sub-nanometer resolution with atomic model building confirmed L27a (PF3D7_0618300) as part of the cytoplasmic 80S ribosome purified from schizonts, and captured multiple conformational (translational) states of the ribosome.","method":"Cryo-EM reconstruction and flexible fitting of atomic models from ribosomes purified from P. falciparum schizonts","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — rigorous structural method applied to native parasite ribosomes, but no specific functional experiment on L27a itself; membership established indirectly through the overall structure","pmids":["26432834"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"finding":"In situ cryo-electron tomography of P. falciparum-infected erythrocytes determined eight high-resolution structures of the native malarial ribosome in actively translating states inside the cell, establishing L27a (PF3D7_0618300) as a component of the translationally active 80S ribosome in its native cellular context; perturbation with a Plasmodium-specific translation inhibitor decreased elongation factor-bound ribosomal states and triggered apparent upregulation of ribosome biogenesis.","method":"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes","journal":"Nature structural & molecular biology","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — state-of-the-art in situ structural method in native cellular context, but the abstract describes ribosome-level findings without specific experiments on L27a individually","pmids":["40825879"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_0618300 (L27a) is established as a structural component of the cytoplasmic P. falciparum 80S ribosome, as visualized by multiple cryo-EM and cryo-ET studies; beyond its membership in the ribosome complex, no specific mechanistic function, binding partners, or post-translational modifications have been experimentally defined for L27a itself in the available literature."},"narrative":{"mechanistic_narrative":"PF3D7_0618300 (L27a) is a structural constituent of the cytoplasmic 80S ribosome of Plasmodium falciparum, identified within the molecular architecture of the ribosome resolved by cryo-EM at 3.2 Å [PMID:24913268] and confirmed as a component of native ribosomes purified from schizonts that occupy multiple translational conformational states [PMID:26432834]. In situ cryo-electron tomography places L27a within translationally active 80S ribosomes in their native cellular context inside infected erythrocytes [PMID:40825879]. Beyond this membership in the cytoplasmic ribosome, no specific mechanistic function, binding partners, or post-translational modifications have been experimentally defined for L27a itself in the available corpus; every finding attributes L27a only as one component of the larger ribosome complex.","teleology":[{"year":2014,"claim":"Whether L27a was part of the P. falciparum cytoplasmic ribosome was resolved by placing it within the high-resolution architecture of the assembled 80S particle.","evidence":"Cryo-EM structure determination of the P. falciparum 80S ribosome at 3.2 Å resolution","pmids":["24913268"],"confidence":"Medium","gaps":["Membership is inferred from the overall ribosome map; no functional experiment on L27a itself","No binding partners or post-translational modifications defined specifically for L27a","No role in ribosome assembly or translation attributed to L27a individually"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2015,"claim":"It was unclear whether L27a occupied the ribosome across functional states; capturing native schizont ribosomes in multiple translational conformations confirmed L27a as a stable component of the working particle.","evidence":"Cryo-EM reconstruction and flexible atomic-model fitting of ribosomes purified from P. falciparum schizonts","pmids":["26432834"],"confidence":"Medium","gaps":["No functional experiment isolating L27a's contribution to the conformational cycle","Whether L27a movement participates in specific translation steps is unaddressed","No interaction map for L27a within the complex"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"claim":"Whether L27a is present in ribosomes actively translating in the living cell was answered by resolving native ribosome states in situ, establishing L27a as part of the translationally active 80S ribosome in its cellular context.","evidence":"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes, with translation-inhibitor perturbation","pmids":["40825879"],"confidence":"Medium","gaps":["The inhibitor and biogenesis findings are ribosome-level; no L27a-specific perturbation performed","No individual functional or regulatory role assigned to L27a","Post-translational modifications of L27a remain undefined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"It remains unknown whether L27a has any function, interaction, or regulatory role distinct from its structural membership in the 80S ribosome.","evidence":"No experiment in the corpus isolates or perturbs L27a individually","pmids":[],"confidence":"Medium","gaps":["No genetic knockdown or knockout of L27a reported","No direct binding partners or substrates identified for L27a","No post-translational modification or regulatory mechanism defined"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Cytoplasmic 80S ribosome"],"partners":[],"other_free_text":["L27a (PF3D7_0618300) is documented in the corpus only as a structural component of the P. falciparum cytoplasmic 80S ribosome; no function, binding partner, or modification specific to L27a itself has been experimentally established.","All findings derive from ribosome-level structural studies (cryo-EM and in situ cryo-ET) in P. falciparum; the corpus is dominated by the ribosome complex as a whole rather than L27a individually."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"24913268","id":"PMC_24913268","title":"Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.","date":"2014","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/24913268","citation_count":272,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"28288098","id":"PMC_28288098","title":"Mefloquine targets the Plasmodium falciparum 80S ribosome to inhibit protein synthesis.","date":"2017","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/28288098","citation_count":136,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"26432834","id":"PMC_26432834","title":"Dynamical features of the Plasmodium falciparum ribosome during translation.","date":"2015","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/26432834","citation_count":45,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"20078859","id":"PMC_20078859","title":"Assessing functional annotation transfers with inter-species conserved coexpression: application to Plasmodium falciparum.","date":"2010","source":"BMC genomics","url":"https://pubmed.ncbi.nlm.nih.gov/20078859","citation_count":12,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40825879","id":"PMC_40825879","title":"Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug.","date":"2025","source":"Nature structural & molecular biology","url":"https://pubmed.ncbi.nlm.nih.gov/40825879","citation_count":7,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40480224","id":"PMC_40480224","title":"Antimalarial drug artemisinin stabilizes PfRACK1 binding to the ribosome.","date":"2025","source":"Structure (London, England : 1993)","url":"https://pubmed.ncbi.nlm.nih.gov/40480224","citation_count":3,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":5349,"output_tokens":939,"usd":0.015066,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5657,"output_tokens":1809,"usd":0.036755,"stage2_stop_reason":"end_turn"},"total_usd":0.051821,"stage1_batch_id":"msgbatch_01DisAaTypwmC8QwdMBuePHo","stage2_batch_id":"msgbatch_0184zxZTqxiEfdYYfPMfkqjb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2014,\n      \"finding\": \"Cryo-EM structure of the P. falciparum 80S ribosome at 3.2 Å resolution revealed the molecular architecture of the ribosome including its constituent ribosomal proteins; L27a (PF3D7_0618300) is a component of this ribosome as visualized in the structural map, establishing its membership in the cytoplasmic 80S ribosome complex.\",\n      \"method\": \"Cryo-EM structure determination at 3.2 Å resolution\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — high-resolution cryo-EM structure establishes ribosome membership but the abstract does not describe specific functional experiments on L27a itself; membership inferred from the overall ribosome structure\",\n      \"pmids\": [\"24913268\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2015,\n      \"finding\": \"Cryo-EM reconstructions of P. falciparum blood-stage ribosome at sub-nanometer resolution with atomic model building confirmed L27a (PF3D7_0618300) as part of the cytoplasmic 80S ribosome purified from schizonts, and captured multiple conformational (translational) states of the ribosome.\",\n      \"method\": \"Cryo-EM reconstruction and flexible fitting of atomic models from ribosomes purified from P. falciparum schizonts\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — rigorous structural method applied to native parasite ribosomes, but no specific functional experiment on L27a itself; membership established indirectly through the overall structure\",\n      \"pmids\": [\"26432834\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"In situ cryo-electron tomography of P. falciparum-infected erythrocytes determined eight high-resolution structures of the native malarial ribosome in actively translating states inside the cell, establishing L27a (PF3D7_0618300) as a component of the translationally active 80S ribosome in its native cellular context; perturbation with a Plasmodium-specific translation inhibitor decreased elongation factor-bound ribosomal states and triggered apparent upregulation of ribosome biogenesis.\",\n      \"method\": \"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes\",\n      \"journal\": \"Nature structural & molecular biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — state-of-the-art in situ structural method in native cellular context, but the abstract describes ribosome-level findings without specific experiments on L27a individually\",\n      \"pmids\": [\"40825879\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"PF3D7_0618300 (L27a) is established as a structural component of the cytoplasmic P. falciparum 80S ribosome, as visualized by multiple cryo-EM and cryo-ET studies; beyond its membership in the ribosome complex, no specific mechanistic function, binding partners, or post-translational modifications have been experimentally defined for L27a itself in the available literature.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0618300 (L27a) is a structural constituent of the cytoplasmic 80S ribosome of Plasmodium falciparum, identified within the molecular architecture of the ribosome resolved by cryo-EM at 3.2 Å [#0] and confirmed as a component of native ribosomes purified from schizonts that occupy multiple translational conformational states [#1]. In situ cryo-electron tomography places L27a within translationally active 80S ribosomes in their native cellular context inside infected erythrocytes [#2]. Beyond this membership in the cytoplasmic ribosome, no specific mechanistic function, binding partners, or post-translational modifications have been experimentally defined for L27a itself in the available corpus; every finding attributes L27a only as one component of the larger ribosome complex.\",\n  \"teleology\": [\n    {\n      \"year\": 2014,\n      \"claim\": \"Whether L27a was part of the P. falciparum cytoplasmic ribosome was resolved by placing it within the high-resolution architecture of the assembled 80S particle.\",\n      \"evidence\": \"Cryo-EM structure determination of the P. falciparum 80S ribosome at 3.2 Å resolution\",\n      \"pmids\": [\"24913268\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Membership is inferred from the overall ribosome map; no functional experiment on L27a itself\",\n        \"No binding partners or post-translational modifications defined specifically for L27a\",\n        \"No role in ribosome assembly or translation attributed to L27a individually\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2015,\n      \"claim\": \"It was unclear whether L27a occupied the ribosome across functional states; capturing native schizont ribosomes in multiple translational conformations confirmed L27a as a stable component of the working particle.\",\n      \"evidence\": \"Cryo-EM reconstruction and flexible atomic-model fitting of ribosomes purified from P. falciparum schizonts\",\n      \"pmids\": [\"26432834\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional experiment isolating L27a's contribution to the conformational cycle\",\n        \"Whether L27a movement participates in specific translation steps is unaddressed\",\n        \"No interaction map for L27a within the complex\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Whether L27a is present in ribosomes actively translating in the living cell was answered by resolving native ribosome states in situ, establishing L27a as part of the translationally active 80S ribosome in its cellular context.\",\n      \"evidence\": \"In situ cryo-electron tomography inside P. falciparum-infected human erythrocytes, with translation-inhibitor perturbation\",\n      \"pmids\": [\"40825879\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The inhibitor and biogenesis findings are ribosome-level; no L27a-specific perturbation performed\",\n        \"No individual functional or regulatory role assigned to L27a\",\n        \"Post-translational modifications of L27a remain undefined\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown whether L27a has any function, interaction, or regulatory role distinct from its structural membership in the 80S ribosome.\",\n      \"evidence\": \"No experiment in the corpus isolates or perturbs L27a individually\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No genetic knockdown or knockout of L27a reported\",\n        \"No direct binding partners or substrates identified for L27a\",\n        \"No post-translational modification or regulatory mechanism defined\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003735\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005737\", \"supporting_discovery_ids\": [0, 1, 2]},\n      {\"term_id\": \"GO:0005840\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006412\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"complexes\": [\n      \"Cytoplasmic 80S ribosome\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"L27a (PF3D7_0618300) is documented in the corpus only as a structural component of the P. falciparum cytoplasmic 80S ribosome; no function, binding partner, or modification specific to L27a itself has been experimentally established.\",\n      \"All findings derive from ribosome-level structural studies (cryo-EM and in situ cryo-ET) in P. falciparum; the corpus is dominated by the ribosome complex as a whole rather than L27a individually.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0618300","name":null,"product":"60S ribosomal protein L27a, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1117500","name":null,"product":"ribosomal protein L27a, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1132100","name":null,"product":"60S ribosomal protein L27a, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"","evaluation":null}