{"gene":"PF3D7_0520200","run_date":"2026-09-08T19:44:17","timeline":{"discoveries":[{"year":2022,"finding":"PF3D7_0520200 (MED17/PfMed17) was physically identified as an interaction partner of the conserved Mediator subunit PfMed31 by coimmunoprecipitation and mass spectrometry, establishing its membership in the P. falciparum Mediator complex. The body text notes that MED17 (head module) connects the head and middle modules via a Med17–Med21 interaction conserved from yeast.","method":"Coimmunoprecipitation (Co-IP) of PfMed31 followed by mass spectrometry","journal":"ACS omega","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — reciprocal Co-IP with MS in a single study identifying MED17 as a PfMed31 partner; single lab, single method","pmids":["35557691"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"finding":"PF3D7_0520200 (MED17) was identified as part of the PfSET10 nuclear interaction network (mediators of RNA polymerase II) via TurboID proximity labeling coupled with mass spectrometry, placing it in a chromatin modulation network active during P. falciparum blood-stage intraerythrocytic development.","method":"TurboID proximity labeling coupled with mass spectrometry","journal":"mSphere","confidence":"Low","confidence_rationale":"Tier 3 / Weak — proximity labeling (not direct Co-IP) in a single study; MED17 is listed among many interactors of PfSET10 with no functional follow-up specific to MED17","pmids":["39445823"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_0520200 (MED17) is a subunit of the P. falciparum Mediator complex, physically associated with the conserved subunit PfMed31 and implicated in bridging the head and middle modules of the complex, and it also appears in the nuclear interaction network of the histone methyltransferase PfSET10, but no direct enzymatic activity, structure, or further mechanistic characterization specific to MED17 has been established beyond its complex membership."},"narrative":{"mechanistic_narrative":"PF3D7_0520200 (MED17) is a subunit of the Plasmodium falciparum Mediator complex, the coactivator module associated with RNA polymerase II transcription [PMID:35557691]. It was physically identified as an interaction partner of the conserved Mediator subunit PfMed31 by reciprocal coimmunoprecipitation and mass spectrometry, establishing its membership in the P. falciparum Mediator complex, with the head-module MED17 implicated in bridging the head and middle modules via a Med17–Med21 interaction conserved from yeast [PMID:35557691]. Beyond its complex membership, no direct enzymatic activity, structure, or dedicated functional characterization of MED17 itself has been established in the available corpus; its only additional appearance is as one member of the PfSET10 nuclear interaction network during blood-stage development [PMID:39445823].","teleology":[{"year":2022,"claim":"Whether P. falciparum encodes a functional Mediator complex and which subunits associate was unresolved; identifying MED17 as a PfMed31 partner established that PF3D7_0520200 is a bona fide Mediator subunit rather than a merely annotated ortholog.","evidence":"Reciprocal coimmunoprecipitation of PfMed31 followed by mass spectrometry in P. falciparum","pmids":["35557691"],"confidence":"Medium","gaps":["Single lab, single method; no reciprocal Co-IP starting from MED17 itself","The proposed Med17-Med21 head-to-middle bridging role is inferred from yeast conservation, not directly demonstrated in P. falciparum","No functional consequence of MED17 loss on transcription was tested"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"claim":"Whether MED17 participates in chromatin-associated regulatory networks beyond core Mediator was unknown; proximity labeling placed it in the PfSET10 nuclear network during intraerythrocytic development.","evidence":"TurboID proximity labeling coupled with mass spectrometry in P. falciparum blood stages","pmids":["39445823"],"confidence":"Low","gaps":["Proximity labeling reflects spatial neighborhood, not direct interaction; MED17 is one of many listed interactors with no MED17-specific follow-up","No evidence that MED17 is functionally required for PfSET10 activity or vice versa","Stage- and locus-specificity of any MED17 role remains uncharacterized"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"The direct molecular function of MED17, its structural role within the Mediator complex, and any phenotype from its perturbation in P. falciparum remain unestablished.","evidence":"","pmids":[],"confidence":"Low","gaps":["No genetic knockdown/knockout of MED17 to define its contribution to RNA Pol II transcription","No structural or biochemical validation of the head-to-middle module bridging role in P. falciparum","No target genes or transcriptional programs assigned to MED17"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Mediator complex (P. falciparum)"],"partners":["PFMED31 (PF3D7 MEDIATOR SUBUNIT)","PFSET10 (HISTONE METHYLTRANSFERASE; PROXIMITY-NETWORK ASSOCIATION)"],"other_free_text":["MED17 assigned to the Mediator head module; head-to-middle module bridging via a Med17-Med21 interaction is inferred from yeast conservation, not directly demonstrated in P. falciparum [#0].","The PfSET10 network association derives from TurboID proximity labeling (Low confidence), not direct Co-IP, and carries no MED17-specific functional follow-up [#1]."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"4971014","id":"PMC_4971014","title":"DNA, RNA , and protein synthesis in erythrocytic forms of Plasmodium knowlesi.","date":"1968","source":"The American journal of tropical medicine and hygiene","url":"https://pubmed.ncbi.nlm.nih.gov/4971014","citation_count":56,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"31877193","id":"PMC_31877193","title":"Protein-RNA interactions important for Plasmodium transmission.","date":"2019","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/31877193","citation_count":16,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39445823","id":"","title":"The Plasmodium falciparum histone methyltransferase SET10 participates in a chromatin modulation network crucial for intraerythrocytic development.","date":"2024","source":"mSphere","url":"https://pubmed.ncbi.nlm.nih.gov/39445823","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["MED17"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35557691","id":"","title":"Mediator Complex of the Malaria Parasite Plasmodium falciparum Associates with Evolutionarily Novel Subunits.","date":"2022","source":"ACS omega","url":"https://pubmed.ncbi.nlm.nih.gov/35557691","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["MED17"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":4584,"output_tokens":677,"usd":0.011954,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5259,"output_tokens":1582,"usd":0.032923,"stage2_stop_reason":"end_turn"},"total_usd":0.044877,"stage1_batch_id":"msgbatch_01SRp5hyRc82zNjddxyCCXGk","stage2_batch_id":"msgbatch_01K3BTmBazRo3pRrMvyaPMTY","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"PF3D7_0520200 (MED17/PfMed17) was physically identified as an interaction partner of the conserved Mediator subunit PfMed31 by coimmunoprecipitation and mass spectrometry, establishing its membership in the P. falciparum Mediator complex. The body text notes that MED17 (head module) connects the head and middle modules via a Med17–Med21 interaction conserved from yeast.\",\n      \"method\": \"Coimmunoprecipitation (Co-IP) of PfMed31 followed by mass spectrometry\",\n      \"journal\": \"ACS omega\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — reciprocal Co-IP with MS in a single study identifying MED17 as a PfMed31 partner; single lab, single method\",\n      \"pmids\": [\"35557691\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"PF3D7_0520200 (MED17) was identified as part of the PfSET10 nuclear interaction network (mediators of RNA polymerase II) via TurboID proximity labeling coupled with mass spectrometry, placing it in a chromatin modulation network active during P. falciparum blood-stage intraerythrocytic development.\",\n      \"method\": \"TurboID proximity labeling coupled with mass spectrometry\",\n      \"journal\": \"mSphere\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — proximity labeling (not direct Co-IP) in a single study; MED17 is listed among many interactors of PfSET10 with no functional follow-up specific to MED17\",\n      \"pmids\": [\"39445823\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_0520200 (MED17) is a subunit of the P. falciparum Mediator complex, physically associated with the conserved subunit PfMed31 and implicated in bridging the head and middle modules of the complex, and it also appears in the nuclear interaction network of the histone methyltransferase PfSET10, but no direct enzymatic activity, structure, or further mechanistic characterization specific to MED17 has been established beyond its complex membership.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0520200 (MED17) is a subunit of the Plasmodium falciparum Mediator complex, the coactivator module associated with RNA polymerase II transcription [#0]. It was physically identified as an interaction partner of the conserved Mediator subunit PfMed31 by reciprocal coimmunoprecipitation and mass spectrometry, establishing its membership in the P. falciparum Mediator complex, with the head-module MED17 implicated in bridging the head and middle modules via a Med17–Med21 interaction conserved from yeast [#0]. Beyond its complex membership, no direct enzymatic activity, structure, or dedicated functional characterization of MED17 itself has been established in the available corpus; its only additional appearance is as one member of the PfSET10 nuclear interaction network during blood-stage development [#1].\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Whether P. falciparum encodes a functional Mediator complex and which subunits associate was unresolved; identifying MED17 as a PfMed31 partner established that PF3D7_0520200 is a bona fide Mediator subunit rather than a merely annotated ortholog.\",\n      \"evidence\": \"Reciprocal coimmunoprecipitation of PfMed31 followed by mass spectrometry in P. falciparum\",\n      \"pmids\": [\"35557691\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Single lab, single method; no reciprocal Co-IP starting from MED17 itself\",\n        \"The proposed Med17-Med21 head-to-middle bridging role is inferred from yeast conservation, not directly demonstrated in P. falciparum\",\n        \"No functional consequence of MED17 loss on transcription was tested\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Whether MED17 participates in chromatin-associated regulatory networks beyond core Mediator was unknown; proximity labeling placed it in the PfSET10 nuclear network during intraerythrocytic development.\",\n      \"evidence\": \"TurboID proximity labeling coupled with mass spectrometry in P. falciparum blood stages\",\n      \"pmids\": [\"39445823\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Proximity labeling reflects spatial neighborhood, not direct interaction; MED17 is one of many listed interactors with no MED17-specific follow-up\",\n        \"No evidence that MED17 is functionally required for PfSET10 activity or vice versa\",\n        \"Stage- and locus-specificity of any MED17 role remains uncharacterized\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct molecular function of MED17, its structural role within the Mediator complex, and any phenotype from its perturbation in P. falciparum remain unestablished.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No genetic knockdown/knockout of MED17 to define its contribution to RNA Pol II transcription\",\n        \"No structural or biochemical validation of the head-to-middle module bridging role in P. falciparum\",\n        \"No target genes or transcriptional programs assigned to MED17\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006357\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"complexes\": [\n      \"Mediator complex (P. falciparum)\"\n    ],\n    \"partners\": [\n      \"PfMed31 (PF3D7 Mediator subunit)\",\n      \"PfSET10 (histone methyltransferase; proximity-network association)\"\n    ],\n    \"other_free_text\": [\n      \"MED17 assigned to the Mediator head module; head-to-middle module bridging via a Med17-Med21 interaction is inferred from yeast conservation, not directly demonstrated in P. falciparum [#0].\",\n      \"The PfSET10 network association derives from TurboID proximity labeling (Low confidence), not direct Co-IP, and carries no MED17-specific functional follow-up [#1].\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0520200","name":null,"product":"mediator of RNA polymerase II transcription subunit 17, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1235000","name":null,"product":"mediator of RNA polymerase II transcription subunit 17, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1012900","name":null,"product":"mediator of RNA polymerase II transcription subunit 17, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"MED17","evaluation":null}