{"gene":"PF3D7_0509100","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2020,"finding":"SMC4 (PF3D7_0509100), together with SMC2, localizes to a distinct centromeric focus during early schizogony (confirmed by co-localization with NDC80 and ChIP-seq analysis), then redistributes diffusely on chromosomes and in the nucleus in mature schizonts and during male gametogenesis. At these later stages, SMC4 forms both condensin I and condensin II complexes. During early schizogony, SMC4/SMC2 do not form condensin I or II complexes, indicating stage-specific complex assembly.","method":"Live-cell fluorescence imaging (GFP-tagged SMC4), ChIP-seq, co-immunoprecipitation to detect condensin I/II complex formation, genetic knockdown","journal":"Cell reports","confidence":"High","confidence_rationale":"Tier 2 / Strong — multiple orthogonal methods (live imaging, ChIP-seq, Co-IP for complex composition, genetic knockdown) in a single focused study on PF3D7_0509100","pmids":["32049018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2020,"finding":"Knockdown of smc4 gene expression (PF3D7_0509100) causes essential defects in parasite proliferation during schizogony and impairs transmission by reducing male gametogenesis (exflagellation), demonstrating that SMC4 is required for both asexual and sexual proliferation.","method":"Conditional knockdown of smc4, parasite growth assay, exflagellation assay","journal":"Cell reports","confidence":"High","confidence_rationale":"Tier 2 / Strong — clean genetic knockdown with specific proliferation and transmission phenotypic readouts, multiple life-cycle stages assessed in a single dedicated study","pmids":["32049018"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2020,"finding":"SMC4-GFP (PF3D7_0509100) co-localizes with NDC80 (outer kinetochore marker) at the centromere during male gametogenesis, as demonstrated by crossing SMC4-GFP and NDC80-mCherry parasite lines and performing live-cell imaging, confirming the centromeric localization of SMC4 at this stage.","method":"Genetic crossing of fluorescently tagged parasite lines (SMC4-GFP × NDC80-mCherry), live-cell fluorescence microscopy","journal":"Journal of cell science","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct co-localization in live parasites using two orthogonal fluorescent markers, single study confirming earlier finding","pmids":["32501284"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"finding":"SMC4 (PF3D7_0509100) co-precipitates with kinase CDPK4 and kinase MAP2 during male gametogenesis in Plasmodium berghei, suggesting that CDPK4 and MAP2 interact with the condensin complex (SMC2/SMC4) and may regulate SMC4 activity and chromosome condensation during endomitosis.","method":"Immunoprecipitation of CDPK4-GFP and MAP2-GFP followed by mass spectrometry","journal":"Life science alliance","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single co-IP/MS experiment, single lab, no reciprocal pull-down or functional validation of the SMC4-kinase interaction specifically","pmids":["40127922"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"PF3D7_0509100 (SMC4), a core condensin subunit, localizes to centromeres during early schizogony without forming canonical condensin I/II complexes, then redistributes broadly on chromosomes and assembles into both condensin I and condensin II complexes in mature schizonts and during male gametogenesis; genetic knockdown establishes that SMC4 is essential for asexual parasite proliferation and sexual transmission, and immunoprecipitation evidence suggests its activity may be regulated by the kinases CDPK4 and MAP2 during endomitosis."},"narrative":{"mechanistic_narrative":"PF3D7_0509100 (SMC4) is a core condensin subunit that governs chromosome organization during the atypical, closed mitoses of Plasmodium proliferation [PMID:32049018]. Together with SMC2, SMC4 first concentrates at a discrete centromeric focus during early schizogony—co-localizing with the outer kinetochore marker NDC80 and confirmed by ChIP-seq—before redistributing diffusely across chromosomes and the nucleus in mature schizonts and during male gametogenesis [PMID:32049018, PMID:32501284]. Complex assembly is stage-specific: at the early centromeric stage SMC4/SMC2 do not assemble into either holocomplex, whereas at later stages SMC4 forms both condensin I and condensin II [PMID:32049018]. Conditional knockdown establishes that SMC4 is essential for asexual proliferation during schizogony and for transmission, with loss impairing male gametogenesis (exflagellation) [PMID:32049018]. In P. berghei male gametogenesis, SMC4 co-precipitates with the kinases CDPK4 and MAP2, linking condensin to endomitotic regulation [PMID:40127922]. Beyond these findings, the biochemical activity and structural basis of SMC4 function have not been characterized in the available corpus.","teleology":[{"year":2020,"claim":"Established that SMC4 is a condensin subunit with a dynamic, stage-specific chromosomal distribution, answering where and when condensin acts across the Plasmodium proliferative cycle.","evidence":"Live-cell imaging of GFP-tagged SMC4, ChIP-seq, and Co-IP for condensin I/II complex composition in P. falciparum and P. berghei","pmids":["32049018"],"confidence":"High","gaps":["What triggers the switch from centromeric focus to diffuse chromosomal distribution is unknown","The molecular signal driving stage-specific condensin I versus II assembly is not identified","No structural or ATPase activity data for SMC4"],"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2020,"claim":"Demonstrated that SMC4 is functionally essential across the life cycle, answering whether condensin activity is dispensable and connecting it to both proliferation and transmission.","evidence":"Conditional knockdown of smc4 with proliferation and exflagellation phenotypic assays in P. falciparum and P. berghei","pmids":["32049018"],"confidence":"High","gaps":["The specific chromosomal defect underlying the proliferation failure is not resolved","Whether the exflagellation defect reflects a condensation failure or another role is unclear"],"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":2020,"claim":"Confirmed SMC4 centromeric localization during male gametogenesis independently, refining the timing of condensin recruitment relative to the kinetochore.","evidence":"Genetic crossing of SMC4-GFP and NDC80-mCherry lines with live-cell imaging in P. berghei","pmids":["32501284"],"confidence":"Medium","gaps":["The mechanism recruiting SMC4 to centromeres is not defined","Whether SMC4 physically contacts kinetochore components is untested"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"claim":"Linked SMC4/condensin to kinase signaling by showing co-precipitation with CDPK4 and MAP2, raising the possibility that condensation is regulated by mitotic kinases during endomitosis.","evidence":"Immunoprecipitation of CDPK4-GFP and MAP2-GFP followed by mass spectrometry in P. berghei","pmids":["40127922"],"confidence":"Low","gaps":["Single co-IP/MS without reciprocal pull-down or functional validation of the SMC4-kinase interaction specifically","No evidence that SMC4 is a direct kinase substrate","Functional consequence of the interaction on condensation is not tested"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The biochemical activity of SMC4, the signal driving stage-specific condensin I/II assembly, and whether kinase regulation acts directly on SMC4 remain unresolved.","evidence":"","pmids":[],"confidence":"Low","gaps":["No structural model or in vitro reconstitution of Plasmodium SMC4-containing condensin","Direct substrate/phosphosite evidence for CDPK4/MAP2 regulation of SMC4 is absent","Mechanism of the centromere-to-chromosome-arm redistribution is uncharacterized"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["condensin I complex","condensin II complex"],"partners":["SMC2","NDC80 (CO-LOCALIZATION AT CENTROMERE)","CDPK4 (CO-PRECIPITATION)","MAP2 (CO-PRECIPITATION)"],"other_free_text":["SMC4/SMC2 do not assemble into condensin I or II during early schizogony despite centromeric co-localization, indicating stage-specific holocomplex assembly.","Essential for asexual proliferation during schizogony and for male gametogenesis/transmission (exflagellation).","Kinase interaction evidence (CDPK4, MAP2) is a single low-confidence co-IP/MS finding in P. berghei and not functionally validated for SMC4 specifically."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"32049018","id":"PMC_32049018","title":"Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission.","date":"2020","source":"Cell reports","url":"https://pubmed.ncbi.nlm.nih.gov/32049018","citation_count":30,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"38411954","id":"","title":"Neddylation is essential for malaria transmission in Plasmodium berghei.","date":"2024","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/38411954","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"35920043","id":"","title":"Plasmodium berghei leucine-rich repeat protein 1 downregulates protein phosphatase 1 activity and is required for efficient oocyst development.","date":"2022","source":"Open biology","url":"https://pubmed.ncbi.nlm.nih.gov/35920043","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40127922","id":"","title":"Divergent Plasmodium kinases drive MTOC, kinetochore and axoneme organisation in male gametogenesis.","date":"2025","source":"Life science alliance","url":"https://pubmed.ncbi.nlm.nih.gov/40127922","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"36040030","id":"","title":"TurboID Identification of Evolutionarily Divergent Components of the Nuclear Pore Complex in the Malaria Model Plasmodium berghei.","date":"2022","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/36040030","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"34835432","id":"","title":"Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini-Chromosome Maintenance Complex Binding Protein.","date":"2021","source":"Microorganisms","url":"https://pubmed.ncbi.nlm.nih.gov/34835432","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"32501284","id":"","title":"Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation.","date":"2020","source":"Journal of cell science","url":"https://pubmed.ncbi.nlm.nih.gov/32501284","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"33912479","id":"","title":"The Modular Circuitry of Apicomplexan Cell Division Plasticity.","date":"2021","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/33912479","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["SMC4"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":10387,"output_tokens":1301,"usd":0.025338,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6191,"output_tokens":2386,"usd":0.045303,"stage2_stop_reason":"end_turn"},"total_usd":0.070641,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2020,\n      \"finding\": \"SMC4 (PF3D7_0509100), together with SMC2, localizes to a distinct centromeric focus during early schizogony (confirmed by co-localization with NDC80 and ChIP-seq analysis), then redistributes diffusely on chromosomes and in the nucleus in mature schizonts and during male gametogenesis. At these later stages, SMC4 forms both condensin I and condensin II complexes. During early schizogony, SMC4/SMC2 do not form condensin I or II complexes, indicating stage-specific complex assembly.\",\n      \"method\": \"Live-cell fluorescence imaging (GFP-tagged SMC4), ChIP-seq, co-immunoprecipitation to detect condensin I/II complex formation, genetic knockdown\",\n      \"journal\": \"Cell reports\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — multiple orthogonal methods (live imaging, ChIP-seq, Co-IP for complex composition, genetic knockdown) in a single focused study on PF3D7_0509100\",\n      \"pmids\": [\"32049018\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"Knockdown of smc4 gene expression (PF3D7_0509100) causes essential defects in parasite proliferation during schizogony and impairs transmission by reducing male gametogenesis (exflagellation), demonstrating that SMC4 is required for both asexual and sexual proliferation.\",\n      \"method\": \"Conditional knockdown of smc4, parasite growth assay, exflagellation assay\",\n      \"journal\": \"Cell reports\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — clean genetic knockdown with specific proliferation and transmission phenotypic readouts, multiple life-cycle stages assessed in a single dedicated study\",\n      \"pmids\": [\"32049018\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"SMC4-GFP (PF3D7_0509100) co-localizes with NDC80 (outer kinetochore marker) at the centromere during male gametogenesis, as demonstrated by crossing SMC4-GFP and NDC80-mCherry parasite lines and performing live-cell imaging, confirming the centromeric localization of SMC4 at this stage.\",\n      \"method\": \"Genetic crossing of fluorescently tagged parasite lines (SMC4-GFP × NDC80-mCherry), live-cell fluorescence microscopy\",\n      \"journal\": \"Journal of cell science\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct co-localization in live parasites using two orthogonal fluorescent markers, single study confirming earlier finding\",\n      \"pmids\": [\"32501284\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"SMC4 (PF3D7_0509100) co-precipitates with kinase CDPK4 and kinase MAP2 during male gametogenesis in Plasmodium berghei, suggesting that CDPK4 and MAP2 interact with the condensin complex (SMC2/SMC4) and may regulate SMC4 activity and chromosome condensation during endomitosis.\",\n      \"method\": \"Immunoprecipitation of CDPK4-GFP and MAP2-GFP followed by mass spectrometry\",\n      \"journal\": \"Life science alliance\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single co-IP/MS experiment, single lab, no reciprocal pull-down or functional validation of the SMC4-kinase interaction specifically\",\n      \"pmids\": [\"40127922\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_0509100 (SMC4), a core condensin subunit, localizes to centromeres during early schizogony without forming canonical condensin I/II complexes, then redistributes broadly on chromosomes and assembles into both condensin I and condensin II complexes in mature schizonts and during male gametogenesis; genetic knockdown establishes that SMC4 is essential for asexual parasite proliferation and sexual transmission, and immunoprecipitation evidence suggests its activity may be regulated by the kinases CDPK4 and MAP2 during endomitosis.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0509100 (SMC4) is a core condensin subunit that governs chromosome organization during the atypical, closed mitoses of Plasmodium proliferation [#0]. Together with SMC2, SMC4 first concentrates at a discrete centromeric focus during early schizogony\\u2014co-localizing with the outer kinetochore marker NDC80 and confirmed by ChIP-seq\\u2014before redistributing diffusely across chromosomes and the nucleus in mature schizonts and during male gametogenesis [#0, #2]. Complex assembly is stage-specific: at the early centromeric stage SMC4/SMC2 do not assemble into either holocomplex, whereas at later stages SMC4 forms both condensin I and condensin II [#0]. Conditional knockdown establishes that SMC4 is essential for asexual proliferation during schizogony and for transmission, with loss impairing male gametogenesis (exflagellation) [#1]. In P. berghei male gametogenesis, SMC4 co-precipitates with the kinases CDPK4 and MAP2, linking condensin to endomitotic regulation [#3]. Beyond these findings, the biochemical activity and structural basis of SMC4 function have not been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2020,\n      \"claim\": \"Established that SMC4 is a condensin subunit with a dynamic, stage-specific chromosomal distribution, answering where and when condensin acts across the Plasmodium proliferative cycle.\",\n      \"evidence\": \"Live-cell imaging of GFP-tagged SMC4, ChIP-seq, and Co-IP for condensin I/II complex composition in P. falciparum and P. berghei\",\n      \"pmids\": [\"32049018\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"What triggers the switch from centromeric focus to diffuse chromosomal distribution is unknown\", \"The molecular signal driving stage-specific condensin I versus II assembly is not identified\", \"No structural or ATPase activity data for SMC4\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}, {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2020,\n      \"claim\": \"Demonstrated that SMC4 is functionally essential across the life cycle, answering whether condensin activity is dispensable and connecting it to both proliferation and transmission.\",\n      \"evidence\": \"Conditional knockdown of smc4 with proliferation and exflagellation phenotypic assays in P. falciparum and P. berghei\",\n      \"pmids\": [\"32049018\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"The specific chromosomal defect underlying the proliferation failure is not resolved\", \"Whether the exflagellation defect reflects a condensation failure or another role is unclear\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}, {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2020,\n      \"claim\": \"Confirmed SMC4 centromeric localization during male gametogenesis independently, refining the timing of condensin recruitment relative to the kinetochore.\",\n      \"evidence\": \"Genetic crossing of SMC4-GFP and NDC80-mCherry lines with live-cell imaging in P. berghei\",\n      \"pmids\": [\"32501284\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"The mechanism recruiting SMC4 to centromeres is not defined\", \"Whether SMC4 physically contacts kinetochore components is untested\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Linked SMC4/condensin to kinase signaling by showing co-precipitation with CDPK4 and MAP2, raising the possibility that condensation is regulated by mitotic kinases during endomitosis.\",\n      \"evidence\": \"Immunoprecipitation of CDPK4-GFP and MAP2-GFP followed by mass spectrometry in P. berghei\",\n      \"pmids\": [\"40127922\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"Single co-IP/MS without reciprocal pull-down or functional validation of the SMC4-kinase interaction specifically\", \"No evidence that SMC4 is a direct kinase substrate\", \"Functional consequence of the interaction on condensation is not tested\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The biochemical activity of SMC4, the signal driving stage-specific condensin I/II assembly, and whether kinase regulation acts directly on SMC4 remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No structural model or in vitro reconstitution of Plasmodium SMC4-containing condensin\", \"Direct substrate/phosphosite evidence for CDPK4/MAP2 regulation of SMC4 is absent\", \"Mechanism of the centromere-to-chromosome-arm redistribution is uncharacterized\"],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003682\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0000775\", \"supporting_discovery_ids\": [0, 2]},\n      {\"term_id\": \"GO:0005694\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0030261\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0007140\", \"supporting_discovery_ids\": [1, 3]},\n      {\"term_id\": \"GO:0007283\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"complexes\": [\n      \"condensin I complex\",\n      \"condensin II complex\"\n    ],\n    \"partners\": [\n      \"SMC2\",\n      \"NDC80 (co-localization at centromere)\",\n      \"CDPK4 (co-precipitation)\",\n      \"MAP2 (co-precipitation)\"\n    ],\n    \"other_free_text\": [\n      \"SMC4/SMC2 do not assemble into condensin I or II during early schizogony despite centromeric co-localization, indicating stage-specific holocomplex assembly.\",\n      \"Essential for asexual proliferation during schizogony and for male gametogenesis/transmission (exflagellation).\",\n      \"Kinase interaction evidence (CDPK4, MAP2) is a single low-confidence co-IP/MS finding in P. berghei and not functionally validated for SMC4 specifically.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0509100","name":null,"product":"structural maintenance of chromosomes protein 4, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1108700","name":null,"product":"structural maintenance of chromosomes protein 4","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_1024500","name":null,"product":"structural maintenance of chromosomes protein 4, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"SMC4","evaluation":null}