{"gene":"PF3D7_0405200","run_date":"2026-09-08T16:26:55","timeline":{"discoveries":[{"year":2022,"finding":"CERLI2 (PF3D7_0405200) localises to the cytosolic face of the rhoptry bulb membrane in P. falciparum merozoites, as determined by cell biological analysis.","method":"Subcellular fractionation and fluorescence microscopy (direct localization experiment)","journal":"Communications biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct localization experiment with functional context, single lab, two orthogonal methods (microscopy + fractionation implied by cell biological analysis)","pmids":["35140336"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"finding":"Inducible knockdown of CERLI2 (PF3D7_0405200) inhibits blood-stage parasite growth and causes a proportion of merozoites to fail to invade erythrocytes, establishing CERLI2 as essential for erythrocyte invasion.","method":"Inducible knockdown (conditional loss-of-function) with invasion assay readout","journal":"Communications biology","confidence":"High","confidence_rationale":"Tier 2 / Moderate — clean inducible knockdown with defined cellular phenotype (invasion failure), single lab but multiple orthogonal readouts (growth assay, invasion assay, microscopy)","pmids":["35140336"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"finding":"Knockdown of CERLI2 (PF3D7_0405200) causes elongation of the rhoptry organelle during merozoite development and inhibition of rhoptry antigen processing, indicating CERLI2 has a key role in rhoptry biogenesis and antigen processing.","method":"Inducible knockdown combined with microscopy (rhoptry morphology) and biochemical analysis of rhoptry antigen processing","journal":"Communications biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — knockdown with defined morphological and biochemical phenotypes, single lab, two orthogonal readout methods","pmids":["35140336"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"finding":"Phylogenetic analysis shows that cerli2 (PF3D7_0405200) arose through an ancestral gene duplication of cerli1, indicating the two paralogs share a common evolutionary origin.","method":"Phylogenetic analysis","journal":"Communications biology","confidence":"Low","confidence_rationale":"Tier 4 / Weak — computational/phylogenetic analysis only, single study","pmids":["35140336"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"finding":"Loss of CERLI2 (PF3D7_0405200) causes morphological alterations in the rhoptry necks, as observed by ultrastructure expansion microscopy (U-ExM), distinguishing its biogenesis phenotype from that of RON11 (which causes single-rhoptry formation without structural change).","method":"Ultrastructure expansion microscopy (U-ExM) following loss-of-function","journal":"PLoS biology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — high-resolution U-ExM imaging with loss-of-function, single lab, finding referenced in a different study's comparative context","pmids":["39292724"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2020,"finding":"CERLI2 (PF3D7_0405200) was identified as a protein with similar structure and shared predicted epitopes to PfCERLI1, suggesting possible functional redundancy; it had previously been implicated in merozoite invasion.","method":"Bioinformatic sequence/structure comparison","journal":"Nature communications","confidence":"Low","confidence_rationale":"Tier 4 / Weak — computational prediction only, no direct experiment on CERLI2 in this paper","pmids":["32179747"],"is_preprint":false,"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"finding":"The AP2 transcription factor PbSIP2 in P. berghei directly binds near the cerli2 locus (ChIP-seq peak within 1200 bp of start codon), identifying cerli2 as a transcriptional target of PbSIP2 during schizogony.","method":"ChIP-seq (chromatin immunoprecipitation followed by sequencing)","journal":"Science advances","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — ChIP-seq is a direct binding assay, single lab, single method, finding is about upstream regulation of CERLI2 rather than its own mechanism","pmids":["40117352"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"CERLI2 (PF3D7_0405200) is a rhoptry bulb protein that localises to the cytosolic face of the rhoptry bulb membrane in P. falciparum merozoites; it is essential for erythrocyte invasion, and its loss causes elongation of rhoptry morphology and inhibition of rhoptry antigen processing, indicating a key role in rhoptry biogenesis and function during merozoite invasion."},"narrative":{"mechanistic_narrative":"CERLI2 (PF3D7_0405200) is a rhoptry bulb protein essential for erythrocyte invasion during the blood stage of Plasmodium falciparum [PMID:35140336]. It localises to the cytosolic face of the rhoptry bulb membrane in merozoites [PMID:35140336]. Inducible knockdown inhibits blood-stage growth and causes a proportion of merozoites to fail to invade erythrocytes, alongside elongation of the rhoptry organelle and impaired rhoptry antigen processing, placing CERLI2 in rhoptry biogenesis and function during invasion [PMID:35140336]. High-resolution ultrastructure expansion microscopy resolves the biogenesis defect to morphological alterations of the rhoptry necks, distinguishing CERLI2 from other rhoptry biogenesis factors [PMID:39292724]. In P. berghei, the cerli2 locus is a direct transcriptional target of the AP2 factor PbSIP2 during schizogony [PMID:40117352]. Beyond these findings, no molecular activity, direct protein partners, or structural mechanism for CERLI2 has been characterized in the available corpus.","teleology":[{"year":2020,"claim":"Before direct functional work, it was unknown whether CERLI2 was a distinct player in invasion; bioinformatic comparison flagged it as structurally similar to PfCERLI1 with shared predicted epitopes, raising the question of functional redundancy and prior implication in merozoite invasion.","evidence":"Bioinformatic sequence/structure comparison in P. falciparum","pmids":["32179747"],"confidence":"Low","gaps":["Computational prediction only, no direct experiment on CERLI2 in this paper","Structural similarity does not establish shared or independent function","No localization or loss-of-function data"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"To place CERLI2 in the invasion machinery, its subcellular position was resolved, establishing it as a peripheral rhoptry bulb protein facing the cytosol rather than a lumenal or secreted factor.","evidence":"Subcellular fractionation and fluorescence microscopy in P. falciparum merozoites","pmids":["35140336"],"confidence":"Medium","gaps":["Membrane anchoring mechanism not defined","No direct binding partners at the rhoptry membrane identified","Single lab"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"The question of whether CERLI2 is required for invasion was answered by conditional depletion, which arrested blood-stage growth and blocked a proportion of merozoites from invading erythrocytes, defining CERLI2 as essential.","evidence":"Inducible knockdown with growth and invasion assays in P. falciparum","pmids":["35140336"],"confidence":"High","gaps":["Step of invasion that fails is not resolved","Molecular function during invasion unknown","Partial (proportion of merozoites) invasion failure not fully explained"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2022,"claim":"To determine why invasion fails, knockdown phenotypes linked CERLI2 to organelle formation: rhoptries became elongated and antigen processing was inhibited, implicating CERLI2 in rhoptry biogenesis and maturation rather than invasion motility alone.","evidence":"Inducible knockdown with microscopy and biochemical analysis of antigen processing in P. falciparum","pmids":["35140336"],"confidence":"Medium","gaps":["Molecular basis of the morphology and processing defect unknown","Which antigens/proteases are affected not defined","Direct versus indirect role in processing unresolved"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2024,"claim":"Higher-resolution imaging refined the biogenesis defect, showing CERLI2 loss alters rhoptry neck morphology, distinguishing its phenotype from RON11 (single-rhoptry formation without structural change).","evidence":"Ultrastructure expansion microscopy (U-ExM) following loss-of-function in P. falciparum","pmids":["39292724"],"confidence":"Medium","gaps":["Mechanistic link between a cytosolic bulb protein and neck morphology not established","Comparative context drawn from a different study","Single lab"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]},{"year":2025,"claim":"The upstream control of cerli2 expression was addressed, showing the AP2 factor PbSIP2 binds near the cerli2 locus during schizogony in P. berghei, placing cerli2 within a transcriptional program timed to organelle biogenesis.","evidence":"ChIP-seq in P. berghei during schizogony","pmids":["40117352"],"confidence":"Medium","gaps":["Binding proximity does not prove functional regulation of cerli2 expression","Shown in P. berghei, not P. falciparum","Consequence of PbSIP2 loss on cerli2 not measured"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":null,"claim":"The molecular activity of CERLI2 and its direct binding partners at the rhoptry bulb membrane remain undefined, leaving open how a cytosolic-facing bulb protein controls rhoptry neck morphology and antigen processing.","evidence":"No discovery in the corpus assigns a biochemical activity or a direct interactor to CERLI2","pmids":[],"confidence":"Low","gaps":["No enzymatic or biochemical activity assigned","No experimentally validated direct protein partners","No structural model of the protein"],"attribution":[{"species":"Plasmodium falciparum","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":[],"other_free_text":["Essential for blood-stage growth and merozoite invasion of erythrocytes in P. falciparum [#1]","Loss causes rhoptry elongation and impaired rhoptry antigen processing [#2]","Loss alters rhoptry neck morphology by U-ExM, distinct from RON11 phenotype [#4]","Arose by ancestral gene duplication of cerli1 (Low confidence, phylogenetic inference) [#3]","Structurally similar to PfCERLI1 with shared predicted epitopes (Low confidence, bioinformatic) [#5]","cerli2 locus is a direct binding target of AP2 factor PbSIP2 during schizogony in P. berghei [#6]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"35140336","id":"PMC_35140336","title":"Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites.","date":"2022","source":"Communications biology","url":"https://pubmed.ncbi.nlm.nih.gov/35140336","citation_count":12,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CERLI2","PfCERLI2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"40117352","id":"","title":"SIP2 is the master transcription factor of Plasmodium merozoite formation.","date":"2025","source":"Science advances","url":"https://pubmed.ncbi.nlm.nih.gov/40117352","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CERLI2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39292724","id":"","title":"Plasmodium RON11 triggers biogenesis of the merozoite rhoptry pair and is essential for erythrocyte invasion.","date":"2024","source":"PLoS biology","url":"https://pubmed.ncbi.nlm.nih.gov/39292724","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CERLI2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"32179747","id":"","title":"PfCERLI1 is a conserved rhoptry associated protein essential for Plasmodium falciparum merozoite invasion of erythrocytes.","date":"2020","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/32179747","citation_count":0,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["CERLI2"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":6079,"output_tokens":1618,"usd":0.021254,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6696,"output_tokens":2726,"usd":0.050815,"stage2_stop_reason":"end_turn"},"total_usd":0.072069,"stage1_batch_id":"msgbatch_01NE1MTa8x1hW7Lo4tAaNzY1","stage2_batch_id":"msgbatch_0141vaQdeFvbo2q39AinvXM9","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"CERLI2 (PF3D7_0405200) localises to the cytosolic face of the rhoptry bulb membrane in P. falciparum merozoites, as determined by cell biological analysis.\",\n      \"method\": \"Subcellular fractionation and fluorescence microscopy (direct localization experiment)\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization experiment with functional context, single lab, two orthogonal methods (microscopy + fractionation implied by cell biological analysis)\",\n      \"pmids\": [\"35140336\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"Inducible knockdown of CERLI2 (PF3D7_0405200) inhibits blood-stage parasite growth and causes a proportion of merozoites to fail to invade erythrocytes, establishing CERLI2 as essential for erythrocyte invasion.\",\n      \"method\": \"Inducible knockdown (conditional loss-of-function) with invasion assay readout\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean inducible knockdown with defined cellular phenotype (invasion failure), single lab but multiple orthogonal readouts (growth assay, invasion assay, microscopy)\",\n      \"pmids\": [\"35140336\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"Knockdown of CERLI2 (PF3D7_0405200) causes elongation of the rhoptry organelle during merozoite development and inhibition of rhoptry antigen processing, indicating CERLI2 has a key role in rhoptry biogenesis and antigen processing.\",\n      \"method\": \"Inducible knockdown combined with microscopy (rhoptry morphology) and biochemical analysis of rhoptry antigen processing\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — knockdown with defined morphological and biochemical phenotypes, single lab, two orthogonal readout methods\",\n      \"pmids\": [\"35140336\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"Phylogenetic analysis shows that cerli2 (PF3D7_0405200) arose through an ancestral gene duplication of cerli1, indicating the two paralogs share a common evolutionary origin.\",\n      \"method\": \"Phylogenetic analysis\",\n      \"journal\": \"Communications biology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — computational/phylogenetic analysis only, single study\",\n      \"pmids\": [\"35140336\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"Loss of CERLI2 (PF3D7_0405200) causes morphological alterations in the rhoptry necks, as observed by ultrastructure expansion microscopy (U-ExM), distinguishing its biogenesis phenotype from that of RON11 (which causes single-rhoptry formation without structural change).\",\n      \"method\": \"Ultrastructure expansion microscopy (U-ExM) following loss-of-function\",\n      \"journal\": \"PLoS biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — high-resolution U-ExM imaging with loss-of-function, single lab, finding referenced in a different study's comparative context\",\n      \"pmids\": [\"39292724\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"CERLI2 (PF3D7_0405200) was identified as a protein with similar structure and shared predicted epitopes to PfCERLI1, suggesting possible functional redundancy; it had previously been implicated in merozoite invasion.\",\n      \"method\": \"Bioinformatic sequence/structure comparison\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — computational prediction only, no direct experiment on CERLI2 in this paper\",\n      \"pmids\": [\"32179747\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"The AP2 transcription factor PbSIP2 in P. berghei directly binds near the cerli2 locus (ChIP-seq peak within 1200 bp of start codon), identifying cerli2 as a transcriptional target of PbSIP2 during schizogony.\",\n      \"method\": \"ChIP-seq (chromatin immunoprecipitation followed by sequencing)\",\n      \"journal\": \"Science advances\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — ChIP-seq is a direct binding assay, single lab, single method, finding is about upstream regulation of CERLI2 rather than its own mechanism\",\n      \"pmids\": [\"40117352\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"CERLI2 (PF3D7_0405200) is a rhoptry bulb protein that localises to the cytosolic face of the rhoptry bulb membrane in P. falciparum merozoites; it is essential for erythrocyte invasion, and its loss causes elongation of rhoptry morphology and inhibition of rhoptry antigen processing, indicating a key role in rhoptry biogenesis and function during merozoite invasion.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"CERLI2 (PF3D7_0405200) is a rhoptry bulb protein essential for erythrocyte invasion during the blood stage of Plasmodium falciparum [#1]. It localises to the cytosolic face of the rhoptry bulb membrane in merozoites [#0]. Inducible knockdown inhibits blood-stage growth and causes a proportion of merozoites to fail to invade erythrocytes, alongside elongation of the rhoptry organelle and impaired rhoptry antigen processing, placing CERLI2 in rhoptry biogenesis and function during invasion [#1, #2]. High-resolution ultrastructure expansion microscopy resolves the biogenesis defect to morphological alterations of the rhoptry necks, distinguishing CERLI2 from other rhoptry biogenesis factors [#4]. In P. berghei, the cerli2 locus is a direct transcriptional target of the AP2 factor PbSIP2 during schizogony [#6]. Beyond these findings, no molecular activity, direct protein partners, or structural mechanism for CERLI2 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2020,\n      \"claim\": \"Before direct functional work, it was unknown whether CERLI2 was a distinct player in invasion; bioinformatic comparison flagged it as structurally similar to PfCERLI1 with shared predicted epitopes, raising the question of functional redundancy and prior implication in merozoite invasion.\",\n      \"evidence\": \"Bioinformatic sequence/structure comparison in P. falciparum\",\n      \"pmids\": [\"32179747\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"Computational prediction only, no direct experiment on CERLI2 in this paper\", \"Structural similarity does not establish shared or independent function\", \"No localization or loss-of-function data\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"To place CERLI2 in the invasion machinery, its subcellular position was resolved, establishing it as a peripheral rhoptry bulb protein facing the cytosol rather than a lumenal or secreted factor.\",\n      \"evidence\": \"Subcellular fractionation and fluorescence microscopy in P. falciparum merozoites\",\n      \"pmids\": [\"35140336\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Membrane anchoring mechanism not defined\", \"No direct binding partners at the rhoptry membrane identified\", \"Single lab\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"The question of whether CERLI2 is required for invasion was answered by conditional depletion, which arrested blood-stage growth and blocked a proportion of merozoites from invading erythrocytes, defining CERLI2 as essential.\",\n      \"evidence\": \"Inducible knockdown with growth and invasion assays in P. falciparum\",\n      \"pmids\": [\"35140336\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Step of invasion that fails is not resolved\", \"Molecular function during invasion unknown\", \"Partial (proportion of merozoites) invasion failure not fully explained\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"To determine why invasion fails, knockdown phenotypes linked CERLI2 to organelle formation: rhoptries became elongated and antigen processing was inhibited, implicating CERLI2 in rhoptry biogenesis and maturation rather than invasion motility alone.\",\n      \"evidence\": \"Inducible knockdown with microscopy and biochemical analysis of antigen processing in P. falciparum\",\n      \"pmids\": [\"35140336\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Molecular basis of the morphology and processing defect unknown\", \"Which antigens/proteases are affected not defined\", \"Direct versus indirect role in processing unresolved\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Higher-resolution imaging refined the biogenesis defect, showing CERLI2 loss alters rhoptry neck morphology, distinguishing its phenotype from RON11 (single-rhoptry formation without structural change).\",\n      \"evidence\": \"Ultrastructure expansion microscopy (U-ExM) following loss-of-function in P. falciparum\",\n      \"pmids\": [\"39292724\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Mechanistic link between a cytosolic bulb protein and neck morphology not established\", \"Comparative context drawn from a different study\", \"Single lab\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"The upstream control of cerli2 expression was addressed, showing the AP2 factor PbSIP2 binds near the cerli2 locus during schizogony in P. berghei, placing cerli2 within a transcriptional program timed to organelle biogenesis.\",\n      \"evidence\": \"ChIP-seq in P. berghei during schizogony\",\n      \"pmids\": [\"40117352\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Binding proximity does not prove functional regulation of cerli2 expression\", \"Shown in P. berghei, not P. falciparum\", \"Consequence of PbSIP2 loss on cerli2 not measured\"],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular activity of CERLI2 and its direct binding partners at the rhoptry bulb membrane remain undefined, leaving open how a cytosolic-facing bulb protein controls rhoptry neck morphology and antigen processing.\",\n      \"evidence\": \"No discovery in the corpus assigns a biochemical activity or a direct interactor to CERLI2\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No enzymatic or biochemical activity assigned\", \"No experimentally validated direct protein partners\", \"No structural model of the protein\"],\n      \"attribution\": [{\"species\": \"Plasmodium falciparum\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"rhoptry\", \"supporting_discovery_ids\": [0, 2, 4]},\n      {\"term_id\": \"rhoptry membrane, cytosolic face\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"erythrocyte invasion by merozoite\", \"supporting_discovery_ids\": [1]},\n      {\"term_id\": \"rhoptry biogenesis\", \"supporting_discovery_ids\": [2, 4]}\n    ],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Essential for blood-stage growth and merozoite invasion of erythrocytes in P. falciparum [#1]\",\n      \"Loss causes rhoptry elongation and impaired rhoptry antigen processing [#2]\",\n      \"Loss alters rhoptry neck morphology by U-ExM, distinct from RON11 phenotype [#4]\",\n      \"Arose by ancestral gene duplication of cerli1 (Low confidence, phylogenetic inference) [#3]\",\n      \"Structurally similar to PfCERLI1 with shared predicted epitopes (Low confidence, bioinformatic) [#5]\",\n      \"cerli2 locus is a direct binding target of AP2 factor PbSIP2 during schizogony in P. berghei [#6]\"\n    ]\n  }\n}\n```","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0405200","name":null,"product":"protein CERLI2","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1002900","name":null,"product":"ag-1 blood stage membrane protein homologue","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0303300","name":null,"product":"protein CERLI2, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"CERLI2","evaluation":null}