{"gene":"PF3D7_0418200","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2024,"finding":"EIF3M (PF3D7_0418200) was identified as a component of stress granule-like mRNPs in nutritionally stressed P. falciparum schizonts, co-purifying with PfDOZI, PABP2, CITH, PAIP1, and the eIF2B complex, but absent from gametocyte PfDOZI-associated granules, indicating stage-specific incorporation into distinct mRNP complexes.","method":"Co-immunoprecipitation / quantitative interactome proteomics of PfDOZI under stress conditions","journal":"Nature communications","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — single-lab Co-IP/MS interactome; EIF3M detected as a component but not individually validated with reciprocal pull-down or mutagenesis","pmids":["38702310"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2020,"finding":"EIF3M (PF3D7_0418200) undergoes phosphorylation and 2-hydroxyisobutyrylation as post-translational modifications in P. falciparum blood-stage parasites, detected in a proteome-wide PTM profiling study.","method":"Mass spectrometry-based proteome-wide PTM mapping across intraerythrocytic developmental stages","journal":"Molecular & cellular proteomics : MCP","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single large-scale proteomics screen; no writers/erasers/readers identified for EIF3M specifically, no functional validation of the modifications","pmids":["33517144"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"current_model":"PF3D7_0418200 (EIF3M), the putative eukaryotic translation initiation factor 3 subunit M of P. falciparum, associates with stress granule-like mRNP complexes (containing PfDOZI, PABP2, CITH, PAIP1, and eIF2B) specifically in nutritionally stressed schizonts, and undergoes phosphorylation and 2-hydroxyisobutyrylation during blood-stage development, though no writers, erasers, or functional consequences of these modifications have been established beyond complex membership."},"narrative":{"mechanistic_narrative":"PF3D7_0418200 (EIF3M), a putative eukaryotic translation initiation factor 3 subunit of Plasmodium falciparum, is defined in the available corpus principally by its association with translationally repressed mRNP complexes during blood-stage development [PMID:38702310]. It was identified as a component of stress granule-like mRNPs that assemble in nutritionally stressed schizonts, co-purifying with PfDOZI, PABP2, CITH, PAIP1, and the eIF2B complex; notably, it is absent from the PfDOZI-associated granules of gametocytes, indicating stage-specific incorporation into distinct mRNP assemblies [PMID:38702310]. EIF3M also carries phosphorylation and 2-hydroxyisobutyrylation marks detected in blood-stage parasites [PMID:33517144]. Beyond this complex membership and the presence of these post-translational modifications, no writers, erasers, functional consequences, or independent biochemical activity of PF3D7_0418200 has been characterized in the available corpus.","teleology":[{"year":2020,"claim":"Establishing that PF3D7_0418200 is a modified protein during the intraerythrocytic cycle raised the question of whether its function is regulated post-translationally, providing the first molecular handle on the protein.","evidence":"Mass spectrometry-based proteome-wide PTM mapping across intraerythrocytic developmental stages in P. falciparum","pmids":["33517144"],"confidence":"Low","gaps":["No writers, erasers, or readers of the phosphorylation or 2-hydroxyisobutyrylation marks were identified for EIF3M","No functional consequence of the modifications was tested","Single large-scale proteomics screen without targeted validation"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"claim":"Placing PF3D7_0418200 within stress granule-like mRNPs answered where the protein acts, linking it to translational repression machinery under nutritional stress and revealing stage-specific complex composition.","evidence":"Co-immunoprecipitation / quantitative interactome proteomics of PfDOZI under stress conditions in P. falciparum schizonts and gametocytes","pmids":["38702310"],"confidence":"Medium","gaps":["EIF3M was detected as a component but not individually validated by reciprocal pull-down or mutagenesis","Whether EIF3M is required for mRNP assembly, stability, or translational repression is unknown","The molecular basis for its exclusion from gametocyte PfDOZI granules is not resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":null,"claim":"Whether PF3D7_0418200 performs a canonical eIF3-type translation initiation function, or acts primarily as a stress-responsive mRNP component, remains unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No direct assay of translation initiation activity for EIF3M","No genetic knockout or knockdown phenotype reported","No structural or reciprocal biochemical validation of its complex associations"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Stress granule-like mRNP containing PfDOZI, PABP2, CITH, PAIP1, and eIF2B (nutritionally stressed schizonts) [#0]"],"partners":["PFDOZI [#0]","PABP2 [#0]","CITH [#0]","PAIP1 [#0]","EIF2B COMPLEX [#0]"],"other_free_text":["Undergoes phosphorylation and 2-hydroxyisobutyrylation in blood-stage parasites; no writers, erasers, or functional consequences established [#1]","Stage-specific mRNP incorporation: present in stressed schizont mRNPs but absent from gametocyte PfDOZI granules [#0]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"24681218","id":"PMC_24681218","title":"Differential T-cell responses of semi-immune and susceptible malaria subjects to in silico predicted and synthetic peptides of Plasmodium falciparum.","date":"2014","source":"Acta tropica","url":"https://pubmed.ncbi.nlm.nih.gov/24681218","citation_count":5,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["PFD0880w"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40491475","id":"","title":"Covalent inhibition of Plasmodium falciparum Ubc13 impairs global protein synthesis.","date":"2025","source":"iScience","url":"https://pubmed.ncbi.nlm.nih.gov/40491475","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["EIF3M"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"38702310","id":"","title":"The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum.","date":"2024","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/38702310","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["EIF3M"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"33517144","id":"","title":"Protein Modification Characteristics of the Malaria Parasite Plasmodium falciparum and the Infected Erythrocytes.","date":"2020","source":"Molecular & cellular proteomics : MCP","url":"https://pubmed.ncbi.nlm.nih.gov/33517144","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["EIF3M"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":6788,"output_tokens":694,"usd":0.015387,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5293,"output_tokens":1592,"usd":0.033133,"stage2_stop_reason":"end_turn"},"total_usd":0.04852,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2024,\n      \"finding\": \"EIF3M (PF3D7_0418200) was identified as a component of stress granule-like mRNPs in nutritionally stressed P. falciparum schizonts, co-purifying with PfDOZI, PABP2, CITH, PAIP1, and the eIF2B complex, but absent from gametocyte PfDOZI-associated granules, indicating stage-specific incorporation into distinct mRNP complexes.\",\n      \"method\": \"Co-immunoprecipitation / quantitative interactome proteomics of PfDOZI under stress conditions\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — single-lab Co-IP/MS interactome; EIF3M detected as a component but not individually validated with reciprocal pull-down or mutagenesis\",\n      \"pmids\": [\"38702310\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"EIF3M (PF3D7_0418200) undergoes phosphorylation and 2-hydroxyisobutyrylation as post-translational modifications in P. falciparum blood-stage parasites, detected in a proteome-wide PTM profiling study.\",\n      \"method\": \"Mass spectrometry-based proteome-wide PTM mapping across intraerythrocytic developmental stages\",\n      \"journal\": \"Molecular & cellular proteomics : MCP\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single large-scale proteomics screen; no writers/erasers/readers identified for EIF3M specifically, no functional validation of the modifications\",\n      \"pmids\": [\"33517144\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PF3D7_0418200 (EIF3M), the putative eukaryotic translation initiation factor 3 subunit M of P. falciparum, associates with stress granule-like mRNP complexes (containing PfDOZI, PABP2, CITH, PAIP1, and eIF2B) specifically in nutritionally stressed schizonts, and undergoes phosphorylation and 2-hydroxyisobutyrylation during blood-stage development, though no writers, erasers, or functional consequences of these modifications have been established beyond complex membership.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PF3D7_0418200 (EIF3M), a putative eukaryotic translation initiation factor 3 subunit of Plasmodium falciparum, is defined in the available corpus principally by its association with translationally repressed mRNP complexes during blood-stage development [#0]. It was identified as a component of stress granule-like mRNPs that assemble in nutritionally stressed schizonts, co-purifying with PfDOZI, PABP2, CITH, PAIP1, and the eIF2B complex; notably, it is absent from the PfDOZI-associated granules of gametocytes, indicating stage-specific incorporation into distinct mRNP assemblies [#0]. EIF3M also carries phosphorylation and 2-hydroxyisobutyrylation marks detected in blood-stage parasites [#1]. Beyond this complex membership and the presence of these post-translational modifications, no writers, erasers, functional consequences, or independent biochemical activity of PF3D7_0418200 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2020,\n      \"claim\": \"Establishing that PF3D7_0418200 is a modified protein during the intraerythrocytic cycle raised the question of whether its function is regulated post-translationally, providing the first molecular handle on the protein.\",\n      \"evidence\": \"Mass spectrometry-based proteome-wide PTM mapping across intraerythrocytic developmental stages in P. falciparum\",\n      \"pmids\": [\n        \"33517144\"\n      ],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No writers, erasers, or readers of the phosphorylation or 2-hydroxyisobutyrylation marks were identified for EIF3M\",\n        \"No functional consequence of the modifications was tested\",\n        \"Single large-scale proteomics screen without targeted validation\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Placing PF3D7_0418200 within stress granule-like mRNPs answered where the protein acts, linking it to translational repression machinery under nutritional stress and revealing stage-specific complex composition.\",\n      \"evidence\": \"Co-immunoprecipitation / quantitative interactome proteomics of PfDOZI under stress conditions in P. falciparum schizonts and gametocytes\",\n      \"pmids\": [\n        \"38702310\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"EIF3M was detected as a component but not individually validated by reciprocal pull-down or mutagenesis\",\n        \"Whether EIF3M is required for mRNP assembly, stability, or translational repression is unknown\",\n        \"The molecular basis for its exclusion from gametocyte PfDOZI granules is not resolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether PF3D7_0418200 performs a canonical eIF3-type translation initiation function, or acts primarily as a stress-responsive mRNP component, remains unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct assay of translation initiation activity for EIF3M\",\n        \"No genetic knockout or knockdown phenotype reported\",\n        \"No structural or reciprocal biochemical validation of its complex associations\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Plasmodium falciparum\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Stress granule-like mRNP containing PfDOZI, PABP2, CITH, PAIP1, and eIF2B (nutritionally stressed schizonts) [#0]\"\n    ],\n    \"partners\": [\n      \"PfDOZI [#0]\",\n      \"PABP2 [#0]\",\n      \"CITH [#0]\",\n      \"PAIP1 [#0]\",\n      \"eIF2B complex [#0]\"\n    ],\n    \"other_free_text\": [\n      \"Undergoes phosphorylation and 2-hydroxyisobutyrylation in blood-stage parasites; no writers, erasers, or functional consequences established [#1]\",\n      \"Stage-specific mRNP incorporation: present in stressed schizont mRNPs but absent from gametocyte PfDOZI granules [#0]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0418200","name":null,"product":"eukaryotic translation initiation factor 3 subunit M, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0720300","name":null,"product":"eukaryotic translation initiation factor 3 subunit M, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0510600","name":null,"product":"eukaryotic translation initiation factor 3 subunit M, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"EIF3M","evaluation":null}