{"gene":"PF3D7_0410800","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2022,"finding":"AKIT3 (PF3D7_0410800) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, placing it as a member of the apicomplexan kinetochore complex.","method":"Interactomics (co-immunoprecipitation/proximity labeling) combined with deep homology searches; subkinetochore localization analysis","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — interactomics-based discovery in a single study; AKIT3 is identified as a kinetochore component but no specific functional experiments are described for this individual protein beyond complex membership","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]}],"current_model":"AKIT3 (PF3D7_0410800) is established as a component of the apicomplexan kinetochore complex, identified through interactomics and deep homology searches, but no specific enzymatic activity, binding partners, or individual functional role beyond kinetochore complex membership has been mechanistically characterized."},"narrative":{"mechanistic_narrative":"AKIT3 (PF3D7_0410800) is a component of the apicomplexan kinetochore complex, identified as one of 13 previously unknown kinetochore proteins through interactomics combined with deep homology searches in Plasmodium falciparum and Plasmodium berghei [PMID:36006241]. Beyond its assignment to the kinetochore complex and subkinetochore localization, no specific enzymatic activity, individual binding partners, or discrete functional role for AKIT3 itself has been characterized in the available corpus [PMID:36006241].","teleology":[{"year":2022,"claim":"The composition of the apicomplexan kinetochore was largely unknown; systematic interactomics and homology searching established AKIT3 as a bona fide kinetochore component, placing it within the machinery that connects chromosomes to spindle microtubules during parasite division.","evidence":"Interactomics (co-immunoprecipitation/proximity labeling) combined with deep homology searches and subkinetochore localization analysis in P. falciparum and P. berghei","pmids":["36006241"],"confidence":"Medium","gaps":["No specific molecular activity or enzymatic function assigned to AKIT3 individually","No direct binding partners or substructural position within the kinetochore defined for AKIT3 alone","No loss-of-function phenotype demonstrating an individual functional requirement for AKIT3"],"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"It remains unknown what specific molecular role AKIT3 plays within the kinetochore, including its direct interaction partners, its position in the kinetochore substructure, and whether it is individually required for chromosome segregation.","evidence":"No further experimental characterization of AKIT3 is present in the available corpus","pmids":[],"confidence":"Medium","gaps":["No dedicated functional or structural study of AKIT3 as an individual protein","No reciprocal validation of AKIT3-specific interactions","No conditional knockdown/knockout data isolating AKIT3 function"],"attribution":[{"species":"Plasmodium falciparum","strain":""},{"species":"Plasmodium berghei","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Apicomplexan kinetochore complex"],"partners":[],"other_free_text":["Identified as one of 13 previously unknown apicomplexan kinetochore components via interactomics and deep homology searches [#0].","No AKIT3-specific molecular activity, binding partner, or individual functional role beyond kinetochore complex membership has been characterized in the corpus."]}},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":31,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["curated_ref"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"41820854","id":"","title":"Differential expression of mitochondria-associated genes in clinical samples of Plasmodium falciparum showing severe manifestations.","date":"2026","source":"BMC genomics","url":"https://pubmed.ncbi.nlm.nih.gov/41820854","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["AKIT3"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":4371,"output_tokens":377,"usd":0.009384,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4863,"output_tokens":1132,"usd":0.026308,"stage2_stop_reason":"end_turn"},"total_usd":0.035692,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"AKIT3 (PF3D7_0410800) was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics combined with deep homology searches, placing it as a member of the apicomplexan kinetochore complex.\",\n      \"method\": \"Interactomics (co-immunoprecipitation/proximity labeling) combined with deep homology searches; subkinetochore localization analysis\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — interactomics-based discovery in a single study; AKIT3 is identified as a kinetochore component but no specific functional experiments are described for this individual protein beyond complex membership\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"AKIT3 (PF3D7_0410800) is established as a component of the apicomplexan kinetochore complex, identified through interactomics and deep homology searches, but no specific enzymatic activity, binding partners, or individual functional role beyond kinetochore complex membership has been mechanistically characterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AKIT3 (PF3D7_0410800) is a component of the apicomplexan kinetochore complex, identified as one of 13 previously unknown kinetochore proteins through interactomics combined with deep homology searches in Plasmodium falciparum and Plasmodium berghei [#0]. Beyond its assignment to the kinetochore complex and subkinetochore localization, no specific enzymatic activity, individual binding partners, or discrete functional role for AKIT3 itself has been characterized in the available corpus [#0].\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"The composition of the apicomplexan kinetochore was largely unknown; systematic interactomics and homology searching established AKIT3 as a bona fide kinetochore component, placing it within the machinery that connects chromosomes to spindle microtubules during parasite division.\",\n      \"evidence\": \"Interactomics (co-immunoprecipitation/proximity labeling) combined with deep homology searches and subkinetochore localization analysis in P. falciparum and P. berghei\",\n      \"pmids\": [\"36006241\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No specific molecular activity or enzymatic function assigned to AKIT3 individually\",\n        \"No direct binding partners or substructural position within the kinetochore defined for AKIT3 alone\",\n        \"No loss-of-function phenotype demonstrating an individual functional requirement for AKIT3\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown what specific molecular role AKIT3 plays within the kinetochore, including its direct interaction partners, its position in the kinetochore substructure, and whether it is individually required for chromosome segregation.\",\n      \"evidence\": \"No further experimental characterization of AKIT3 is present in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No dedicated functional or structural study of AKIT3 as an individual protein\",\n        \"No reciprocal validation of AKIT3-specific interactions\",\n        \"No conditional knockdown/knockout data isolating AKIT3 function\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Plasmodium falciparum\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0000776\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0007059\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"complexes\": [\"Apicomplexan kinetochore complex\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as one of 13 previously unknown apicomplexan kinetochore components via interactomics and deep homology searches [#0].\",\n      \"No AKIT3-specific molecular activity, binding partner, or individual functional role beyond kinetochore complex membership has been characterized in the corpus.\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0410800","name":null,"product":"apicomplexan kinetochore protein 3, putative","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_0612200","name":null,"product":"conserved Plasmodium protein, unknown function","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0308900","name":null,"product":"apicomplexan kinetochore protein 3, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"AKIT3","evaluation":null}