{"gene":"PF3D7_0420600","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2022,"finding":"PF3D7_0420600 (AKIT6) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, establishing its membership in the apicomplexan kinetochore complex.","method":"Interactomics (co-immunoprecipitation/mass spectrometry) combined with deep homology searches; subkinetochore localization mapping","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — interactomics-based identification in a single study; no independent replication and no functional follow-up specific to AKIT6 reported in the abstract","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"AKIT6 (PF3D7_0420600) is a component of the apicomplexan kinetochore complex, identified by interactomics and deep homology searches, but no further mechanistic details about its specific function, binding partners, or catalytic activity have been experimentally established."},"narrative":{"mechanistic_narrative":"AKIT6 (PF3D7_0420600) is a component of the apicomplexan kinetochore complex in Plasmodium falciparum, identified as one of 13 previously unknown kinetochore components through interactomics combined with deep homology searches, with subkinetochore localization mapping placing it within the kinetochore [PMID:36006241]. Beyond this membership assignment [PMID:36006241], no further mechanistic detail about AKIT6's specific function, binding partners, or catalytic activity has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"Before this work the apicomplexan kinetochore was known to diverge from canonical eukaryotic kinetochores, leaving many of its components unidentified; combining interactomics with deep homology searches assigned AKIT6 to the kinetochore, establishing that PF3D7_0420600 is a bona fide constituent of this complex.","evidence":"Co-immunoprecipitation/mass spectrometry with deep homology searches and subkinetochore localization mapping in P. falciparum","pmids":["36006241"],"confidence":"Medium","gaps":["No functional follow-up specific to AKIT6 (loss-of-function, essentiality, or role in chromosome segregation) reported","Direct binding partners within the kinetochore not resolved for AKIT6 individually","No independent replication of the identification"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"What AKIT6 does molecularly within the kinetochore — its direct interaction partners, any catalytic or structural role, and its contribution to mitotic chromosome segregation — remains unestablished.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No substrate or catalytic activity characterized","No structural model or defined subcomplex assignment beyond kinetochore membership","No phenotype from genetic perturbation of AKIT6"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["apicomplexan kinetochore complex"],"partners":[],"other_free_text":["Identified as one of 13 previously unknown apicomplexan kinetochore components via interactomics and deep homology searches in P. falciparum [#0]","Subkinetochore localization mapped, placing AKIT6 within the kinetochore [#0]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":31,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3258,"output_tokens":345,"usd":0.007475,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4808,"output_tokens":996,"usd":0.02447,"stage2_stop_reason":"end_turn"},"total_usd":0.031945,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"PF3D7_0420600 (AKIT6) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, establishing its membership in the apicomplexan kinetochore complex.\",\n      \"method\": \"Interactomics (co-immunoprecipitation/mass spectrometry) combined with deep homology searches; subkinetochore localization mapping\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — interactomics-based identification in a single study; no independent replication and no functional follow-up specific to AKIT6 reported in the abstract\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"AKIT6 (PF3D7_0420600) is a component of the apicomplexan kinetochore complex, identified by interactomics and deep homology searches, but no further mechanistic details about its specific function, binding partners, or catalytic activity have been experimentally established.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AKIT6 (PF3D7_0420600) is a component of the apicomplexan kinetochore complex in Plasmodium falciparum, identified as one of 13 previously unknown kinetochore components through interactomics combined with deep homology searches, with subkinetochore localization mapping placing it within the kinetochore [#0]. Beyond this membership assignment [#0], no further mechanistic detail about AKIT6's specific function, binding partners, or catalytic activity has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Before this work the apicomplexan kinetochore was known to diverge from canonical eukaryotic kinetochores, leaving many of its components unidentified; combining interactomics with deep homology searches assigned AKIT6 to the kinetochore, establishing that PF3D7_0420600 is a bona fide constituent of this complex.\",\n      \"evidence\": \"Co-immunoprecipitation/mass spectrometry with deep homology searches and subkinetochore localization mapping in P. falciparum\",\n      \"pmids\": [\"36006241\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional follow-up specific to AKIT6 (loss-of-function, essentiality, or role in chromosome segregation) reported\",\n        \"Direct binding partners within the kinetochore not resolved for AKIT6 individually\",\n        \"No independent replication of the identification\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"What AKIT6 does molecularly within the kinetochore — its direct interaction partners, any catalytic or structural role, and its contribution to mitotic chromosome segregation — remains unestablished.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No substrate or catalytic activity characterized\",\n        \"No structural model or defined subcomplex assignment beyond kinetochore membership\",\n        \"No phenotype from genetic perturbation of AKIT6\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0000776\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"apicomplexan kinetochore complex\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as one of 13 previously unknown apicomplexan kinetochore components via interactomics and deep homology searches in P. falciparum [#0]\",\n      \"Subkinetochore localization mapped, placing AKIT6 within the kinetochore [#0]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0420600","name":null,"product":"apicomplexan kinetochore protein 6, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0522000","name":null,"product":"conserved Plasmodium protein, unknown function","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0513400","name":null,"product":"apicomplexan kinetochore protein 6, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AKIT6","evaluation":null}