{"gene":"PF3D7_0410900","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2022,"finding":"AKIT7 (PF3D7_0410900) was identified as a previously unknown kinetochore component in apicomplexan parasites through interactomics combined with deep homology searches. It localizes to kinetochores and is part of a multiprotein kinetochore assembly with at least four discrete compartments displaying different biochemical interactions and subkinetochore localizations.","method":"Interactomics (immunoprecipitation/mass spectrometry) and deep homology searches; localization by microscopy","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — interactomics with localization data in a single study; multiple orthogonal methods (IP-MS + imaging) but single lab","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""},{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"finding":"AKIT7 (PF3D7_0410900 / PBANKA_0612300 ortholog) was identified as an interaction partner of the Arp2/3 subunit ARPC1 by co-immunoprecipitation of ARPC1-GFP from activated (but not non-activated) male gametocytes, placing AKIT7 in physical association with the atypical Plasmodium Arp2/3 complex specifically during gametogenesis.","method":"Co-immunoprecipitation of ARPC1-GFP followed by mass spectrometry from activated gametocytes; negative result in non-activated gametocytes","journal":"Nature microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal-style IP-MS with appropriate GFP control, single lab, activation-dependent specificity demonstrated","pmids":["40514561"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"finding":"In the absence of ARPC1, kinetochores partially detached from spindles and resided freely in the nucleus during male gametogenesis, and AKIT7 (associated with the Arp2/3 complex) is implicated at the kinetochore-spindle interface, suggesting AKIT7 contributes to kinetochore-spindle attachment. The function of AKIT7 within this complex had not been previously investigated.","method":"Genetic disruption of ARPC1 combined with kinetochore localization imaging; AlphaFold structural prediction of AKIT7","journal":"Cytoskeleton (Hoboken, N.J.)","confidence":"Low","confidence_rationale":"Tier 3 / Weak — functional inference from ARPC1 KO phenotype attributed partly to AKIT7 association; no direct manipulation of AKIT7 itself; single lab, single study","pmids":["40828051"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2026,"finding":"AKIT7 (PF3D7_0410900 ortholog) was identified in the ARK1 interactome from male-only microgametocytes under high cross-linking conditions, annotated as a spindle assembly checkpoint protein homolog (MAD1). It was also found to associate with SKA/PP1 and AKiT7-associated modules preferentially during meiosis rather than male mitosis, suggesting stage-specific engagement.","method":"Cross-linking immunoprecipitation (native and cross-linked conditions) of ARK1-3xHA followed by mass spectrometry; comparison across meiotic and mitotic stages","journal":"Nature communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — detected only under high cross-linking (labile/indirect interaction), single lab, no direct functional manipulation of AKIT7","pmids":["42323312"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2023,"finding":"AKIT7 (annotated as Mad1/AKiT7) co-varied with ARK2 and EB1 in principal component analysis of co-immunoprecipitation mass spectrometry data from gametocytes, consistent with its presence in the same kinetochore/spindle-associated cellular structures as ARK2 and EB1.","method":"Co-immunoprecipitation (EB1-GFP and ARK2-GFP) followed by mass spectrometry and PCA of peptide spectral counts","journal":"Nature communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — co-variation in PCA of pulldown data; indirect association, no direct manipulation of AKIT7, single lab","pmids":["37704606"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"AKIT7 (PF3D7_0410900) is an apicomplexan-specific kinetochore protein that physically associates with the atypical Plasmodium Arp2/3 complex (via ARPC1) specifically during activated male gametogenesis, localizes to kinetochores within a multi-compartment kinetochore assembly, and is implicated—based on ARPC1 loss-of-function phenotypes—in kinetochore-spindle attachment during DNA segregation; it also co-associates with the Aurora-related kinase ARK1 under cross-linking conditions and co-varies with ARK2/EB1 spindle complexes, and has been annotated as a MAD1 (spindle assembly checkpoint) homolog, though no direct loss-of-function experiment targeting AKIT7 itself has yet been reported."},"narrative":{"mechanistic_narrative":"AKIT7 (PF3D7_0410900) is an apicomplexan-specific kinetochore protein identified through interactomics and deep homology searches, localizing to kinetochores as part of a multiprotein kinetochore assembly organized into at least four biochemically and spatially distinct compartments [PMID:36006241]. During activated male gametogenesis in P. berghei, AKIT7 physically associates with the atypical Plasmodium Arp2/3 complex, being recovered in ARPC1-GFP co-immunoprecipitations specifically from activated but not non-activated gametocytes [PMID:40514561]. This activation-dependent association places AKIT7 at the kinetochore-spindle interface: loss of ARPC1 causes kinetochores to partially detach from spindles and reside freely in the nucleus, implicating the AKIT7-associated Arp2/3 complex in kinetochore-spindle attachment during chromosome segregation, although AKIT7 itself has not been directly manipulated [PMID:40828051]. No direct loss-of-function experiment targeting AKIT7 has been reported in the available corpus; its role is inferred from complex membership and the phenotypes of interacting subunits.","teleology":[{"year":2022,"claim":"Establishing that AKIT7 is a bona fide kinetochore component answered whether this uncharacterized apicomplexan protein has a defined subcellular assignment, placing it within the parasite's divergent kinetochore machinery.","evidence":"Interactomics (IP-MS) plus deep homology searches with microscopy-based localization","pmids":["36006241"],"confidence":"Medium","gaps":["No direct functional manipulation of AKIT7 to test its requirement for kinetochore assembly","Which of the four kinetochore compartments AKIT7 occupies and its direct binding partners within them are not resolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""},{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"claim":"Co-variation of AKIT7 with ARK2 and EB1 in pulldown data addressed which spindle-associated structures AKIT7 belongs to, linking it to the ARK2/EB1 kinetochore-spindle module during gametocyte development.","evidence":"EB1-GFP and ARK2-GFP co-IP mass spectrometry with PCA of spectral counts in gametocytes","pmids":["37704606"],"confidence":"Low","gaps":["Co-variation in PCA is an indirect association, not a demonstrated physical interaction","No direct manipulation of AKIT7 to test functional relevance of the ARK2/EB1 association"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"claim":"Identifying AKIT7 as an ARPC1 interactor and observing kinetochore detachment upon ARPC1 loss addressed how kinetochores couple to spindles, implicating the AKIT7-associated Arp2/3 complex in this attachment specifically during activated male gametogenesis.","evidence":"ARPC1-GFP co-IP/MS with activation-dependent controls (P. berghei); ARPC1 genetic disruption with kinetochore imaging and AlphaFold prediction","pmids":["40514561","40828051"],"confidence":"Low","gaps":["Kinetochore-attachment function is inferred from the ARPC1 knockout phenotype, not from disrupting AKIT7 itself","Whether the AKIT7-ARPC1 association is direct or bridged by other subunits is unknown","The molecular basis of AKIT7's activation-dependent recruitment is not defined"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2026,"claim":"Detection of AKIT7 in the ARK1 interactome under cross-linking and its stage-biased association with SKA/PP1 modules addressed when AKIT7 engages checkpoint/kinase machinery, pointing to preferential engagement during meiosis versus male mitosis.","evidence":"Native and cross-linked ARK1-3xHA IP-MS compared across meiotic and mitotic stages (P. berghei)","pmids":["42323312"],"confidence":"Low","gaps":["Interaction detected only under high cross-linking, indicating a labile or indirect association","The MAD1/spindle-assembly-checkpoint homology annotation is not backed by functional checkpoint assays on AKIT7","No direct functional manipulation of AKIT7 in meiosis versus mitosis"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"Whether AKIT7 is itself required for kinetochore assembly, kinetochore-spindle attachment, or spindle checkpoint function remains unresolved, as all functional inferences to date rest on partner phenotypes rather than direct AKIT7 loss-of-function.","evidence":"No direct genetic disruption or depletion of AKIT7 reported in the corpus","pmids":[],"confidence":"Low","gaps":["No conditional knockout or knockdown of AKIT7 itself","No structural or biochemical demonstration of a direct AKIT7 binding partner","MAD1/checkpoint activity attributed to AKIT7 by homology remains functionally untested"],"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Apicomplexan multi-compartment kinetochore assembly","Atypical Plasmodium Arp2/3 complex (via ARPC1, activation-dependent)"],"partners":["ARPC1 (ARP2/3 SUBUNIT)","ARK1 (CROSS-LINKING CONDITIONS)","ARK2","EB1","SKA/PP1 MODULES"],"other_free_text":["Annotated as a MAD1 (spindle assembly checkpoint) homolog by homology only; no direct checkpoint function demonstrated for AKIT7 [#3]","No direct loss-of-function experiment targeting AKIT7 itself has been reported in the corpus; functional roles are inferred from ARPC1 knockout phenotypes and complex membership [#2]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":31,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40514561","id":"","title":"An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission.","date":"2025","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/40514561","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"42323312","id":"","title":"A modular chromosomal passenger complex rewires chromosome segregation in Plasmodium berghei.","date":"2026","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/42323312","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41218080","id":"","title":"Plasmodium actin-like proteins are essential for DNA segregation during male gametogenesis and malaria transmission.","date":"2025","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/41218080","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40828051","id":"","title":"Discovery of an Atypical Arp2/3 Complex in Malaria Parasites Sheds New Light on Nuclear Actin.","date":"2025","source":"Cytoskeleton (Hoboken, N.J.)","url":"https://pubmed.ncbi.nlm.nih.gov/40828051","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"37704606","id":"","title":"Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages.","date":"2023","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/37704606","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT7"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":8681,"output_tokens":1655,"usd":0.025434,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6662,"output_tokens":2562,"usd":0.04868,"stage2_stop_reason":"end_turn"},"total_usd":0.074114,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"AKIT7 (PF3D7_0410900) was identified as a previously unknown kinetochore component in apicomplexan parasites through interactomics combined with deep homology searches. It localizes to kinetochores and is part of a multiprotein kinetochore assembly with at least four discrete compartments displaying different biochemical interactions and subkinetochore localizations.\",\n      \"method\": \"Interactomics (immunoprecipitation/mass spectrometry) and deep homology searches; localization by microscopy\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — interactomics with localization data in a single study; multiple orthogonal methods (IP-MS + imaging) but single lab\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"},\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"AKIT7 (PF3D7_0410900 / PBANKA_0612300 ortholog) was identified as an interaction partner of the Arp2/3 subunit ARPC1 by co-immunoprecipitation of ARPC1-GFP from activated (but not non-activated) male gametocytes, placing AKIT7 in physical association with the atypical Plasmodium Arp2/3 complex specifically during gametogenesis.\",\n      \"method\": \"Co-immunoprecipitation of ARPC1-GFP followed by mass spectrometry from activated gametocytes; negative result in non-activated gametocytes\",\n      \"journal\": \"Nature microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal-style IP-MS with appropriate GFP control, single lab, activation-dependent specificity demonstrated\",\n      \"pmids\": [\"40514561\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"In the absence of ARPC1, kinetochores partially detached from spindles and resided freely in the nucleus during male gametogenesis, and AKIT7 (associated with the Arp2/3 complex) is implicated at the kinetochore-spindle interface, suggesting AKIT7 contributes to kinetochore-spindle attachment. The function of AKIT7 within this complex had not been previously investigated.\",\n      \"method\": \"Genetic disruption of ARPC1 combined with kinetochore localization imaging; AlphaFold structural prediction of AKIT7\",\n      \"journal\": \"Cytoskeleton (Hoboken, N.J.)\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — functional inference from ARPC1 KO phenotype attributed partly to AKIT7 association; no direct manipulation of AKIT7 itself; single lab, single study\",\n      \"pmids\": [\"40828051\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"AKIT7 (PF3D7_0410900 ortholog) was identified in the ARK1 interactome from male-only microgametocytes under high cross-linking conditions, annotated as a spindle assembly checkpoint protein homolog (MAD1). It was also found to associate with SKA/PP1 and AKiT7-associated modules preferentially during meiosis rather than male mitosis, suggesting stage-specific engagement.\",\n      \"method\": \"Cross-linking immunoprecipitation (native and cross-linked conditions) of ARK1-3xHA followed by mass spectrometry; comparison across meiotic and mitotic stages\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — detected only under high cross-linking (labile/indirect interaction), single lab, no direct functional manipulation of AKIT7\",\n      \"pmids\": [\"42323312\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"AKIT7 (annotated as Mad1/AKiT7) co-varied with ARK2 and EB1 in principal component analysis of co-immunoprecipitation mass spectrometry data from gametocytes, consistent with its presence in the same kinetochore/spindle-associated cellular structures as ARK2 and EB1.\",\n      \"method\": \"Co-immunoprecipitation (EB1-GFP and ARK2-GFP) followed by mass spectrometry and PCA of peptide spectral counts\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — co-variation in PCA of pulldown data; indirect association, no direct manipulation of AKIT7, single lab\",\n      \"pmids\": [\"37704606\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"AKIT7 (PF3D7_0410900) is an apicomplexan-specific kinetochore protein that physically associates with the atypical Plasmodium Arp2/3 complex (via ARPC1) specifically during activated male gametogenesis, localizes to kinetochores within a multi-compartment kinetochore assembly, and is implicated—based on ARPC1 loss-of-function phenotypes—in kinetochore-spindle attachment during DNA segregation; it also co-associates with the Aurora-related kinase ARK1 under cross-linking conditions and co-varies with ARK2/EB1 spindle complexes, and has been annotated as a MAD1 (spindle assembly checkpoint) homolog, though no direct loss-of-function experiment targeting AKIT7 itself has yet been reported.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AKIT7 (PF3D7_0410900) is an apicomplexan-specific kinetochore protein identified through interactomics and deep homology searches, localizing to kinetochores as part of a multiprotein kinetochore assembly organized into at least four biochemically and spatially distinct compartments [#0]. During activated male gametogenesis in P. berghei, AKIT7 physically associates with the atypical Plasmodium Arp2/3 complex, being recovered in ARPC1-GFP co-immunoprecipitations specifically from activated but not non-activated gametocytes [#1]. This activation-dependent association places AKIT7 at the kinetochore-spindle interface: loss of ARPC1 causes kinetochores to partially detach from spindles and reside freely in the nucleus, implicating the AKIT7-associated Arp2/3 complex in kinetochore-spindle attachment during chromosome segregation, although AKIT7 itself has not been directly manipulated [#2]. No direct loss-of-function experiment targeting AKIT7 has been reported in the available corpus; its role is inferred from complex membership and the phenotypes of interacting subunits.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Establishing that AKIT7 is a bona fide kinetochore component answered whether this uncharacterized apicomplexan protein has a defined subcellular assignment, placing it within the parasite's divergent kinetochore machinery.\",\n      \"evidence\": \"Interactomics (IP-MS) plus deep homology searches with microscopy-based localization\",\n      \"pmids\": [\"36006241\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct functional manipulation of AKIT7 to test its requirement for kinetochore assembly\",\n        \"Which of the four kinetochore compartments AKIT7 occupies and its direct binding partners within them are not resolved\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"},\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Co-variation of AKIT7 with ARK2 and EB1 in pulldown data addressed which spindle-associated structures AKIT7 belongs to, linking it to the ARK2/EB1 kinetochore-spindle module during gametocyte development.\",\n      \"evidence\": \"EB1-GFP and ARK2-GFP co-IP mass spectrometry with PCA of spectral counts in gametocytes\",\n      \"pmids\": [\"37704606\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Co-variation in PCA is an indirect association, not a demonstrated physical interaction\",\n        \"No direct manipulation of AKIT7 to test functional relevance of the ARK2/EB1 association\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Identifying AKIT7 as an ARPC1 interactor and observing kinetochore detachment upon ARPC1 loss addressed how kinetochores couple to spindles, implicating the AKIT7-associated Arp2/3 complex in this attachment specifically during activated male gametogenesis.\",\n      \"evidence\": \"ARPC1-GFP co-IP/MS with activation-dependent controls (P. berghei); ARPC1 genetic disruption with kinetochore imaging and AlphaFold prediction\",\n      \"pmids\": [\"40514561\", \"40828051\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Kinetochore-attachment function is inferred from the ARPC1 knockout phenotype, not from disrupting AKIT7 itself\",\n        \"Whether the AKIT7-ARPC1 association is direct or bridged by other subunits is unknown\",\n        \"The molecular basis of AKIT7's activation-dependent recruitment is not defined\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Detection of AKIT7 in the ARK1 interactome under cross-linking and its stage-biased association with SKA/PP1 modules addressed when AKIT7 engages checkpoint/kinase machinery, pointing to preferential engagement during meiosis versus male mitosis.\",\n      \"evidence\": \"Native and cross-linked ARK1-3xHA IP-MS compared across meiotic and mitotic stages (P. berghei)\",\n      \"pmids\": [\"42323312\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Interaction detected only under high cross-linking, indicating a labile or indirect association\",\n        \"The MAD1/spindle-assembly-checkpoint homology annotation is not backed by functional checkpoint assays on AKIT7\",\n        \"No direct functional manipulation of AKIT7 in meiosis versus mitosis\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether AKIT7 is itself required for kinetochore assembly, kinetochore-spindle attachment, or spindle checkpoint function remains unresolved, as all functional inferences to date rest on partner phenotypes rather than direct AKIT7 loss-of-function.\",\n      \"evidence\": \"No direct genetic disruption or depletion of AKIT7 reported in the corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No conditional knockout or knockdown of AKIT7 itself\",\n        \"No structural or biochemical demonstration of a direct AKIT7 binding partner\",\n        \"MAD1/checkpoint activity attributed to AKIT7 by homology remains functionally untested\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0000776\", \"supporting_discovery_ids\": [0, 2]},\n      {\"term_id\": \"GO:0005819\", \"supporting_discovery_ids\": [2, 4]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0007059\", \"supporting_discovery_ids\": [2]},\n      {\"term_id\": \"GO:0000070\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"complexes\": [\n      \"Apicomplexan multi-compartment kinetochore assembly\",\n      \"Atypical Plasmodium Arp2/3 complex (via ARPC1, activation-dependent)\"\n    ],\n    \"partners\": [\n      \"ARPC1 (Arp2/3 subunit)\",\n      \"ARK1 (cross-linking conditions)\",\n      \"ARK2\",\n      \"EB1\",\n      \"SKA/PP1 modules\"\n    ],\n    \"other_free_text\": [\n      \"Annotated as a MAD1 (spindle assembly checkpoint) homolog by homology only; no direct checkpoint function demonstrated for AKIT7 [#3]\",\n      \"No direct loss-of-function experiment targeting AKIT7 itself has been reported in the corpus; functional roles are inferred from ARPC1 knockout phenotypes and complex membership [#2]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0410900","name":null,"product":"apicomplexan kinetochore protein 7, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0612300","name":null,"product":"conserved Plasmodium protein, unknown function","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0309000","name":null,"product":"apicomplexan kinetochore protein 7, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AKIT7","evaluation":null}