{"gene":"PF3D7_0320800","run_date":"2026-09-07T10:55:51","timeline":{"discoveries":[{"year":2006,"finding":"DOZI (DDX6-class RNA helicase) is found in a complex with mRNA species in cytoplasmic bodies of female blood-stage gametocytes of Plasmodium berghei, forming translationally repressed ribonucleoprotein complexes that are stored for translation after fertilization. Genetic disruption of pbdozi inhibits formation of these RNP complexes and diverts at least 370 transcripts to a degradation pathway, demonstrating DOZI is required for mRNA stabilization and translational repression in gametocytes.","method":"Genetic disruption (knockout) of pbdozi combined with transcriptomic profiling; localization of DOZI to cytoplasmic bodies by imaging","journal":"Science","confidence":"High","confidence_rationale":"Tier 2 / Strong — clean genetic knockout with defined molecular phenotype (loss of RNP complexes, transcript degradation) replicated across subsequent studies","pmids":["16888139"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2010,"finding":"DOZI anchors a large messenger ribonucleoprotein (mRNP) complex in P. berghei female gametocytes, defined together with the Sm-like factor CITH, comprising 16 major factors including proteins homologous to metazoan P granule components. The complex contains translationally silent transcripts and integrates eIF4E and poly(A)-binding protein but excludes P-body RNA degradation factors and translation-initiation-promoting eIF4G. Gene deletion of DOZI produces fertilization-competent gametocytes whose zygotes fail to develop into ookinetes.","method":"Affinity purification coupled with mass spectrometry (AP-MS) to define mRNP composition; gene deletion (DOZI-KO); RNA-immunoprecipitation; global expression profiling","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 1–2 / Strong — multiple orthogonal methods (AP-MS, RNA-IP, expression profiling, genetic knockout) in a single rigorous study defining complex composition and functional consequence","pmids":["20169188"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"finding":"TurboID-based proximity proteomics of the DOZI/CITH/ALBA complex in P. yoelii female gametocytes and zygotes revealed substantial spatial and/or compositional changes in the complex across the host-to-vector transmission event, consistent with global release of translational repression of ~200 transcripts soon after fertilization while many transcripts remain repressed beyond this point.","method":"TurboID proximity proteomics; quantitative DIA-MS proteomics; comparative transcriptomics across female gametocyte and zygote stages","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — proximity proteomics plus quantitative transcriptomics and proteomics in a single study, single lab, not yet independently replicated","pmids":["39777415"],"is_preprint":false,"attribution":[{"species":"Plasmodium yoelii","strain":""}]},{"year":2012,"finding":"In P. berghei ppkl(-) mutants, mRNA expression of dozi was increased, linking the PPKL phosphatase signaling pathway to regulation of DOZI levels during ookinete differentiation.","method":"Gene deletion of ppkl followed by mRNA expression analysis of dozi","journal":"PLoS pathogens","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single indirect measurement (mRNA level change) in a study primarily focused on PPKL, not DOZI; no direct mechanistic follow-up on DOZI itself","pmids":["23028336"],"is_preprint":false,"attribution":[{"species":"Plasmodium berghei","strain":""}]}],"current_model":"DOZI (PF3D7_0320800), a DDX6-class ATP-dependent RNA helicase, forms a large translationally silent mRNP complex (the Plasmodium P granule) with CITH, eIF4E, poly(A)-binding protein, and at least 16 other factors in female gametocyte cytoplasmic bodies, where it stabilizes and translationally represses hundreds of maternal mRNAs that are stored for translation after fertilization; loss of DOZI redirects these transcripts to degradation and blocks zygote-to-ookinete development, while the complex undergoes substantial compositional and spatial reorganization upon fertilization to permit global release of translational repression."},"narrative":{"mechanistic_narrative":"DOZI (PF3D7_0320800) is a DDX6-class ATP-dependent RNA helicase that acts as the organizing hub of a translationally silent maternal mRNP in female Plasmodium gametocytes, storing transcripts for translation after fertilization [PMID:16888139, PMID:20169188]. In P. berghei, DOZI localizes to cytoplasmic bodies and anchors a large mRNP defined together with the Sm-like factor CITH; the complex comprises 16 major factors including metazoan P-granule homologs, integrates eIF4E and poly(A)-binding protein, yet excludes P-body RNA degradation factors and the translation-initiation factor eIF4G, a composition consistent with mRNA storage and repression rather than degradation or active translation [PMID:20169188]. Genetic disruption of dozi prevents formation of these RNP complexes and diverts at least 370 stored transcripts into a degradation pathway, establishing DOZI as required for both stabilization and translational repression of maternal mRNAs; dozi-null parasites produce fertilization-competent gametocytes whose zygotes fail to develop into ookinetes [PMID:16888139, PMID:20169188]. Across the host-to-vector transmission event in P. yoelii, the DOZI/CITH/ALBA complex undergoes substantial spatial and/or compositional reorganization coincident with release of translational repression of roughly 200 transcripts soon after fertilization, while many transcripts remain repressed beyond this point [PMID:39777415]. Beyond its role in this storage mRNP and a Low-confidence link between the PPKL phosphatase pathway and dozi transcript levels [PMID:23028336], no further mechanistic detail on DOZI's helicase enzymology has been characterized in the available corpus.","teleology":[{"year":2006,"claim":"It was unknown how female gametocytes preserve maternal mRNAs across fertilization; this established DOZI as the factor required to stabilize and translationally repress these transcripts rather than let them degrade.","evidence":"Knockout of pbdozi with transcriptomic profiling and imaging of DOZI in cytoplasmic bodies, in P. berghei","pmids":["16888139"],"confidence":"High","gaps":["Does not resolve whether DOZI's ATP-dependent helicase activity is directly required, versus a purely scaffolding role","Mechanism of transcript selection into the repressed pool not defined","No structural information on how DOZI engages RNA"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2010,"claim":"The molecular identity of the DOZI storage particle was unknown; defining its composition established it as a storage-specialized mRNP that excludes degradation and active-translation machinery.","evidence":"AP-MS, RNA-immunoprecipitation, gene deletion, and global expression profiling defining the DOZI/CITH complex in P. berghei female gametocytes","pmids":["20169188"],"confidence":"High","gaps":["Does not establish which subunit contacts are direct versus RNA-bridged","The specific contribution of DOZI (versus CITH or other subunits) to repression not dissected","How eIF4E is held in a non-initiating state within the complex unresolved"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2012,"claim":"Regulation of DOZI expression during development was unaddressed; loss of the PPKL phosphatase raised dozi mRNA, tentatively linking a signaling pathway to DOZI abundance during ookinete differentiation.","evidence":"ppkl gene deletion followed by mRNA analysis of dozi in P. berghei","pmids":["23028336"],"confidence":"Low","gaps":["Single indirect mRNA measurement in a PPKL-focused study, with no direct mechanistic follow-up on DOZI","Whether PPKL acts on DOZI directly or through intermediate factors unknown","Effect on DOZI protein level or complex function not tested"],"attribution":[{"species":"Plasmodium berghei","strain":""}]},{"year":2025,"claim":"How the storage mRNP is disassembled to permit translation after fertilization was unclear; proximity proteomics showed the complex reorganizes spatially/compositionally across transmission, coupling structural change to release of repression.","evidence":"TurboID proximity proteomics with DIA-MS and comparative transcriptomics across female gametocyte and zygote stages in P. yoelii","pmids":["39777415"],"confidence":"Medium","gaps":["Single lab, not yet independently replicated","Molecular trigger for complex reorganization not identified","Why a subset of transcripts remains repressed beyond fertilization unexplained"],"attribution":[{"species":"Plasmodium yoelii","strain":""}]},{"year":null,"claim":"Whether DOZI's ATP-dependent helicase catalytic activity is mechanistically required for mRNA storage, repression, or de-repression—versus a scaffolding function—remains open.","evidence":"","pmids":[],"confidence":"Low","gaps":["No catalytically-dead mutant or in vitro helicase assay in the corpus","No structural model of DOZI on RNA or within the mRNP","Direct RNA substrates of DOZI's helicase activity not defined"],"attribution":[]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["DOZI/CITH storage mRNP (Plasmodium P granule)","DOZI/CITH/ALBA complex"],"partners":["CITH (SM-LIKE FACTOR)","EIF4E","POLY(A)-BINDING PROTEIN","ALBA PROTEINS"],"other_free_text":["Complex excludes P-body RNA degradation factors and translation-initiation factor eIF4G [#1]","Loss of DOZI diverts >=370 stored transcripts to degradation and blocks zygote-to-ookinete development [#0, #1]","Complex reorganizes across host-to-vector transmission, releasing repression of ~200 transcripts after fertilization in P. yoelii [#2]","dozi mRNA increased in P. berghei ppkl(-) mutants (Low confidence) [#3]"]}},"prefetch_data":{},"corpus_meta":[{"pmid":"16888139","id":"PMC_16888139","title":"Regulation of sexual development of Plasmodium by translational repression.","date":"2006","source":"Science (New York, N.Y.)","url":"https://pubmed.ncbi.nlm.nih.gov/16888139","citation_count":380,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI","DDX6"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"10693754","id":"PMC_10693754","title":"A simple RNA analysis method shows var and rif multigene family expression patterns in Plasmodium falciparum.","date":"2000","source":"Molecular and biochemical parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/10693754","citation_count":234,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"20169188","id":"PMC_20169188","title":"Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development.","date":"2010","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/20169188","citation_count":216,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"23028336","id":"PMC_23028336","title":"A unique protein phosphatase with kelch-like domains (PPKL) in Plasmodium modulates ookinete differentiation, motility and invasion.","date":"2012","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/23028336","citation_count":76,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"26525978","id":"PMC_26525978","title":"A bioinformatic survey of RNA-binding proteins in Plasmodium.","date":"2015","source":"BMC genomics","url":"https://pubmed.ncbi.nlm.nih.gov/26525978","citation_count":62,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI","RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"33497624","id":"PMC_33497624","title":"Structure, function, and substrates of Clp AAA+ protease systems in cyanobacteria, plastids, and apicoplasts: A comparative analysis.","date":"2021","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/33497624","citation_count":61,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["ATP"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"21673790","id":"PMC_21673790","title":"The Puf-family RNA-binding protein Puf2 controls sporozoite conversion to liver stages in the malaria parasite.","date":"2011","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/21673790","citation_count":59,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI","RNA","DDX6"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"32817449","id":"PMC_32817449","title":"Redesigned TetR-Aptamer System To Control Gene Expression in Plasmodium falciparum.","date":"2020","source":"mSphere","url":"https://pubmed.ncbi.nlm.nih.gov/32817449","citation_count":55,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"29626096","id":"PMC_29626096","title":"The mitochondrial ribosomal protein L13 is critical for the structural and functional integrity of the mitochondrion in Plasmodium falciparum.","date":"2018","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/29626096","citation_count":54,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"27230797","id":"PMC_27230797","title":"Translational regulation in blood stages of the malaria parasite Plasmodium spp.: systems-wide studies pave the way.","date":"2016","source":"Wiley interdisciplinary reviews. RNA","url":"https://pubmed.ncbi.nlm.nih.gov/27230797","citation_count":54,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["RNA"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"25387887","id":"PMC_25387887","title":"Translational regulation during stage transitions in malaria parasites.","date":"2014","source":"Annals of the New York Academy of Sciences","url":"https://pubmed.ncbi.nlm.nih.gov/25387887","citation_count":50,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"31990132","id":"PMC_31990132","title":"EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole.","date":"2020","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/31990132","citation_count":47,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false},{"pmid":"39777415","id":"PMC_39777415","title":"Widespread release of translational repression across Plasmodium's host-to-vector transmission event.","date":"2025","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/39777415","citation_count":10,"is_preprint":false,"source_species":["Pf"],"role":"primary","match_terms":["DOZI"],"competing_organism_flag":false,"competing_organism_match":"","competing_organism_only":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":9198,"output_tokens":1305,"usd":0.023585,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6227,"output_tokens":2617,"usd":0.04828,"stage2_stop_reason":"end_turn"},"total_usd":0.071865,"stage1_batch_id":"msgbatch_01DbiymDmubkPpYvdbAu5a7V","stage2_batch_id":"msgbatch_012TbrWvMRH129T7PQ4SmvrJ","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2006,\n      \"finding\": \"DOZI (DDX6-class RNA helicase) is found in a complex with mRNA species in cytoplasmic bodies of female blood-stage gametocytes of Plasmodium berghei, forming translationally repressed ribonucleoprotein complexes that are stored for translation after fertilization. Genetic disruption of pbdozi inhibits formation of these RNP complexes and diverts at least 370 transcripts to a degradation pathway, demonstrating DOZI is required for mRNA stabilization and translational repression in gametocytes.\",\n      \"method\": \"Genetic disruption (knockout) of pbdozi combined with transcriptomic profiling; localization of DOZI to cytoplasmic bodies by imaging\",\n      \"journal\": \"Science\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — clean genetic knockout with defined molecular phenotype (loss of RNP complexes, transcript degradation) replicated across subsequent studies\",\n      \"pmids\": [\"16888139\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2010,\n      \"finding\": \"DOZI anchors a large messenger ribonucleoprotein (mRNP) complex in P. berghei female gametocytes, defined together with the Sm-like factor CITH, comprising 16 major factors including proteins homologous to metazoan P granule components. The complex contains translationally silent transcripts and integrates eIF4E and poly(A)-binding protein but excludes P-body RNA degradation factors and translation-initiation-promoting eIF4G. Gene deletion of DOZI produces fertilization-competent gametocytes whose zygotes fail to develop into ookinetes.\",\n      \"method\": \"Affinity purification coupled with mass spectrometry (AP-MS) to define mRNP composition; gene deletion (DOZI-KO); RNA-immunoprecipitation; global expression profiling\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1–2 / Strong — multiple orthogonal methods (AP-MS, RNA-IP, expression profiling, genetic knockout) in a single rigorous study defining complex composition and functional consequence\",\n      \"pmids\": [\"20169188\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"TurboID-based proximity proteomics of the DOZI/CITH/ALBA complex in P. yoelii female gametocytes and zygotes revealed substantial spatial and/or compositional changes in the complex across the host-to-vector transmission event, consistent with global release of translational repression of ~200 transcripts soon after fertilization while many transcripts remain repressed beyond this point.\",\n      \"method\": \"TurboID proximity proteomics; quantitative DIA-MS proteomics; comparative transcriptomics across female gametocyte and zygote stages\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — proximity proteomics plus quantitative transcriptomics and proteomics in a single study, single lab, not yet independently replicated\",\n      \"pmids\": [\"39777415\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium yoelii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2012,\n      \"finding\": \"In P. berghei ppkl(-) mutants, mRNA expression of dozi was increased, linking the PPKL phosphatase signaling pathway to regulation of DOZI levels during ookinete differentiation.\",\n      \"method\": \"Gene deletion of ppkl followed by mRNA expression analysis of dozi\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single indirect measurement (mRNA level change) in a study primarily focused on PPKL, not DOZI; no direct mechanistic follow-up on DOZI itself\",\n      \"pmids\": [\"23028336\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"DOZI (PF3D7_0320800), a DDX6-class ATP-dependent RNA helicase, forms a large translationally silent mRNP complex (the Plasmodium P granule) with CITH, eIF4E, poly(A)-binding protein, and at least 16 other factors in female gametocyte cytoplasmic bodies, where it stabilizes and translationally represses hundreds of maternal mRNAs that are stored for translation after fertilization; loss of DOZI redirects these transcripts to degradation and blocks zygote-to-ookinete development, while the complex undergoes substantial compositional and spatial reorganization upon fertilization to permit global release of translational repression.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"DOZI (PF3D7_0320800) is a DDX6-class ATP-dependent RNA helicase that acts as the organizing hub of a translationally silent maternal mRNP in female Plasmodium gametocytes, storing transcripts for translation after fertilization [#0, #1]. In P. berghei, DOZI localizes to cytoplasmic bodies and anchors a large mRNP defined together with the Sm-like factor CITH; the complex comprises 16 major factors including metazoan P-granule homologs, integrates eIF4E and poly(A)-binding protein, yet excludes P-body RNA degradation factors and the translation-initiation factor eIF4G, a composition consistent with mRNA storage and repression rather than degradation or active translation [#1]. Genetic disruption of dozi prevents formation of these RNP complexes and diverts at least 370 stored transcripts into a degradation pathway, establishing DOZI as required for both stabilization and translational repression of maternal mRNAs; dozi-null parasites produce fertilization-competent gametocytes whose zygotes fail to develop into ookinetes [#0, #1]. Across the host-to-vector transmission event in P. yoelii, the DOZI/CITH/ALBA complex undergoes substantial spatial and/or compositional reorganization coincident with release of translational repression of roughly 200 transcripts soon after fertilization, while many transcripts remain repressed beyond this point [#2]. Beyond its role in this storage mRNP and a Low-confidence link between the PPKL phosphatase pathway and dozi transcript levels [#3], no further mechanistic detail on DOZI's helicase enzymology has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2006,\n      \"claim\": \"It was unknown how female gametocytes preserve maternal mRNAs across fertilization; this established DOZI as the factor required to stabilize and translationally repress these transcripts rather than let them degrade.\",\n      \"evidence\": \"Knockout of pbdozi with transcriptomic profiling and imaging of DOZI in cytoplasmic bodies, in P. berghei\",\n      \"pmids\": [\"16888139\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Does not resolve whether DOZI's ATP-dependent helicase activity is directly required, versus a purely scaffolding role\",\n        \"Mechanism of transcript selection into the repressed pool not defined\",\n        \"No structural information on how DOZI engages RNA\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2010,\n      \"claim\": \"The molecular identity of the DOZI storage particle was unknown; defining its composition established it as a storage-specialized mRNP that excludes degradation and active-translation machinery.\",\n      \"evidence\": \"AP-MS, RNA-immunoprecipitation, gene deletion, and global expression profiling defining the DOZI/CITH complex in P. berghei female gametocytes\",\n      \"pmids\": [\"20169188\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Does not establish which subunit contacts are direct versus RNA-bridged\",\n        \"The specific contribution of DOZI (versus CITH or other subunits) to repression not dissected\",\n        \"How eIF4E is held in a non-initiating state within the complex unresolved\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2012,\n      \"claim\": \"Regulation of DOZI expression during development was unaddressed; loss of the PPKL phosphatase raised dozi mRNA, tentatively linking a signaling pathway to DOZI abundance during ookinete differentiation.\",\n      \"evidence\": \"ppkl gene deletion followed by mRNA analysis of dozi in P. berghei\",\n      \"pmids\": [\"23028336\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single indirect mRNA measurement in a PPKL-focused study, with no direct mechanistic follow-up on DOZI\",\n        \"Whether PPKL acts on DOZI directly or through intermediate factors unknown\",\n        \"Effect on DOZI protein level or complex function not tested\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"How the storage mRNP is disassembled to permit translation after fertilization was unclear; proximity proteomics showed the complex reorganizes spatially/compositionally across transmission, coupling structural change to release of repression.\",\n      \"evidence\": \"TurboID proximity proteomics with DIA-MS and comparative transcriptomics across female gametocyte and zygote stages in P. yoelii\",\n      \"pmids\": [\"39777415\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Single lab, not yet independently replicated\",\n        \"Molecular trigger for complex reorganization not identified\",\n        \"Why a subset of transcripts remains repressed beyond fertilization unexplained\"\n      ],\n      \"attribution\": [{\"species\": \"Plasmodium yoelii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether DOZI's ATP-dependent helicase catalytic activity is mechanistically required for mRNA storage, repression, or de-repression—versus a scaffolding function—remains open.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No catalytically-dead mutant or in vitro helicase assay in the corpus\",\n        \"No structural model of DOZI on RNA or within the mRNP\",\n        \"Direct RNA substrates of DOZI's helicase activity not defined\"\n      ],\n      \"attribution\": []\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003724\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0017148\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0003729\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0036464\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0005737\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006417\", \"supporting_discovery_ids\": [0, 1, 2]},\n      {\"term_id\": \"GO:0048315\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"complexes\": [\n      \"DOZI/CITH storage mRNP (Plasmodium P granule)\",\n      \"DOZI/CITH/ALBA complex\"\n    ],\n    \"partners\": [\n      \"CITH (Sm-like factor)\",\n      \"eIF4E\",\n      \"poly(A)-binding protein\",\n      \"ALBA proteins\"\n    ],\n    \"other_free_text\": [\n      \"Complex excludes P-body RNA degradation factors and translation-initiation factor eIF4G [#1]\",\n      \"Loss of DOZI diverts >=370 stored transcripts to degradation and blocks zygote-to-ookinete development [#0, #1]\",\n      \"Complex reorganizes across host-to-vector transmission, releasing repression of ~200 transcripts after fertilization in P. yoelii [#2]\",\n      \"dozi mRNA increased in P. berghei ppkl(-) mutants (Low confidence) [#3]\"\n    ]\n  }\n}","audit_flag":null,"strains":[{"strain":"Pf3D7","gene_id":"PF3D7_0320800","name":null,"product":"ATP-dependent RNA helicase DDX6","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PbANKA","gene_id":"PBANKA_1217700","name":null,"product":"ATP-dependent RNA helicase DDX6","uniprot_acc":null,"chromosome":null,"start_pos":null},{"strain":"PkH","gene_id":"PKNH_0820000","name":null,"product":"ATP-dependent RNA helicase DDX6, putative","uniprot_acc":null,"chromosome":null,"start_pos":null}],"gene_symbol":"DOZI","evaluation":null}